Abstract:
:Met protein is a tyrosine kinase receptor for hepatocyte growth factor (HGF). c-Met has morphogenic, mitogenic, and motogenic properties and is overexpressed in many solid tumors. We studied c-met mRNA and protein expression in papillary thyroid carcinomas and nonneoplastic thyroid tissue. The c-met mRNA was detected in all biopsies by reverse transcriptase-polymerase chain reaction and by hybridization of complex cDNA probes to a c-met-specific DNA fragment in a dot blot array. Immunohistochemistry on fresh frozen biopsies showed Met protein localized along the basal cell membrane of normal thyrocytes in 32 of 35 nonneoplastic thyroid tissue specimens, sometimes associated with weak cytoplasmic reactivity but without apical cell membrane staining. In papillary carcinomas an increased Met protein expression was seen, comprising a cytoplasmic (33 of 49) and apical cell membrane (24 of 49) immunoreactivity, whereas only 1 of 49 biopsies showed basal cell membrane staining. A 145-kDa Met-specific band was detected by Western immunoblotting on protein extracts from papillary carcinomas. The tight junction protein zona occludens-1 (ZO-1), studied by immunohistochemistry, was weakly expressed along the apical cell membrane in 10 nonneoplastic biopsies. In contrast, increased and cytoplasmic/apical membranous ZO-1 immunostaining was seen in 11 of 15 papillary carcinomas. Nuclear ZO-1 staining was present in a few papillary carcinomas with partial dedifferentiation. The concomitant overexpression and subcellular redistribution of Met and ZO-1 proteins indicate a change in cell polarity in papillary carcinomas compared to nonneoplastic thyroid tissue. These observations may reflect an important feature of the tumorigenesis of papillary thyroid carcinomas. No significant association was found between semiquantitative immunohistochemical assessment of Met protein and clinical parameters in papillary carcinoma patients.
journal_name
World J Surgjournal_title
World journal of surgeryauthors
Fluge Ø,Haugen DR,Lillehaug JR,Varhaug JEdoi
10.1007/s002680020167keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
623-31issue
5eissn
0364-2313issn
1432-2323journal_volume
25pub_type
杂志文章abstract:BACKGROUND:The quality of surgical performance depends on the technical skills of the surgical team as well as on non-technical skills, including teamwork. The present study evaluated the impact of familiarity among members of the surgical team on morbidity in patients undergoing elective open abdominal surgery. METHO...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-014-2680-2
更新日期:2014-12-01 00:00:00
abstract::Primary hyperparathyroidism was investigated using the presence of basic fibroblast growth factor (bFGF) from the immunohistochemical viewpoint with an anti-bFGF antibody in hyperplastic parathyroid glands of patients with multiple endocrine neoplasia type I (MEN-I) and of patients with non-MEN. The results correspond...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF00299109
更新日期:1994-11-01 00:00:00
abstract::Controversy remains regarding the best surgical approach for toxic multinodular goiter (MNG). The aim of this study was to evaluate the results of various thyroid operations for managing toxic MNG. A group of 100 patients with toxic MNG were divided into three groups and managed with total thyroidectomy (TT; group I, ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-005-7767-3
更新日期:2005-07-01 00:00:00
abstract::A group of 105 consecutive patients with venographically proved major acute deep vein thrombosis (DVT) were randomized in an open prospective study to evaluate the comparative efficacy and safety of a fixed dose of subcutaneous low-molecular-weight heparin (LMWH) and warfarin for the prevention of recurrent venous thr...
journal_title:World journal of surgery
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s002689900081
更新日期:1996-06-01 00:00:00
abstract::Malnutrition in its various forms is without doubt the commonest disease. Acute malnutrition resulting from a total lack of ingestion clearly shows that not only are water and electrolytes a priority for survival but also energy reserves. It also shows that the maximum wasting that the lean body mass can withstand is ...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/pl00012343
更新日期:1999-06-01 00:00:00
abstract:INTRODUCTION:An increasing body of evidence is being published about single-incision laparoscopic cholecystectomy (SILC), but there are no well-powered trials with an adequate evaluation of post-operative pain. This randomized trial compares SILC against four-port laparoscopic cholecystectomy (LC) with post-operative p...
journal_title:World journal of surgery
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00268-014-2903-6
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:The aim of the present study was to evaluate the influence of donor age on liver regeneration and surgical outcomes in recipients and donors. PATIENTS AND METHODS:Among 101 cases of adult-to-adult living donor liver transplantation (LDLT) between March 2002 and March 2011, according to donor age: younger (Y...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-012-1496-1
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:Skeletal muscle depletion, referred to as sarcopenia, has been shown to be an independent predictor of lower disease-free and overall survivals in various kinds of diseases. The quality of skeletal muscle has recently attracted much attention as a new parameter of sarcopenia, but its impact on surgical compl...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-016-3504-3
更新日期:2016-08-01 00:00:00
abstract::In 1947, a patient with metastatic islet cell tumor was treated for intractable ulcer disease at the University of Chicago Medical Center. Eight years later, in retrospect, it was recognized that he and another patient had the Zollinger-Ellison syndrome (ZE). From 1947 until the present, 30 patients with the ZE syndro...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF01658530
更新日期:1990-05-01 00:00:00
abstract:BACKGROUND:Molecular diagnostics can allow some patients with indeterminate thyroid nodule cytopathology to avoid diagnostic hemithyroidectomy; however, the testing is costly. We hypothesized that molecular testing with the intention of preventing unnecessary diagnostic hemithyroidectomy would be cost-effective if this...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-019-05177-7
更新日期:2020-02-01 00:00:00
abstract::In 1989-90, all 37 lung cancer patients scheduled for surgery underwent transesophageal endoscopic ultrasonography (EUS) for pre-operative detection of hilar and mediastinal lymph node metastases. An electronic ultrasonic fiberscope with a linear array (EPB-503-FS, Machida-Toshiba) was used. Of 380 nodes surgically re...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF02067013
更新日期:1992-09-01 00:00:00
abstract::Postoperative complications in patients with obstructive jaundice remain increased when associated with endotoxemia and the inflammatory response due to gut barrier failure. Administration of glutamine has been proposed to maintain the integrity of the gut mucosa and thus reduce bacterial translocation (BT), but the e...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-005-7721-4
更新日期:2005-10-01 00:00:00
abstract::We retrospectively reviewed postoperative complications in 105 patients with hilar cholangiocarcinoma who underwent hepatectomy at Nagoya University Hospital from January 1990 through March 1999. Of the 105 subjects, 97 (92.4%) underwent resection of two or more Healey's segments of the liver. Combined portal vein res...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-001-0110-8
更新日期:2001-10-01 00:00:00
abstract::The hypervascular nature of carcinoid tumors and their metastases allows a more aggressive role by the radiologist in diagnosis and interventional management. Double-contrast gastrointestinal studies still best define the primary neoplasms. Appendiceal tumors, the most frequent site of carcinoids, frequently escape ra...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s002689900023
更新日期:1996-02-01 00:00:00
abstract::The previous finding of an increased risk of premature death in a consecutive series of 896 patients operated on for primary hyperparathyroidism between 1953 and 1982 [1] raised the question of the role that surgery plays in relation to the risk of death. In the present study, undertaken to examine that issue, 3 facto...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF01658740
更新日期:1991-05-01 00:00:00
abstract:BACKGROUND:Primary hyperparathyroidism (HPT) is often caused by a benign parathyroid tumor, adenoma; less commonly by multiglandular parathyroid disease/hyperplasia; and rarely by parathyroid carcinoma. Patients with multiple tumors require wider exploration to avoid recurrence and have increased risk for hereditary di...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/s00268-009-0022-6
更新日期:2009-11-01 00:00:00
abstract:BACKGROUND:Although laparoscopic appendectomy (LA) is widely performed in many countries, LA for complicated appendicitis, which includes perforated or gangrenous appendicitis with or without localized or disseminated peritonitis, has not become a common practice yet. METHODS:We retrospectively analyzed the clinical r...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-008-9843-y
更新日期:2009-02-01 00:00:00
abstract::The surgical treatment of 30 cases of vascular thoracic outlet syndrome (TOS) in 25 patients is presented. Patients included 17 women and 8 men with average age of 26.1 years. The causes of compression were cervical rib ( n = 16), soft tissue anomalies ( n = 12), and scar tissue after clavicle fracture ( n = 2). Ten s...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-003-6808-z
更新日期:2003-05-01 00:00:00
abstract:BACKGROUND:The Severe Acute Respiratory Syndrome Coronavirus 2 pandemic has exposed surgeons to hazardous working conditions, imposing the need for personal protective equipment (PPE) use during surgery. The use of such equipment may affect their non-technical skills, augment fatigue, and affect performance. This study...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-020-05648-2
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Five billion people lack access to surgery. Accurate and complete data have been identified as essential to the global scale-up of perioperative care. This study retrospectively validates the Mbarara Surgical Services Quality Assurance Database (SQUAD), an electronic outcomes database at a Ugandan secondary ...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-020-05537-8
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Mutations in BRCA1 and BRCA2 confer an increased risk to breast and other cancers, but to date there have only been limited numbers of studies of BRCA1- and BRCA2-associated cancers among Asians. Malaysia is a multiracial country with three main races: Malays, Chinese, Indians. We determined whether tumor pa...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-009-0146-8
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND:Closure of loop ileostomy can be safely performed using sutures or staplers. The aim of the present study was to compare the cost effectiveness of three different techniques. METHODS:A total of 128 consecutive patients who underwent closure of loop ileostomy between January 2002 and December 2008 were analy...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-010-0787-7
更新日期:2010-12-01 00:00:00
abstract::This prospective study was instituted to assess whether the use of the on-pump method or the off-pump method affects changes in health-related quality of life (QOL) as evaluated a year after coronary artery bypass graft (CABG) surgery. Data including preoperative risk factors and postoperative morbidity up to discharg...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-004-7486-1
更新日期:2004-10-01 00:00:00
abstract::From the earliest description of dysphagia relieved by dilatation with a whalebone in 1674 we have witnessed the evolution of esophageal function testing from the conventional manometry to the high-resolution manometry (HRM) and esophageal topography pressure plotting that have led to the revised Chicago classificatio...
journal_title:World journal of surgery
pub_type: 历史文章,杂志文章
doi:10.1007/s00268-014-2939-7
更新日期:2015-07-01 00:00:00
abstract::In the Surgical Department of the University of Erlangen, 307 reconstructions in all areas of the arterial tree were performed with PTFE (GORE-TEX) grafts, of which 188 were performed in the femoral-popliteal and femoral-crural areas. Femoral-popliteal bypass grafts above the knee joint remained patent in 93% of the p...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/BF02393582
更新日期:1980-01-01 00:00:00
abstract:BACKGROUND:Concerns remain unresolved regarding the safety of unplanned conversion to open thoracotomy during video-assisted thoracic surgery (VATS) lobectomy. We analyzed both early and late outcomes after thoracotomy conversion from VATS. METHODS:From December 2003 to December 2008, a total of 738 VATS lobectomies w...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-010-0913-6
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:Small intestinal neuroendocrine neoplasms (SI-NEN) are rare, and only about 40% of patients are diagnosed without distant metastases. Aim of the study was to identify prognostic factors in patients with potentially curative resected locoregional SI-NEN. METHODS:Patients with curative resected locoregional S...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-020-05884-6
更新日期:2021-01-08 00:00:00
abstract:BACKGROUND:Scarce data are available on the characteristics of postoperative organ failure (POF) and mortality after gastrectomy. We aimed to describe the causes of organ failure and mortality related to gastrectomy for gastric cancer and to identify patients with POF who are at a risk of failure to rescue (FTR). METH...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-020-05382-9
更新日期:2020-05-01 00:00:00
abstract::Those benign breast disorders that are amenable to improvement by aesthetic surgery are discussed from the points of view of indications, techniques, and results. ...
journal_title:World journal of surgery
pub_type: 杂志文章,评审
doi:10.1007/BF01658430
更新日期:1989-11-01 00:00:00
abstract:BACKGROUND:The appropriate surgical approach for hepatocellular carcinoma (HCC) patients of Child-Pugh class B is unclear. The aim of this study was to clarify the prognostic factors after hepatectomy in Child-Pugh class B patients and to delineate the selection criteria for hepatectomy. METHODS:One hundred fifty pati...
journal_title:World journal of surgery
pub_type: 杂志文章
doi:10.1007/s00268-010-0929-y
更新日期:2011-04-01 00:00:00