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HLA DQ alpha typing of forensic specimens by amplification restriction fragment polymorphism (ARFP) analysis.

Abstract：

:The alleles present at the HLA DQ alpha locus may be typed by either allele specific oligonucleotide (ASO) probing or by restriction mapping, referred to here as amplification restriction fragment polymorphism (ARFP) analysis. ASO typing relies upon hybridization principles, whereas ARFP typing relies upon restriction site analysis. Dot-blot ASO typings which are of doubtful interpretation may be directly checked by ARFP analysis. Aliquots of the PCR products amplified using the commercial Amplitype HLA DQ alpha system are digested with suitable restriction endonucleases. Electrophoresis, blotting and detection of biotin-labelled restriction fragments provides a sensitive and robust typing method suited to forensic analysis.

journal_name

Forensic Sci Int

journal_title

Forensic science international

authors

Harrington CS,Dunaiski V,Williams KE,Fowler C

doi

10.1016/0379-0738(91)90215-5

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

147-57

issue

1

eissn

0379-0738

issn

1872-6283

pii

0379-0738(91)90215-5

journal_volume

51

pub_type

杂志文章

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