Species-specific oligonucleotides and multiplex PCR for forensic discrimination of two species of scallops, Placopecten magellanicus and Chlamys islandica.

Abstract:

:Characterization of DNA that remains in seafood products after skin, scales, and shells are removed is widely used in forensic species identification, however, ordinary methods may be prohibitively expensive or time-consuming if large sample series need to be discriminated. Forensic discrimination of two species of bivalves commercially harvested from the North Atlantic, sea scallops (Placopecten magellanicus) and Icelandic scallops (Chlamys islandica), was made by means of species-specific oligonucleotides (SSOs) in a multiplex polymerase chain reaction (PCR). The test is a simultaneous in vitro amplification of a portion of the mitochondrial Cytochrome Oxidase I locus with a PCR anchor primer for a sequence identical in both species, and two alternative SSOs that selectively amplify either a 619-bp in Placopecten or a 459-bp DNA fragment in Chlamys. Fragment size and thus species identity are determined directly by gel electrophoresis. In the forensic application, analysis of more than 900 scallops from a series of samples seized from two fishing vessels showed significantly variable proportions of the species from the closed and open fisheries (Placopecten versus Chlamys, respectively). The multiplex SSO test provides a direct means of forensic identification of large population sample series, without the necessity of secondary DNA sequencing, RFLP mapping, or fingerprinting, and can be adapted to other loci and species.

journal_name

Forensic Sci Int

authors

Marshall HD,Johnstone KA,Carr SM

doi

10.1016/j.forsciint.2006.05.043

subject

Has Abstract

pub_date

2007-03-22 00:00:00

pages

1-7

issue

1

eissn

0379-0738

issn

1872-6283

pii

S0379-0738(06)00343-4

journal_volume

167

pub_type

杂志文章
  • Greek land borders and migration fatalities - Humanitarian disaster described from the standpoint of Evros.

    abstract::The area of the regional unit of Evros in northern Greece is considered the main gateway to the East and marks the end of the European territory and the beginning of the Asian continent. The natural boundary of the Evros River has been the last major obstacle to overcome before entering the Schengen area for hundreds ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2019.109875

    authors: Pavlidis P,Karakasi MV

    更新日期:2019-09-01 00:00:00

  • Genetic identification of a war-evacuated child in search of his own identity for more than seventy years.

    abstract::V. M. E. was evacuated when he was a young boy in 1939. He left an aunt and cousins in Spain (G. E. family). He was adopted in Belgium by the D. family and thus his new name became V. D. He has been unable to remember his childhood before his adoption, a symptomatology compatible with amnesia for personal identity, pr...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2019.03.014

    authors: Puig P,Barceló A,Lahoz R,Niubó À,Jiménez J,Soler-López M,Donovan MJ,Navarro J,Camps J,Garcia-Caldés M,Etxeberria F,Miró R

    更新日期:2019-05-01 00:00:00

  • Quantitation of atenolol, metoprolol, and propranolol in postmortem human fluid and tissue specimens via LC/APCI-MS.

    abstract::Hypertension is a growing medical concern in the United States. With the number of Americans suffering from hypertension increasing, the use of antihypertensives such as beta-blockers is increasing as well. In fact, three beta-blockers - atenolol, metoprolol and propranolol - were among the 200 most prescribed medicat...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2005.01.001

    authors: Johnson RD,Lewis RJ

    更新日期:2006-01-27 00:00:00

  • Morphine levels in urine subsequent to poppy seed consumption.

    abstract::Urine morphine levels after the consumption of poppy seeds were measured in two separate trials. Maximum levels of approximately 18 micrograms/ml were found using RIA, EMIT-ST and GC methodologies. Positive immunoassay results were seen up to 60 h post-ingestion. Several different lots of seeds from various sources we...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(85)90173-2

    authors: Fritschi G,Prescott WR Jr

    更新日期:1985-02-01 00:00:00

  • Experimental study of the expansion dynamic of 9 mm Parabellum hollow point projectiles in ballistic gelatin.

    abstract::We study in this paper the expanding behaviour of hollow point 9 mm Parabellum projectiles (Hornady XTP(®) and Speer Gold Dot(®)). We defined a deformation rate that takes into account both the diameter increase and the length reduction. We plotted the behaviour of this parameter versus impact velocity (we refer to th...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2011.12.007

    authors: Bresson F,Ducouret J,Peyré J,Maréchal C,Delille R,Colard T,Demondion X

    更新日期:2012-06-10 00:00:00

  • Simultaneous analysis of 2Cs, 25-NBOHs, 25-NBOMes and LSD in seized exhibits using liquid chromatography-tandem mass spectrometry: A targeted approach.

    abstract::Blotter papers laced with lysergic acid diethylamide (LSD) have been abused traditionally for their hallucinogenic properties. In recent years, new psychedelic phenethylamines such as 2,5-dimethoxyphenethylamines (2C) and their N-benzylhydroxy (25-NBOH) and N-2-methoxybenzyl derivatives (25-NBOMe) have emerged in the ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2019.05.036

    authors: Chia XWS,Ong MC,Yeo YYC,Ho YJ,Binte Ahmad Nasir EI,Tan LJ,Chua PY,Yap TWA,Lim JLW

    更新日期:2019-08-01 00:00:00

  • Elemental analysis of bone: proton-induced X-ray emission testing in forensic cases.

    abstract::Proton-induced X-ray emission (PIXE) is a spectroscopic technique that provides the researcher with the elemental composition of a given target material. In this paper, we illustrate the utility of PIXE analysis in two forensic contexts: (1) case of cremation in which the nature of the remains is questioned and (2) ca...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(01)00614-4

    authors: Warren MW,Falsetti AB,Kravchenko II,Dunnam FE,Van Rinsvelt HA,Maples WR

    更新日期:2002-01-24 00:00:00

  • Y-chromosomal SNP haplotype diversity in forensic analysis.

    abstract::Many Y-chromosomal single nucleotide polymorphisms (SNPs) are now available. The haplogroups which they define are highly non-randomly distributed among populations, and could contribute much to population-of-origin prediction from DNA. If this potential is to be exploited in forensic analysis, high-throughput, parall...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(01)00385-1

    authors: Jobling MA

    更新日期:2001-05-15 00:00:00

  • The genetic male legacy from El Salvador.

    abstract::Allele frequencies and haplotype and haplogroup analysis have been performed for 16 Y-chromosome binary markers and 8 Y-chromosome STRs (DYS19, DYS385I and II, DYS389I and II, DYS390, DYS391, DYS392, DYS393). Data was obtained from a general sample of 93 unrelated individuals living in metropolitan areas from El Salva...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2006.07.005

    authors: Lovo-Gómez J,Blanco-Verea A,Lareu MV,Brión M,Carracedo A

    更新日期:2007-09-13 00:00:00

  • Early and late meningeal reaction to trauma after long-term brain death.

    abstract::We examined histologically the meninges adherent to traumatic lesions of a patient with brain death sustained for 101 days and observed both early and late reactions of wound healing at the same site of the dura mater. Some parts of the dura mater were thick and histological examinations revealed formation of new vess...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(92)90178-y

    authors: Ito Y,Kimura H

    更新日期:1992-10-01 00:00:00

  • Modeling microbial ethanol production by E. coli under aerobic/anaerobic conditions: applicability to real postmortem cases and to postmortem blood derived microbial cultures.

    abstract::The mathematical modeling of the microbial ethanol production under strict anaerobic experimental conditions for some bacterial species has been proposed by our research group as the first approximation to the quantification of the microbial ethanol production in cases where other alcohols were produced simultaneously...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2013.07.021

    authors: Boumba VA,Kourkoumelis N,Gousia P,Economou V,Papadopoulou C,Vougiouklakis T

    更新日期:2013-10-10 00:00:00

  • Cocaine and heroin in waste water plants: a 1-year study in the city of Florence, Italy.

    abstract::The diffusion and trends in use of each substance is a basic information in policy planning of strategies aiming at deterrence of drug abuse or in the organization of the fight against drug trafficking. The actual diffusion of illicit drugs in a population is hardly measurable, but, among the various measures availabl...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2009.04.018

    authors: Mari F,Politi L,Biggeri A,Accetta G,Trignano C,Di Padua M,Bertol E

    更新日期:2009-08-10 00:00:00

  • Sudden death due presumably to internal use of methamphetamine.

    abstract::A 26-year-old male was found naked and excited in the backyard of his neighbor's house. He was carried to a nearby hospital, and returned home with his family, but took a sudden turn for the worse and died. In a judicial autopsy, the ethanol concentration of blood was found to be 0.58 milligrams, and methamphetamine (...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(93)90209-s

    authors: Katsumata S,Sato K,Kashiwade H,Yamanami S,Zhou H,Yonemura I,Nakajima H,Hasekura H

    更新日期:1993-12-01 00:00:00

  • Isolation of the DNA minisatellite probe MZ 1.3 and its application to DNA 'fingerprinting' analysis.

    abstract::A minisatellite probe, MZ 1.3, detecting hypervariable fragment patterns was isolated from a human genomic library. A repetitive sequence of 27 bp length was identified which is contained in the probe approx. 40 times. The MZ 1.3 repeat shows variable homology of 53-73% to the repetitive sequence of the protein III ge...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(90)90252-t

    authors: Schacker U,Schneider PM,Holtkamp B,Bohnke E,Fimmers R,Sonneborn HH,Rittner C

    更新日期:1990-02-01 00:00:00

  • Sudden death: correlation histopathological and biochemical.

    abstract::The causes of sudden cardiac death are diverse and are function of age. In young people, coronary anomalies, hypertrophic cardiomyopathy are the most common findings at autopsies; in adults, coronary atherosclerosis and acquired forms of cardiomyopathy are frequent findings. In many of non-ischemia related cases autop...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2004.09.011

    authors: Martínez-Sánchez MC,Rodríguez-Vicente C

    更新日期:2004-12-02 00:00:00

  • Using virtual reality for forensic examinations of injuries.

    abstract::The ability to accurately determine injury dimensions is an essential property of forensic documentation. The current standard for injury documentation is photography using a scale to approximate the injury dimensions in the image. The technical qualities of the photograph, such as orthogonality, depth of the field an...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2018.11.006

    authors: Koller S,Ebert LC,Martinez RM,Sieberth T

    更新日期:2019-02-01 00:00:00

  • Histological examination of the temporal bone in medicolegal cases of asphyxia.

    abstract::Histological findings of the temporal bone in 23 autopsy cases of various asphyxial fatalities were studied. The temporal bones of 12 cases who died of tumors including mammary cancer, gastric cancer, myxoma of heart and craniopharyngioma, the bones of 3 cases of heart attack and the bones of 17 cases who died of vari...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(90)90243-r

    authors: Ito Y,Kimura H

    更新日期:1990-02-01 00:00:00

  • Fingermark detection on non-porous and semi-porous surfaces using NaYF4:Er,Yb up-converter particles.

    abstract::This article describes the first use of an anti-Stokes material, or up-converter, for the development of latent fingermarks on a range of non-porous surfaces. Anti-Stokes materials can absorb long-wavelength light and emit light at a shorter wavelength. This property is unusual in both natural and artificial materials...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2010.09.020

    authors: Ma R,Bullock E,Maynard P,Reedy B,Shimmon R,Lennard C,Roux C,McDonagh A

    更新日期:2011-04-15 00:00:00

  • Interaction between apnea, prone sleep position and gliosis in the brainstems of victims of SIDS.

    abstract::Among 27,000 infants studied prospectively to characterize their sleep-wake behavior, 38 infants died suddenly and unexpectedly under 6 months of age. Of these, 26 died from sudden infant death syndrome (SIDS), 5 from congenital cardiac abnormalities, 2 from infected pulmonary dysplasia, 2 from septic shock with multi...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/s0379-0738(02)00138-x

    authors: Sawaguchi T,Franco P,Kato I,Shimizu S,Kadhim H,Groswasser J,Sottiaux M,Togari H,Kobayashi M,Nishida H,Sawaguchi A,Kahn A

    更新日期:2002-09-14 00:00:00

  • Immunoassay evidence for fentanyl in hair of surgery patients.

    abstract::Head hair samples obtained from surgery patients who received fentanyl during anesthesia were analyzed by immunoassay for the presence of fentanyl. Thirteen hair samples were collected from patients following intravenous administration of 1-6 mg of fentanyl. Additional hair samples were collected following the adminis...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/0379-0738(93)90250-e

    authors: Wang WL,Cone EJ,Zacny J

    更新日期:1993-09-01 00:00:00

  • Serial postmortem thoracic radiographic findings in canine cadavers.

    abstract::Postmortem radiographic examinations of animals are often performed in judicial investigation to rule out gunshot and fractures due to cruelty. Literature describing postmortem changes seen on radiographs of animals is rarely available. Serial thoracic radiography of six recently euthanized dogs was performed in an in...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2009.03.031

    authors: Heng HG,Selvarajah GT,Lim HT,Ong JS,Lim J,Ooi JT

    更新日期:2009-07-01 00:00:00

  • The epidemiology of torture: a case series of 58 survivors of torture.

    abstract:UNLABELLED:INTRODUCTION/CONTEXT: Torture is widely practiced throughout the world and, yet, the ways by which torture is perpetrated, its regional similarities and differences, is not well understood. Our goal for this cases series was to elucidate the methods of torture practiced within different countries to both add...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2009.03.026

    authors: Sanders J,Schuman MW,Marbella AM

    更新日期:2009-08-10 00:00:00

  • Y-chromosomal STR haplotypes in Kalmyk population samples.

    abstract::Seventeen Y-chromosomal short tandem repeats (STRs), DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, GATA-H4, DYS448, DYS456, DYS458, DYS635 were typed in DNA samples from the Kalmyk population (n=99). The population is characterized by a high proportion of duplicated DYS19 ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2006.11.013

    authors: Roewer L,Krüger C,Willuweit S,Nagy M,Rodig H,Kokshunova L,Rothämel T,Kravchenko S,Jobling MA,Stoneking M,Nasidze I

    更新日期:2007-12-20 00:00:00

  • PIZZARO: Forensic analysis and restoration of image and video data.

    abstract::This paper introduces a set of methods for image and video forensic analysis. They were designed to help to assess image and video credibility and origin and to restore and increase image quality by diminishing unwanted blur, noise, and other possible artifacts. The motivation came from the best practices used in the ...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2016.04.027

    authors: Kamenicky J,Bartos M,Flusser J,Mahdian B,Kotera J,Novozamsky A,Saic S,Sroubek F,Sorel M,Zita A,Zitova B,Sima Z,Svarc P,Horinek J

    更新日期:2016-07-01 00:00:00

  • Evaluating the utility of hexapod species for calculating a confidence interval about a succession based postmortem interval estimate.

    abstract::Carrion insect succession patterns have long been used to estimate the postmortem interval (PMI) during a death investigation. However, no published carrion succession study included sufficient replication to calculate a confidence interval about a PMI estimate based on occurrence data. We exposed 53 pig carcasses (16...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2014.05.007

    authors: Perez AE,Haskell NH,Wells JD

    更新日期:2014-08-01 00:00:00

  • Inter- and intra-subject variability in ethanol pharmacokinetic parameters: effects of testing interval and dose.

    abstract::Calculation of a blood alcohol concentration (BAC) at the time of an offence by forward or back-extrapolation, using population average values for ethanol pharmacokinetic parameters or a single estimate of individual specific parameters, ignores the possibility of inter- and intra-subject variability. In order to esti...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2007.05.011

    authors: Yelland LN,Burns JP,Sims DN,Salter AB,White JM

    更新日期:2008-02-25 00:00:00

  • Development of a candidate method for forensic microbial genotyping using multiplex pyrosequencing combined with a universal biotinylated primer.

    abstract::Bacterial genotyping can be used for crime scene investigations and contribute to the attribution of biological attacks for microbial forensics. PyroMark ID Pyrosequencer as an accurate detection platform for single nucleotide polymorphisms (SNPs) has been applied to identify and resolve microorganisms involved in clo...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2014.11.004

    authors: Gu Y,Mao X,Zha L,Hou Y,Yun L

    更新日期:2015-01-01 00:00:00

  • The use of fingerprints available on the web in false identity documents: Analysis from a forensic intelligence perspective.

    abstract::Fingerprints present in false identity documents were found on the web. In some cases, laterally reversed (mirrored) images of a same fingerprint were observed in different documents. In the present work, 100 fingerprints images downloaded from the web, as well as their reversals obtained by image editing, were compar...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2016.02.041

    authors: Girelli CM

    更新日期:2016-05-01 00:00:00

  • Postmortem distribution of guaifenesin concentrations reveals a lack of potential for redistribution.

    abstract::Therapeutic (or non-toxic) postmortem guaifenesin blood and liver concentrations have not been previously described. Peripheral blood guaifenesin concentrations were compared to central blood and liver concentrations in eight medical examiner cases. Specimens were initially screened for alcohol and simple volatiles, d...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2014.10.029

    authors: McIntyre IM,Navarrete A,Mena O

    更新日期:2014-12-01 00:00:00

  • Identification of forensically important Chrysomya (Diptera: Calliphoridae) species using the second ribosomal internal transcribed spacer (ITS2).

    abstract::The identification of forensically important blowflies of the genus Chrysomya (Diptera: Calliphoridae) may be hampered by their close morphological similarities, especially as immatures. In contrast to most previous studies, the utility of a nuclear rather than mitochondrial genetic marker was investigated to solve th...

    journal_title:Forensic science international

    pub_type: 杂志文章

    doi:10.1016/j.forsciint.2008.01.009

    authors: Nelson LA,Wallman JF,Dowton M

    更新日期:2008-05-20 00:00:00