Microbial survival rates of Escherichia coli and Deinococcus radiodurans under low temperature, low pressure, and UV-Irradiation conditions, and their relevance to possible Martian life.

Abstract:

:Viability rates were determined for microbial populations of Escherichia coli and Deinococcus radiodurans under the environmental stresses of low temperature (-35 degrees C), low-pressure conditions (83.3 kPa), and ultraviolet (UV) irradiation (37 W/m(2)). During the stress tests the organisms were suspended in saltwater soil and freshwater soil media, at variable burial depths, and in seawater. Microbial populations of both organisms were most susceptible to dehydration stress associated with low-pressure conditions, and to UV irradiation. However, suspension in a liquid water medium and burial at larger depths (5 cm) improved survival rates markedly. Our results indicate that planetary surfaces that possess little to no atmosphere and have low water availability do not constitute a favorable environment for terrestrial microorganisms.

journal_name

Astrobiology

journal_title

Astrobiology

authors

Diaz B,Schulze-Makuch D

doi

10.1089/ast.2006.6.332

subject

Has Abstract

pub_date

2006-04-01 00:00:00

pages

332-47

issue

2

eissn

1531-1074

issn

1557-8070

journal_volume

6

pub_type

杂志文章
  • "Standoff Biofinder" for Fast, Noncontact, Nondestructive, Large-Area Detection of Biological Materials for Planetary Exploration.

    abstract:UNLABELLED:We developed a prototype instrument called the Standoff Biofinder, which can quickly locate biological material in a 500 cm(2) area from a 2 m standoff distance with a detection time of 0.1 s. All biogenic materials give strong fluorescence signals when excited with UV and visible lasers. In addition, the lu...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2015.1400

    authors: Misra AK,Acosta-Maeda TE,Sharma SK,McKay CP,Gasda PJ,Taylor GJ,Lucey PG,Flynn L,Abedin MN,Clegg SM,Wiens R

    更新日期:2016-09-01 00:00:00

  • Perspectives on Comets, Comet-like Asteroids, and Their Predisposition to Provide an Environment That Is Friendly to Life.

    abstract::In recent years, studies have shown that there are many similarities between comets and asteroids. In some cases, it cannot even be determined to which of these groups an object belongs. This is especially true for objects found beyond the main asteroid belt. Because of the lack of comet fragments, more progress has b...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2015.1354

    authors: Bosiek K,Hausmann M,Hildenbrand G

    更新日期:2016-04-01 00:00:00

  • Brines in seepage channels as eluants for subsurface relict biomolecules on Mars?

    abstract::Water, vital for life, not only maintains the integrity of structural and metabolic biomolecules, it also transports them in solution or colloidal suspension. Any flow of water through a dormant or fossilized microbial community elutes molecules that are potentially recognizable as biomarkers. We hypothesize that the ...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/153110701753198936

    authors: Wynn-Williams DD,Cabrol NA,Grin EA,Haberle RM,Stoker CR

    更新日期:2001-07-01 00:00:00

  • Experimental simulation of evaporation-driven silica sinter formation and microbial silicification in hot spring systems.

    abstract::Evaporation of silica-rich geothermal waters is one of the main abiotic drivers of the formation of silica sinters around hot springs. An important role in sinter structural development is also played by the indigenous microbial communities, which are fossilized and eventually encased in the silica matrix. The combina...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2012.0887

    authors: Orange F,Lalonde SV,Konhauser KO

    更新日期:2013-02-01 00:00:00

  • The NASA Astrobiology Roadmap.

    abstract::The NASA Astrobiology Roadmap provides guidance for research and technology development across the NASA enterprises that encompass the space, Earth, and biological sciences. The ongoing development of astrobiology roadmaps embodies the contributions of diverse scientists and technologists from government, universities...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/153110703769016299

    authors: Des Marais DJ,Allamandola LJ,Benner SA,Boss AP,Deamer D,Falkowski PG,Farmer JD,Hedges SB,Jakosky BM,Knoll AH,Liskowsky DR,Meadows VS,Meyer MA,Pilcher CB,Nealson KH,Spormann AM,Trent JD,Turner WW,Woolf NJ,Yorke HW

    更新日期:2003-07-01 00:00:00

  • Spectral signatures of photosynthesis. II. Coevolution with other stars and the atmosphere on extrasolar worlds.

    abstract::As photosynthesis on Earth produces the primary signatures of life that can be detected astronomically at the global scale, a strong focus of the search for extrasolar life will be photosynthesis, particularly photosynthesis that has evolved with a different parent star. We take previously simulated planetary atmosphe...

    journal_title:Astrobiology

    pub_type: 杂志文章,评审

    doi:10.1089/ast.2006.0108

    authors: Kiang NY,Segura A,Tinetti G,Govindjee,Blankenship RE,Cohen M,Siefert J,Crisp D,Meadows VS

    更新日期:2007-02-01 00:00:00

  • An exit cavity was crucial to the polymerase activity of the early ribosome.

    abstract::The emergence of an RNA entity capable of synthesizing peptides was a key prebiotic development. It is hypothesized that a precursor of the modern ribosomal exit tunnel was associated with this RNA entity (e.g., "protoribosome" or "bonding entity") from the earliest time and played an essential role. Various compounds...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2011.0692

    authors: Fox GE,Tran Q,Yonath A

    更新日期:2012-01-01 00:00:00

  • Impact of the Arizona NExSS Winter School on Astrobiology Knowledge and Attitudes.

    abstract::Astrobiology is an inherently interdisciplinary area of study, demanding communication across multiple fields: astronomy, geochemistry, planetary science, and so on. Successful communication requires that researchers be aware of the basic findings, open questions, and tools and techniques of allied fields and possess ...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2017.1710

    authors: Burnam-Fink M,Desch SJ,Scalice D,Davis H,Huff CJ,Apai D

    更新日期:2018-03-01 00:00:00

  • Extreme water loss and abiotic O2 buildup on planets throughout the habitable zones of M dwarfs.

    abstract::We show that terrestrial planets in the habitable zones of M dwarfs older than ∼1 Gyr could have been in runaway greenhouses for several hundred million years following their formation due to the star's extended pre-main sequence phase, provided they form with abundant surface water. Such prolonged runaway greenhouses...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2014.1231

    authors: Luger R,Barnes R

    更新日期:2015-02-01 00:00:00

  • Detection and Degradation of Adenosine Monophosphate in Perchlorate-Spiked Martian Regolith Analogue, by Deep-Ultraviolet Spectroscopy.

    abstract::The search for organic biosignatures on Mars will depend on finding material protected from the destructive ambient radiation. Solar ultraviolet can induce photochemical degradation of organic compounds, but certain clays have been shown to preserve organic material. We examine how the SHERLOC instrument on the upcomi...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2020.2362

    authors: Razzell Hollis J,Fornaro T,Rapin W,Wade J,Vicente-Retortillo Á,Steele A,Bhartia R,Beegle LW

    更新日期:2021-01-25 00:00:00

  • Identification and Characterization of Early Mission Phase Microorganisms Residing on the Mars Science Laboratory and Assessment of Their Potential to Survive Mars-like Conditions.

    abstract::Planetary protection is governed by the Outer Space Treaty and includes the practice of protecting planetary bodies from contamination by Earth life. Although studies are constantly expanding our knowledge about life in extreme environments, it is still unclear what the probability is for terrestrial organisms to surv...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2015.1417

    authors: Smith SA,Benardini JN 3rd,Anderl D,Ford M,Wear E,Schrader M,Schubert W,DeVeaux L,Paszczynski A,Childers SE

    更新日期:2017-03-01 00:00:00

  • The Case for Extant Life on Mars and Its Possible Detection by the Viking Labeled Release Experiment.

    abstract::The 1976 Viking Labeled Release (LR) experiment was positive for extant microbial life on the surface of Mars. Experiments on both Viking landers, 4000 miles apart, yielded similar, repeatable, positive responses. While the authors eventually concluded that the experiment detected martian life, this was and remains a ...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2015.1464

    authors: Levin GV,Straat PA

    更新日期:2016-10-01 00:00:00

  • Origin and formation of planetary systems.

    abstract::To estimate the occurrence of terrestrial exoplanets and maximize the chance of finding them, it is crucial to understand the formation of planetary systems in general and that of terrestrial planets in particular. We show that a reliable formation theory should not only explain the formation of the Solar System, with...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2009.0372

    authors: Alibert Y,Broeg C,Benz W,Wuchterl G,Grasset O,Sotin C,Eiroa C,Henning T,Herbst T,Kaltenegger L,Léger A,Liseau R,Lammer H,Beichman C,Danchi W,Fridlund M,Lunine J,Paresce F,Penny A,Quirrenbach A,Röttgering H,Selsi

    更新日期:2010-01-01 00:00:00

  • A sulfur-based survival strategy for putative phototrophic life in the venusian atmosphere.

    abstract::Several observations indicate that the cloud deck of the venusian atmosphere may provide a plausible refuge for microbial life. Having originated in a hot proto-ocean or been brought in by meteorites from Earth (or Mars), early life on Venus could have adapted to a dry, acidic atmospheric niche as the warming planet l...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/153110704773600203

    authors: Schulze-Makuch D,Grinspoon DH,Abbas O,Irwin LN,Bullock MA

    更新日期:2004-04-01 00:00:00

  • Analysis of competition between transformation pathways in the functioning of biotic abstract dual automata.

    abstract::Properties of avenues of transformation and their mutualism with forms of organization in dynamic systems are essential for understanding the evolution of prebiotic order. We have analyzed competition between two avenues of transformation in an A↔B system, using the simulation approach called BiADA (Biotic Abstract Du...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2012.0933

    authors: Popa R,Cimpoiasu VM

    更新日期:2013-05-01 00:00:00

  • Physicochemical Requirements Inferred for Chemical Self-Organization Hardly Support an Emergence of Life in the Deep Oceans of Icy Moons.

    abstract:UNLABELLED:An approach to the origin of life, focused on the property of entities capable of reproducing themselves far from equilibrium, has been developed recently. Independently, the possibility of the emergence of life in the hydrothermal systems possibly present in the deep oceans below the frozen crust of some of...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2015.1412

    authors: Pascal R

    更新日期:2016-05-01 00:00:00

  • Mitigation of the impact of terrestrial contamination on organic measurements from the Mars Science Laboratory.

    abstract::The objective of the 2009 Mars Science Laboratory (MSL), which is planned to follow the Mars Exploration Rovers and the Phoenix lander to the surface of Mars, is to explore and assess quantitatively a site on Mars as a potential habitat for present or past life. Specific goals include an assessment of the past or pres...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2007.0160

    authors: ten Kate IL,Canham JS,Conrad PG,Errigo T,Katz I,Mahaffy PR

    更新日期:2008-06-01 00:00:00

  • Modeling Repeated M Dwarf Flaring at an Earth-like Planet in the Habitable Zone: Atmospheric Effects for an Unmagnetized Planet.

    abstract::Understanding the impact of active M dwarf stars on the atmospheric equilibrium and surface conditions of a habitable zone Earth-like planet is key to assessing M dwarf planet habitability. Previous modeling of the impact of electromagnetic (EM) radiation and protons from a single large flare on an Earth-like atmosphe...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2017.1794

    authors: Tilley MA,Segura A,Meadows V,Hawley S,Davenport J

    更新日期:2019-01-01 00:00:00

  • A Simple Instrument Suite for Characterizing Habitability and Weathering: The Modern Aqueous Habitat Reconnaissance Suite (MAHRS).

    abstract::The shallow subsurface of Mars is extremely interesting as a possible microbial habitat because it becomes temporarily wet, it is shielded from radiation, and mixing by aeolian processes could provide the sources of energy and nutrients necessary for sustaining microbial life in it. The Modern Aqueous Habitat Reconnai...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2018.1945

    authors: Rennó NO,Backhus R,Cooper C,Flatico JM,Fischer E,Greer LC,Krasowski MJ,Kremic T,Martínez GM,Prokop NF,Sweeney D,Vicente-Retortillo A

    更新日期:2019-07-01 00:00:00

  • Terahertz circular dichroism spectroscopy: a potential approach to the in situ detection of life's metabolic and genetic machinery.

    abstract::We propose a terahertz (far-infrared) circular dichroism-based life-detection technology that may provide a universal and unequivocal spectroscopic signature of living systems regardless of their genesis. We argue that, irrespective of the specifics of their chemistry, all life forms will employ well-structured, chira...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/153110703322610609

    authors: Xu J,Ramian GJ,Galan JF,Savvidis PG,Scopatz AM,Birge RR,Allen SJ,Plaxco KW

    更新日期:2003-10-01 00:00:00

  • Enhanced Radiation Resistance of Methanosarcina soligelidi SMA-21, a New Methanogenic Archaeon Isolated from a Siberian Permafrost-Affected Soil in Direct Comparison to Methanosarcina barkeri.

    abstract:UNLABELLED:Permafrost-affected soils are characterized by a high abundance and diversity of methanogenic communities, which are considered suitable model organisms for potential life on Mars. Methanogens from Siberian permafrost have been proven to be highly resistant against divers stress conditions such as subzero te...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2015.1319

    authors: Morozova D,Moeller R,Rettberg P,Wagner D

    更新日期:2015-11-01 00:00:00

  • Challenges Analyzing Gypsum on Mars by Raman Spectroscopy.

    abstract::Raman spectroscopy can provide chemical information about organic and inorganic substances quickly and nondestructively with little to no sample preparation, thus making it an ideal instrument for Mars rover missions. The ESA ExoMars planetary mission scheduled for launch in 2018 will contain a miniaturized Raman spec...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2015.1334

    authors: Marshall CP,Olcott Marshall A

    更新日期:2015-09-01 00:00:00

  • Biogenesis and early life on Earth and Europa: favored by an alkaline ocean?

    abstract::Recent discoveries about Europa--the probable existence of a sizeable ocean below its ice crust; the detection of hydrated sodium carbonates, among other salts; and the calculation of a net loss of sodium from the subsurface--suggest the existence of an alkaline ocean. Alkaline oceans (nicknamed "soda oceans" in analo...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/153110702753621394

    authors: Kempe S,Kazmierczak J

    更新日期:2002-04-01 00:00:00

  • Assessing antibody microarrays for space missions: effect of long-term storage, gamma radiation, and temperature shifts on printed and fluorescently labeled antibodies.

    abstract::Antibody microarrays are becoming frequently used tools for analytical purposes. A key factor for optimal performance is the stability of the immobilized (capturing) antibodies as well as those that have been fluorescently labeled to achieve the immunological test (tracers). This is especially critical for long-distan...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2011.0647

    authors: de Diego-Castilla G,Cruz-Gil P,Mateo-Martí E,Fernández-Calvo P,Rivas LA,Parro V

    更新日期:2011-10-01 00:00:00

  • Sulfur-oxidizing chemolithotrophic proteobacteria dominate the microbiota in high arctic thermal springs on Svalbard.

    abstract::The thermal springs Trollosen and Fisosen, located on the High Arctic archipelago Svalbard, discharge saline groundwaters rich in hydrogen sulfide and ammonium through a thick layer of permafrost. Large amounts of biomass that consist of filamentous microorganisms containing sulfur granules, as analyzed with energy di...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2010.0551

    authors: Reigstad LJ,Jorgensen SL,Lauritzen SE,Schleper C,Urich T

    更新日期:2011-09-01 00:00:00

  • Subcritical water extractor for Mars analog soil analysis.

    abstract::Abstract Technologies that enable rapid and efficient extraction of biomarker compounds from various solid matrices are a critical requirement for the successful implementation of in situ chemical analysis of the martian regolith. Here, we describe a portable subcritical water extractor that mimics multiple organic so...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2007.0154

    authors: Amashukeli X,Grunthaner FJ,Patrick SB,Yung PT

    更新日期:2008-06-01 00:00:00

  • Phobos LIFE (Living Interplanetary Flight Experiment).

    abstract::The Planetary Society's Phobos Living Interplanetary Flight Experiment (Phobos LIFE) flew in the sample return capsule of the Russian Federal Space Agency's Phobos Grunt mission and was to have been a test of one aspect of the hypothesis that life can move between nearby planets within ejected rocks. Although the Phob...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2018.1904

    authors: Betts BH,Warmflash D,Fraze RE,Friedman L,Vorobyova E,Lilburn TG,Smith A,Rettberg P,Jönsson KI,Ciftcioglu N,Fox GE,Svitek T,Kirschvinck JL,Moeller R,Wassmann M,Berger T

    更新日期:2019-09-01 00:00:00

  • Field trial of a dual-wavelength fluorescent emission (L.I.F.E.) instrument and the Magma White rover during the MARS2013 Mars analog mission.

    abstract::Abstract We have developed a portable dual-wavelength laser fluorescence spectrometer as part of a multi-instrument optical probe to characterize mineral, organic, and microbial species in extreme environments. Operating at 405 and 532 nm, the instrument was originally designed for use by human explorers to produce a ...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2013.1081

    authors: Groemer G,Sattler B,Weisleitner K,Hunger L,Kohstall C,Frisch A,Józefowicz M,Meszyński S,Storrie-Lombardi M,Bothe C,Boyd A,Dinkelaker A,Dissertori M,Fasching D,Fischer M,Föger D,Foresta L,Frischauf N,Fritsch L,Fuchs

    更新日期:2014-05-01 00:00:00

  • The Mawrth Vallis region of Mars: A potential landing site for the Mars Science Laboratory (MSL) mission.

    abstract::The primary objective of NASA's Mars Science Laboratory (MSL) mission, which will launch in 2011, is to characterize the habitability of a site on Mars through detailed analyses of the composition and geological context of surface materials. Within the framework of established mission goals, we have evaluated the valu...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2010.0491

    authors: Michalski JR,Jean-PierreBibring,Poulet F,Loizeau D,Mangold N,Dobrea EN,Bishop JL,Wray JJ,McKeown NK,Parente M,Hauber E,Altieri F,Carrozzo FG,Niles PB

    更新日期:2010-09-01 00:00:00

  • Bacillus subtilis spore survival and expression of germination-induced bioluminescence after prolonged incubation under simulated Mars atmospheric pressure and composition: implications for planetary protection and lithopanspermia.

    abstract::Bacterial endospores in the genus Bacillus are considered good models for studying interplanetary transfer of microbes by natural or human processes. Although spore survival during transfer itself has been the subject of considerable study, the fate of spores in extraterrestrial environments has received less attentio...

    journal_title:Astrobiology

    pub_type: 杂志文章

    doi:10.1089/ast.2005.5.536

    authors: Nicholson WL,Schuerger AC

    更新日期:2005-08-01 00:00:00