Abstract:
:A single treatment of chick embryo fibroblasts with DNA recovered from chick embryo fibroblasts productively infected and transformed with four different strains of RSV, or productively infected with two different strains of RAV, resulted in virus production and cell transformation (in the case of RSV) two or three passages after treatment (8-25 days). The virus recovered from cultures was phenotypically identical to that produced by the donor cells. No virus production nor cell transformation resulted from treatment of control cultures with DNA digested with DNAse. Infectious RSV-DNA was recovered from purified donor cell nuclei and was associated with the precipitable fraction of DNA prepared according to the method of Hirt (1967). It also sedimented with cellular DNA in density gradients, and with high molecular weight DNA (2-4 times 10-7 daltons) in sucrose gradients, which suggests that it is associated and may be integrated with chromosomal DNA. In some experiments, DNA fractions of lower molecular weight (down to 6 times 10-6 daltons) were also infectious. DNA from virus-producing RSV-transformed cells also gave rise to virus and Rous cells in cultures of fibroblasts from gs- embryos. However, the amount of DNA required for successful infection varied widely between experiments, and no reproducible dose-effect relationship was observed. The frequency of DNA-treated cells which produced virus remained low, even when the assay cultures were pretreated with 5-bromodeoxyuridine.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Vigier P,Montagnier Ldoi
10.1002/ijc.2910150109keywords:
subject
Has Abstractpub_date
1975-01-15 00:00:00pages
67-77issue
1eissn
0020-7136issn
1097-0215journal_volume
15pub_type
杂志文章abstract::To obtain a comprehensive overview of chromosomal alterations that may underlie human papillomavirus (HPV)-mediated immortalization, 4 foreskin keratinocyte cell lines generated by transfection with either HPV 16 (cell lines FK16A and FK16B) or HPV 18 (FK18A and FK18B) were subjected to chromosomal analysis using comp...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980504)76:3<412::aid-ijc
更新日期:1998-05-04 00:00:00
abstract::Evidence of the association between chronic inflammation and the risk of colorectal cancer (CRC) and other obesity-related cancers (OBRC) remains inconsistent, possibly due to a paucity of studies examining repeated measures of inflammation. In the Health ABC prospective study of 2,490 adults aged 70-79 years at basel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29868
更新日期:2016-03-01 00:00:00
abstract::A "divergent pathway" model for the development of cutaneous melanoma has been proposed. The model hypothesizes that melanomas occurring in people with a low tendency to develop nevi will, on average, arise more commonly on habitually sun-exposed body sites such as the head and neck. In contrast, people with an inhere...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24011
更新日期:2009-02-15 00:00:00
abstract::Spleen cells from mice infected with Friend leukemia virus (FLV) inoculated by the intravenous route give rise to macroscopically visible colonies in the spleens of normal F1 histocompatible hybrid hosts. A study of H-2 antigens as generic markers for identification of strains of origin of cells constituting the splee...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910220418
更新日期:1978-10-15 00:00:00
abstract::Leukoplakia is an asymptomatic, potentially malignant change in the oral mucosa. Previous studies have reported that smoking and betel quid chewing are associated with increased risk of leukoplakia; few studies have reported on these associations in populations where betel quid does not contain tobacco. We conducted a...
journal_title:International journal of cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1002/ijc.23739
更新日期:2008-10-15 00:00:00
abstract::The aim of this study was to assess the diagnostic performance of breast imaging in the diagnosis of breast cancer in a non-screening population. In a consecutive set of patients referred for mammography in one year, the results of palpation and radiological breast imaging were scored on a 5-point grading scale and li...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.1235
更新日期:2001-05-15 00:00:00
abstract::A series of 722 lung carcinomas, surgically resected and typed some years ago according to the 1967 WHO classification, was independently reviewed by 2 observers in order to test the reproducibility of histopathological typing when using the criteria of the 1981 WHO classification. Typing was fully agreed upon in 87% ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910530205
更新日期:1993-01-21 00:00:00
abstract::In biliary tract cancer (BTC), tissue biopsies to guide treatment are rarely feasible, thus implementing liquid biopsy approaches to improve patient management represents a priority. So far, studies on circulating tumor cells (CTCs) in BTC are insufficient to promote their use in patient clinical management and are li...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32822
更新日期:2020-06-15 00:00:00
abstract::Although a screening program for gastric cancer, using barium X-ray examination, has been carried out widely in Japan for the past 3 decades, there is insufficient evidence to confirm its effectiveness in terms of reducing mortality. To evaluate the effectiveness of the screening, a population-based case-control study...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910600106
更新日期:1995-01-03 00:00:00
abstract::Non-specific cross-reacting antigens (NCA-95 = CD66b and NCA-50/90 = CD66c) are members of the CEA (carcinoembryonic antigen = CD66e) family. Analysis of mRNA levels of CD66c in colon tumors suggests that this antigen is strongly up-regulated compared to its normal counterpart and could, therefore, be of clinical inte...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910630308
更新日期:1995-11-03 00:00:00
abstract::Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity, representing 90% of all oral carcinomas and accounting for 3-5% of all malignancies. The WWOX gene (WW-domain containing oxidoreductase) is a candidate tumor suppressor gene located at 16q23.3-24.1, spanning the second most c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21446
更新日期:2006-03-01 00:00:00
abstract::Vitamin D has anticarcinogenic properties and might influence colorectal cancer (CRC) risk, but the epidemiological evidence is inconsistent. Many mechanisms of action for vitamin D have been proposed, with some of them initiating via its binding to the vitamin D receptor (VDR). Using a large Scottish case-control stu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23769
更新日期:2008-11-01 00:00:00
abstract::It has been proposed that oxidative stress develops in tumors, with important consequences for growth and progression. To investigate this hypothesis, we measured low m.w. thiols, disulfides, protein-mixed disulfides and a pool of major anti-oxidant enzymes in renal-cortex as well as renal-cell carcinoma (RCC) specime...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20010101)91:1<55::aid-ijc1006>3.
更新日期:2001-01-01 00:00:00
abstract::The radiosensitivity of three forms of response to injection of C. parvum in mice has been investigated. The increase in phagocytin index evoked by IP or IV injection of 0.7 mg C. parvum (but not that evoked by 1.4 mg) was reduced but not abolished in mice given 350-500 rad whole-body irradiation 4 days before C. parv...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170515
更新日期:1976-05-15 00:00:00
abstract::A goal of oncology is to predict chemosensitivity of tumors. This approach assumes that in a patient all tumor deposits are homogeneous. We have tested the heterogeneity between several samples of the same liver metastasis (LM; intrametastatic heterogeneity) or between multiple LM (intermetastatic heterogeneity) from ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25114
更新日期:2010-09-01 00:00:00
abstract::We investigated whether the concentration of fibronectin (FN) in the cerebrospinal fluid (CSF) could be used for identifying patients with subclinical blast-cell infiltration in the central nervous system (CNS) and an increased risk of CNS relapse later in the course of their leukemia. Our series comprised 36 children...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910430108
更新日期:1989-01-15 00:00:00
abstract::The tumor promoter 12-O-tetradecanoylphorbol 13-acetate (TPA) was found to increase the incidence of phenotypic alterations induced by the direct-acting carcinogen N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in human endometrial stromal cells. Following carcinogen treatment, changes in saturation density, gamma-glutam...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910320405
更新日期:1983-10-15 00:00:00
abstract::Inbred rats were hyper-immunised against a syngeneic transplanted mammary carcinoma, Sp4, which originally arose spontaneously in a breeding female. Spleen cells from Sp4-immune rats were fused with P3-NSI-Ag4 mouse myeloma cells, and supernatants from the hybrid progeny were screened for antibody reacting with Sp4 ce...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910260311
更新日期:1980-09-15 00:00:00
abstract::We treated 72 patients with metastatic renal-cell cancer according to 2 protocols consisting of two 5-week induction cycles of continuous i.v. high-dose interleukin-2 (IL-2), i.m. interferon-alpha (IFN alpha) and ex vivo IL-2-activated lymphocytes, followed for patients with stable disease (SD), partial response (PR) ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960117)65:2<152::AID-IJC
更新日期:1996-01-17 00:00:00
abstract::Although reproductive factors have been shown to be related to the composition of bile and functioning of the biliary system, their relationship with biliary tract cancer has not been studied in detail. Between 1984 and 1987 we conducted a case-control study of 75 women with cancer of the biliary tract and 252 control...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570203
更新日期:1994-04-15 00:00:00
abstract::The potential role of p16(INK4a) methylation in breast cancer is controversial whereas there are no data on fibroadenoma. To assess if inactivation of p16(INK4a) by promoter hypermethylation occurs in this hyperproliferative benign breast lesion or, on the contrary, it is strictly related to the carcinogenic process, ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.20771
更新日期:2005-04-10 00:00:00
abstract::Multiple drug resistance (MDR) is a problem that seriously reduces the efficacy of many chemotherapy agents. One mechanism for MDR is increased acidification of endocytic vesicles and increased cytosol pH, so weak base chemotherapeutic agents, including doxorubicin, are trapped in endocytic vesicles and exhibit a drug...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22098
更新日期:2006-12-01 00:00:00
abstract::A panel of eight conditionally immortal lines derived by infection of human breast epithelial cells with an amphotropic retrovirus transducing a ts mutant of SV40 large T-antigen was analyzed with respect to individual retroviral integration patterns. Each line contained multiple integration sites which were clonal an...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910570616
更新日期:1994-06-15 00:00:00
abstract::We performed an integrated array comparative genomic hybridization (aCGH) and expression microarray analysis of 8 normal gastric tissues and 38 primary tumors, including 25 intestinal and 13 diffuse gastric adenocarcinomas to identify genes whose expression is deregulated in association with copy number alteration. Ou...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23574
更新日期:2008-08-15 00:00:00
abstract::The effect of interferon on the long-term clinical outcome of patients with chronic hepatitis C remains unclear. This study included 594 patients with chronic hepatitis C who received interferon-alpha therapy (Interferon group) and 144 patients with chronic hepatitis C who did not receive interferon (Control group). T...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:
更新日期:2000-09-01 00:00:00
abstract::Current ovarian cancer maintenance therapy is limited by toxicity and no proven impact on overall survival. To study a maintenance strategy targeted at missense mutant p53, we hypothesized that the release of mutant p53 from mortalin inhibition by the SHetA2 drug combined with reactivation of mutant p53 with the PRIMA...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32830
更新日期:2020-08-15 00:00:00
abstract::In advanced stages of malignant melanoma (MM), metastases to the CNS are frequently observed. Few results are available on trophic factors and immunological features involved in the process of invasion and adhesion of circulating metastatic cells into the CNS. A direct comparison of remote metastases found in differen...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990924)83:1<135::aid-ijc
更新日期:1999-09-24 00:00:00
abstract::Human epidemiological studies suggest that 1,25(OH)2 D3 deficiency might increase cancer incidence, but no spontaneous tumors have been reported in mice lacking 1,25(OH)2 D3 or deficient in its receptor. In our study, we detected, for the first time, diverse types of spontaneous tumors in l,25(OH)2 D3 deficient mice m...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31317
更新日期:2018-07-15 00:00:00
abstract::BALB/c peritoneal plasmacytomas induced by a variety of agents are invariably associated with a c-myc translocation. In contrast, naturally arising bone marrow plasma cell tumors in C57BL/KaLwRij mice lack this translocation. This difference has led to the suggestion that these are 2 fundamentally different plasma cel...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19970904)72:5<892::aid-ijc
更新日期:1997-09-04 00:00:00
abstract::Increasing use of heavy ions for cancer therapy and concerns from exposure to heavy charged particles in space necessitate the study of the basic biological mechanisms associated with exposure to heavy ions. As the most critical damage induced by ionizing radiation is DNA double strand break (DSB), this review focuses...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.26445
更新日期:2012-03-01 00:00:00