Abstract:
:Mitochondrial dysfunction and pathology that contribute to a host of neurodegenerative diseases are deduced from changes in ultrastructure, routinely examined by a host of optical techniques. We adapted the technique of photon correlation spectroscopy (PCS) to evaluate calcium-induced structural alterations in isolated viable cortical and hippocampal mitochondria. In detecting calcium-induced reductions in light intensity, PCS was more sensitive than absorbance across varying calcium concentrations. Mitochondrial populations encompass a broad distribution of sizes, confirmed by ultrastructural profiles, both which remain unaffected by calcium exposure. Cortical and hippocampal populations show fractional calcium-induced reductions in light scatter compared to subsequent maximal alamethicin-induced reductions. Although reductions in light scatter (refractive index) have been interpreted as mitochondrial swelling, PCS quantification of the mean mitochondrial radius demonstrates that mitochondrial size is unaffected by calcium exposure, but not alamethicin. Likewise, the population distribution histograms remain stable with calcium exposure, but shift to larger radii after alamethicin exposure. Furthermore, hippocampal mitochondrial populations from a neurodegenerative model of traumatic brain injury, lateral fluid percussion, demonstrate greater calcium-induced reductions in scatter intensity, which are associated with an initial population of large mitochondria becoming smaller. The disparate responses to calcium and subsequent alamethicin of mitochondria at 3 and 24 h after injury attest to an acute disruption of membrane permeability in mitochondria from injured brain. PCS provides quantitative indices of refractive index and size in isolated mitochondrial populations, aiding the evaluation of mitochondria in degenerative diseases.
journal_name
Exp Neuroljournal_title
Experimental neurologyauthors
Lifshitz J,Janmey PA,McIntosh TKdoi
10.1016/j.expneurol.2005.10.002keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
318-29issue
2eissn
0014-4886issn
1090-2430pii
S0014-4886(05)00353-5journal_volume
197pub_type
杂志文章abstract::We evaluated the effects of allogeneic bone marrow stromal cell treatment of stroke on functional outcome, glial-axonal architecture, and immune reaction. Female Wistar rats were subjected to 2 h of middle cerebral artery occlusion. Rats were injected intravenously with PBS, male allogeneic ACI--or syngeneic Wistar--b...
journal_title:Experimental neurology
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abstract::Pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal peptide (VIP), and their receptors have been localized within the hippocampus but their physiological function on synaptic transmission is still unclear. We investigated the effects of PACAP and VIP on evoked excitatory postsynaptic curr...
journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2012.01.002
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abstract::It is clear that there are events which occur in response to neuromuscular activity that are essential to maintain normal muscle properties. Two factors though to influence muscle contractile properties are tension and electrical activity. To study these variables the hind limbs of 10 postpubertal female Sprague-Dawle...
journal_title:Experimental neurology
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abstract::Animal models treated with agricultural chemicals, such as rotenone, reproduce several degenerative features of human central nervous system (CNS) diseases. Glutamate is the most abundant excitatory amino acid transmitter in the mammalian central nervous system and its transmission is implicated in a variety of brain ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2007.09.023
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1995.1050
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1998.6862
更新日期:1998-09-01 00:00:00
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journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2005.03.004
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journal_title:Experimental neurology
pub_type: 社论
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2004.05.007
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abstract::This study uses the saphenous nerve crush model in Thy1-YFP mice and serial transcutaneous imaging to evaluate the rate of nerve regeneration under various FK-506 (tacrolimus) dosing regimens and in the presence of transgenic overexpression of glial cell line-derived neurotrophic factor (GDNF). Thy1-YFP transgenic mic...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2011.06.013
更新日期:2011-11-01 00:00:00
abstract::After spinal cord injury (SCI), the majority of individuals develop spasticity, a debilitating condition involving involuntary movements, co-contraction of antagonistic muscles, and hyperreflexia. By acting on GABAergic and Ca2+-dependent signaling, current anti-spastic medications lead to serious side effects, includ...
journal_title:Experimental neurology
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2015.04.020
更新日期:2015-09-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2014.05.019
更新日期:2014-11-01 00:00:00
abstract::Relatively few behavioral deficits are apparent in subjects with hereditary absence of the corpus callosum (CC). The anterior commissure (AC) has been suggested to provide an extracallosal route for the transfer of interhemispheric information in subjects with this congenital defect. Anterior commissure size, axon num...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1997.6564
更新日期:1997-08-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2017.03.019
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journal_title:Experimental neurology
pub_type: 杂志文章
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journal_title:Experimental neurology
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doi:10.1016/j.expneurol.2011.04.010
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journal_title:Experimental neurology
pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
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journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2020.113411
更新日期:2020-11-01 00:00:00
abstract::The GluN2 subunits that compose N-methyl-d-aspartate receptors (NMDARs) are attractive drug targets for therapeutic intervention in several diseases, in particular Parkinson's disease (PD). The precise roles and possible dysfunctions of NMDARs attributed to specific GluN2 subunits are however unresolved. Through the u...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2014.03.001
更新日期:2014-05-01 00:00:00
abstract::The cell death induced by hydroxyl radicals generated by Cu-phenanthroline and peroxynitrite generated by 3-morpholinosydnonimine hydrochloride (SIN-1) in rat primary cortical neuronal cultures was compared with the apoptotic death induced by staurosporine and the necrotic death induced by glutamate. Both SIN-1 and Cu...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.2000.7322
更新日期:2000-03-01 00:00:00
abstract::Mice lacking the gene encoding for substance P and neurokinin A, or the NK-1 receptor, exhibit alterations in behavior to various acute nociceptive stimuli. However, behavioral responses of NK-1 mutant animals have not been well characterized in models of chronic pain. We studied the behavioral responses of NK-1 knock...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1006/exnr.1999.7336
更新日期:2000-04-01 00:00:00
abstract::Local application of exogenous agents with neurotrophic properties enhances the regenerative capacity of injured neurons, especially following reconstructions of long nerve gaps and delayed nerve repairs. Recent advances in biomaterials and biomedical engineering have provided options for the sustained and controlled ...
journal_title:Experimental neurology
pub_type: 杂志文章,评审
doi:10.1016/j.expneurol.2018.08.014
更新日期:2019-09-01 00:00:00
abstract::An electron microscope study of 2- and 8-week-old normal and dystrophic chickens compared sarcomere lengths in relaxed and passively extended Patagialis (PAT) muscles. Sarcomeres were measured in dystrophic muscles only in fibers which exhibited no morphological signs of degeneration. Sarcomere lengths were not differ...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(88)90005-2
更新日期:1988-08-01 00:00:00
abstract::We investigated whether type 2 diabetes mellitus (T2DM), a risk factor of stroke, affects the level of scavenger receptor CD36 and the uptake of its ligand, oxidized LDL (oxLDL); and whether pioglitazone, a drug that enhances CD36, promotes oxLDL uptake. Compared to normoglycemic db/+ mice, adult db/db mice showed a p...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/j.expneurol.2020.113461
更新日期:2020-12-01 00:00:00
abstract::The precise role of hypothalamic norepinephrine (NE) in the control of vasopressin (AVP) release has remained unclear, due to reports of both inhibitory and excitatory effects of NE and only a few studies with direct hypothalamic manipulations. The present study utilized a chronically implanted swivel brain cannula to...
journal_title:Experimental neurology
pub_type: 杂志文章
doi:10.1016/0014-4886(90)90042-q
更新日期:1990-12-01 00:00:00