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A modified embryo cryopreservation method increases post-thaw survival with a concomitant increase in implantation.

Abstract：

OBJECTIVE:To achieve maximum post-thaw survival of frozen embryos. DESIGN:Historical controlled study. SETTING:Hospital-based fertility center. PATIENT(S):One hundred forty-five patients whose embryos were frozen and thawed according to the standard method, and 56 patients whose embryos were frozen and thawed according to a modified method. INTERVENTION(S):Modifications were made to the various steps of cryopreservation: freezing and thawing solutions, loading of embryos into the straws, and warming rates. MAIN OUTCOME MEASURE(S):Post-thaw survival, implantation, and pregnancy rates. RESULT(S):With the modified method, 138 (93%) of the 149 embryos thawed for 56 patients survived freezing, and 79.8% had all their blastomeres intact, which is almost double the result obtained (41.8%) for patients whose embryos were thawed with the standard method. The implantation and pregnancy rates were also significantly higher with the modified method compared with the standard method. CONCLUSION(S):Greater post-thaw embryo survival was achieved, with a concomitant increase in implantation and pregnancy rates, by modifying the various steps in the standard cryopreservation methodology. This has important implications in IVF practice.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Kattera S,Chen C

doi

10.1016/j.fertnstert.2005.04.054

keywords:

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

1498-504

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(05)02714-7

journal_volume

84

pub_type

杂志文章

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