Conditional recombination reveals distinct subsets of epithelial cells in trachea, bronchi, and alveoli.

abstract：:Retention kinetics for insoluble particles that deposit in the lung oftentimes resemble a multicomponent process during alveolar clearance, with each component appearing to follow simple first-order kinetics. Inasmuch as alveolar macrophages (AM) are thought to play an important role in particle removal from the lung,...

journal_title：American journal of respiratory cell and molecular biology

authors： Lehnert BE,Valdez YE,Tietjen GL

更新日期：1989-08-01 00:00:00

abstract：:Higher numbers of eosinophil/basophil colony-forming units (Eo/B CFU) are observed in blood of atopic individuals, and can be enhanced in atopic asthmatics by allergen-inhalation challenge. It is known that mature basophils and eosinophils synthesize cytokines relevant to allergic inflammation. To investigate the pote...

journal_title：American journal of respiratory cell and molecular biology

authors： Gauvreau GM,O'Byrne PM,Moqbel R,Velazquez J,Watson RM,Howie KJ,Denburg JA

更新日期：1998-07-01 00:00:00

abstract：:High mobility group box 1 (HMGB1) is an alarmin released from macrophages after infection or inflammation and is a biomarker of lung disease progression in patients with cystic fibrosis. We reported that 2-O, 3-O desulfated heparin (ODSH) inhibits the release of HMGB1 from murine macrophages triggered by neutrophil el...

journal_title：American journal of respiratory cell and molecular biology

authors： Zheng S,Kummarapurugu AB,Afosah DK,Sankaranarayanan NV,Boothello RS,Desai UR,Kennedy T,Voynow JA

更新日期：2017-01-01 00:00:00

abstract：:Mucin glycoproteins play a key role in the normal function of the airway epithelium. We examined the expression of mucin genes, MUC3, 4, 5AC, 5B, 6, 7, and 8 in human fetal tissues to establish the localization and age of onset of expression of each mucin gene during human development. We detected expression of MUC4, ...

journal_title：American journal of respiratory cell and molecular biology

authors： Reid CJ,Gould S,Harris A

更新日期：1997-11-01 00:00:00

abstract：:Protein kinase C (PKC) is known to regulate ryanodine receptor (RyR)-mediated local Ca(2+) signaling (Ca(2+) spark) in airway and vascular smooth muscle cells (SMCs), but its specific molecular mechanisms and functions still remain elusive. In this study, we reveal that, in airway SMCs, specific PKCepsilon peptide inh...

journal_title：American journal of respiratory cell and molecular biology

authors： Liu QH,Zheng YM,Korde AS,Li XQ,Ma J,Takeshima H,Wang YX

更新日期：2009-06-01 00:00:00

abstract：:Ventilator-induced lung injury (VILI) is a severe complication of mechanical ventilation that can lead to acute respiratory distress syndrome. VILI is characterized by damage to the epithelial barrier with subsequent pulmonary edema and profound hypoxia. Available lung-protective ventilator strategies offer only a mod...

journal_title：American journal of respiratory cell and molecular biology

authors： Dolinay T,Himes BE,Shumyatcher M,Lawrence GG,Margulies SS

更新日期：2017-08-01 00:00:00

abstract：:Clara-cell populations show a high degree of variation in susceptibility to injury by bioactivated cytotoxicants. Because glutathione (GSH) is critical for detoxification of electrophilic metabolites, heterogeneity in Clara cell GSH levels may lead to a wide range of cytotoxic responses. This study was designed to def...

journal_title：American journal of respiratory cell and molecular biology

authors： West JA,Chichester CH,Buckpitt AR,Tyler NK,Brennan P,Helton C,Plopper CG

更新日期：2000-07-01 00:00:00

abstract：:We examined the potential of several epithelial-derived factors to enhance neutrophil activation and survival. Neutrophils incubated in the presence of supernatants from nasal-derived primary epithelial cultures had significantly increased survival compared with neutrophils cultured in media alone. Of the cytokines re...

journal_title：American journal of respiratory cell and molecular biology

authors： Daffern PJ,Jagels MA,Hugli TE

更新日期：1999-08-01 00:00:00

abstract：:In rat models of Gram-negative pneumonia, pulmonary emigration of neutrophils (polymorphonuclear leukocytes [PMNs]) is blocked when rats are made endotoxemic by an intravenous administration of endotoxin (lipopolysaccharide [LPS]). To test whether dysfunctional PMN migratory responses in the endotoxemic rat are specif...

journal_title：American journal of respiratory cell and molecular biology

authors： Wagner JG,Driscoll KE,Roth RA

更新日期：1999-04-01 00:00:00

abstract：:Gene therapy requires the presence of a robust and yet small promoter to drive high-level expression of desired proteins. In comparative analysis, we investigated the promoter strength of the CTP:phosphocholine cytidylyltransferase promoter (CCT alpha) with other commonly used promoters, which were all cloned into a s...

journal_title：American journal of respiratory cell and molecular biology

authors： Zhou J,You Y,Zabner J,Ryan AJ,Mallampalli RK

更新日期：2004-01-01 00:00:00

abstract：:Elevated concentrations of CO2 (hypercapnia) lead to alveolar epithelial dysfunction by promoting Na,K-ATPase endocytosis. In the present report, we investigated whether the CO2/HCO3(-) activated soluble adenylyl cyclase (sAC) regulates this process. We found that hypercapnia increased the production of cyclic adenosi...

journal_title：American journal of respiratory cell and molecular biology

authors： Lecuona E,Sun H,Chen J,Trejo HE,Baker MA,Sznajder JI

更新日期：2013-05-01 00:00:00

abstract：:Airway infections or irritant exposures during early postnatal periods may contribute to the onset of childhood asthma. The purpose of this study was to examine critical periods of postnatal airway development during which ozone (O(3)) exposure leads to heightened neural responses. Rats were exposed to O(3) (2 ppm) or...

journal_title：American journal of respiratory cell and molecular biology

authors： Hunter DD,Wu Z,Dey RD

更新日期：2010-12-01 00:00:00

abstract：:Sox2, a transcription factor critical for the maintenance of embryonic stem cells and induction of pluripotent stem cells, is expressed exclusively in the conducting airway epithelium of the lung, where it is required for differentiation of nonciliated, goblet, and ciliated cells. To determine the role of Sox2 in resp...

journal_title：American journal of respiratory cell and molecular biology

authors： Tompkins DH,Besnard V,Lange AW,Keiser AR,Wert SE,Bruno MD,Whitsett JA

更新日期：2011-07-01 00:00:00

abstract：:Mechanical ventilation with high tidal volumes has been associated with pulmonary alveolar flooding. Understanding the mechanisms underlying cyclic stretch-induced increases in alveolar epithelial permeability may be important in designing preventive measures for acute lung injury. In this work, we assessed whether cy...

journal_title：American journal of respiratory cell and molecular biology

authors： Davidovich N,DiPaolo BC,Lawrence GG,Chhour P,Yehya N,Margulies SS

更新日期：2013-07-01 00:00:00

abstract：:Laminins are heterotrimeric proteins that are secreted by the alveolar epithelium into the basement membrane, and their expression is altered in extracellular matrices from patients with pulmonary fibrosis. In a small number of patients with pulmonary fibrosis, we found that the normal basement membrane distribution o...

journal_title：American journal of respiratory cell and molecular biology

authors： Morales-Nebreda LI,Rogel MR,Eisenberg JL,Hamill KJ,Soberanes S,Nigdelioglu R,Chi M,Cho T,Radigan KA,Ridge KM,Misharin AV,Woychek A,Hopkinson S,Perlman H,Mutlu GM,Pardo A,Selman M,Jones JC,Budinger GR

更新日期：2015-04-01 00:00:00

abstract：:To better understand asthma pathogenesis, we characterized airway responsiveness, lung inflammation, and mediator production of alveolar macrophages (AM) after allergen sensitization and challenge in two strains of rats showing different susceptibilities in developing airway allergic reactions. Airway responsiveness t...

journal_title：American journal of respiratory cell and molecular biology

authors： Careau E,Sirois J,Bissonnette EY

更新日期：2002-05-01 00:00:00

abstract：:Enhanced airway smooth muscle (ASM) contractility contributes to increased resistance to airflow in diseases such as bronchitis and asthma that occur in passive smokers exposed to secondhand smoke. Little information exists on the cellular mechanisms underlying such airway hyperreactivity. Sputum samples of patients w...

journal_title：American journal of respiratory cell and molecular biology

authors： Sathish V,Vanoosten SK,Miller BS,Aravamudan B,Thompson MA,Pabelick CM,Vassallo R,Prakash YS

更新日期：2013-04-01 00:00:00

abstract：:Mucus hypersecretion is characteristic of chronic airway diseases. However, regulatory mechanisms are poorly understood. Human airway epithelial cells grown on permeable supports at the air-liquid interface (ALI) develop a mucociliary morphology resembling that found in vivo. Such cultures provide a model for studying...

journal_title：American journal of respiratory cell and molecular biology

authors： Bernacki SH,Nelson AL,Abdullah L,Sheehan JK,Harris A,Davis CW,Randell SH

更新日期：1999-04-01 00:00:00

abstract：:Pneumocystis is an important fungal pathogen that causes life-threatening pneumonia in patients with AIDS and malignancy. Lung fungal pathogens are recognized by C-type lectin receptors (CLRs), which bind specific ligands and stimulate innate immune responses. The CLR Dectin-1 was previously shown to mediate immune re...

journal_title：American journal of respiratory cell and molecular biology

authors： Kottom TJ,Hebrink DM,Jenson PE,Marsolek PL,Wüthrich M,Wang H,Klein B,Yamasaki S,Limper AH

更新日期：2018-02-01 00:00:00

abstract：:Clara cell secretory protein (CCSP), or CC10, is an inhibitor of secretory phospholipase A2 which may be produced by phagocytic cells and by a variety of other cells in the airway. Tumor necrosis factor-alpha (TNF-alpha) is capable of activating phospholipases and inducing the expression of a variety of genes in the a...

journal_title：American journal of respiratory cell and molecular biology

authors： Yao XL,Levine SJ,Cowan MJ,Logun C,Shelhamer JH

更新日期：1998-10-01 00:00:00

abstract：:Tumor necrosis factor-alpha (TNF-alpha) is thought to be important in the development of pulmonary fibrosis. However, surfactant protein-C/TNF-alpha transgenic mice do not spontaneously develop pulmonary fibrosis but instead develop alveolar enlargement and loss of elastic recoil. We hypothesized that overexpression o...

journal_title：American journal of respiratory cell and molecular biology

authors： Fujita M,Shannon JM,Morikawa O,Gauldie J,Hara N,Mason RJ

更新日期：2003-12-01 00:00:00

abstract：:Polymorphonuclear leukocytes (PMN) and eosinophils (Eos) are important cellular participants in a variety of acute and chronic inflammatory reactions in the airway. Histologic evidence has implicated direct interactions between these two subsets of leukocytes and airway epithelial cells during inflammation. A comprehe...

journal_title：American journal of respiratory cell and molecular biology

authors： Jagels MA,Daffern PJ,Zuraw BL,Hugli TE

更新日期：1999-09-01 00:00:00

abstract：:Exposure to particulate air pollution is associated with increased cardiopulmonary morbidity and mortality, although the pathogenic mechanisms are poorly understood. We previously demonstrated that particulate matter (PM) exposure triggers massive oxidative stress in vascular endothelial cells (ECs), resulting in the ...

journal_title：American journal of respiratory cell and molecular biology

authors： Wang T,Wang L,Zaidi SR,Sammani S,Siegler J,Moreno-Vinasco L,Mathew B,Natarajan V,Garcia JG

更新日期：2012-10-01 00:00:00

abstract：:Severe deterioration of surfactant function is noted under conditions of plasma protein leakage into the alveolar space; moreover, fibrinogen has previously been reported to possess strong surfactant inhibitory capacity. Dissolution of alveolar deposits of fibrinogen and fibrin (e.g., hyaline membranes) requires enzym...

journal_title：American journal of respiratory cell and molecular biology

authors： Seeger W,Grube C,Günther A

更新日期：1993-09-01 00:00:00

abstract：:Although neutrophils play critical roles in innate immunity, in excess these cells cause severe tissue damage. Thus, neutrophil activation must be tightly regulated to prevent indiscriminant damage. Previously, we reported that mice lacking matrix metalloproteinase (MMP) 7 are protected from lung injury owing to marke...

journal_title：American journal of respiratory cell and molecular biology

authors： Gill SE,Nadler ST,Li Q,Frevert CW,Park PW,Chen P,Parks WC

更新日期：2016-08-01 00:00:00

abstract：:Recent evidence suggests that tyrosine kinases play an important role in signal transduction mechanisms utilized by a range of different agonists in many cell types. We have investigated the effects of four different inhibitors of tyrosine kinases on IgE-dependent histamine release from human lung mast cells and basop...

journal_title：American journal of respiratory cell and molecular biology

authors： Lavens SE,Peachell PT,Warner JA

更新日期：1992-12-01 00:00:00

abstract：:Idiopathic pneumonia syndrome (IPS) is a significant clinical problem encountered among patients treated with bone marrow transplantation (BMT). IPS is identified as an inflammatory lung disease characterized by diffuse interstitial pneumonitis and alveolitis leading to interstitial fibrosis in the absence of an ident...

journal_title：American journal of respiratory cell and molecular biology

authors： Shankar G,Scott Bryson J,Darrell Jennings C,Kaplan AM,Cohen DA

更新日期：1999-06-01 00:00:00

abstract：:The gaseous molecule carbon monoxide (CO) is elevated in the breath of individuals with asthma. The physiologic function of CO in asthma is poorly understood. Here we demonstrate that CO (250 ppm) markedly inhibits human airway smooth muscle cell (HASMC) proliferation, arresting cells at the G0/G1 phase. This CO-induc...

journal_title：American journal of respiratory cell and molecular biology

authors： Song R,Mahidhara RS,Liu F,Ning W,Otterbein LE,Choi AM

更新日期：2002-11-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) is necessary for normal postnatal lung development and may underlie the structural lung damage that follows hyperoxic exposure. To determine the individual roles of VEGF receptors (VEGFR) 2 and 1 on postnatal lung growth, neonatal mice were treated with neutralizing antibodies...

journal_title：American journal of respiratory cell and molecular biology

authors： McGrath-Morrow SA,Cho C,Cho C,Zhen L,Hicklin DJ,Tuder RM

更新日期：2005-05-01 00:00:00

abstract：:Primary pulmonary hypertension is characterized by increased pulmonary vascular resistance and smooth muscle proliferation. Stable analogs are increasingly being used to treat this disease, although no data exists comparing their effects on proliferation. We therefore investigated the antiproliferative activity of sev...

journal_title：American journal of respiratory cell and molecular biology

authors： Clapp LH,Finney P,Turcato S,Tran S,Rubin LJ,Tinker A

更新日期：2002-02-01 00:00:00