Destabilization of apoprotein is insufficient to explain Cu,Zn-superoxide dismutase-linked ALS pathogenesis.

abstract：:The yeast flora found in the major substrates of Drosophila mojavensis and in larval guts was studied both qualitatively and quantitatively. Quantitative studies show that, in four of the five substrates tested, the larvae did not contain a random sample of the yeasts present in the substrates. One widely distributed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fogleman JC,Starmer WT,Heed WB

更新日期：1981-07-01 00:00:00

abstract：:The exact role of the endocannabinoid system (ECS) during spermatogenesis has not been clarified. We used purified germ cell fractions representative of all phases of spermatogenesis and primary cultures of spermatogonia. This approach allowed the precise quantification of the cannabinoid receptor ligands, anandamide ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grimaldi P,Orlando P,Di Siena S,Lolicato F,Petrosino S,Bisogno T,Geremia R,De Petrocellis L,Di Marzo V

更新日期：2009-07-07 00:00:00

abstract：:A somatic hybrid of ASL-1 leukemia cells [H-2a, thymus leukemia (TL)1,2,3, Thy-1b] and LM(TK)-cells [H-2-k, TL(-), Thy-1 (-), thymidine kinase deficient] was formed with the aid of inactivated Sendai virus. The selection of hybrid cell clones was facilitated by the inability of ASL-1 cells to grow in vitro and of LM(T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang W,Cohen EP

更新日期：1975-05-01 00:00:00

abstract：:Archaea of the phylum Thaumarchaeota are among the most abundant prokaryotes on Earth and are widely distributed in marine, terrestrial, and geothermal environments. All studied Thaumarchaeota couple the oxidation of ammonia at extremely low concentrations with carbon fixation. As the predominant nitrifiers in the oce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Könneke M,Schubert DM,Brown PC,Hügler M,Standfest S,Schwander T,Schada von Borzyskowski L,Erb TJ,Stahl DA,Berg IA

更新日期：2014-06-03 00:00:00

abstract：:Apurinic/apyrimidinic (AP) sites are common DNA lesions that arise from spontaneous depurination or by base excision repair (BER) of modified bases. A biotin-containing aldehyde-reactive probe (ARP) [Kubo, K., Ide, H., Wallace, S. S. & Kow, Y. W. (1992) Biochemistry 31, 3703-3708] is used to measure AP sites in living...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Atamna H,Cheung I,Ames BN

更新日期：2000-01-18 00:00:00

abstract：:Primary transcripts made in vitro on bacteriophage T4 DNA by RNA polymerase isolated from normal or T4-infected Escherichia coli were compared by gel electrophoresis. Bacteriophage-modified RNA polymerase fails to initiate transcription at certain promoters recognized by unmodified enzyme. In the T4tRNA gene region, o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldfarb A

更新日期：1981-06-01 00:00:00

abstract：:We have examined the affinity of two recently synthesized flavin analogs for the isoalloxazine binding site of riboflavin-binding protein (RBP). The results showed that pyrimidopteridines could bind to RBP (Kd 160-250 microM). This suggested that, at the FMN or FAD level, these analogs might also bind to other apoflav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wessiak A,Schopfer LM,Yuan LC,Bruice TC,Massey V

更新日期：1984-07-01 00:00:00

abstract：:Learning the properties of an image associated with human gaze placement is important both for understanding how biological systems explore the environment and for computer vision applications. There is a large literature on quantitative eye movement models that seeks to predict fixations from images (sometimes termed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kümmerer M,Wallis TS,Bethge M

更新日期：2015-12-29 00:00:00

abstract：:The membrane glycoproteins specified by herpes simplex virus are synthesized concurrently with structural viral proteins and accumulate in the cytoplasm and in the membranes lining it. Analyses of free and membranebound polyribosomes, the cytoplasmic pool of soluble proteins, and purified smooth membranes showed that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spear PG,Roizman B

更新日期：1970-07-01 00:00:00

abstract：:Although ghrelin has been demonstrated to stimulate energy intake and storage through a central mechanism, its effect on hepatic lipid metabolism remains largely uncharacterized. Ghrelin receptor antagonism or gene deletion significantly decreased obesity-associated hepatic steatosis by suppression of de novo lipogene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Xu G,Qin Y,Zhang C,Tang H,Yin Y,Xiang X,Li Y,Zhao J,Mulholland M,Zhang W

更新日期：2014-09-09 00:00:00

abstract：:Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common blood disorder, presenting multiple symptoms, including hemolytic anemia. It affects 400 million people worldwide, with more than 160 single mutations reported in G6PD. The most severe mutations (about 70) are classified as class I, leading to more...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horikoshi N,Hwang S,Gati C,Matsui T,Castillo-Orellana C,Raub AG,Garcia AA,Jabbarpour F,Batyuk A,Broweleit J,Xiang X,Chiang A,Broweleit R,Vöhringer-Martinez E,Mochly-Rosen D,Wakatsuki S

更新日期：2021-01-26 00:00:00

abstract：:In this study we sought to better understand the role of the glycoprotein quality control machinery in the assembly of MHC class I molecules with high-affinity peptides. The lectin-like chaperone calreticulin (CRT) and the thiol oxidoreductase ERp57 participate in the final step of this process as part of the peptide-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wearsch PA,Peaper DR,Cresswell P

更新日期：2011-03-22 00:00:00

abstract：:We evaluated the formation and removal of (+-)-3 alpha,4 beta-dihydroxy-1 alpha,2 alpha-epoxy-1,2,3,4- tetrahydrobenzo[c]phenanthrene (BcPHDE)-DNA adducts in two Chinese hamster ovary (CHO) cell lines. One line of repair-proficient cells (MK42) carries a stable 150-fold amplification of the dihydrofolate reductase (DH...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carothers AM,Zhen W,Mucha J,Zhang YJ,Santella RM,Grunberger D,Bohr VA

更新日期：1992-12-15 00:00:00

abstract：:Among the tRNA population of the archaeal parasite Nanoarchaeum equitans are five species assembled from separate 5' and 3' tRNA halves and four species derived from tRNA precursors containing introns. In both groups an intervening sequence element must be removed during tRNA maturation. A bulge-helix-bulge (BHB) moti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Randau L,Calvin K,Hall M,Yuan J,Podar M,Li H,Söll D

更新日期：2005-12-13 00:00:00

abstract：:The RuvB protein is induced in Escherichia coli as part of the SOS response to DNA damage. It is required for genetic recombination and the postreplication repair of DNA. In vitro, the RuvB protein promotes the branch migration of Holliday junctions and has a DNA helicase activity in reactions that require ATP hydroly...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stasiak A,Tsaneva IR,West SC,Benson CJ,Yu X,Egelman EH

更新日期：1994-08-02 00:00:00

abstract：:Global climate change is expected to have broad ecological consequences for species and communities. Attempts to forecast these consequences usually assume that changes in air or water temperature will translate into equivalent changes in a species' organismal body temperature. This simple change is unlikely because a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gilman SE,Wethey DS,Helmuth B

更新日期：2006-06-20 00:00:00

abstract：:The mechanism of preferential transmission (i.e., maternal inheritance) of cytoplasmic genes was investigated with chloroplast DNA of Chlamydomonas as a model system. The behavior of nuclear and chloroplast DNAs were compared in the sexual cycle; DNAs from male and female parents were distinguished by labeling with (1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sager R,Lane D

更新日期：1972-09-01 00:00:00

abstract：:Effects of thyrotropin-releasing hormone (TRH; thyroliberin) on membrane electrical properties were studied in the clonal rat anterior pituitary cell (GH3) by continuous recording of the intracellular potential. Application of TRH, which stimulates the release of prolactin and growth hormone from the GH3 cell, elicite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozawa S,Kimura N

更新日期：1979-11-01 00:00:00

abstract：:MDM2 and MDMX are homologous proteins that bind to p53 and regulate its activity. Both contain three folded domains and ~70% intrinsically disordered regions. Previous detailed structural and biophysical studies have concentrated on the isolated folded domains. The N-terminal domains of both exhibit high affinity for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bista M,Petrovich M,Fersht AR

更新日期：2013-10-29 00:00:00

abstract：:Estrogen signaling occurs through at least two distinct molecular pathways: (i) direct binding of liganded estrogen receptors (ERs) to estrogen-responsive DNA elements (EREs) (the "ER/ERE pathway") and (ii) indirect recruitment of liganded ERs to activating protein-1 (AP-1)-responsive DNA elements via heterodimers of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheung E,Acevedo ML,Cole PA,Kraus WL

更新日期：2005-01-18 00:00:00

abstract：:The superparamagnetic behavior of very small particles of metallic iron (ca 1.5 nm), with about half of their atoms at the surface, is changed reversibly by adsorption and desorption of hydrogen below the superparamagnetic transition temperature. The change after adsorption implies a lowering of the anisotropy energy ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boudart M,Dumesic JA,Topsøe H

更新日期：1977-03-01 00:00:00

abstract：:Exosomes have been proposed as vehicles for microRNA (miRNA) -based intercellular communication and a source of miRNA biomarkers in bodily fluids. Although exosome preparations contain miRNAs, a quantitative analysis of their abundance and stoichiometry is lacking. In the course of studying cancer-associated extracell...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chevillet JR,Kang Q,Ruf IK,Briggs HA,Vojtech LN,Hughes SM,Cheng HH,Arroyo JD,Meredith EK,Gallichotte EN,Pogosova-Agadjanyan EL,Morrissey C,Stirewalt DL,Hladik F,Yu EY,Higano CS,Tewari M

更新日期：2014-10-14 00:00:00

abstract：:Biochemical evidence is presented for the disintegration of the lens fiber plasma membrane in human cataracts. The intrinsic membrane proteins are found in both the water-soluble and water-insoluble nonmembrane fractions of the cataract lens but not in the normal tissue. Furthermore, in contrast to the normal lens, no...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garner MH,Roy D,Rosenfeld L,Garner WH,Spector A

更新日期：1981-03-01 00:00:00

abstract：:Rotaviruses are the single most common cause of fatal and severe childhood diarrheal illness worldwide (>125 million cases annually). Rotavirus shares structural and functional features with many viruses, such as the presence of segmented double-stranded RNA genomes selectively and tightly packed with a conserved numb...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Periz J,Celma C,Jing B,Pinkney JN,Roy P,Kapanidis AN

更新日期：2013-07-16 00:00:00

abstract：:The structural analysis of the Fab' fragment of human myeloma immunoglobulin IgGl(lambda) New has been extended to a nominal resolution of 2.0 A. Each of the structural subunits corresponding to the variable and to the constant homology regions of the light and heavy chains contains two irregular beta-sheets which are...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poljak RJ,Amzel LM,Chen BL,Phizackerley RP,Saul F

更新日期：1974-09-01 00:00:00

abstract：:Despite advances in resolution accompanying the development of high-field superconducting magnets, biomolecular applications of NMR require multiple dimensions in order to resolve individual resonances, and the achievable resolution is typically limited by practical constraints on measuring time. In addition to the ne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maciejewski MW,Fenwick M,Schuyler AD,Stern AS,Gorbatyuk V,Hoch JC

更新日期：2011-10-04 00:00:00

abstract：:DNA polymerases from Xenopus laevis oocytes, unfertilized eggs, and kidney cells grown in culture have been characterized. The same three major DNA polymerase activities are present in all cell types examined. We attempt to relate the characteristics of amphibian enzymes to those of mammalian polymerases. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tatò F,Gandini DA,Tocchini-Valentini GP

更新日期：1974-09-01 00:00:00

abstract：:Dispersed cell cultures were established from 7- to 9-day postnatal mouse cerebellum. The fibrin slice method was used to obtain a localization of plasminogen activator production to specific cells. Fibrinolytically active cells were small (5- to 8-micrometer diameter), round, and occurred singly or in aggregates. Fib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krystosek A,Seeds NW

更新日期：1981-12-01 00:00:00

abstract：:Our laboratory has previously developed cell lines derived from mouse NIH 3T3 fibroblasts and C127 mammary tumor cells that stably express mouse mammary tumor virus (MMTV) long terminal repeat fusion genes in bovine papillomavirus-based episomes. Glucocorticoid hormone strongly activates transcription from episomes an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bresnick EH,John S,Berard DS,LeFebvre P,Hager GL

更新日期：1990-05-01 00:00:00

abstract：:To find the native conformation (fold), proteins sample a subspace that is typically hundreds of orders of magnitude smaller than their full conformational space. Whether such fast folding is intrinsic or the result of natural selection, and what is the longest foldable protein, are open questions. Here, we derive the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin MM,Zewail AH

更新日期：2012-06-19 00:00:00