Abstract:
:In this study, we investigated the sequence variation and different transcriptional activities of the upstream regulatory region (URR) in HPV 16 E7 variants in cervical cancer tissue from Korean women. Using PCR-directed sequencing, the presence of sequence variations in URRs were analyzed and the sites of sequence variation were matched with the known transcriptional factor binding site (TFBS) in 26 HPV 16 E7 variants, 21 cases with A647G (KE7-1, high oncogenic potential) and 5 cases with T732C (KE7-2, low oncogenic potential). In addition, we determined the transcriptional activity of URR in a HPV 16 prototype, and in 4 cases of HPV 16 E7 variants, by using the functional chloramphenicol acetyl transferase (CAT) assay. The 26 HPV 16 E7 variants showed more than 11 sites of sequence variation in the URR. Ten sites of sequence variation were located in the known TFBS and the distribution of sequence variations in the URR showed clear differences between KE7-1 and KE7-2. The sequence variations T7781C and C7786T were matched with YY1 binding sites, G7193T and C7689A were matched with TEF1 binding sites, and C7394T and C7395T were matched with GRE binding sites. The other sequence variations, which were matched with the TFBS, were A7485C, G7489A, T7743G and G7842A. The URR activity of KE7-1 was significantly lower than that of the HPV 16 prototype, whilst KE7-2 was similar. Taken together with the results of the transcriptional activities of KE7-1 and KE7-2, our results suggest that the functional activity and sequence variations of HPV 16 URR may not be related to the oncogenic potential of HPV 16 E7 variants.
journal_name
Oncol Repjournal_title
Oncology reportsauthors
Kim YB,Song YS,Jeon YT,Park JS,Um SJ,Kim JW,Park NH,Kang SB,Lee HPkeywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
459-64issue
2eissn
1021-335Xissn
1791-2431journal_volume
14pub_type
杂志文章相关文献
ONCOLOGY REPORTS文献大全abstract::There is increasing evidence of an angiogenic response of irradiated tumors resulting in decreased radiation sensitivity. However, little is known about the contribution of tumor vascular endothelial growth factor (VEGF) or basic fibroblast growth factor (bFGF)-release induced by irradiation to the individual level of...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00
abstract::Prostate cancer (PCa) growth initially depends on circulating androgens. Gonadotropin-releasing hormone (GnRH) agonists are currently used for the treatment of PCa. However, after an initial responsiveness to hormonal deprivation, PCa progresses and metastasizes. Recently, also GnRH antagonists have been used for clin...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.15.2.393
更新日期:2006-02-01 00:00:00
abstract::Non-small cell lung cancer (NSCLC) patients with epithelial growth factor receptor (EGFR) mutations and bone metastases are often concurrently administered tyrosine kinase inhibitors (TKIs) and bisphosphonates. Yet, the effects and mechanisms of these agents are unclear. In the present study, we aimed to ascertain whe...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4741
更新日期:2016-06-01 00:00:00
abstract::The nitrostyrene scaffold was previously identified as a lead target structure for the development of effective compounds targeting Burkitt's lymphoma. The present study aimed to develop these compounds further in haematological malignancies, including chronic lymphocytic leukaemia (CLL). Cellular viability, flow cyto...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7068
更新日期:2019-05-01 00:00:00
abstract::Implicated in several physiologic processes, including tumour-host, some transglutaminases (TGase) have been noted to play a regulatory role in the extracellular matrix in cell adhesion and migration of cancer cells. This study sought to determine the level of expression of TGases and their possible clinical significa...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::MicroRNAs (miRNAs or miRs) contribute to the development of various malignant neoplasms, including glioblastoma multiforme (GBM). The present study aimed to explore the pathogenesis of GBM and to identify latent therapeutic agents for patients with GBM, based on an in silico analysis. Gene chips that provide miRNA exp...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7022
更新日期:2019-04-01 00:00:00
abstract::Neuroblastoma is an aggressive childhood disease. Even with intensive conventional treatments, the long term survival rate for children with neuroblastoma remains less than 40%, highlighting the importance of finding new therapies. Bcl-2 family proteins play crucial roles in survival, proliferation and chemotherapeuti...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3228
更新日期:2014-08-01 00:00:00
abstract::Lapatinib, an inhibitor of human epidermal growth factor receptor 2 (HER2) phosphorylation, has been reported to inhibit several types of tumors such as HER2-overexpressing breast cancer. However, the effect of lapatinib on the malignant phenotype of human osteosarcoma (OS) cells and the potential molecular mechanisms...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2825
更新日期:2014-01-01 00:00:00
abstract::Rad52 encodes a protein which is required for recombinational repair of double-strand breaks. It is also associated with breast cancer predisposition genes BRCA1 and BRCA2. Mutations in the genes Rad51 or Rad52 result in severe defects in genetic recombination and the repair of double-strand DNA breaks. In order to ex...
journal_title:Oncology reports
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::Previous studies have suggested that certain genetic polymorphisms, specifically the Xeroderma pigmentosum group D (XPD) gene codon 751 and the X-ray repair cross-complementing group 1 (XRCC1) gene codon 399 polymorphisms, were associated with an increased risk of lung cancer, and, in some studies, with a greater risk...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000854
更新日期:2010-07-01 00:00:00
abstract::Human Pinx1 protein, associated with shelterin proteins, is widely revealed as a haploinsufficient tumor suppressor. Growing evidence has manifested the deregulation of PinX1 in distinct cancers. Nonetheless, the loss status of PinX1 and its diagnostic, prognostic and clinicopathological significance in Basal-like bre...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2017.5696
更新日期:2017-07-01 00:00:00
abstract::Exhausted cluster of differentiation (CD)8+ T cells lose immunological activity due to mitochondrial dysfunction caused by peroxisome proliferator‑activated receptor γ coactivator 1α (PGC‑1α) inactivation, resulting in a poor prognosis in patients with cancer. As hydrogen gas was recently reported to activate PGC‑1α, ...
journal_title:Oncology reports
pub_type: 杂志文章,随机对照试验
doi:10.3892/or.2018.6841
更新日期:2019-01-01 00:00:00
abstract::Bmi-1, a member of the polycomb family, it is involved in self renewal of stem cells and functions as an oncogene in many malignant human cancer types. Recent studies have demonstrated that Bmi-1 is a predictive factor for poor patient prognosis. However, the underlying mechanisms o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1615
更新日期:2012-04-01 00:00:00
abstract::The present study aimed to explore the relationship between the efficacy of epidermal growth factor receptor tyrosine kinase inhibitors (EGFR‑TKIs) and the expression of the cancer stem cell (CSC)‑related markers. Specimens of 72 cases of lung adenocarcinoma with significantly different therapeutic effects of the firs...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7454
更新日期:2020-02-01 00:00:00
abstract::Large‑scale projects, such as The Cancer Genome Atlas (TCGA), Human Epigenome Project (HEP) and Human Epigenome Atlas (HEA), provide an insight into DNA methylation and histone modification markers. Changes in the epigenome significantly contribute to the initiation and progression of cancer. The goal of the present s...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7159
更新日期:2019-07-01 00:00:00
abstract::The targeting of receptor activator of nuclear factor-κB ligand (RANKL) is being increasingly investigated as a potential therapeutic strategy in several types of cancers. However, the exact function and mechanism of RANKL in human endometrial cancer (EC), particularly in progesterone-resistant and aggressive EC, rema...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2014.3651
更新日期:2015-02-01 00:00:00
abstract::Using a series of potential biomarkers relevant to mechanisms of protein synthesis, we observed that estrogen receptor (ER)-positive breast tumor cells exist in two distinct yet interconvertible phenotypic states (of roughly equal proportion) which differ in the degree of differentiation and use of IRES-mediated trans...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6332
更新日期:2018-06-01 00:00:00
abstract::Podoplanin is a 38 kDa mucin-type transmembrane glycoprotein that was first identified in rat glomerular epithelial cells (podocytes). It is expressed in normal lymphatic endothelium, but is absent from vascular endothelial cells. D2-40 is a commercially available mouse monoclonal antibody which binds to an epitope on...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1141
更新日期:2011-03-01 00:00:00
abstract::Formation of cancer stem cells (CSCs) and increased cells proliferation are involved in tumorigenesis, tumour recurrence and therapy resistance and microRNA is essential for the development of the biological traits of CSCs and the increased cells proliferation. Studying molecular mechanism of tumorigenesis, tumour rec...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5149
更新日期:2016-12-01 00:00:00
abstract::The peroxisome proliferator-activated receptor-γ (PPAR-γ) coactivator-1β (PGC-1β) is a well-established regulator of mitochondrial biogenesis. However, the underlying mechanism of PGC-1β action remains elusive. This study reveals that knockdown of endogenous PGC-1β by short-hairpin RNA (shRNA) leads to a decrease in t...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2013.2628
更新日期:2013-10-01 00:00:00
abstract::Arginine is a critical amino acid in specific cancer types including hepatocellular carcinoma (HCC) and melanoma. Novel molecular mechanisms and therapeutic targets in arginine metabolism-mediated cancer formation await further identification. Our laboratory has previously demonstrated that arginine metabolic enzyme a...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.5334
更新日期:2017-02-01 00:00:00
abstract::Head and neck squamous cell carcinoma (HNSCC) is the sixth most commonly diagnosed cancer worldwide. It has poor clinical outcome due to intrinsic or acquired drug resistance. Deregulation of both apoptosis and autophagy contributes to chemotherapy resistance and disease progression. A new member of the inhibitors of ...
journal_title:Oncology reports
pub_type: 杂志文章,评审
doi:10.3892/or.2019.7082
更新日期:2019-05-01 00:00:00
abstract::Gallbladder cancer (GBC) is a lethal aggressive malignant neoplasm of the biliary tract. Potential prognostic markers and therapeutic targets for this disease are urgently required. Cancer‑associated fibroblasts (CAFs) play a key role in tumorigenesis and the development of cancer. Nicotinamide adenine dinucleotide ph...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2019.7249
更新日期:2019-10-01 00:00:00
abstract::P5, which is a member of the protein disulfide isomerase family, possesses isomerase and chaperone activity in vitro; however, the physiological functions of this enzyme in cells remain unclear. To understand the important roles of P5 in cancer cells, the present study examined its expression on the surface of normal ...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2018.6868
更新日期:2019-02-01 00:00:00
abstract::9-Oxo-10, 11-dehydroageraphorone (euptox A), a cadenine sesquiterpene, is the main toxin from Eupatorium adenophorum. The aim of the present study was to examine the induction and mechanism of HeLa cell apoptosis by euptox A. The apoptosis‑inducing effect of the euptox A on HeLa cells was examined by MTT assay. The un...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3778
更新日期:2015-04-01 00:00:00
abstract::The clinical significance of NK-like T CD3+/CD16+CD56+ cells in chronic lymphocytic leukemia (CLL) is still a subject of controversy. There are few previous descriptions that this cell population can be qualitatively or quantitatively deficient in CLL patients. In the present study we investigated the clinical value o...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or_00000924
更新日期:2010-09-01 00:00:00
abstract::Thyroid cancer (TC) is the most frequent endocrine malignancy, accounting however for only 1-2% of all human cancers, and the best-established risk factor for TC is radiation exposure, particularly during childhood. Since the BER pathway seems to play an important role in the repair of DNA damage induced by IR and oth...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2012.1975
更新日期:2012-11-01 00:00:00
abstract::CD147, a multifunction glycoprotein of the immuno-globulin family, is associated with many types of tumors, and the overexpression of CD147 can enhance malignancy. However, the role of CD147 in cervical cancer remains unclear. In the present study, cervical cancer cells were found to express much more CD147 protein an...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2011.1345
更新日期:2011-09-01 00:00:00
abstract::High mobility group box-B1 (HMGB1), an autophagy activator, is crucial in tumorigenesis. However, its extracellular role and signaling in gastric cancer remain unclear. Samples were collected from gastric cancer patients and healthy controls. Immunohistochemistry and immunocytochemistry were used to determine the loca...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2015.3782
更新日期:2015-04-01 00:00:00
abstract::The high affinity nerve growth factor (NGF) NGF receptor, p75NTR, is a member of the tumor necrosis factor (TNF) receptor superfamily that shares a conserved intracellular death domain capable of inducing apoptosis and suppressing growth in prostate epithelial cells. Expression of this receptor is lost as prostate can...
journal_title:Oncology reports
pub_type: 杂志文章
doi:10.3892/or.2016.4832
更新日期:2016-07-01 00:00:00