Comparison of angiogenic activities after urethral reconstruction using free grafts in rabbits.

Abstract:

OBJECTIVE:To determine the most suitable type of graft-free penile skin grafts or mucosal grafts from bladder or buccal regions - for urethral reconstruction in an animal model, as evaluated on the basis of angiogenic activity. METHODS:Twenty-two male White New Zealand rabbits were randomly divided into four groups. In the control group (group O, n=4) a simple urethrotomy and closure was performed, whereas a ventral urethral defect was created in groups A, B, and C and then bridged using the following onlay patches: free penile skin (group A, n=6), buccal mucosal graft (group B, n=6), and bladder mucosal graft (group C, n=6). On the 21st postoperative day, the animals were sacrificed and the retrieved implants were subjected to macroscopic and microscopic analysis. The angiogenic activity was assessed with immunohistochemistry, using the anti-CD31 MoAb and the phosphatase antialkaline phosphatase procedure. The native vascularity of penile skin as well as buccal and bladder mucosa was assessed in rabbits from group O (n=3). Statistical analysis was performed using the one-way ANOVA. RESULTS:The angiogenesis in a magnification of x200 in groups O, A, B, and C was 34.1+/-4.1 (mean+/-SD), 61.7+/-6.4, 94.3+/-6.4, and 91.5+/-7.2 vessels per optical field, respectively. There were, statistically significant differences (p<0.001) between groups A and B and between groups A and C, but not (p>0.05) between groups B and C. The native vascularity of penile skin, buccal mucosa and bladder mucosa was 23.3+/-3.0, 24.6+/-3.7 and 17.0+/-2.6 vessels per optical field, respectively. CONCLUSION:The viability of mucosal grafts from bladder or buccal regions is better than that of a free penile graft because of higher angiogenic activity. Although the mucosal grafts showed the same angiogenic activity, the buccal mucosa graft is preferable because of its easier harvesting.

journal_name

Eur Urol

journal_title

European urology

authors

Gardikis S,Giatromanolaki A,Ypsilantis P,Botaitis S,Perente S,Kambouri A,Efstathiou E,Antypas S,Polychronidis A,Touloupidis S,Sivridis E,Simopoulos C

doi

10.1016/j.eururo.2004.10.014

keywords:

subject

Has Abstract

pub_date

2005-03-01 00:00:00

pages

417-21

issue

3

eissn

0302-2838

issn

1873-7560

pii

S0302-2838(04)00521-4

journal_volume

47

pub_type

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