Guanyl nucleotides modulate binding to steroid receptors in neuronal membranes.

abstract：:Bone mineralization is an essential step during the embryonic development of vertebrates, and bone serves vital functions in human physiology. To systematically identify unique gene functions essential for osteogenesis, we performed a forward genetic screen in zebrafish and isolated a mutant, no bone (nob), that does ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huitema LF,Apschner A,Logister I,Spoorendonk KM,Bussmann J,Hammond CL,Schulte-Merker S

更新日期：2012-12-26 00:00:00

abstract：:Translesion synthesis at replication-blocking lesions requires the induction of proteins that are controlled by the SOS system in Escherichia coli. Of the proteins identified so far, UmuD', UmuC, and RecA* were shown to facilitate replication across UV-light-induced lesions, yielding both error-free and mutagenic tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fuchs RP,Napolitano RL

更新日期：1998-10-27 00:00:00

abstract：:The detection of biological molecules and their interactions is a significant component of modern biomedical research. In current biosensor technologies, simultaneous detection is limited to a small number of analytes by the spectral overlap of their signals. We have developed an NMR-based xenon biosensor that capital...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spence MM,Rubin SM,Dimitrov IE,Ruiz EJ,Wemmer DE,Pines A,Yao SQ,Tian F,Schultz PG

更新日期：2001-09-11 00:00:00

abstract：:We have identified two chromosomal genes of Escherichia coli K12 that are required for the expression of conjugative plasmid functions in the presence of normal plasmid DNA. Hfr cells with mutations in both of these genes are resistant to donor-specific bacteriophage and defective as conjugal donors. These characteris...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McEwen J,Silverman P

更新日期：1980-01-01 00:00:00

abstract：:We have identified a gene, rig (rat insulinoma gene), that is activated in chemically induced rat insulinomas but not in normal pancreatic islets or in regenerating islets. In the present study, we have found that the insulinoma gene was activated in a BK virus-induced hamster insulinoma cell line and in a spontaneous...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inoue C,Shiga K,Takasawa S,Kitagawa M,Yamamoto H,Okamoto H

更新日期：1987-10-01 00:00:00

abstract：:Upon cytosolic viral DNA stimulation, cGMP-AMP synthase (cGAS) catalyzes synthesis of 2'3'cGMP-AMP (cGAMP), which binds to the adaptor protein MITA (mediator of IRF3 activation, also called STING, stimulator of IFN genes) and induces innate antiviral response. How the activity of MITA/STING is regulated to avoid exces...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xia T,Yi XM,Wu X,Shang J,Shu HB

更新日期：2019-10-01 00:00:00

abstract：:We present a comparative study of calorimetrically derived thermodynamic profiles for the binding of a series of drugs with selected DNA host duplexes. We use these data to demonstrate that comparisons between complete thermodynamic profiles (delta G zero, delta H zero, delta S zero, delta Cp) are required before drug...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breslauer KJ,Remeta DP,Chou WY,Ferrante R,Curry J,Zaunczkowski D,Snyder JG,Marky LA

更新日期：1987-12-01 00:00:00

abstract：:H2O2, in concentrations achieved in the proximity of stimulated leukocytes, induces injury and lysis of target cells. This may be an important aspect of inflammatory injury of tissues. Cell lysis in two target cells, the murine macrophage-like tumor cell line P388D1 and human peripheral lymphocytes, was found to be as...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schraufstatter IU,Hyslop PA,Hinshaw DB,Spragg RG,Sklar LA,Cochrane CG

更新日期：1986-07-01 00:00:00

abstract：:The amplitude of the CO(2) seasonal cycle at the Mauna Loa Observatory (MLO) increased from the early 1970s to the early 1990s but decreased thereafter despite continued warming over northern continents. Because of its location relative to the large-scale atmospheric circulation, the MLO receives mainly Eurasian air m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buermann W,Lintner BR,Koven CD,Angert A,Pinzon JE,Tucker CJ,Fung IY

更新日期：2007-03-13 00:00:00

abstract：:Branching morphogenesis in the mammary gland is achieved by the migration of epithelial cells through a microenvironment consisting of stromal cells and extracellular matrix (ECM). Here we show that galectin-1 (Gal-1), an endogenous lectin that recognizes glycans bearing N-acetyllactosamine (LacNAc) epitopes, induces ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat R,Belardi B,Mori H,Kuo P,Tam A,Hines WC,Le QT,Bertozzi CR,Bissell MJ

更新日期：2016-08-16 00:00:00

abstract：:We determined that a pigeon cytochrome c-derived peptide, p43-58, possesses two anchor residues, 46 and 54, for binding with the I-Ab molecule that are compatible to the position 1 (P1) and position 9 (P9) of the core region in the major histocompatibility complex (MHC) class II binding peptides, respectively. In the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Itoh Y,Kajino K,Ogasawara K,Takahashi A,Namba K,Negishi I,Matsuki N,Iwabuchi K,Kakinuma M,Good RA,Onoé K

更新日期：1997-10-28 00:00:00

abstract：:Contact of axons with target territories results in the formation of synapses, specific junctional complexes that may represent a final stage of neuronal maturation. Synaptosomal-associated protein 25 (SNAP-25) is a component of particular nerve terminals recently identified in rodent brain. To evaluate the structure ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Catsicas S,Larhammar D,Blomqvist A,Sanna PP,Milner RJ,Wilson MC

更新日期：1991-02-01 00:00:00

abstract：:Interleukin 6 (IL-6) is a cytokine that acts on various cell types. Here we show that IL-6 mRNA is produced in vivo in two self-limiting physiologic angiogenic processes: (i) the formation of the vascular system accompanying development of ovarian follicles and (ii) the formation of a capillary network in the maternal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Motro B,Itin A,Sachs L,Keshet E

更新日期：1990-04-01 00:00:00

abstract：:In many of their compounds the transition metals have covalence 9, forming nine bonds with use of nine hybrid spd bond orbitals. A set of maximum-valence single-bond radii is formulated for use in these compounds. These radii are in reasonably good agreement with observed bond lengths. Quadruple bonds between two tran...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pauling L

更新日期：1975-10-01 00:00:00

abstract：:Genetically engineered mice with targeted disruption of the neuronal nitric oxide synthase (nNOS) gene established the inhibitory role of nitric oxide (NO) in male impulsive aggressive behavior. This was later confirmed by using selective nNOS inhibitors in male wild-type mice. The molecular mechanisms accounting for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chiavegatto S,Dawson VL,Mamounas LA,Koliatsos VE,Dawson TM,Nelson RJ

更新日期：2001-01-30 00:00:00

abstract：:The high-affinity (K(m) = 3 x 10(-8) M) transport system for histidine in Salmonella typhimurium has been resolved into three components: J, K, and P. J, which is a histidine-binding protein released by osmotic shock, is specified by the hisJ gene: hisJ mutants lack the binding protein and are defective in histidine t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ames GF,Lever J

更新日期：1970-08-01 00:00:00

abstract：:Ler, a homolog of H-NS in enteropathogenic Escherichia coli (EPEC), plays a critical role in the expression of virulence genes encoded by the pathogenic island, locus of enterocyte effacement (LEE). Although Ler acts as an antisilencer of multiple LEE operons by alleviating H-NS-mediated silencing, it represses its ow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat A,Shin M,Jeong JH,Kim HJ,Lim HJ,Rhee JH,Paik SY,Takeyasu K,Tobe T,Yen H,Lee G,Choy HE

更新日期：2014-06-24 00:00:00

abstract：:Transplantation of pancreatic islets for treating type 1 diabetes is restricted to patients with critical metabolic lability resulting from the need for immunosuppression and the shortage of donor organs. To overcome these barriers, we developed a strategy to macroencapsulate islets from different sources that allow t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ludwig B,Ludwig S,Steffen A,Knauf Y,Zimerman B,Heinke S,Lehmann S,Schubert U,Schmid J,Bleyer M,Schönmann U,Colton CK,Bonifacio E,Solimena M,Reichel A,Schally AV,Rotem A,Barkai U,Grinberg-Rashi H,Kaup FJ,Avni Y,J

更新日期：2017-10-31 00:00:00

abstract：:The amphiphilic fluorescent styryl pyridinium dyes FM1-43 and FM4-64 are used to probe activity-dependent synaptic vesicle cycling in neurons. Cultured astrocytes can internalize FM1-43 and FM4-64 inside vesicles but their uptake is insensitive to the elevation of cytosolic calcium (Ca(2+)) concentration and the under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li D,Hérault K,Oheim M,Ropert N

更新日期：2009-12-22 00:00:00

abstract：:Single-copy transgenes in Caenorhabditis elegans can be subjected to a potent, irreversible silencing process termed small RNA-induced epigenetic silencing (RNAe). RNAe is promoted by the Piwi Argonaute protein PRG-1 and associated Piwi-interacting RNAs (piRNAs), as well as by proteins that promote and respond to seco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leopold LE,Heestand BN,Seong S,Shtessel L,Ahmed S

更新日期：2015-05-19 00:00:00

abstract：:This paper reports the results of a large-scale field experiment designed to test the hypothesis that group membership can increase participation and prosocial lending for an online crowdlending community, Kiva. The experiment uses variations on a simple email manipulation to encourage Kiva members to join a lending t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ai W,Chen R,Chen Y,Mei Q,Phillips W

更新日期：2016-12-27 00:00:00

abstract：:Like protein enzymes, RNA enzymes (ribozymes) provide specific binding sites for their substrates. We now show that equilibrium dissociation constants for complexes between the Tetrahymena ribozyme and its RNA substrates and products can be directly measured by electrophoresis in polyacrylamide gels containing divalen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pyle AM,McSwiggen JA,Cech TR

更新日期：1990-11-01 00:00:00

abstract：:O-linked N-acetylglucosamine (GlcNAc) transferase (OGT) is the only known enzyme that catalyzes the O-GlcNAcylation of proteins at the Ser or Thr side chain hydroxyl group. OGT participates in transcriptional and epigenetic regulation, and dysregulation of OGT has been implicated in diseases such as cancer. However, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu CS,Lo PW,Yeh YH,Hsu PH,Peng SH,Teng YC,Kang ML,Wong CH,Juan LJ

更新日期：2014-01-28 00:00:00

abstract：:Replication-defective human adenovirus (Ad) group C transducing vectors, most of which have the E1A, E1B, and E3 genes deleted, are highly inflammatory despite the fact that the parental viruses typically cause subclinical or mild infections. To investigate this paradox, the roles that the E1A, E1B, and E3 genes play ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaack J,Bennett ML,Colbert JD,Torres AV,Clayton GH,Ornelles D,Moorhead J

更新日期：2004-03-02 00:00:00

abstract：:Mechanisms underlying age-related obesity and insulin resistance are generally unknown. Here, we report age-related adipose vascular changes markedly modulated fat mass, adipocyte functions, blood lipid composition, and insulin sensitivity. Notably, VEGF expression levels in various white adipose tissues (WATs) underw...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honek J,Seki T,Iwamoto H,Fischer C,Li J,Lim S,Samani NJ,Zang J,Cao Y

更新日期：2014-10-14 00:00:00

abstract：:We have screened a human DNA library using as probe a chimpanzee sequence that contains homology to the polymerase gene of the endogenous baboon virus. One set of overlapping clones spans about 20 kilobases and contains regions of DNA sequence homology to the gag p30, gag p15, and polymerase genes of Moloney murine le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonner TI,O'Connell C,Cohen M

更新日期：1982-08-01 00:00:00

abstract：:We describe retroviral expression vectors containing cDNAs encoding part of fibronectin preceded by the signal and "pro" sequences of parathyroid hormone. The recombinant retroviruses were used to generate NIH 3T3 cell lines stably producing functionally active fragments of fibronectin. The recombinant fibronectins (d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwarzbauer JE,Mulligan RC,Hynes RO

更新日期：1987-02-01 00:00:00

abstract：:Horizontal cells of the carp retina possess dopamine receptors linked to adenylate cyclase. Isolated, intact horizontal cells respond to micromolar concentrations of dopamine, whereas nanomolar concentrations of haloperidol, (+)-butaclamol, and flupenthixol block the dopamine response. Preincubation in Ringer's soluti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Buskirk R,Dowling JE

更新日期：1982-05-01 00:00:00

abstract：:This report provides direct evidence that strigolactone (SL) positively regulates drought and high salinity responses in Arabidopsis. Both SL-deficient and SL-response [more axillary growth (max)] mutants exhibited hypersensitivity to drought and salt stress, which was associated with shoot- rather than root-related t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ha CV,Leyva-González MA,Osakabe Y,Tran UT,Nishiyama R,Watanabe Y,Tanaka M,Seki M,Yamaguchi S,Dong NV,Yamaguchi-Shinozaki K,Shinozaki K,Herrera-Estrella L,Tran LS

更新日期：2014-01-14 00:00:00

abstract：:Two major themes in the physics of condensed matter are quantum critical phenomena and unconventional superconductivity. These usually occur in the context of competing interactions in systems of strongly correlated electrons. All this interesting physics comes together in the behavior of the recently discovered iron ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai J,Si Q,Zhu JX,Abrahams E

更新日期：2009-03-17 00:00:00