Abstract:
AIM:To investigate the role of TIP30 in apoptotic signal pathway in hepatoblastoma cells and to provide a basis for TIP30 as a gene therapy candidate in the regression of hepatoblastoma cells. METHODS:Apoptosis of human hepatoblastoma cell lines HepG2 (p53 wild), Hep3B (p53 null) and PLC/RPF/5 (p53 mutant) infected with Ad-TIP30 (bearing a wild type human Tip30 gene) were analyzed and p53, Bax and Bcl-xl expression levels were compared among these cells. MTT assay, DNA fragmentation, in situ 3' end labeling of DNA, annexin-V FITC staining were used to detect cell death and apoptosis in cells at various time intervals subsequent to infection, and to determine whether TIP30 had an effect on the expression levels of some apoptosis-related gene products such as Bax, p53 and Bcl-xl. A similar time course experiment was performed by Western blotting. RESULTS:In MTT assay, the viability of HepG2 cells decreased significantly from 99.7% to 10% and displayed more massive cell death within 5-8 d than Hep3B and PLC/RPF/5 cells, with their viability decreased from 97.8% to 44.3% and 98.1% to 50.4%, respectively. In annexin-V FITC assay, the percentage of apoptosis cells in HepG2 cells was two to three-fold higher than that in control cells (infected with Ad-GFP), two-fold higher than that in Hep3B cells and 1.4-fold higher than that in PLC/RPF/5 cells 36 h after infection, respectively. Moreover, in HepG2 cells, the p53 began to increase 6-8 h after infection, reaching a maximum level between 8 and 12 h after infection and then dropped. Bax showed a similar increase in the cells as p53 reached the maximum at 8-12 h and subsequently decreased. Interestingly, Bcl-xl protein levels were down regulated during 24 to 36 h after Ad-TIP30 infection. In contrast, ectopic expression of TIP30 in Hep3B and PLC/RPF/5 cells had no effect on the regulation of Bax expression, but had an effect on Bcl-xl levels. In comparison with HepG2 cells, these data suggested that up-regulation of p53 levels by TIP30 might be a pre-requisite for Bax and Bax/Bcl-xl ratio increase. We hypothesized that TIP30 might regulate Bax gene partly through p53, which sensitizes cells to apoptosis by involving a p53 apoptosis signal transduction pathway. CONCLUSION:TIP30 plays an important role in predisposing hepatoblastoma cells to apoptosis through regulating expression levels of these genes. Ad-TIP30 carrying exogenous TIP30-anti-tumor genes may be regarded as a potential candidate for the treatment of hepatocellular carcinoma.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Shi M,Zhang X,Wang P,Zhang HW,Zhang BH,Wu MCdoi
10.3748/wjg.v11.i2.221keywords:
subject
Has Abstractpub_date
2005-01-14 00:00:00pages
221-7issue
2eissn
1007-9327issn
2219-2840journal_volume
11pub_type
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.3748/wjg.v23.i34.6330
更新日期:2017-09-14 00:00:00
abstract::AIM:To further clarify the clinicopathological, immunohistochemical and electron microscopic features, and prognostic aspect of basaloid squamous carcinoma (BSC), a rare esophageal carcinoma.METHODS:We reviewed 763 documented cases of esophageal malignancies from year (1977-1996) from our hospital, and discovered 16 (...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v4.i5.397
更新日期:1998-10-01 00:00:00
abstract::Liver regeneration after major surgery may activate occult micrometastases and facilitate tumor growth, leading to liver tumor recurrence. Molecular changes during liver regeneration can provide a microenvironment that stimulates intrahepatic tumor propagation through alterations in cellular signaling pathways, where ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i43.16167
更新日期:2014-11-21 00:00:00
abstract:AIM:To investigate long-term outcome in obscure gastrointestinal bleeding (OGIB) after negative capsule endoscopy (CE) and identify risk factors for rebleeding. METHODS:A total of 113 consecutive patients underwent CE for OGIB from May 2003 to June 2010 at Seoul National University Hospital. Ninety-five patients (84.1...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v19.i10.1632
更新日期:2013-03-14 00:00:00
abstract:AIM:To investigate the hotspots, direction, and the time course of evolution of hepatitis A virus in the process of consecutive cell culture passage in human KMB17 diploid cells. METHODS:Wild type hepatitis A virus H2w was serially propagated in KMB17 cells until passage 30, and the full-length genomes of H2w and its ...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v13.i34.4630
更新日期:2007-09-14 00:00:00
abstract::A 52 year-old male patient diagnosed of ankylosing spondylitis presented with an iron deficiency anemia after a ten-month treatment of methotrexate. He did not respond to treatment with oral iron not a proton pump inhibitor and an upper endoscopy was performed. The histological study of the duodenal biopsies showed vi...
journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.7009
更新日期:2008-12-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.14.1370
更新日期:2008-03-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v17.i13.1701
更新日期:2011-04-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v20.i11.2771
更新日期:2014-03-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i11.3239
更新日期:2015-03-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i3.340
更新日期:2005-01-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.3748/wjg.v17.i34.3916
更新日期:2011-09-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v23.i1.48
更新日期:2017-01-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v21.i5.1404
更新日期:2015-02-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i15.1837
更新日期:2010-04-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v24.i30.3414
更新日期:2018-08-14 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i36.5096
更新日期:2012-09-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v26.i15.1733
更新日期:2020-04-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.15.5067
更新日期:2009-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v22.i15.3969
更新日期:2016-04-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v21.i40.11458
更新日期:2015-10-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 临床试验,杂志文章
doi:10.3748/wjg.v11.i8.1109
更新日期:2005-02-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i45.17155
更新日期:2014-12-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v20.i3.774
更新日期:2014-01-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.3748/wjg.v20.i11.2995
更新日期:2014-03-21 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v11.i29.4570
更新日期:2005-08-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i20.2526
更新日期:2012-05-28 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v18.i5.445
更新日期:2012-02-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
doi:10.3748/wjg.v26.i17.1993
更新日期:2020-05-07 00:00:00
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2017-02-07 00:00:00