Freeze-fracture morphology and quantification of human bronchial epithelial tight junctions.

abstract：:Bombesin-like peptides within neuroepithelial cells elicit proliferation of normal and malignant airway epithelial cells. It is not clear that these peptides also elicit epithelial cell migration, a necessary component of airway repair after injury. We studied the effects of the bombesin analogues, gastrin releasing p...

journal_title：American journal of respiratory cell and molecular biology

authors： Kim JS,McKinnis VS,White SR

更新日期：1997-03-01 00:00:00

abstract：:We examined the effects of extracellular ATP on the proliferation and synthesis of DNA by guinea pig alveolar macrophages (AM). AM proliferated spontaneously in vitro, their number doubling in 72 h. Such proliferation was completely inhibited by adding 1 mM ATP to the culture. The inhibition was dose dependent. ATP al...

journal_title：American journal of respiratory cell and molecular biology

authors： Nakanishi M,Kawasaki M,Ogino H,Yoshida M,Yagawa K

更新日期：1994-05-01 00:00:00

abstract：:Cigarette smoking and aging are major risk factors for chronic obstructive pulmonary disease. An unsolved question is whether elderly lungs are particularly vulnerable to cigarette smoke (CS) exposure. In this study, we used a mouse model to test the hypothesis that aging increases the susceptibility to CS-induced pul...

journal_title：American journal of respiratory cell and molecular biology

authors： Moriyama C,Betsuyaku T,Ito Y,Hamamura I,Hata J,Takahashi H,Nasuhara Y,Nishimura M

更新日期：2010-03-01 00:00:00

abstract：:Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid that plays an important proinflammatory role in asthmatic airways. Corticosteroids are first-line antiinflammatories in asthma; however, their repressive effects on S1P-induced cytokine secretion have not been investigated. To address this, our in vitro study r...

journal_title：American journal of respiratory cell and molecular biology

authors： Che W,Parmentier J,Seidel P,Manetsch M,Ramsay EE,Alkhouri H,Ge Q,Armour CL,Ammit AJ

更新日期：2014-02-01 00:00:00

abstract：:Interleukin-13 (IL-13) is one of the central mediators for development of airway hyperresponsiveness in asthma. However, its effect on bronchial smooth muscle (BSM) is not well known. Recent studies revealed an involvement of RhoA/Rho-kinase in BSM contraction, and this pathway has now been proposed as a new target fo...

journal_title：American journal of respiratory cell and molecular biology

authors： Chiba Y,Nakazawa S,Todoroki M,Shinozaki K,Sakai H,Misawa M

更新日期：2009-02-01 00:00:00

abstract：:LL-37 is a human cationic host defense peptide that is present in the specific granules of neutrophils, produced by epithelial cells from a variety of tissues, and is upregulated during inflammation, infection, and injury. It has been proposed to have a variety of antimicrobial functions, including both direct antimic...

journal_title：American journal of respiratory cell and molecular biology

authors： Lau YE,Bowdish DM,Cosseau C,Hancock RE,Davidson DJ

更新日期：2006-04-01 00:00:00

abstract：:Dendritic cells (DCs) are essential for innate and adaptive immunity, but are purported to exhibit variable radiosensitivity in response to irradiation in various bone marrow transplantation (BMT) protocols. To address this controversy, we analyzed the magnitude of depletion and repopulation of both lung CD11b(pos) DC...

journal_title：American journal of respiratory cell and molecular biology

authors： Hahn I,Klaus A,Maus R,Christman JW,Welte T,Maus UA

更新日期：2011-09-01 00:00:00

abstract：:The effects of concomitant P1-receptor stimulation on peak intracellular Ca2+ release by extracellular adenosine 5'-triphosphate (ATP) and 5-hydroxytryptamine (5-HT) were investigated in cultured airway smooth-muscle (ASM) cells. The results show that peak Ca2+ release to ATP is enhanced by preincubation with adenosin...

journal_title：American journal of respiratory cell and molecular biology

authors： Michoud MC,Tao FC,Pradhan AA,Martin JG

更新日期：1999-07-01 00:00:00

abstract：:We have previously identified two integrins, alpha 9 beta 1 and alpha v beta 6, from guinea pig airway epithelium. The extracellular matrix protein tenascin is a ligand for both of these receptors, and fibronectin is also a ligand for alpha v beta 6. In the present study, we used immunohistochemistry to examine the ex...

journal_title：American journal of respiratory cell and molecular biology

authors： Weinacker A,Ferrando R,Elliott M,Hogg J,Balmes J,Sheppard D

更新日期：1995-05-01 00:00:00

abstract：:Myosin light chain kinase (MLCK; gene code, MYLK) is a multifunctional enzyme involved in isoform-specific nonmuscle (nm) and smooth muscle contraction, inflammation, and vascular permeability, processes directly relevant to asthma pathobiology. In this report, we highlight the contribution of the nm isoform (nmMLCK) ...

journal_title：American journal of respiratory cell and molecular biology

authors： Wang T,Moreno-Vinasco L,Ma SF,Zhou T,Shimizu Y,Sammani S,Epshtein Y,Watterson DM,Dudek SM,Garcia JG

更新日期：2014-06-01 00:00:00

abstract：:Pseudomonas aeruginosa colonizes the lower respiratory tracts of patients with severe bronchiectasis, including cystic fibrosis, a condition associated with increased airway mucus output. We have shown that an extract containing chloroform-soluble extracellular products of P. aeruginosa releases glycoconjugates into t...

journal_title：American journal of respiratory cell and molecular biology

authors： Somerville M,Taylor GW,Watson D,Rendell NB,Rutman A,Todd H,Davies JR,Wilson R,Cole P,Richardson PS

更新日期：1992-01-01 00:00:00

abstract：:Rat alveolar type II pneumocytes, in situ, label with Maclura pomifera agglutinin (MPA), a plant lectin that recognizes alpha-galactosyl oligosaccharide residues of glycoproteins and glycolipids. To study the glycoproteins recognized by the lectin, MPA lectin affinity chromatography was used to isolate a novel glycopr...

journal_title：American journal of respiratory cell and molecular biology

authors： Lwebuga-Mukasa JS

更新日期：1991-06-01 00:00:00

abstract：:Adenosine concentrations are elevated in the lungs of patients with asthma and chronic obstructive pulmonary disease, where it balances between tissue repair and excessive airway remodeling. We previously demonstrated that the activation of the adenosine A2A receptor promotes epithelial wound closure. However, the mec...

journal_title：American journal of respiratory cell and molecular biology

authors： Allen-Gipson DS,Zimmerman MC,Zhang H,Castellanos G,O'Malley JK,Alvarez-Ramirez H,Kharbanda K,Sisson JH,Wyatt TA

更新日期：2013-05-01 00:00:00

abstract：:Airway neutrophilia is a prominent feature of chronic obstructive pulmonary disease. As cigarette smoke (CS) and epidermal growth factor (EGF) both cause release of interleukin-8 (IL-8) from epithelial cells in vitro, we investigated whether autocrine ligands for the EGF receptor (EGFR) are involved in this proinflamm...

journal_title：American journal of respiratory cell and molecular biology

authors： Richter A,O'Donnell RA,Powell RM,Sanders MW,Holgate ST,Djukanović R,Davies DE

更新日期：2002-07-01 00:00:00

abstract：:We studied the effect of the nitric oxide synthase (NOS) inhibitor asymmetric dimethyl arginine (ADMA) and the inactive enantiomer N G-methyl-D-arginine (D-NMMA) on Pseudomonas aeruginosa infection of the respiratory mucosa in nasal turbinate organ cultures. We also investigated the effect of P. aeruginosa culture fil...

journal_title：American journal of respiratory cell and molecular biology

authors： Dowling RB,Newton R,Robichaud A,Cole PJ,Barnes PJ,Wilson R

更新日期：1998-12-01 00:00:00

abstract：:Cigarette smoke (CS) is the main causative factor of chronic obstructive pulmonary disease (COPD). Current research supports the concept that airway inflammation is central to the development and progression of the disease. Studies have demonstrated that neutrophils are increased in COPD lungs and that neutrophil-asso...

journal_title：American journal of respiratory cell and molecular biology

authors： Cardini S,Dalli J,Fineschi S,Perretti M,Lungarella G,Lucattelli M

更新日期：2012-09-01 00:00:00

abstract：:We sought to determine whether an intact bone marrow is essential to lung repair following bleomycin-induced lung injury in mice, and the mechanisms of any protective effects conferred by bone marrow-derived mesenchymal stem cell (BMDMSC) transfer. We found that myelosupression increased susceptibility to bleomycin in...

journal_title：American journal of respiratory cell and molecular biology

authors： Rojas M,Xu J,Woods CR,Mora AL,Spears W,Roman J,Brigham KL

更新日期：2005-08-01 00:00:00

abstract：:Recent evidence from genetic experiments in yeast and from studies using guanosine triphosphate (GTP) analogues in mammalian cells suggests a key role for low-molecular-mass GTP-binding proteins (LMM-GBPs) (Mr 19 to 28 kD) in processes of intracellular vesicular sorting and secretion. Assembly and exocytosis of the la...

journal_title：American journal of respiratory cell and molecular biology

authors： Rubins JB,Panchenko M,Shannon TM,Dickey BF

更新日期：1992-03-01 00:00:00

abstract：:Mucus hypersecretion is characteristic of chronic airway diseases. However, regulatory mechanisms are poorly understood. Human airway epithelial cells grown on permeable supports at the air-liquid interface (ALI) develop a mucociliary morphology resembling that found in vivo. Such cultures provide a model for studying...

journal_title：American journal of respiratory cell and molecular biology

authors： Bernacki SH,Nelson AL,Abdullah L,Sheehan JK,Harris A,Davis CW,Randell SH

更新日期：1999-04-01 00:00:00

abstract：:Laminins are heterotrimeric proteins that are secreted by the alveolar epithelium into the basement membrane, and their expression is altered in extracellular matrices from patients with pulmonary fibrosis. In a small number of patients with pulmonary fibrosis, we found that the normal basement membrane distribution o...

journal_title：American journal of respiratory cell and molecular biology

authors： Morales-Nebreda LI,Rogel MR,Eisenberg JL,Hamill KJ,Soberanes S,Nigdelioglu R,Chi M,Cho T,Radigan KA,Ridge KM,Misharin AV,Woychek A,Hopkinson S,Perlman H,Mutlu GM,Pardo A,Selman M,Jones JC,Budinger GR

更新日期：2015-04-01 00:00:00

abstract：:Development of gene transfer vectors with regulated, lung-specific expression will be a useful tool for studying lung biology and developing gene therapies. In this study we constructed a series of lentiviral vectors with regulatory elements predicted to produce lung-specific transgene expression: the surfactant prote...

journal_title：American journal of respiratory cell and molecular biology

authors： Hendrickson B,Senadheera D,Mishra S,Bui KC,Wang X,Chan B,Petersen D,Pepper K,Lutzko C

更新日期：2007-10-01 00:00:00

abstract：:A defining feature of acute lung injury (ALI) is the increased lung vascular permeability and alveolar flooding, which leads to associated morbidity and mortality. Specific therapies to alleviate the unremitting vascular leak in ALI are not currently clinically available; however, our prior studies indicate a protecti...

journal_title：American journal of respiratory cell and molecular biology

authors： Zhao Y,Gorshkova IA,Berdyshev E,He D,Fu P,Ma W,Su Y,Usatyuk PV,Pendyala S,Oskouian B,Saba JD,Garcia JG,Natarajan V

更新日期：2011-08-01 00:00:00

abstract：:The hydroxyl radical (.OH) is a highly reactive oxygen free radical that has been implicated as a cause of lung injury following exposure to silica and silicates. Despite evidence that silica generates .OH in vitro, there has been no previous demonstration of in vivo production of .OH after exposure to nonfibrous mine...

journal_title：American journal of respiratory cell and molecular biology

authors： Schapira RM,Ghio AJ,Effros RM,Morrisey J,Almagro UA,Dawson CA,Hacker AD

更新日期：1995-02-01 00:00:00

abstract：:Airway infections or irritant exposures during early postnatal periods may contribute to the onset of childhood asthma. The purpose of this study was to examine critical periods of postnatal airway development during which ozone (O(3)) exposure leads to heightened neural responses. Rats were exposed to O(3) (2 ppm) or...

journal_title：American journal of respiratory cell and molecular biology

authors： Hunter DD,Wu Z,Dey RD

更新日期：2010-12-01 00:00:00

abstract：:While elastin degradation is a hallmark of pulmonary emphysema, it is likely that elastin synthesis also occurs. However, the supramolecular structure and function of the newly synthesized elastin are abnormal. Very little is known about the regulation of elastin synthesis during the development of emphysema when prom...

journal_title：American journal of respiratory cell and molecular biology

authors： McGowan SE,McNamer R

更新日期：1990-10-01 00:00:00

abstract：:The lung is richly supplied with peptidergic nerves that store and secrete substance P (SP), vasoactive intestinal peptide (VIP), and other neuropeptides known to potently modulate leukocyte function in vitro and airway inflammation in vivo. To investigate and characterize neuromodulation of immune responses compartme...

journal_title：American journal of respiratory cell and molecular biology

authors： Kaltreider HB,Ichikawa S,Byrd PK,Ingram DA,Kishiyama JL,Sreedharan SP,Warnock ML,Beck JM,Goetzl EJ

更新日期：1997-02-01 00:00:00

abstract：:Bleomycin-induced lung injury causes increased fibroblast numbers in the lung and pulmonary fibrosis. Studies of fibroblasts isolated from such injured lungs have revealed evidence of increased intrinsic proliferative capacity, but the mechanism is unknown. Telomerase catalyzes the addition of telomeric DNA repeats on...

journal_title：American journal of respiratory cell and molecular biology

authors： Nozaki Y,Liu T,Hatano K,Gharaee-Kermani M,Phan SH

更新日期：2000-10-01 00:00:00

abstract：:Polymorphonuclear leukocytes (PMN) and eosinophils (Eos) are important cellular participants in a variety of acute and chronic inflammatory reactions in the airway. Histologic evidence has implicated direct interactions between these two subsets of leukocytes and airway epithelial cells during inflammation. A comprehe...

journal_title：American journal of respiratory cell and molecular biology

authors： Jagels MA,Daffern PJ,Zuraw BL,Hugli TE

更新日期：1999-09-01 00:00:00

abstract：:Long-term success in lung transplantation is limited by obliterative bronchiolitis, whereas T cell effector mechanisms in this process remain incompletely understood. Using the mouse heterotopic allogeneic airway transplant model, we studied T cell effector responses during obliterative airways disease (OAD). Allospec...

journal_title：American journal of respiratory cell and molecular biology

authors： West EE,Lavoie TL,Orens JB,Chen ES,Ye SQ,Finkelman FD,Garcia JG,McDyer JF

更新日期：2006-01-01 00:00:00

abstract：:In this study, we have used the rat model of hyperoxia to examine the molecular responses to oxidative stress in lung. We show that in addition to the antioxidant enzyme manganese superoxide dismutase, expression of a variety of stress-responsive genes including heme oxygenase-1, c-fos, c-jun, CAAT-enhancer binding pr...

journal_title：American journal of respiratory cell and molecular biology

authors： Choi AM,Sylvester S,Otterbein L,Holbrook NJ

更新日期：1995-07-01 00:00:00