Molecular responses to hyperoxia in vivo: relationship to increased tolerance in aged rats.

abstract：:The doxycycline-inducible, gene regulatory system allows tight control of transgene expression for the study of organ development and disease pathogenesis. Multiple recent reports have employed this model to investigate various lung diseases including emphysema. For our study, we used this transgenic system to test wh...

journal_title：American journal of respiratory cell and molecular biology

authors： Sisson TH,Hansen JM,Shah M,Hanson KE,Du M,Ling T,Simon RH,Christensen PJ

更新日期：2006-05-01 00:00:00

abstract：:Transforming growth factor-beta (TGF-beta) is a cytokine implicated in wound healing and in the pathogenesis of pulmonary fibrosis. TGF-beta stimulates myofibroblast differentiation characterized by expression of contractile smooth muscle (SM)-specific proteins such as SM-alpha-actin. In the present study, we examined...

journal_title：American journal of respiratory cell and molecular biology

authors： Sandbo N,Kregel S,Taurin S,Bhorade S,Dulin NO

更新日期：2009-09-01 00:00:00

abstract：:Excessive growth of pulmonary arterial (PA) smooth muscle cells (SMCs) is a major component of PA hypertension (PAH). The calcium-activated neutral cysteine proteases calpains 1 and 2, expressed by PASMCs, contribute to PH but are tightly controlled by a single specific inhibitor, calpastatin. Our objective was to inv...

journal_title：American journal of respiratory cell and molecular biology

authors： Wan F,Letavernier E,Abid S,Houssaini A,Czibik G,Marcos E,Rideau D,Parpaleix A,Lipskaia L,Amsellem V,Gellen B,Sawaki D,Derumeaux G,Dubois-Randé JL,Delcroix M,Quarck R,Baud L,Adnot S

更新日期：2016-09-01 00:00:00

abstract：:Ground-level ozone (O3) is a ubiquitous environmental air pollutant that is a potent inducer of airway inflammation and has been linked with respiratory and cardiovascular morbidity and mortality. Some studies using transformed or immortalized cells have attributed O3-mediated expression of inflammatory cytokines with...

journal_title：American journal of respiratory cell and molecular biology

authors： McCullough SD,Duncan KE,Swanton SM,Dailey LA,Diaz-Sanchez D,Devlin RB

更新日期：2014-09-01 00:00:00

abstract：:Zn may have an important protective role in the respiratory epithelium and Zn deficiency may enhance airway inflammation and epithelial damage. The effects of mild nutritional Zn deficiency on airway hyperresponsiveness (AHR) and airway inflammation in mice sensitized and challenged with ovalbumin (OVA) to induce an a...

journal_title：American journal of respiratory cell and molecular biology

authors： Truong-Tran AQ,Ruffin RE,Foster PS,Koskinen AM,Coyle P,Philcox JC,Rofe AM,Zalewski PD

更新日期：2002-09-01 00:00:00

abstract：:The present study utilized the monocrotaline (MCT) model of pulmonary hypertension in rats to examine temporal alterations in steady-state levels of basement membrane (BM) component mRNA and deposition of protein using Northern analysis and immunohistochemistry, respectively. MCT (60 mg/kg, subcutaneous) produced sust...

journal_title：American journal of respiratory cell and molecular biology

authors： Lipke DW,Arcot SS,Gillespie MN,Olson JW

更新日期：1993-10-01 00:00:00

abstract：:Heritable pulmonary arterial hypertension (HPAH) is a serious lung vascular disease caused by heterozygous mutations in the bone morphogenetic protein (BMP) pathway genes, BMPR2 and SMAD9. One noncanonical function of BMP signaling regulates biogenesis of a subset of microRNAs. We have previously shown that this funct...

journal_title：American journal of respiratory cell and molecular biology

authors： Drake KM,Dunmore BJ,McNelly LN,Morrell NW,Aldred MA

更新日期：2013-09-01 00:00:00

abstract：:Acute lung injury (ALI) and its severe form, acute respiratory distress syndrome (ARDS), are major causes of acute respiratory failure with high rates of morbidity and mortality. Although surfactant protein (SP)-D plays a critical role in pulmonary innate immunity and several clinical studies suggest that this protein...

journal_title：American journal of respiratory cell and molecular biology

authors： King BA,Kingma PS

更新日期：2011-05-01 00:00:00

abstract：:MyD88 is an adapter protein required for the induction of proinflammatory cytokines by most Toll-like receptors (TLR), and Pseudomonas aeruginosa expresses ligands for multiple TLRs. MyD88(-/-) (KO) mice are highly susceptible to aerosolized P. aeruginosa, failing to elicit an early inflammatory response and permittin...

journal_title：American journal of respiratory cell and molecular biology

authors： Hajjar AM,Harowicz H,Liggitt HD,Fink PJ,Wilson CB,Skerrett SJ

更新日期：2005-11-01 00:00:00

abstract：:Laminins are heterotrimeric proteins that are secreted by the alveolar epithelium into the basement membrane, and their expression is altered in extracellular matrices from patients with pulmonary fibrosis. In a small number of patients with pulmonary fibrosis, we found that the normal basement membrane distribution o...

journal_title：American journal of respiratory cell and molecular biology

authors： Morales-Nebreda LI,Rogel MR,Eisenberg JL,Hamill KJ,Soberanes S,Nigdelioglu R,Chi M,Cho T,Radigan KA,Ridge KM,Misharin AV,Woychek A,Hopkinson S,Perlman H,Mutlu GM,Pardo A,Selman M,Jones JC,Budinger GR

更新日期：2015-04-01 00:00:00

abstract：:Endothelin-1 (ET-1) exerts many biological effects in airways, including bronchoconstriction, airway mucus secretion, cell proliferation, and inflammation. We investigated the effect of ET-1 on Na absorption and Cl secretion in human bronchial epithelial cells. Addition of 10(-7) M ET-1 had no effect on the inhibition...

journal_title：American journal of respiratory cell and molecular biology

authors： Blouquit S,Sari A,Lombet A,D'herbomez M,Naline E,Matran R,Chinet T

更新日期：2003-08-01 00:00:00

abstract：:Administration of supplemental oxygen remains a critical clinical intervention for survival of preterm infants with respiratory failure. However, prolonged exposure to hyperoxia can augment pulmonary damage, resulting in developmental lung diseases embodied as hyperoxia-induced acute lung injury and bronchopulmonary d...

journal_title：American journal of respiratory cell and molecular biology

authors： Sureshbabu A,Syed M,Das P,Janér C,Pryhuber G,Rahman A,Andersson S,Homer RJ,Bhandari V

更新日期：2016-11-01 00:00:00

abstract：:An NADPH-oxidase complex containing at least two protein components (gp91-phox and p22-phox) and a unique low redox potential (-245 mV) cytochrome b-245 is the source of superoxide generated for bacterial killing in neutrophils and has been suggested as the oxygen sensor in the carotid body. In pure cultures of smooth...

journal_title：American journal of respiratory cell and molecular biology

authors： Marshall C,Mamary AJ,Verhoeven AJ,Marshall BE

更新日期：1996-11-01 00:00:00

abstract：:Endogenous hydrogen peroxide (H2O2) release from aortic endothelial cells was studied in the presence of antioxidant enzyme inhibitors, mitochondrial inhibitors, a microsomal cytochrome P-450 inhibitor, and after oxidative stress induced with H2O2 or menadione. Extracellular H2O2 generation was determined spectrofluor...

journal_title：American journal of respiratory cell and molecular biology

authors： Kinnula VL,Whorton AR,Chang LY,Crapo JD

更新日期：1992-02-01 00:00:00

abstract：:Notch is an ancient cell-signaling system that regulates the specification of cell fate. This study examined the role of Notch in the epithelial-mesenchymal transition (EMT) and myofibroblast differentiation of cultured RLE-6TN cells (i.e., rat alveolar epithelial cells). The activation of Notch, either by ectopic exp...

journal_title：American journal of respiratory cell and molecular biology

authors： Aoyagi-Ikeda K,Maeno T,Matsui H,Ueno M,Hara K,Aoki Y,Aoki F,Shimizu T,Doi H,Kawai-Kowase K,Iso T,Suga T,Arai M,Kurabayashi M

更新日期：2011-07-01 00:00:00

abstract：:Mycobacterium avium (MAV) and M. abscessus (MAB) are ubiquitous environmental organisms increasingly recognized to cause chronic lung disease in patients with apparently normal immune function. Little is yet known about their human pathophysiology. Our objective was to examine cytokine and chemokine responses (protein...

journal_title：American journal of respiratory cell and molecular biology

authors： Sampaio EP,Elloumi HZ,Zelazny A,Ding L,Paulson ML,Sher A,Bafica AL,Shea YR,Holland SM

更新日期：2008-10-01 00:00:00

abstract：:Platelet-activating factor (PAF), a proinflammatory lipid mediator, is a potent airway mucin secretagogue. This study assessed the role of protein kinase C (PKC) in PAF-induced mucin release from primary cultures of feline tracheal epithelial cells (FTEC). Mucin secretion was quantitated by enzyme-linked immunosorbent...

journal_title：American journal of respiratory cell and molecular biology

authors： Larivée P,Levine SJ,Martinez A,Wu T,Logun C,Shelhamer JH

更新日期：1994-08-01 00:00:00

abstract：:Previous observations by our laboratory indicate that the presence of anti-IL-8 autoantibody:IL-8 immune complexes in lung fluids from patients with acute lung injury/acute respiratory distress syndrome (ALI/ARDS) comprises an important prognostic indicator in the development and ultimate outcome of ALI/ARDS. We also ...

journal_title：American journal of respiratory cell and molecular biology

authors： Krupa A,Fudala R,Florence JM,Tucker T,Allen TC,Standiford TJ,Luchowski R,Fol M,Rahman M,Gryczynski Z,Gryczynski I,Kurdowska AK

更新日期：2013-02-01 00:00:00

abstract：:Prenatal nicotine exposure impairs normal lung development and leads to diminished pulmonary function after birth. Previous work from our laboratory has demonstrated that nicotine alters lung development by affecting a nonneuronal cholinergic autocrine loop that is expressed in lung. Bronchial epithelial cells (BECs) ...

journal_title：American journal of respiratory cell and molecular biology

authors： Fu XW,Lindstrom J,Spindel ER

更新日期：2009-07-01 00:00:00

abstract：:Lung transplantation remains the only therapeutic option for many patients suffering from end-stage pulmonary disease. Long-term success after lung transplantation is severely limited by the development of bronchiolitis obliterans. The murine heterotopic tracheal transplantation model has been widely used for studies ...

journal_title：American journal of respiratory cell and molecular biology

authors： Okazaki M,Gelman AE,Tietjens JR,Ibricevic A,Kornfeld CG,Huang HJ,Richardson SB,Lai J,Garbow JR,Patterson GA,Krupnick AS,Brody SL,Kreisel D

更新日期：2007-12-01 00:00:00

abstract：:It has long been speculated that neutrophils deploy proteases to digest subendothelial matrix as they migrate from the bloodstream. Direct evidence for the involvement of proteases in neutrophil transendothelial migration is, however, lacking. To address this issue we used transmission electron microscopy to verify th...

journal_title：American journal of respiratory cell and molecular biology

authors： Mackarel AJ,Cottell DC,Russell KJ,FitzGerald MX,O'Connor CM

更新日期：1999-06-01 00:00:00

abstract：:Macrophage inflammatory proteins 1 alpha and 2 (MIP-1 alpha, MIP-2) are members of a growing family of cytokines thought to play a role in host defense. MIP-1 alpha and MIP-2 were previously identified in the mouse and shown to stimulate inflammatory cell recruitment. To better understand the potential role of MIP-1 a...

journal_title：American journal of respiratory cell and molecular biology

authors： Driscoll KE,Hassenbein DG,Carter J,Poynter J,Asquith TN,Grant RA,Whitten J,Purdon MP,Takigiku R

更新日期：1993-03-01 00:00:00

abstract：:The extracellular signal-regulated kinases 1 and 2 (ERK1/2) are phosphorylated after inhalation of asbestos. The effect of blocking this signaling pathway in lung epithelium is unclear. Asbestos-exposed transgenic mice expressing a dominant-negative mitogen-activated protein kinase kinase-1 (dnMEK1) (i.e., the upstrea...

journal_title：American journal of respiratory cell and molecular biology

authors： Manning CB,Sabo-Attwood T,Robledo RF,Macpherson MB,Rincón M,Vacek P,Hemenway D,Taatjes DJ,Lee PJ,Mossman BT

更新日期：2008-05-01 00:00:00

abstract：:Recent in vitro studies have suggested that interleukin-4 (IL-4) may be involved in the preferential migration of eosinophils into the airways in allergic asthma through its capacity to selectively increase vascular cell adhesion molecule-1 (VCAM-1) expression on vessels. To test this hypothesis, we studied the expres...

journal_title：American journal of respiratory cell and molecular biology

authors： Fukuda T,Fukushima Y,Numao T,Ando N,Arima M,Nakajima H,Sagara H,Adachi T,Motojima S,Makino S

更新日期：1996-01-01 00:00:00

abstract：:Although nitric oxide (NO) is known to inhibit vascular smooth muscle cell proliferation, the subcellular molecular mechanisms involved with the inhibitory signal transduction pathways are uncertain. We investigated the effect of exogenous NO on cell proliferation and the expression of p53, p21, and phosphorylated p42...

journal_title：American journal of respiratory cell and molecular biology

authors： Mizuno S,Kadowaki M,Demura Y,Ameshima S,Miyamori I,Ishizaki T

更新日期：2004-08-01 00:00:00

abstract：:Eotaxins-1, -2, and -3 mediate the recruitment of blood-borne eosinophils to allergically inflamed tissues by binding CC chemokine receptor (CCR) 3. Mast cells (MCs) are resident tissue cells that also express CCR3. In the present study, we demonstrate that human (h) MCs in nasal polyps and cultured cord blood-derived...

journal_title：American journal of respiratory cell and molecular biology

authors： Price KS,Friend DS,Mellor EA,De Jesus N,Watts GF,Boyce JA

更新日期：2003-04-01 00:00:00

abstract：:In rat models of Gram-negative pneumonia, pulmonary emigration of neutrophils (polymorphonuclear leukocytes [PMNs]) is blocked when rats are made endotoxemic by an intravenous administration of endotoxin (lipopolysaccharide [LPS]). To test whether dysfunctional PMN migratory responses in the endotoxemic rat are specif...

journal_title：American journal of respiratory cell and molecular biology

authors： Wagner JG,Driscoll KE,Roth RA

更新日期：1999-04-01 00:00:00

abstract：:Pleural infection is common. Evacuation of infected pleural fluid is essential for successful treatment, but it is often difficult because of adhesions/loculations within the effusion and the viscosity of the fluid. Intrapleural delivery of tissue plasminogen activator (tPA) (to break the adhesions) and deoxyribonucle...

journal_title：American journal of respiratory cell and molecular biology

authors： Lansley SM,Cheah HM,Varano Della Vergiliana JF,Chakera A,Lee YC

更新日期：2015-07-01 00:00:00

abstract：:Alveolar type II cells produce pulmonary surfactant and serve as the stem cell of the alveolar epithelium by proliferating and transforming into type I cells. The study of the differentiated function and proliferative capacity of type II cells in response to injury in vivo has been hindered by the complexity of the sy...

journal_title：American journal of respiratory cell and molecular biology

authors： Kinnard WV,Tuder R,Papst P,Fisher JH

更新日期：1994-10-01 00:00:00

abstract：:PS-341 is a highly selective and potent proteasome inhibitor that is cytotoxic to various types of cancer. However, no objective response was seen in a clinical trial with PS-341 as a single agent in non-small cell lung cancer. Its antitumor activity is limited by the simultaneously activated antiapoptosis pathway. Re...

journal_title：American journal of respiratory cell and molecular biology

authors： Lee KH,Jang AH,Yoo CG

更新日期：2015-09-01 00:00:00