Abstract:
:The most potent carcinogen of the cyclopenta[a]phenanthrene series, 15, 16-dihydro-11-methylcyclopenta[a]phenanthren-17-one and its non-carcinogenic, unmethylated parent compound, were compared for their abilities to induce micronuclei in epidermal keratinocytes after application onto the dorsal skin of Skh/HR-1 hairless mice. Although both substances were shown to be mutagenic in vitro, only the 11-methyl derivative has been proven to initiate cancer in TO and Sencar mouse strains. In the present study, only the 11-methyl derivative was active as a cancer initiator in Skh/HR-1 mice. For studying micronucleus induction, a preliminary experiment was conducted to establish doses of both chemicals that allowed cell survival. Subsequently, micronucleus induction in epidermal keratinocytes was shown to agree with the cancer-initiating potential of the two compounds. Only the carcinogenic derivative induced a statistically significant increase in micronuclei, over the range 10-100 nmol. This is considerably lower than the dose of approximately 1600 nmol commonly used to initiate skin cancer in mice, but is comparable to the active dose range for skin micronucleus induction by benzo[a]pyrene, a chemical of equivalent carcinogenic potency.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Baker RS,Bonin AM,Arlauskas A,He S,Coombs MMdoi
10.1093/carcin/13.3.329keywords:
subject
Has Abstractpub_date
1992-03-01 00:00:00pages
329-32issue
3eissn
0143-3334issn
1460-2180journal_volume
13pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Mammalian DNA contains bulky endogenous DNA modifications (I-compounds), which increase with age in unexposed animals, as shown by 32P-postlabeling. We have examined the perinatal formation of a subclass (type II) of I-compounds in rat liver, kidney, skin and lung. These I-compounds represent bulky oxidative DNA lesio...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.12.2563
更新日期:1996-12-01 00:00:00
abstract::5-Aminolevulinic acid (ALA), a heme precursor accumulated in chemical and inborn porphyrias, may behave as an endogenous pro-oxidant. In chronically treated rats (40 mg ALA/kg body wt every 2 days for 15 days) the steady-state level of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in liver DNA (94.5 +/- 23.3 residues/10(6) dG)...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.10.2241
更新日期:1994-10-01 00:00:00
abstract::Sphingadienes (SDs) derived from soy and other natural sphingolipids are cytotoxic to colon cancer cells via an Akt-dependent mechanism and reduce adenoma formation in Apc(Min/+) mice. Wnt signaling is fundamental to colon carcinogenesis and is the basis for spontaneous tumorigenesis in Apc(Min/+) mice and patients wi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs174
更新日期:2012-09-01 00:00:00
abstract::A dose-dependent and transiently elevated expression of a cytoplasmic 32 kDa protein was observed in Swiss albino 3T3 fibroblasts exposed to mainstream cigarette smoke (CS) trapped in phosphate-buffered saline solutions (smoke-bubbled PBS). The protein was identified as heme oxygenase (HO) (heme, hydrogen donor:oxygen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.1.67
更新日期:1994-01-01 00:00:00
abstract::Plumbagin (PL) (5-hydroxy-2-methyl-1,4-napthoquinone), a medicinal plant-derived naphthoquinone, was isolated from the roots of the Plumbago zeylanica L. (also known as Chitrak). The roots of P. zeylanica L. have been used in Indian medicine for >2500 years as an anti-atherogenic, cardiotonic, hepatoprotective and neu...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr249
更新日期:2012-01-01 00:00:00
abstract::The role of sulfation in the metabolic activation of the liver carcinogen N-hydroxy-4'-fluoro-4-acetylaminobiphenyl (N-OH-FAABP) in male rat liver was investigated. N-OH-FAABP was a substrate for sulfotransferases in vitro and sulfation was inhibited by the sulfotransferase inhibitors pentachlorophenol (PCP) and 2,6-d...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.12.2285
更新日期:1989-12-01 00:00:00
abstract::Colorectal cancer is the second leading cause of cancer deaths worldwide with diet playing a prominent role in disease initiation and progression. Flavonoids are secondary plant compounds that are suggested as protective ingredients of a diet rich in fruits and vegetables. We here tested whether flavone, a flavonoid t...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm040
更新日期:2007-07-01 00:00:00
abstract::Modulation of cellular thiols has been used to ameliorate the toxic side effects associated with cancer chemotherapy and is currently being investigated as a novel therapeutic strategy in cancer treatment. One of the most extensively studied modulators of thiol levels is N-acetylcysteine (NAC), a cytoprotective drug w...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.9.2099
更新日期:1995-09-01 00:00:00
abstract::EphB2, a receptor tyrosine kinase regulated by the beta-catenin/Tcf4 complex, is expressed in the proliferative compartment of mouse intestine and regulates bidirectional migration of intestinal precursor cells in the crypt-villus axis through repulsive interaction with Ephrin-B ligands. Recently, it has been shown th...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi259
更新日期:2006-03-01 00:00:00
abstract::Treatment of SV40-infected CV-1 cells with the ultimate carcinogen anti-benzo[a]pyrene diol epoxide (BPDE) at 1 X 10(-4) mg/ml or higher reduced the rate of viral DNA synthesis to an extent dependent on the BPDE concentration; similar reductions in cellular DNA synthesis were produced in infected and uninfected cells....
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.2.125
更新日期:1983-01-01 00:00:00
abstract::In this study, we determined the wavelength dependence of u.v.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblasts. Pyrimidine dimers were quantified using the T4 endonuclease V assay, cell killing was measured as loss of colony forming ability and mutation inducti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.11.1829
更新日期:1986-11-01 00:00:00
abstract::Occupational nitrosamine exposures from a rubber vehicle seal (VS) curing operation were compared with the peripheral blood lymphocyte concentrations of two nitrosamine-related DNA adducts, N(7)-methylguanine (N(7)mdG) and O(6)-methylguanine (O(6)mdG), and with the activity of the enzyme that repairs O(6)mdG adducts, ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.1.29
更新日期:2000-01-01 00:00:00
abstract::The purpose of this work was to study the relative activities and stabilities of phase-I and phase-II drug metabolizing enzymes in incubation mixtures used in vitro genotoxicity testing in order to optimize the conditions of the assay, increase sensitivity and eliminate false negative results. Cytochrome P-450, NADPH-...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.9.1179
更新日期:1987-09-01 00:00:00
abstract::Iron exclusion has been used to identify foci of hepatocellular alteration (FHA) in rats exposed to known carcinogens. This study investigates whether basophilic FHA occurring spontaneously in aged female Fischer 344 rats also exclude iron. Female rats 514-544 days of age were given supplemental iron by injection for ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.12.2253
更新日期:1990-12-01 00:00:00
abstract::The resistant hepatocyte model was used to study expression of tumor-associated aldehyde dehydrogenase (ALDH) activity during the course of rat hepatocarcinogenesis. The hepatic ALDH phenotype was determined at intervals over 280 days by histochemical analysis, total ALDH activity assays and gel electrophoresis, using...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.12.1679
更新日期:1984-12-01 00:00:00
abstract::In this study we have investigated the relationship between the dose of 1,2-dimethylhydrazine (DMH) and the yield (and location) of tumours in a mouse strain susceptible to colon tumour induction. Female SWR mice were injected with 6.8 mg/kg DMH i.p. once a week for 1, 5, 10 and 20 weeks and the animals were followed ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.3.509
更新日期:1999-03-01 00:00:00
abstract::Inbred strains of mice with differential response to known tumor promoters were compared with respect to their susceptibility to modulation of hepatic antioxidant enzymes by long-term treatment with high fat diet (HF) and phenobarbital (PB). Mice of the C57BL/6J (C57), C3H/HeOuJ (C3H) and DBA/2J (DBA) strains were fed...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.6.1225
更新日期:1993-06-01 00:00:00
abstract::Using conventional alkaline sucrose sedimentation analysis, we have compared the initial yield and subsequent enzymatic repair of DNA damage induced in cultured human [normal (GM38 and GM43) and xeroderma pigmentosum (XP12BE)] fibroblasts by the monofunctional alkylating agent methylmethanesulfonate (MMS). Exposure of...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/9.12.2257
更新日期:1988-12-01 00:00:00
abstract::Germline genetic variability might contribute, at least partially, to the survival of pancreatic ductal adenocarcinoma (PDAC) patients. Two recently performed genome-wide association studies (GWAS) on PDAC overall survival (OS) suggested (P < 10(-5)) the association between 30 genomic regions and PDAC OS. With the aim...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgw080
更新日期:2016-10-01 00:00:00
abstract::To evaluate the significance of C-C chemokine receptor type 5 (CCR5) in lung tumor development, we compared carcinogen-induced tumor growth in CCR5 knockout (CCR5(-/-)) mice and wild-type (CCR5(+/+)) mice. CCR5(-/-) mice showed reduced urethane (1g/kg)-induced tumor incidence when compared with those of CCR5(+/+) mice...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs265
更新日期:2012-12-01 00:00:00
abstract::Several nitroarenes derived from benzo[a]pyrene and perylene and the parent hydrocarbons have been assayed for mutagenicity in the Salmonella microsome test and for affinity for the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-receptor protein in rat liver cytosol. 1- and 3-nitrobenzo[a]pyrene are mutagenic in the absen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.7.925
更新日期:1984-07-01 00:00:00
abstract::Alterations in the Rb pathway have been described in many different tumors. In order to study this cell cycle regulatory mechanism in murine T cell lymphomas, we have analyzed the RNA and protein expression of the cyclin D1, cdk4 and retinoblastoma genes in primary tumor samples. We have detected overexpression of the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.9.1675
更新日期:1999-09-01 00:00:00
abstract::2-methoxyestradiol (2-MeOE(2)) is an endogenous metabolite of 17beta-estradiol and a proposed inhibitor of tumor growth and angiogenesis. However, 2-MeOE(2) is also an inhibitor of microtubule assembly and other microtubule inhibitors, e.g. colcemid and diethylstilbestrol, induce aneuploidy and cell transformation in ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.4.735
更新日期:2000-04-01 00:00:00
abstract::DNA-demethylating agents activate tumor suppressor genes that are silenced by DNA methylation in cancer and are therefore emerging as a novel approach to cancer therapy. 5-azacytidine (VIDAZA), the first representative of this class of drugs was approved for treatment of myelodysplastic syndromes and is currently bein...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt284
更新日期:2014-01-01 00:00:00
abstract::The purpose of this study was to examine the activity and associated kinetic parameters of epidermal protein kinase C (PKC) following stimulation by sn-1,2-dioctanoylglycerol (DIC8) or 12-O-tetradecanoylphorbol-13-acetate (TPA) and to examine the relationship between levels of epidermal PKC activity and the induction ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.5.833
更新日期:1989-05-01 00:00:00
abstract::Chrysoidine dyes are used by fishermen to colour bait and recent epidemiological evidence has suggested that this may explain why they are at increased risk of urothelial cancer. In recent years Bismark brown dyes have been used as chrysoidine substitutes. Previous mutagenicity studies on these azo-dyes have examined ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.11.1921
更新日期:1986-11-01 00:00:00
abstract::To elucidate the function of the Ras-homologous GTPase Rac1 in hepatocarcinogenesis induced by diethylnitrosamine (DEN), mice lacking hepatic Rac1 expression were treated with DEN and compared to the wild-type (WT). Rac1 knock-out (KO) mice were found to have a lower tumor yield as compared to Rac1 proficient mice. Th...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgu323
更新日期:2015-03-01 00:00:00
abstract::Male F344 rats were administered phenobarbital, polychlorinated biphenyl (PCB), retinol acetate, indomethacin, 6-amino-caproic acid, dexamethasone (DEX) or diethylmaleate (DEM) for one week and then were treated with these chemicals plus butylated hydroxyanisole (BHA) for a further four weeks. Histopathologically, the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.10.1555
更新日期:1987-10-01 00:00:00
abstract::Changes in lipid metabolism were monitored in rat hepatocyte nodules at certain time points over 9 months. Tissue obtained from partially hepatectomized rats, collected over a period of 7 days, were included as a control for normal hepatocyte cell proliferation. Two important features regarding the lipid profiles of h...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.5.795
更新日期:2001-05-01 00:00:00
abstract::Comparative X-ray crystallographic structure analyses have been carried out on seven cyclopenta[a]phenanthrenes, namely 15,16-dihydocyclopenta[a]phenanthren-17-one and its 2-, 6- and 12-methyl homologues (non-carcinogens) and the 7-and 11-methyl and 1,11-methano derivatives (carcinogens). All-valence-electron molecula...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/4.12.1569
更新日期:1983-12-01 00:00:00