Mitomycin C-induced reduction of keratocytes and fibroblasts after photorefractive keratectomy.

abstract：PURPOSE:AIPL1 mutations cause the severe inherited blindness Leber congenital amaurosis (LCA). The similarity of AIPL1 to tetratricopeptide repeat (TPR) cochaperones that interact with the chaperone Hsp90 and the ability of AIPL1 to suppress the aggregation of NUB1 fragments in a chaperone-like manner suggest that AIPL...

journal_title：Investigative ophthalmology & visual science

authors： Hidalgo-de-Quintana J,Evans RJ,Cheetham ME,van der Spuy J

更新日期：2008-07-01 00:00:00

abstract：PURPOSE:To examine the involvement of p27(KIP1) in the regulation of the proliferation of the developing corneal endothelium. METHODS:Central and peripheral corneas in C57Bl6 mice at postnatal day (P)1, P11, and 12 weeks after birth were analyzed by immunocytochemistry with anti-p27(KIP1), -p57(KIP2), and -proliferati...

journal_title：Investigative ophthalmology & visual science

authors： Yoshida K,Kase S,Nakayama K,Nagahama H,Harada T,Ikeda H,Harada C,Imaki J,Ohgami K,Shiratori K,Ilieva IB,Ohno S,Nishi S,Nakayama KI

更新日期：2004-07-01 00:00:00

abstract：Purpose:Aging affects a variety of visual functions. In this study, we aim to quantitatively investigate the temporal characteristics of visual processing in aging. Methods:Twelve younger (24.1 ± 1.6 years) and 12 older observers (58.4 ± 3.6 years) participated in the study. All participants had normal or corrected-to...

journal_title：Investigative ophthalmology & visual science

authors： He X,Shen M,Cui R,Zheng H,Ruan X,Lu ZL,Hou F

更新日期：2020-05-11 00:00:00

abstract：PURPOSE:Lens epithelial tissue does not normally express major histocompatibility complex (MHC) class I molecules. In addition, the mechanism of self-tolerance to intraocular antigens is unknown. To study the effect of class I expression in the lens, transgenic mice were produced that express an allo-MHC class I molecu...

journal_title：Investigative ophthalmology & visual science

authors： Martin WD,Egan RM,Stevens JL,Woodward JG

更新日期：1995-05-01 00:00:00

abstract：PURPOSE:To study the survival of adult retinal grafts prepared in a physiologically optimized way. METHODS:Twenty-three rabbits received an adult full-thickness rabbit retinal transplant positioned under the host retina, using a vitrectomy technique. The transplants were prepared using a procedure based on a previousl...

journal_title：Investigative ophthalmology & visual science

authors： Wassélius J,Ghosh F

更新日期：2001-10-01 00:00:00

abstract：PURPOSE:To isolate and characterize bovine lens endopeptidase activity that cleaves the Phe-Ser bond in peptide substrates. METHODS:The protease activity in young bovine lens homogenate was measured using the Mca-(Ala(7),Lys(Dnp)(9))-bradykinin substrate. Degradation of bradykinin and other unlabeled peptide substrate...

journal_title：Investigative ophthalmology & visual science

authors： Chaerkady R,Sharma KK

更新日期：2004-04-01 00:00:00

abstract：PURPOSE:Contraction of fibroblasts and the resultant tractional force is a contributing factor to fibrotic diseases of the eye, such as proliferative vitreoretinopathy (PVR). Transforming growth factor (TGF)-beta is abundant in the eye, and is one of the growth factors thought to contribute to the development of PVR. A...

journal_title：Investigative ophthalmology & visual science

authors： Ikuno Y,Kazlauskas A

更新日期：2002-01-01 00:00:00

abstract：:Scar formation and fibrosis are the most common causes of ultimate glaucoma filtration surgery failure. The authors used Coulter counter, hexosaminidase, and 3H-thymidine assays to evaluate the effects of mithramycin, mitomycin, daunorubicin, and bleomycin on human subconjunctival fibroblast growth in tissue culture. ...

journal_title：Investigative ophthalmology & visual science

authors： Lee DA,Lee TC,Cortes AE,Kitada S

更新日期：1990-10-01 00:00:00

abstract：PURPOSE:Adeno-associated viral (AAV) vectors have been used to express several different proteins in the eye. The purpose of this study was to determine whether AAV-mediated intraocular gene transfer of pigment epithelium-derived factor (PEDF) inhibits the development of choroidal neovascularization (CNV) in a murine m...

journal_title：Investigative ophthalmology & visual science

authors： Mori K,Gehlbach P,Yamamoto S,Duh E,Zack DJ,Li Q,Berns KI,Raisler BJ,Hauswirth WW,Campochiaro PA

更新日期：2002-06-01 00:00:00

abstract：Purpose:Contrast agents applicable for optical coherence tomography (OCT) imaging are rare. The intrascleral aqueous drainage system would be a potential application for a contrast agent, because the aqueous veins are of small diameter and located deep inside the highly scattering sclera. We tested lipid emulsions (LEs...

journal_title：Investigative ophthalmology & visual science

authors： Gottschalk HM,Wecker T,Khattab MH,Fischer CV,Callizo J,Rehfeldt F,Lubjuhn R,Russmann C,Hoerauf H,van Oterendorp C

更新日期：2019-01-02 00:00:00

abstract：PURPOSE:Peroxynitrite generated during the early phase of experimental autoimmune uveoretinitis (EAU) causes peroxidation of docosahexaenoic acid (22:6), a principal unsaturated fatty acid of the photoreceptor membrane, to its hydroperoxide (22:6HP). During this phase, microglia migrate to the site of photoreceptors. T...

journal_title：Investigative ophthalmology & visual science

authors： Saraswathy S,Wu G,Rao NA

更新日期：2006-08-01 00:00:00

abstract：Purpose:We investigate morphologic and physiologic alterations of ganglion cells (GCs) in a streptozocin (STZ)-induced diabetic mouse model. Methods:Experiments were conducted in flat-mount retinas of mice 3 months after the induction of diabetes. Changes in morphology of four subtypes of GCs (ON-type RGA2 [ON-RGA2], ...

journal_title：Investigative ophthalmology & visual science

authors： Cui RZ,Wang L,Qiao SN,Wang YC,Wang X,Yuan F,Weng SJ,Yang XL,Zhong YM

更新日期：2019-04-01 00:00:00

abstract：Purpose:To quantify and evaluate macular superficial capillaries and large vessels separately using an optical coherence tomographic angiography (OCTA)-based automatic segmentation algorithm. Methods:In this cross-sectional study, all eyes were scanned using an OCTA device with 3 × 3 mm cube centered on the fovea. Ret...

journal_title：Investigative ophthalmology & visual science

authors： Lei J,Yi E,Suo Y,Chen C,Xu X,Ding W,Abdelfattah NS,Fan X,Lu H

更新日期：2018-04-01 00:00:00

abstract：PURPOSE:Recent evidence suggests that platelets play a major role in ischemia-reperfusion injury, not only through thrombus formation but also through participation in inflammatory reactions with leukocytes. This study was designed to investigate the contribution of platelets in leukocyte recruitment to inflamed region...

journal_title：Investigative ophthalmology & visual science

authors： Nishijima K,Kiryu J,Tsujikawa A,Miyamoto K,Honjo M,Tanihara H,Nonaka A,Yamashiro K,Katsuta H,Miyahara S,Honda Y,Ogura Y

更新日期：2004-03-01 00:00:00

abstract：PURPOSE:The purpose of the present study was to identify the expression of estrogen and progesterone receptor mRNA and of estrogen and progesterone receptor protein in the conjunctiva of healthy women. METHODS:Specimens of conjunctival tissue of 10 premenopausal women (age range, 13-38 years) were obtained during opht...

journal_title：Investigative ophthalmology & visual science

authors： Fuchsjäger-Mayrl G,Nepp J,Schneeberger C,Sator M,Dietrich W,Wedrich A,Huber J,Tschugguel W

更新日期：2002-09-01 00:00:00

abstract：:Monoclonal antibody to HSV glycoprotein D was derivatized with palmitic acid and incorporated into liposomes. These immunoliposomes bound specifically to intact mouse corneas infected with HSV-1 in vitro. Furthermore, in yield reduction assays, anti-gD immunoliposomes loaded with acyclovir proved far more effective at...

journal_title：Investigative ophthalmology & visual science

authors： Norley SG,Sendele D,Huang L,Rouse BT

更新日期：1987-03-01 00:00:00

abstract：PURPOSE:To estimate the heritability of the iridotrabecular angle width measured by anterior segment optical coherence tomography (ASOCT) in a classic twin study. METHODS:Twins aged 8 to 16 years were identified from the Guangzhou Twin Registry. ASOCT was used to obtain one horizontal scan, and the images were analyze...

journal_title：Investigative ophthalmology & visual science

authors： He M,Ge J,Wang D,Zhang J,Hewitt AW,Hur YM,Mackey DA,Foster PJ

更新日期：2008-04-01 00:00:00

abstract：Purpose:Global motion and global form perception have been found to be abnormal in the presence of amblyopia. How such deficits manifest in visual function reliant on the interactions between these two visual processing mechanisms has not been adequately explored. In the current study, we use dynamic Glass patterns (dG...

journal_title：Investigative ophthalmology & visual science

authors： Joshi MR,Simmers AJ,Jeon ST

更新日期：2020-05-11 00:00:00

abstract：PURPOSE:Extremely low gestational age neonates (ELGANs) requiring oxygen therapy often experience frequent episodes of intermittent hypoxia (IH) and are at high risk for severe retinopathy of prematurity (ROP). Using an established model for oxygen-induced retinopathy (OIR), we examined the hypothesis that there is a c...

journal_title：Investigative ophthalmology & visual science

authors： Beharry KD,Cai CL,Sharma P,Bronshtein V,Valencia GB,Lazzaro DR,Aranda JV

更新日期：2013-11-19 00:00:00

abstract：PURPOSE:To determine the effect of taxol and etoposide, hydrophobic drugs with antifibrosis activity, on the outcome of filtration surgery in glaucomatous monkeys. METHODS:Elevated intraocular pressure was produced bilaterally in eight cynomolgus monkeys by laser treatment of the trabecular meshwork. Four animals subc...

journal_title：Investigative ophthalmology & visual science

authors： Jampel HD,Thibault D,Leong KW,Uppal P,Quigley HA

更新日期：1993-10-01 00:00:00

abstract：PURPOSE:To characterize the response of cultured human retinal pigment epithelial (RPE) cells to lowered environmental oxygen. METHODS:The response of cultured RPE cells to lowered oxygen environments was compared to that of cell types of presumed high (Madin-Darby canine kidney [MDCK] cells, an epithelial cell line) ...

journal_title：Investigative ophthalmology & visual science

authors： Nash RW,McKay BS,Burke JM

更新日期：1994-05-01 00:00:00

abstract：:Experiments were performed with a model of focal, thermal-induced ulceration to test the clinical impression that vascularization prevents ulceration of the corneal stroma. Slow-release polymers containing a vasoproliferase agent (tumor angiogenesis factor) were placed in corneal pockets 2 mm central to the limbus of ...

journal_title：Investigative ophthalmology & visual science

authors： Conn H,Berman M,Kenyon K,Langer R,Gage J

更新日期：1980-04-01 00:00:00

abstract：Purpose:The purpose of this work was to test whether palisade endings express structural and molecular features of exocytotic machinery, and are associated with acetylcholine receptors, and enzymes for neurotransmitter breakdown. Methods:Extraocular rectus muscles from six cats were studied. Whole-mount preparations o...

journal_title：Investigative ophthalmology & visual science

authors： Blumer R,Streicher J,Carrero-Rojas G,Calvo PM,de la Cruz RR,Pastor AM

更新日期：2020-12-01 00:00:00

abstract：:Normal adult rabbit corneas were digested with 5% pepsin and their collagens extracted with acetic acid. Collagen extracts were fractionated by differential salt precipitation. The 2.5 M NaCl fraction was then redissolved with tris buffer and precipitated with sodium acetate. The precipitate contained a high-molecular...

journal_title：Investigative ophthalmology & visual science

authors： Cintron C,Hong BS

更新日期：1988-05-01 00:00:00

abstract：PURPOSE:The purpose of this study was to elucidate the differentiated expression patterns and phagocytic activities of two subtypes of microglia. METHODS:A rat optic nerve crush model was used to identify the expression patterns of two subtypes of microglia. Primary microglia were isolated from rat mixed glial culture...

journal_title：Investigative ophthalmology & visual science

authors： Haga A,Takahashi E,Inomata Y,Kawahara K,Tanihara H

更新日期：2016-05-01 00:00:00

abstract：Purpose:The purpose of this study was to describe the cellular architecture of normal human peripapillary sclera (PPS) and evaluate surface topography's role in fibroblast behavior. Methods:PPS cryosections from nonglaucomatous eyes were labelled for nuclei, fibrillar actin (FA), and alpha smooth muscle actin (αSMA) a...

journal_title：Investigative ophthalmology & visual science

authors： Szeto J,Chow A,McCrea L,Mozzer A,Nguyen TD,Quigley HA,Pitha I

更新日期：2021-01-04 00:00:00

abstract：Purpose:To define the functional roles of Grk1 and Grk7 in zebrafish cones in vivo. Methods:Genome editing was used to generate grk7a and grk1b knockout zebrafish. Electroretinogram (ERG) analyses of the isolated cone mass receptor potential and the b-wave were performed in dark-adapted zebrafish using a paired flash ...

journal_title：Investigative ophthalmology & visual science

authors： Chrispell JD,Dong E,Osawa S,Liu J,Cameron DJ,Weiss ER

更新日期：2018-10-01 00:00:00

abstract：PURPOSE:To determine the relationship between optical coherence tomography (OCT) images of the retina and retinal substructure in vitro and in vivo. METHODS:In vitro, OCT images of human and bovine retina were acquired after sequential excimer laser ablation of the inner retinal layers. Measurements of bands in the OC...

journal_title：Investigative ophthalmology & visual science

authors： Chauhan DS,Marshall J

更新日期：1999-09-01 00:00:00

abstract：:Autophagy and macropinocytosis are processes that are vital for cellular homeostasis, and help cells respond to stress and take up large amounts of material, respectively. The limbal and corneal epithelia have the machinery necessary to carry out both processes; however, autophagy and macropinocytosis are relatively u...

journal_title：Investigative ophthalmology & visual science

authors： Peng H,Park JK,Lavker RM

更新日期：2017-01-01 00:00:00

abstract：PURPOSE:To observe the spatial distribution of households with high loads of ocular chlamydia infection in children, before and after mass treatment with azithromycin to determine whether there exists spatial clustering of households with high loads of infection and the spatial scale of the clustering. METHODS:All res...

journal_title：Investigative ophthalmology & visual science

authors： Broman AT,Shum K,Munoz B,Duncan DD,West SK

更新日期：2006-01-01 00:00:00