Abstract:
:Myeloid dendritic cells (MyDCs), prime stimulators of antigen-specific immunity, can serve as one of the major reservoirs for human immunodeficiency virus type-1 (HIV-1). Utilizing mature monocyte-derived MyDCs generated with granulocyte/macrophage colony-stimulating factor, interleukin-4, and tumour necrosis factor-alpha as an in vitro model, we here present the first proof of concept for liposomal compound delivery to these cells by specifically addressing CD209, i.e. DC-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN), a MyDC-associated C-type lectin implicated in the transmission of HIV-1 to T helper cells. By employing a liposomally entrapped tracer, calcein, we demonstrate by flow cytometry and mathematics a superior targeting efficacy for DC-SIGN, as compared with select other MyDC markers (CD1a, CD4, CD45R0, and CD83). Fluorescence microscopy reveals time-dependent surface binding and intracellular uptake of DC-SIGN-specific liposomes by both immature and mature MyDCs. This pilot study implies that liposomal targeting to CD209 and related C-type lectins may afford therapeutic intracellular drug delivery to MyDCs and other reservoir and nonreservoir cells susceptible to infection with HIV-1.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
Gieseler RK,Marquitan G,Hahn MJ,Perdon LA,Driessen WH,Sullivan SM,Scolaro MJdoi
10.1111/j.0300-9475.2004.01431.xkeywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
415-24issue
5eissn
0300-9475issn
1365-3083pii
SJI1431journal_volume
59pub_type
杂志文章abstract::The ability of different subpopulations of blood mononuclear cells to serve as accessory cells in the activation of CD4+ and CD8+ T cells via Ti-CD3 or with phytohaemagglutinin (PHA) was studied. Pure CD4+ or CD8+ T cells did not respond to particle-bound anti-CD3 monoclonal antibodies (MoAb) or PHA, whereas responses...
journal_title:Scandinavian journal of immunology
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doi:10.1111/j.1365-3083.1988.tb02383.x
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journal_title:Scandinavian journal of immunology
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doi:10.1111/j.1365-3083.1976.tb03856.x
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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doi:10.1111/j.1365-3083.1980.tb00040.x
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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journal_title:Scandinavian journal of immunology
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