Abstract:
:Malignant tissue samples may sometimes be the only source of biological material for forensic investigations, including identification of individuals or paternity testing. However, in use of such samples, uncertainties due to microsatellite instability (MSI) and loss of heterozygosity (LOH) often associated with neoplasias may be encountered. In this study, we have analysed the applicability of autosomal tetranucleotide short tandem repeat (STR) markers, which are routinely used in forensic analysis, to gain genetic information. MSI and LOH were analysed in 41 surgically removed gastrointestinal cancer specimens and the adjascent non-cancerous tissue marginals. The cancer specimens showed great variability in their genetic phenotypes due to MSI or LOH, with only 32% being microsatellite-stable. Of the 15 autosomal STR loci analysed, only TH01 had no MSI-type alteration in these samples. The loci most frequently affected by MSI were D8S1179, D21S11, D18S51 and D19S433 (MSI in 15-17% of cases). LOH-type alterations were observed at all of the loci, including the amelogenin locus used for sex determination. The highest LOH frequency was found at locus D18S51 (27%). The genetic alterations at the marker loci may indicate false homozygosity or heterozygosity, and false gender may result from erroneous deduction of DNA profiles. Therefore, typing of autosomal STRs from malignant tissues in forensic settings warrants careful interpretation of MSI and LOH results together with microscopic analysis of a tissue specimen. Results by two commercially available and widely used forensic DNA profiling kits used here were comparable.
journal_name
Forensic Sci Intjournal_title
Forensic science internationalauthors
Vauhkonen H,Hedman M,Vauhkonen M,Kataja M,Sipponen P,Sajantila Adoi
10.1016/j.forsciint.2003.10.016keywords:
subject
Has Abstractpub_date
2004-01-28 00:00:00pages
159-67issue
2-3eissn
0379-0738issn
1872-6283pii
S037907380300478Xjournal_volume
139pub_type
杂志文章abstract::During a stabbing, apparel fabrics are usually damaged and may be penetrated. Despite numerous studies considering forces required to penetrate skin and human stabbing performance, none have systematically evaluated which variables affect severance appearance from a textile science perspective using a human stabbing p...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.01.024
更新日期:2015-04-01 00:00:00
abstract::Preliminary results concerning the use of liquid crystals (LC) in fingerprinting are given. These were based on thin layers of liquid crystals, changing colours (observed in linear polarized light) due to dissolved organic matter, transferred from fingers' surfaces. The possibility is shown of using LC to illustrate n...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(91)90137-8
更新日期:1991-07-01 00:00:00
abstract::This study explores the applicability of micro-Raman spectroscopy as a non-destructive technique for the analysis of color toner printed counterfeits. The main aim of the research paper was to find out whether Raman spectroscopy is a suitable method for establishing the connection between different specimens of counte...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.10.007
更新日期:2012-11-30 00:00:00
abstract::Numerous studies have mentioned to the high percentage of violent deaths in prison psychiatric hospitals, with suicide being the principal cause. The aim of this study was to analyze the circumstances related with the deaths recorded in a high security institution. Postmortem reports on all the deaths at the Alicante ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(00)00441-2
更新日期:2001-07-15 00:00:00
abstract::Modifications of cotton and polyester textiles due to shots fired at short range were analyzed with a variable pressure scanning electron microscope (VP-SEM). Different mechanisms of fiber rupture as a function of fiber type and shooting distance were detected, namely fusing, melting, scorching, and mechanical breakag...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2016.12.033
更新日期:2017-03-01 00:00:00
abstract:AIM:To validate a method for determining equivalent drug cutoff concentrations for tetrahydrocannabinol and amphetamine in blood and oral fluid, which ensures that the drug prevalence in samples of blood and oral fluid taken simultaneously is equal. METHODS:A method using regression analysis of drug concentrations for...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.06.024
更新日期:2011-10-10 00:00:00
abstract::Pyrethroids, organic compounds similar to natural pyrethrums, constitute the majority of insecticides. Pyrethroids are widely used around the world owing to their excellent selective toxicity to certain insects. In addition, they are easily found in daily life, accounting for most household pesticides. Owing to the ea...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.06.004
更新日期:2019-09-01 00:00:00
abstract::Two pig (Sus scrofta L.) carcasses were placed in sunlit and shaded plots in September 2003, and again in May 2004. Mean ambient temperatures between sunlit and shaded plots were not significantly different in either September or May, but mean ambient temperatures at sunlit and shaded plots in 2004 were significantly ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.01.008
更新日期:2006-12-20 00:00:00
abstract::Haplotype frequencies of eight Y-chromosome STR loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393 and DYS385) were determined from a sample of 285 unrelated males from Canary Islands. ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2004.05.004
更新日期:2005-03-10 00:00:00
abstract::The population of Curaçao, Netherlands Antilles (133,000) shows a very high prevalence of end-stage renal disease (approximately 1 per 1,000). These patients are often treated chronically with haemodialysis. As the drinking water on the island is prepared by distillation of sea water, the haemodialysis fluid used to b...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00159-7
更新日期:2002-08-14 00:00:00
abstract::Foreign body aspiration is frequently encountered in young children, in the majority of cases it will not lead to an adverse outcome. However, in case of coin battery ingestion more serious adverse outcomes, including death, have been reported. We present a case with fatal outcome due to exsanguination, of a 2-year-ol...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2010.02.001
更新日期:2010-05-20 00:00:00
abstract::Organised criminality is a great concern for national/international security. The demonstration of complex crimes is increasingly dependant on knowledge distributed within law-enforcement agencies and scientific disciplines. This separation of knowledge creates difficulties in reconstructing and prosecuting such crime...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.06.032
更新日期:2007-04-11 00:00:00
abstract::The discrimination of sex is central to the process of establishing a personal identification from human skeletal remains. Previous study has demonstrated that osteometric analysis of the sternal extremity of the fourth rib provides a reasonably accurate method for assessing this biological attribute in diverse human ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.09.007
更新日期:2012-11-30 00:00:00
abstract::Allele frequencies, forensic parameters for the 11 STR loci (TH01, TPOX, CSF1PO, D13S317, D7S820, D16S539, VWA, FESFPS, F13A01, F13B and LPL) were determined in a sample of 140-1262 unrelated adults from the Upper Silesia region (Poland). Comparison of allele frequencies for examined STR loci between the Upper Silesia...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.01.011
更新日期:2007-05-03 00:00:00
abstract::The article is devoted to the comparative analysis of effectiveness of the most widely used methods for medico-legal examination of the hyoid bone and laryngeal cartilages, i.e. palpation, radiography and complete preparation. A consecutive study of 137 cases of hanging (including 20 cases of roentgenological analysis...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(97)00101-1
更新日期:1997-08-22 00:00:00
abstract::One hundred and seventy nine cases of acute poisoning constituting 1.1% of all admissions to the medical unit over 3 years were analysed. Eighty-three per cent of the patients were under 30 years of age and 72% were males. The common agents were: OP insecticides in 51%, kerosene oil in 12%, rat poison in 8% and drugs ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(88)90221-6
更新日期:1988-01-01 00:00:00
abstract::Fifteen autosomal short tandem repeat (STR) markers (D3S1358, HUMTH01, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1PO, PENTA D, HUMvWA, D8S1179, HUMTPOX, FGA) were analyzed in 1734 individuals living in urban areas of cities from six different Argentinian provinces (Buenos Aires, Neuquén, Tucumán, L...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2006.02.042
更新日期:2007-01-05 00:00:00
abstract::When tried in court, mothers accused of neonaticide may claim that the umbilical cord just broke during birth and the newborn child bled to death accordingly. To evaluate the possibility of a breakage of the umbilical cord is the goal of this work. Therefore 25 umbilical cords from neonates of both sexes born at term ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2013.12.002
更新日期:2014-03-01 00:00:00
abstract::Though the child abuse syndrome is usually considered to have been first described in the middle of the present century, references to child abuse occur throughout history and in the 19th century, detailed descriptions were published in the French medical literature. ...
journal_title:Forensic science international
pub_type: 历史文章,杂志文章
doi:10.1016/0379-0738(86)90008-3
更新日期:1986-02-01 00:00:00
abstract::Human decomposition is a complex biological process driven by an array of variables which are not clearly understood. The medico-legal community have long been searching for a reliable method to establish the post-mortem interval (PMI) for those whose deaths have either been hidden, or gone un-noticed. To date, attemp...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.05.018
更新日期:2015-08-01 00:00:00
abstract::Technology provides new ways to access customers and suppliers while enhancing the security of off-line criminal activity. Since the first cryptomarket, Silk Road, in 2011, cryptomarkets have transformed the traditional drug sale by facilitating the creation of a global network of vendors and buyers. Due to the fragme...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2018.05.002
更新日期:2018-07-01 00:00:00
abstract::Forensic anthropology makes an important contribution to human identification and assessment of the causes and mechanisms of death and body disposal in criminal and civil investigations, including those related to atrocity, disaster and trafficking victim identification. The methods used are comparative, relying on as...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2017.08.015
更新日期:2017-10-01 00:00:00
abstract::The hairy maggot blow fly, Chrysomya rufifacies (Diptera: Calliphoridae), is a forensically important fly often encountered on human and other vertebrate remains in temperate and tropic regions throughout the world including Australia, Asia, Central America and North America. C. rufifacies was reared under controlled ...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2014.09.023
更新日期:2014-12-01 00:00:00
abstract::The present study was made on an experimental animal model of a death from anaphylaxis, in which postmortem changes in levels of histamine and 1-methylhistamine, in whole blood were measured. Instead of the usual immunological method administering compound 48/80, a degranulating agent of mast cell and the effect close...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/0379-0738(90)90233-o
更新日期:1990-03-01 00:00:00
abstract::Anabolic androgenic steroids (AAS) are the main class of doping agents and their consumption produces adverse effects involving several organs and systems. Three cases of sudden cardiac death (SCD) and one of death due to congestive heart failure of previously healthy athletes who were AAS users are herein reported. C...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.10.032
更新日期:2012-04-10 00:00:00
abstract::Cocaine abuse is widespread all over the world, and is performed generally by sniffing, injecting or smoking cocaine or crack. The distinction between the recreational use of cocaine from the practice of the so called "coqueo" is still an issue in those countries where this habit is diffused and where it is not consid...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2012.09.005
更新日期:2013-04-10 00:00:00
abstract::Trends in forensic toxicology show the advancement of rapid and sensitive analytical methods for qualitative and quantitative analysis of drugs of abuse. However, forensic toxicologists are continuously faced with the challenges of identifying and quantifying drug blood concentration while simultaneously struggling wi...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2019.03.048
更新日期:2019-07-01 00:00:00
abstract::In the case of disasters or crime scenes, forensic anthropometric methods have been utilized as a reliable way to quickly confirm the identification of victims using only a few parts of the body. A total of 321 measurement data (from 167 males and 154 females) were analyzed to investigate the suitability of detailed h...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2015.10.014
更新日期:2015-12-01 00:00:00
abstract::The authors have proposed a quantitative method for assessing weight of evidence in the case where a fingermark from a crime scene is compared with a set of control prints from the ten fingers of a suspect. The approach is based on the notion of calculating a Likelihood Ratio (LR) that addresses a pair of propositions...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/j.forsciint.2011.08.008
更新日期:2012-01-10 00:00:00
abstract::The purpose of the study was to test the potential utility of a geographical profiling approach for three separate series of rapes committed by a single offender. Two different mathematical distance-decay functions using either a normal distribution with a mean distance and standard deviation based on previous researc...
journal_title:Forensic science international
pub_type: 杂志文章
doi:10.1016/s0379-0738(02)00385-7
更新日期:2003-01-09 00:00:00