Abstract:
:Previous studies have raised the possibility that a decrease in voltage-gated K+ currents may contribute to hyperexcitability of injured dorsal root ganglion (DRG) neurons and the emergence of neuropathic pain. We examined the effects of axotomy on mRNA levels for various Kv1 family subunits and voltage-gated K+ currents in L4-L5 DRG neurons from sham-operated and sciatic nerve-transected rats. RNase protection assay revealed that Kv1.1 and Kv 1.2 mRNAs are highly abundant while Kv1.3, Kv1.4, Kv1.5 and Kv1.6 mRNAs were detected at lower levels in L4-L5 DRGs from sham and intact rats. Axotomy significantly decreased Kv1.1, Kv1.2, Kv1.3 and Kv1.4 mRNA levels by approximately 35%, approximately 60%, approximately 40% and approximately 80%, respectively, but did not significantly change Kv1.5 or Kv1.6 mRNA levels. Patch clamp recordings revealed two types of K+ currents in small-sized L4-L5 DRG neurons: sustained delayed rectifier currents elicited from a -40 mV holding potential and slowly inactivating A-type currents that was additionally activated from a -120 mV holding potential. Axotomy decreased both types of K+ currents by 50-60% in injured DRG neurons. In addition, axotomy increased the alpha-dendrotoxin sensitivity of the delayed rectifier, but not slow A-type K+ currents in injured DRG neurons. These results suggest that Kv1.1 and Kv1.2 subunits are major components of voltage-gated K+ channels in L4-L5 DRG neurons and that the decreased expression of Kv1-family subunits significantly contributes to the reduction and altered kinetics of Kv current in axotomized neurons.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Yang EK,Takimoto K,Hayashi Y,de Groat WC,Yoshimura Ndoi
10.1016/j.neuroscience.2003.11.014keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
867-74issue
4eissn
0306-4522issn
1873-7544pii
S0306452203008741journal_volume
123pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract::The peculiar shape and disposition of Purkinje cell (PC) dendrites, planar and highly branched, offers an optimal model to analyze cellular and molecular regulators for the acquisition of neuronal dendritic trees. During the first 2 weeks after the end of the proliferation period, PCs undergo a 2-phase remodeling proc...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2008.12.035
更新日期:2009-09-01 00:00:00
abstract::We have investigated a potential mechanism by which a diet, similar in composition to the typical diet of most industrialized western societies rich in saturated fat and refined sugar (HFS), can influence brain structure and function via regulation of neurotrophins. We show that animals that learn a spatial memory tas...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00123-9
更新日期:2002-01-01 00:00:00
abstract::We have investigated effects of letrozole, an aromatase inhibitor, on spatial learning and memory, expression of neural cell adhesion molecules (NCAM) and catecholaminergic neurotransmitters in the hippocampus and cortex of female rats. In the intact model, adult Sprague-Dawley rats were divided into four groups (n=8)...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.09.005
更新日期:2008-01-02 00:00:00
abstract::With the six convulsants studied (Soman, intrahippocampal penicillin, bicuculline, pentylenetetrazol, picrotoxin and strychnine), the anatomical distribution of changes in local cerebral glucose utilization was related to the type of seizure observed. Strychnine induced a few very intense motor convulsions during the ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(85)90146-0
更新日期:1985-03-01 00:00:00
abstract::It has long been known that storage of information in working memory suffers as a function of proactive interference. Here we review the results of experiments using approaches from cognitive neuroscience to reveal a pattern of brain activity that is a signature of proactive interference. Many of these results derive ...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2005.06.042
更新日期:2006-04-28 00:00:00
abstract::Clinical reports show that oxytocin (OT) is related to stress-related disorders such as depression, anxiety disorder, and post-traumatic stress disorder. Two key structures in the brain should be paid special attention with regard to stress regulation, namely, the hypothalamus and the hippocampus. The former is the re...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2019.07.046
更新日期:2019-10-01 00:00:00
abstract::We have recently reported on the efficacy of an N-methyl-d-aspartate (NMDA) receptor partial antagonist, S-Methyl-N,N-diethylthiolcarbamate sulfoxide (DETC-MeSO), in improving outcome following stroke, including reduced infarct size and calcium influx, suppressing the endoplasmic reticulum (ER) stress-induced apoptosi...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.05.042
更新日期:2015-08-06 00:00:00
abstract::Adolescence is a time of continued brain maturation, particularly in limbic and cortical regions, which undoubtedly plays a role in the physiological and emotional changes coincident with adolescence. An emerging line of research has indicated that stressors experienced during this crucial developmental stage may affe...
journal_title:Neuroscience
pub_type: 杂志文章,评审
doi:10.1016/j.neuroscience.2012.10.048
更新日期:2013-09-26 00:00:00
abstract::Extinction reflects a decrease in the conditioned response (CR) following non-reinforcement of a conditioned stimulus. Behavioral evidence indicates that extinction involves an inhibitory learning mechanism in which the extinguished CR reappears with presentation of an unconditioned stimulus. However, recent studies o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2010.08.066
更新日期:2010-11-24 00:00:00
abstract::We have previously shown that the spinal cord is capable of learning a sensorimotor task in the absence of supraspinal input. Given the action of brain-derived neurotrophic factor (BDNF) on hippocampal learning, the current studies examined the role of BDNF in spinal learning. BDNF is a strong synaptic facilitator and...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2007.05.051
更新日期:2007-09-21 00:00:00
abstract::Deep brain stimulation of the anterior nucleus of the thalamus has been proposed as novel therapy to treat intractable epilepsy. To optimize this approach, we proposed to study the involvement of this nucleus in a non-human primate model of mesial temporal lobe seizure. Two macaques were implanted with one chronic ele...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2019.06.018
更新日期:2019-10-15 00:00:00
abstract::Numerous epidemiological studies have shown an association between pesticide exposure and the increased risk of developing Parkinson's disease. Previously we have reported that Dichlorvos exposure can induce oxidative stress, resulting in over-expression of pro-apoptotic genes and finally caspase-dependent nigrostriat...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2013.11.004
更新日期:2014-01-31 00:00:00
abstract::Our human visual system exploits spatiotemporal regularity to interpret incoming visual signals. With a dynamic stimulus sequence of four collinear bars (predictors) appearing consecutively toward the fovea, followed by a target bar with varying contrasts, we have previously found that this predictable spatiotemporal ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.01.027
更新日期:2014-03-28 00:00:00
abstract::Cell surface glycoconjugates are thought to mediate cell-cell recognition and play roles in neuronal development and functions. We demonstrated here that exposure of neuronal cells to nanomolar levels of gangliosides Neu5Acalpha 8Neu5Acalpha 3Galbeta 4GlcCer, Galbeta 3GalNAcbeta 4(Neu5Acalpha 8Neu5Acalpha 3)Galbeta 4G...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(03)00259-8
更新日期:2003-01-01 00:00:00
abstract::The mutant mice tottering, leaner and the compound heterozygous tottering/leaner exhibit varying degrees of several abnormal neurological phenotypes including petit mal-like epilepsy, ataxia and an intermittent myoclonus-like movement disorder. Aberrant expression of tyrosine hydroxylase in cerebellar Purkinje cells o...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(95)00444-0
更新日期:1996-03-01 00:00:00
abstract::This study compared the pharmacology of adrenergic agents that influence plasma extravasation in normal animals with those agents that influence tissue injury in an inflammatory disease model. Specifically we studied the effects of beta 2- and alpha 2-adrenergic receptor agonists and antagonists on bradykinin-induced ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(91)90184-p
更新日期:1991-01-01 00:00:00
abstract::Emerging evidence indicates that the expression of trophic factors in the brain is regulated in an activity-dependent manner, which suggests an involvement of trophic factors in events controlled by input activity. We have investigated the possibility that visual sensory input impacts the expression of basic fibroblas...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(98)00243-7
更新日期:1999-01-01 00:00:00
abstract::Studies demonstrated that chronic high-dose homocysteine administration induced learning and memory impairment in animals. Atractylenolide III (Aen-III), a neuroprotective constituent of Atractylodis macrocephalae Koidz, was isolated in our previous study. In this study, we investigated potential benefits of Aen-III i...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2015.01.060
更新日期:2015-04-02 00:00:00
abstract::Pathological changes occur in areas of CNS tissue remote from inflammatory lesions in multiple sclerosis (MS) and its animal model experimental allergic encephalomyelitis (EAE). To determine if oxidative stress is a significant contributor to this non-inflammatory pathology, cortex tissues from mice with clinical sign...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2011.04.041
更新日期:2011-06-30 00:00:00
abstract::Homer is a postsynaptic density (PSD) scaffold protein that is involved in synaptic plasticity, calcium signaling and neurological disorders. Here, we use pre-embedding immunogold electron microscopy to illustrate the differential localization of three Homer gene products (Homer 1, 2, and 3) in different regions of th...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2014.01.066
更新日期:2014-04-25 00:00:00
abstract::Stimulant-induced exocytosis has been demonstrated in sympathetic ganglia of the rat by in vitro incubation of excised ganglia in the presence of tannic acid, which stabilizes vesicle cores after their exocytotic release. Sites of exocytosis were observed along non-synaptic regions of the surfaces of neuron somata and...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(96)00664-1
更新日期:1997-10-01 00:00:00
abstract::Extracellular signal-regulated kinases and activator protein 1 transcription factor have been functionally linked to addiction. It has also been shown that extracellular signal-regulated kinase activation can regulate cocaine-induced expression of c-Fos and FosB, two possible components of activator protein 1. A direc...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2005.09.001
更新日期:2006-01-01 00:00:00
abstract::Methyl-CpG-binding protein 2 is a characteristic member of the methyl-CpG-binding protein family of transcription regulators. In conjunction with Sin3, MeCP2 recruits class I histone deacetylases to methyl-CpG regions to suppress transcription. Rett syndrome, a disorder characterized by mental retardation and autistic...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(02)00586-9
更新日期:2003-01-01 00:00:00
abstract::Intracellular recordings were made from neurons in a rat locus coeruleus slice preparation in vitro. A postsynaptic potential was evoked by electrical stimulation of afferents to the neurons. CI-977 ([5R-(5a,7a,8b)]-N-methyl-N-[7-(1-pyrrolidinyl)-1-oxaspiro[4.5]dec -8-yl[-4-benzofuranacetamide monohydrochloride) cause...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(92)90123-j
更新日期:1992-01-01 00:00:00
abstract::Acid-sensing ion channels (ASICs) regulate synaptic activities and play important roles in neurodegenerative diseases. They are highly expressed in the striatum, where medium spiny neurons (MSNs) are a major population. Given that the properties of ASICs in MSNs are unknown, in this study, we characterized ASICs in MS...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2009.04.029
更新日期:2009-08-04 00:00:00
abstract::The widespread distribution of neurons containing alpha-atrial natriuretic polypeptide-like immunoreactivity in the rat brain was demonstrated using radioimmunoassay and immunohistochemistry in conjunction with specific antisera. The highest concentrations of alpha-atrial natriuretic polypeptide-like immunoreactivity ...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/0306-4522(85)90190-3
更新日期:1985-11-01 00:00:00
abstract::Since functional imaging of whole body movements is not feasible with functional magnetic resonance imaging (fMRI), the present study presents in vivo functional near-infrared spectroscopy (fNIRS) as a suitable technique to measure body movement effects on fronto-temporo-parietal cortical activation in single- and dua...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2016.11.032
更新日期:2017-02-20 00:00:00
abstract::Arc (also termed activity-regulated cytoskeleton-associated protein or Arg3.1), is an effector immediate early gene whose upregulation has been demonstrated during events of synaptic plasticity. In the present study, the possibility that Arc would be specifically upregulated in rats during the acquisition of a quickly...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(01)00605-4
更新日期:2002-01-01 00:00:00
abstract::High resolution immunogold cytochemistry was used to investigate the subcellular distribution of protein kinase C gamma and delta in Purkinje cells of the rat cerebellum. Postembedding incubation with an antibody raised to a peptide sequence near the C-terminus of protein kinase C gamma resulted in strong labelling al...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/s0306-4522(97)00305-9
更新日期:1998-02-01 00:00:00
abstract::The pathological significance of intracellular Abeta accumulation in vivo is not yet fully understood. To address this, we have studied transgenic rats expressing Alzheimer's-related transgenes that accumulate Abeta intraneuronally in the cerebral and hippocampal cortices but do not develop extracellular amyloid plaqu...
journal_title:Neuroscience
pub_type: 杂志文章
doi:10.1016/j.neuroscience.2004.07.036
更新日期:2004-01-01 00:00:00