Trk is a calmodulin-binding protein: implications for receptor processing.


:The tyrosine kinase receptors for the neurotrophins (Trk) are a family of transmembrane receptors that regulate the differentiation and survival of different neuronal populations. Neurotrophin binding to Trk leads to the activation of several signalling pathways including a rapid, but moderate, increase in intracellular calcium levels. We have previously described the role of calcium and its sensor protein, calmodulin, in Trk-activated intracellular pathways. Here we demonstrate that calmodulin is able to precipitate TrkA from PC12 cell lysates. Using recombinant GST-fusion proteins containing the complete intracellular domain of TrkA, or fragments of this region, we show that calmodulin binds directly to the C-terminal domain of TrkA in a Ca2+-dependent manner. We have also co-immunoprecipitated endogenous Trk and calmodulin in primary cultures of cortical neurones. Moreover, we provide evidence that calmodulin is involved in the regulation of TrkA processing in PC12 cells. Calmodulin inhibition results in the generation of a TrkA-derived p41 fragment from the cytosolic portion of the protein. This fragment is autophosphorylated in tyrosines and can recruit PLCgamma and Shc adaptor proteins. These results suggest that calmodulin binding to Trk may be important for the regulation of Trk intracellular localization and cleavage.


J Neurochem


Llovera M,de Pablo Y,Egea J,Encinas M,Peiró S,Martín-Zanca D,Rocamora N,Comella JX





Has Abstract


2004-01-01 00:00:00














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    abstract::Oligodendroglial cell death in the myelin proteolipid protein (PLP) mutants can be partially rescued by the environment factor(s) supplied by the wild-type cells in vivo and in vitro. It is possible that the presence of PLP or DM-20 results in secretion of a factor or factors in the CNS influencing oligodendrocyte dev...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Nakao J,Yamada M,Kagawa T,Kim SU,Miyao Y,Shimizu K,Mikoshiba K,Ikenaka K

    更新日期:1995-06-01 00:00:00

  • Neonatal 6-hydroxydopamine and adult SKF 38393 treatments alter dopamine D1 receptor mRNA levels: absence of other neurochemical associations with the enhanced behavioral responses of lesioned rats.

    abstract::To study potential biochemical correlates of dopamine (DA) and serotonin receptor supersensitivity, rats were lesioned at 3 days after birth with 6-hydroxydopamine (6-OHDA; 67 micrograms in each lateral ventricle; desipramine pretreatment, 20 mg/kg i.p., 1 h) and then sensitized with the DA D1 agonist, SKF 38393 HCl (...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Gong L,Kostrzewa RM,Li C

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  • Steroid hormones block amyloid fibril-induced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) formazan exocytosis: relationship to neurotoxicity.

    abstract::Perhaps the most reproducible early event induced by the interaction of amyloid beta peptide (A beta) with the cell is the inhibition of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. We recently demonstrated that cytotoxic amyloid peptides such as A beta and human amylin inhibi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Liu Y,Schubert D

    更新日期:1998-12-01 00:00:00

  • Peripheral adipose tissue insulin resistance alters lipid composition and function of hippocampal synapses.

    abstract::Compelling evidence indicates that type 2 diabetes mellitus, insulin resistance (IR), and metabolic syndrome are often accompanied by cognitive impairment. However, the mechanistic link between these metabolic abnormalities and CNS dysfunction requires further investigations. Here, we evaluated whether adipose tissue ...

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    pub_type: 杂志文章


    authors: Sallam HS,Tumurbaatar B,Zhang WR,Tuvdendorj D,Chandalia M,Tempia F,Laezza F,Taglialatela G,Abate N

    更新日期:2015-04-01 00:00:00

  • Modulation of receptors for thyrotropin-releasing hormone by benzodiazepines: brain regional differences.

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    journal_title:Journal of neurochemistry

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    authors: Sharif NA,Burt DR

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  • Nerve growth factor regulates the expression of the cholinergic locus and the high-affinity choline transporter via the Akt/PKB signaling pathway.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Madziar B,Shah S,Brock M,Burke R,Lopez-Coviella I,Nickel AC,Cakal EB,Blusztajn JK,Berse B

    更新日期:2008-12-01 00:00:00

  • Neuro-inflammation induced in the hippocampus of 'binge drinking' rats may be mediated by elevated extracellular glutamate content.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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  • Activation of the extracellular signal-regulated kinase pathway contributes to the behavioral deficit of fragile x-syndrome.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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    更新日期:2012-05-01 00:00:00

  • The peripheral-type benzodiazepine receptor is present in astrocytes but is not a primary site of action for convulsants/anticonvulsants.

    abstract::High-affinity binding sites for [3H]PK 11195 and [3H]Ro 5-4864 with the properties of the peripheral-type benzodiazepine receptor were detected in primary cultures of both mouse neocortical and cerebellar astrocytes. The binding sites were enriched in mitochondrial fractions on differential centrifugation. An 18-kDa p...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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  • Deficiency of G3BP1, the stress granules assembly factor, results in abnormal synaptic plasticity and calcium homeostasis in neurons.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Martin S,Zekri L,Metz A,Maurice T,Chebli K,Vignes M,Tazi J

    更新日期:2013-04-01 00:00:00

  • The neuropathogenic contributions of lysosomal dysfunction.

    abstract::Multiple lines of evidence implicate lysosomes in a variety of pathogenic events that produce neurodegeneration. Genetic mutations that cause specific enzyme deficiencies account for more than 40 lysosomal storage disorders. These mostly pre-adult diseases are associated with abnormal brain development and mental reta...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


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    abstract::Mitogen-activated protein (MAP) kinases have been implicated in multiple responses to extracellular stimuli. In this study we show that MAP kinase activity is enhanced after a KCl pulse. This activation correlates with an increased tyrosine phosphorylation of a 42-kDa protein as determined by antiphosphotyrosine immun...

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    pub_type: 杂志文章


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  • Caffeic acid phenethyl ester reduces neurovascular inflammation and protects rat brain following transient focal cerebral ischemia.

    abstract::Ischemic stroke is a neurovascular disease treatable by thrombolytic therapy, but the therapy has to be initiated within 3 h of the incident. This therapeutic limitation stems from the secondary injury which results mainly from oxidative stress and inflammation. A potent antioxidant/anti-inflammatory agent, caffeic ac...

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    pub_type: 杂志文章


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  • Direct determination of the N-acetyl-L-aspartate synthesis rate in the human brain by (13)C MRS and [1-(13)C]glucose infusion.

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    pub_type: 临床试验,杂志文章


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  • A novel presenilin-2 splice variant in human Alzheimer's disease brain tissue.

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  • Pretreatment of cerebellar granule cells with concanavalin A potentiates quisqualate-stimulated phosphoinositide hydrolysis.

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    journal_title:Journal of neurochemistry

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    authors: Raulli R,Danysz W,Wroblewski JT

    更新日期:1991-06-01 00:00:00

  • Transfer of glutamine between astrocytes and neurons.

    abstract::The export of glutamine from astrocytes, and the uptake of glutamine by neurons, are integral steps in the glutamate-glutamine cycle, a major pathway for the replenishment of neuronal glutamate. We review here the functional and molecular identification of the transporters that mediate this transfer. The emerging pict...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Bröer S,Brookes N

    更新日期:2001-05-01 00:00:00

  • Rosiglitazone up-regulates glial fibrillary acidic protein via HB-EGF secreted from astrocytes and neurons through PPARγ pathway and reduces apoptosis in high-fat diet-fed mice.

    abstract::The anti-diabetic drug and peroxisome proliferator-activated receptor-gamma (PPARγ) agonist, rosiglitazone, alters astrocyte activation; however, its mechanism remains less-known. We hypothesized participation of epidermal growth factor receptor (EGFR), known to control astrocyte reactivity. We first detected that ros...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kushwaha R,Mishra J,Gupta AP,Gupta K,Vishwakarma J,Chattopadhyay N,Gayen JR,Kamthan M,Bandyopadhyay S

    更新日期:2019-06-01 00:00:00

  • Tissue inhibitor of metalloproteinase-2 (TIMP-2) expression is regulated by multiple neural differentiation signals.

    abstract::Neuronal differentiation requires exquisitely timed cell cycle arrest for progenitors to acquire an appropriate neuronal cell fate and is achieved by communication between soluble signals, such as growth factors and extracellular matrix molecules. Here we report that the expression of TIMP-2, a matrix metalloproteinas...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Jaworski DM,Pérez-Martínez L

    更新日期:2006-07-01 00:00:00

  • Major central nervous system myelin glycoprotein of the African lungfish (Protopterus dolloi) cross-reacts with myelin proteolipid protein antibodies, indicating a close phylogenetic relationship with amphibians.

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    journal_title:Journal of neurochemistry

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    authors: Waehneldt TV,Matthieu JM,Jeserich G

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  • Apoptotic cell death of a hybrid motoneuron cell line induced by immunoglobulins from patients with amyotrophic lateral sclerosis.

    abstract::Apoptotic cell death has recently been implicated in diseases involving nonproliferating, terminally differentiated cells such as neurons. Previous experiments have documented that immunoglobulins from patients with amyotrophic lateral sclerosis (ALS) can kill motoneuron-neuroblastoma hybrid cells [ventral spinal cord...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Alexianu ME,Mohamed AH,Smith RG,Colom LV,Appel SH

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  • Three distinct types of monoclonal antibodies after long-term immunization of rats with mouse nerve growth factor.

    abstract::This article reports the results of a systematic investigation of the different types of antibodies produced in the course of a long-term immunization of rats with mouse nerve growth factor (NGF). We have characterized three types of monoclonal antibodies, namely: (1) antibodies that bind to NGF and inhibit its bindin...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Cattaneo A,Rapposelli B,Calissano P

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  • Sialylation enhances the secretion of neurotoxic amyloid-beta peptides.

    abstract::Alzheimer's disease (AD) is characterized by amyloid-beta peptide (Abeta) deposition in the brain. Abeta is produced by sequential cleavage of amyloid precursor protein (APP) by beta-secretase (BACE1: beta-site APP-cleaving enzyme 1) and gamma-secretase. Previously, we demonstrated that BACE1 also cleaves beta-galacto...

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  • Nicotinic modulation of [3H]dopamine release from striatal synaptosomes: pharmacological characterisation.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rapier C,Lunt GG,Wonnacott S

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  • Penelope's web: using alpha-latrotoxin to untangle the mysteries of exocytosis.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Silva JP,Suckling J,Ushkaryov Y

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  • A beta oligomers - a decade of discovery.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Walsh DM,Selkoe DJ

    更新日期:2007-06-01 00:00:00

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Larsson OM,Griffiths R,Allen IC,Schousboe A

    更新日期:1986-08-01 00:00:00

  • The inflammatory cytokine, interleukin-1 beta, mediates loss of astroglial glutamate transport and drives excitotoxic motor neuron injury in the spinal cord during acute viral encephalomyelitis.

    abstract::Astrocytes remove glutamate from the synaptic cleft via specific transporters, and impaired glutamate reuptake may promote excitotoxic neuronal injury. In a model of viral encephalomyelitis caused by neuroadapted Sindbis virus (NSV), mice develop acute paralysis and spinal motor neuron degeneration inhibited by the AM...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Prow NA,Irani DN

    更新日期:2008-05-01 00:00:00

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Flanagan EM,Erickson JB,Viveros OH,Chang SY,Reinhard JF Jr

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Xiong LW,Raymond LD,Hayes SF,Raymond GJ,Caughey B

    更新日期:2001-11-01 00:00:00