Abstract：
:3,4-Dihydroxyphenylethylamine (dopamine) D2 receptor agonists, including BHT 920 and bromocriptine, and the potassium channel opener minoxidil share the property of hyperpolarizing the plasma membrane by activating voltage-dependent potassium channels. These drugs were tested for their ability to inhibit the cyclic AMP formation induced by forskolin either in intact or in broken pituitary cells. In contrast to bromocriptine, which was active in both experimental systems, BHT 920 and minoxidil inhibited the forskolin-induced cyclic AMP formation in intact-cell but not in broken-cell preparations. The effects of BHT 920 were (a) concentration dependent, with a calculated IC50 of 0.7 microM, (b) dopaminergic in nature, being specifically antagonized by sulpiride, (c) not additive with those induced by minoxidil, and (d) less effective in the presence of potassium channel blockers, such as 4-aminopyridine and tetraethylammonium. These data indicate that the inhibition of forskolin-induced cyclic AMP formation by BHT 920 in intact pituitary cells is not a primary consequence of receptor occupation, but a late event, possibly related to the opening of voltage-dependent potassium channels elicited by this drug through the activation of a subtype of dopamine D2 receptors uncoupled to adenylyl cyclase.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Memo M,Pizzi M,Belloni M,Benarese M,Spano Pdoi
10.1111/j.1471-4159.1992.tb11016.xkeywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
1829-35issue
5eissn
0022-3042issn
1471-4159journal_volume
59pub_type
杂志文章abstract：:Using C8 reversed-phase HPLC in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, we have fractionated proteins contained in human CSFs obtained from patients with schizophrenic disorders. When these proteins were electrophoretically blotted onto polyvinylidene difluoride membrane for direct ...
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doi：10.1111/j.1471-4159.1990.tb01898.x
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pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1994.63031003.x
更新日期：1994-09-01 00:00:00
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更新日期：2001-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1989.tb10929.x
更新日期：1989-01-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1982.tb07924.x
更新日期：1982-05-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1992.tb09278.x
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更新日期：2004-08-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-05-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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更新日期：2003-07-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1987-10-01 00:00:00
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更新日期：1995-09-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2003-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2004-11-01 00:00:00
abstract：:Changes in the free fatty acid pool size and fatty acyl chain composition of mitochondrial membrane phospholipids and their relation to disruption of mitochondrial function were examined in rat brains after 30 min of cerebral ischemia (Pulsinelli-Brierley model) and 60 min of normoxic reoxygenation. During ischemia, s...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1046/j.1471-4159.1994.62051921.x
更新日期：1994-05-01 00:00:00
