Abstract:
:Macrophages (M phi) are activated by IFN gamma and are important cellular targets for infection by human and murine cytomegalovirus (MCMV), making it advantageous for CMVs to block IFN gamma-induced M phi differentiation. We found that MCMV infection inhibited IFN gamma regulation of many genes in M phi. MCMV infection blocked IFN gamma responses at the level of transcription without blocking Janus kinase/signal transducer and activator of transcription pathway activation and targeted IFN response factor 1- and class II transactivator-dependent and independent promoters. MCMV did not alter basal transcription from IFN gamma-responsive promoters and left the majority of cellular transcripts unchanged even after 48 h of infection. The effects of MCMV infection were specific to chromosomal rather than transiently transfected promoters. Characterization of the IFN gamma-responsive chromosomal class II transactivator promoter revealed that MCMV infection blocked IFN gamma-induced promoter assembly, allowing the virus to transcriptionally paralyze infected M phi responses while allowing basal transcription to proceed.
journal_name
Proc Natl Acad Sci U S Aauthors
Popkin DL,Watson MA,Karaskov E,Dunn GP,Bremner R,Virgin HW 4thdoi
10.1073/pnas.1835673100keywords:
subject
Has Abstractpub_date
2003-11-25 00:00:00pages
14309-14issue
24eissn
0027-8424issn
1091-6490pii
1835673100journal_volume
100pub_type
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