Abstract:
BACKGROUND:RCAS1 (receptor binding cancer antigen expressed on SiSo cells) is a tumour associated antigen. It is involved in immune evasion by tumour cells, by binding to receptors on cells involved in the immune response, such as T cells and natural killer cells, and inducing apoptosis. High expression of RCAS1 has been demonstrated immunohistochemically in tumours of the cervix, breast, lung, and stomach; however, the expression of RCAS1 has never been investigated in colorectal cancer. AIMS:To investigate the expression of RCAS1 in colorectal cancer and identify at which stages of colorectal carcinogenesis it is expressed. METHODS:Sixty surgically resected colorectal cancer specimens obtained from Rajavithi Hospital, Bangkok, Thailand were studied. RCAS1 expression was detected immunohistochemically using monoclonal anti-RCAS1 antibody. RCAS1 mRNA expression was also investigated by reverse transcription polymerase chain reaction in the freshly isolated tissues, and serum RCAS1 was measured by enzyme linked immunosorbent assay. RESULTS:Staining for the RCAS1 protein was intense in high stages of colorectal cancer, but weak in normal tissues. The RCAS1 mRNA results correlated with the immunohistochemistry results. Positive serum RCAS1 concentrations were found in 10 of 18 patients with stage II disease and 12 of 32 with stage III and IV, but not in patients with stage I disease. All lymph node and liver metastases showed high expression of RCAS1 protein. CONCLUSIONS:RCAS1 appears to be upregulated in high stages of colorectal cancer, both in the serum and the tissue. RCAS1 expression might be a useful additional criterion for staging this cancer.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Leelawat K,Watanabe T,Nakajima M,Tujinda S,Suthipintawong C,Leardkamolkarn Vdoi
10.1136/jcp.56.10.764keywords:
subject
Has Abstractpub_date
2003-10-01 00:00:00pages
764-8issue
10eissn
0021-9746issn
1472-4146journal_volume
56pub_type
杂志文章abstract::Platelet concentrates were prepared for in vitro storage in either Fenwal PL-732 or Cutter CLX platelet packs. The units were stored at 22 degrees C for seven days with either horizontal or tumbler agitation. Measurement of pH, hypotonic shock response and serotonin uptake indicated in vitro viability was well maintai...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.36.12.1382
更新日期:1983-12-01 00:00:00
abstract:AIMS:To investigate the expression of the placental cell-specific associated proteins in infantile haemangioma (IH). METHODS:Immunohistochemical staining was used to investigate the expression of human chorionic gonadotrophin (hCG), human placental lactogen (hPL), human leucocyte antigen-G (HLA-G), cytokeratin 7 (CK7)...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200191
更新日期:2011-10-01 00:00:00
abstract::Urines from patients with symptoms of urinary tract infection yielded mixed growths of different colony types of Escherichia coli. The different colony types were found to be variants of single infecting strains caused by mutation or by phase action. It is suggested that care should be exercised in the interpretation ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.28.9.728
更新日期:1975-09-01 00:00:00
abstract::Tests of iron and folate nutrition have been carried out in early pregnancy and at delivery in 63 Bantu females. Accepted normal values for serum iron, and percentage saturation of transferrin, but not for unsaturated iron-binding capacity, for non-pregnant subjects were found to apply equally well in pregnancy. Conco...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.2.173
更新日期:1966-03-01 00:00:00
abstract:AIM:To compare the sensitivity and staining pattern of the new immunohistochemical antibody to tyrosinase (T311) with S-100, HMB45, and the recently evaluated antibody to melan-A (A103) in a range of melanocytic lesions. METHOD:Archival, formalin fixed, paraffin wax embedded sections from 50 benign and malignant melan...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.54.3.196
更新日期:2001-03-01 00:00:00
abstract::Factor VII levels have been measured in 100 patients with liver disease following parenteral vitamin K1 therapy. There was good agreement between specific factor VII measurements and the one-stage prothrombin time apart from six patients with compensated cirrhosis in whom the prothrombin time was prolonged despite the...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.29.11.971
更新日期:1976-11-01 00:00:00
abstract::Bilateral adrenalectomy produces a reduction in the alkaline phosphatase concentration in rat intestine, which is not prevented by the administration of saline, but is prevented by the administration of cortisone. The administration of A.C.T.H. to normal rats leads to a rise in intestinal alkaline phosphatase levels a...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.20.2.185
更新日期:1967-03-01 00:00:00
abstract:AIMS:The purpose of this study was to compare the DNA-methylation signature in classic chronic Philadelphia negative myeloproliferative neoplasms (MPN), polycythaemia vera (PV) and essential thrombocythaemia (ET), in order to obtain a global insight into DNA-methylation changes associated with these malignancies. METH...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2011-200175
更新日期:2011-11-01 00:00:00
abstract::An antibody was raised against a protein isolated from the cytoplasm of mesothelioma cells. It was subsequently used in an immunoperoxidase procedure on formalin fixed, paraffin embedded tissue sections. A representative sample of benign and malignant tumours from all the systems of the human body was examined. All th...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.9.961
更新日期:1986-09-01 00:00:00
abstract:AIMS:To compare the sensitivity and specificity of new rabbit monoclonal antibody SP3 with those of mouse monoclonal and rabbit polyclonal antibodies using HER2 amplification defined by chromogenic in situ hybridisation (CISH) as the gold standard. METHODS:Serial sections from tissue microarrays (TMAs) containing 84 b...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2007.053892
更新日期:2008-08-01 00:00:00
abstract::The performance of coagulometers in the National Quality Assessment Scheme (NEQAS) surveys of the prothrombin time conducted between 1986 and 1987 was reviewed. There were sufficient data for analysis for eight types of coagulometer used with a single type of thromboplastin reagent and one instrument with an alternati...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.1.97
更新日期:1989-01-01 00:00:00
abstract::The diagnostic value of the Becton Dickinson Radioassay Kit (125I) for the the assay of red cell folate has been investigated. The assay was acceptable with regards to precision but was non-linear with changing packed cell volume. Sensitivity of the assay was satisfactory, with 24 of 25 folate deficient patients givin...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.37.8.888
更新日期:1984-08-01 00:00:00
abstract::A case of squamous cell carcinoma of the terminal ileum with no underlying duplication or inflammatory disorder is described. The neoplasm seemed to have originated from the surface epithelium, invading the wall and metastasising to the regional lymph nodes. The 65 year old patient was free of disease three years afte...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.44.3.253
更新日期:1991-03-01 00:00:00
abstract::Thirteen meningiomas of varying light microscopic features were studied immunohistologically using a panel of monoclonal antibodies directed against epithelial, mesenchymal, and neural components. All 13 meningiomas showed expression of epithelial membrane antigen, vimentin, and S100 protein, as did normal meninges. F...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.39.4.435
更新日期:1986-04-01 00:00:00
abstract::Methods for detecting insulin binding or transporting antibodies are described, involving the use of radioactively labelled insulin (insulin (131)I). These antibodies are detected in a high proportion of patients receiving insulin, particularly those requiring large doses. The protein fractions concerned appear to be ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.16.2.120
更新日期:1963-03-01 00:00:00
abstract:AIMS:Computational pathology platforms incorporate digital microscopy with sophisticated image analysis to permit rapid, continuous measurement of protein expression. We compared two computational pathology platforms on their measurement of breast tumour oestrogen receptor (ER) and progesterone receptor (PR) expression...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2016-204107
更新日期:2017-05-01 00:00:00
abstract::Temporal changes in gastric antibody response were investigated in 113 (51 men, 62 women) patients with confirmed pernicious anaemia. Their ages ranged from 31-92 years (mean (SD 13.2) 66). At diagnosis, parietal cell antibody and intrinsic factor antibody were detected in 90.9% and 39.1% of all patients, respectively...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.42.10.1092
更新日期:1989-10-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the mismatch repair gene expression and their correlation with NF-kB in patients with oral squamous cell carcinoma (SCC). METHODS:Total RNA was isolated from 28 biopsy samples. A control group was composed of 20 volunteers. Differential expression of hMSH2, hMSH6 and NF-kB ge...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206161
更新日期:2020-05-01 00:00:00
abstract::Large cell neuroendocrine carcinoma (LCNEC) is a high grade type of neuroendocrine tumour with an aggressive clinical course. This report describes the first case of LCNEC combined with an adenocarcinoma component in the common bile duct. A 68 year old man presented with jaundice. Severe stenosis of the bile duct was ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.2005.028019
更新日期:2006-01-01 00:00:00
abstract::Components of the blood fibrinolytic system were measured in 43 patients with leukaemia. Increased fibrinolytic activity, indicated by a reduced plasma plasminogen level, was found in 14 patients. The combination of severe thrombocytopenia and increased fibrinolysis appeared to be a determinant in the production of a ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.21.2.136
更新日期:1968-03-01 00:00:00
abstract::Factor VIII-containing materials were administered to four severely affected haemophiliacs twice weekly in doses calculated to raise the factor VIII level to either 15% or 30% of average normal. The pooled results from those patients with statistically similar baseline bleeding frequencies showed a significant reducti...
journal_title:Journal of clinical pathology
pub_type: 临床试验,杂志文章
doi:10.1136/jcp.30.1.65
更新日期:1977-01-01 00:00:00
abstract:BACKGROUND:Breast-conserving surgery currently focuses on improving margin clearance and excision volume, the main pathology report parameters for oncological and cosmetic outcomes. AIM:To quantitatively evaluate discrepancies in surgical and pathological estimates of breast specimen sizes, including the influence of ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章,多中心研究
doi:10.1136/jclinpath-2011-200392
更新日期:2012-03-01 00:00:00
abstract:AIMS:Experience in the use of whole slide imaging (WSI) for primary diagnosis in pathology is very limited. We aimed to determine the accuracy of interpretation of WSI compared with conventional light microscopy (CLM) in the diagnosis of routine gynaecological biopsies. METHODS:All gynaecological specimens (n=452) rec...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202524
更新日期:2015-01-01 00:00:00
abstract::The congenital dyserythropoietic anaemias (CDAs) are a group of rare hereditary disorders characterised by ineffective erythropoiesis and morphological abnormalities in the erythroblasts. Patients may present with jaundice or with symptoms of anaemia, gall stones or iron overload. The diagnosis can be challenging and ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2013-201779
更新日期:2014-04-01 00:00:00
abstract::The only method currently available to perform Neisseria gonorrhoeae antimicrobial susceptibility testing (Ng-AST) requires a viable organism obtained by culture. Reports of in vitro resistance to extended-spectrum cephalosporins, the treatment of choice for gonorrhoea, coupled with increasing gonorrhoea diagnoses is ...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2014-202392
更新日期:2014-11-01 00:00:00
abstract:AIMS:To investigate the association of cagA positivity and non-opsonic neutrophil activation capacity in wild-type Helicobacter pylori strains with peptic ulcer disease or chronic gastritis only. METHODS:Helicobacter pylori were isolated from antral biopsies of 53 consecutive patients with chronic antral gastritis, of...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.53.4.318
更新日期:2000-04-01 00:00:00
abstract::The activities of phosphohexose isomerase and 6-phosphogluconate dehydrogenase have been studied in the vaginal fluid of women from the gynaecological departments. Women from these departments gave a very high false positive rate, though no cases of carcinoma in situ were not detected by estimating phosphohexose isome...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.19.4.378
更新日期:1966-07-01 00:00:00
abstract::Two cases of anticonvulsant megaloblastic anaemia are described, showing features of unusual interest. Though both cases were apparently deficient in folic acid, the Figlu tests were negative. One patient had an extremely low serum B(12) concentration apparently associated with defective B(12) absorption due to defici...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.18.5.593
更新日期:1965-09-01 00:00:00
abstract::A method of typing Pseudomonas pyocyanea is described which makes use of the property of bacteriocine production. The method was applied in an epidemiological study of Ps. pyocyanea infection in a general hospital. It was found that the majority of strains believed on epidemiological grounds to be of common origin wer...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jcp.17.3.236
更新日期:1964-05-01 00:00:00
abstract:INTRODUCTION:The role of single mutations has been extensively studied myelodysplastic syndromes (MDS), but the impact of genetic aberrations in the context of other mutations is less well understood. BCOR is an epigenetic transcriptional corepressor. In MDS, BCOR mutations are rare and certain mutations are associated...
journal_title:Journal of clinical pathology
pub_type: 杂志文章
doi:10.1136/jclinpath-2019-206132
更新日期:2020-04-01 00:00:00