Abstract：
AIMS:Experience in the use of whole slide imaging (WSI) for primary diagnosis in pathology is very limited. We aimed to determine the accuracy of interpretation of WSI compared with conventional light microscopy (CLM) in the diagnosis of routine gynaecological biopsies. METHODS:All gynaecological specimens (n=452) received over a 2-month period at the Department of Pathology of the Hospital Clinic of Barcelona were analysed blindly by two gynaecological pathologists, one using CLM and the other WSI. All slides were digitised in a Ventana iScan HT (Roche diagnostics) at 200×. All discrepant diagnoses were reviewed, and a final consensus diagnosis was established. The results were evaluated by weighted κ statistics for two observers. RESULTS:The level of interobserver agreement between WSI and CLM evaluations was almost perfect (κ value: 0.914; 95% CI 0.879 to 0.949) and increased during the study period: κ value 0.890; 95% CI 0.835 to 0.945 in the first period and 0.941; 95%; CI 0.899 to 0.983 in the second period. Major discrepancies (differences in clinical management or prognosis) were observed in 9 cases (2.0%). All discrepancies consisted of small lesions (8 high grade squamous intraepithelial lesions of the uterine cervix, one lymph node micrometastasis of an ovarian carcinoma) underdiagnosed or missed in the WSI or the CLM evaluation. Discrepancies with no or minor clinical relevance were identified in 3.8% of the biopsies. No discrepancy was related to the poor quality of the WSI image. CONCLUSIONS:Diagnosis of gynaecological specimens by WSI is accurate and may be introduced into routine diagnosis.
journal_name
J Clin Patholjournal_title
Journal of clinical pathologyauthors
Ordi J,Castillo P,Saco A,Del Pino M,Ordi O,Rodríguez-Carunchio L,Ramírez Jdoi
10.1136/jclinpath-2014-202524subject
Has Abstractpub_date
2015-01-01 00:00:00pages
33-9issue
1eissn
0021-9746issn
1472-4146pii
jclinpath-2014-202524journal_volume
68pub_type
杂志文章abstract：:The apparent volumes of distribution of Evans blue and (59)Fe-dextran were determined in 18 studies.(59)Fe-dextran volume was 5% lower than Evans blue volume, a highly significant difference. The rate of loss of (59)Fe dextran from the circulation was significantly less than that of Evans blue. ...
journal_title：Journal of clinical pathology
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doi：10.1136/jcp.23.4.286
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journal_title：Journal of clinical pathology
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doi：10.1136/jcp.37.10.1114
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abstract：:Tuberous sclerosis is a rare disease with classic primary or secondary changes affecting mainly the cerebrum, skin, kidneys, and heart. Such lesions are generally hamartomatous and thus display malignant features only in rare cases. This paper describes four cases of tuberous sclerosis which were unique in their assoc...
journal_title：Journal of clinical pathology
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abstract：:Two types of lymphocyte tubular structures were studied by electron microscopy in 80 patients with classic or definite rheumatoid arthritis (RA). Fifteen patients with unequivocal systemic lupus erythematosus (SLE) and 10 healthy persons were studied as controls. Lymphocyte tubulo-reticular structures were found in 13...
journal_title：Journal of clinical pathology
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doi：10.1136/jcp.30.9.822
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journal_title：Journal of clinical pathology
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更新日期：1984-04-01 00:00:00
abstract：AIMS:To detect cells bearing BRAF mutations in colorectal tumour samples and peritoneal washings, using a mismatch ligation assay (MLA). METHODS:DNA from 46 colorectal tumours was studied. Part of exon 15 of the BRAF gene was amplified using the polymerase chain reaction, and T-->A mutations at codon 600 were detected...
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doi：10.1136/jcp.2004.018655
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journal_title：Journal of clinical pathology
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doi：10.1136/jcp.16.5.445
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journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jclinpath-2014-202612
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journal_title：Journal of clinical pathology
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journal_title：Journal of clinical pathology
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doi：10.1136/jcp.30.12.1147
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journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.47.4.329
更新日期：1994-04-01 00:00:00
abstract：:A pancreatic endocrine tumour arising within a serous cystadenoma is reported. A 49 year old woman was admitted with a history of epigastric pain, nausea, vomiting, and weight loss of two months duration. She had been diabetic for 12 years. An epigastric mass was palpated in the physical examination, and computed tomo...
journal_title：Journal of clinical pathology
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doi：10.1136/jcp.53.10.800
更新日期：2000-10-01 00:00:00
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journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.30.1.40
更新日期：1977-01-01 00:00:00
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journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.42.9.927
更新日期：1989-09-01 00:00:00
abstract：:Investigations into the immunological functions of three patients with chronic mucocutaneous candidiasis were carried out. Phagocytosis of Candida albicans, nitroblue tetrazolium dye reduction, complement, and immunoglobulin levels were normal. Candidacidal assays using the neutrophils of the patients in autologous se...
journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.33.4.370
更新日期：1980-04-01 00:00:00
abstract：:A method is described for assessing thyroid status using Sephadex column chromatography of serum samples with added (125)I triiodothyronine. The method is simple and inexpensive. It gave a correct diagnostic result in 97% of 258 euthyroid, 90% of 80 hyperthyroid, 78% of 59 hypothyroid, and 67% of 50 euthyroid, ill pat...
journal_title：Journal of clinical pathology
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doi：10.1136/jcp.20.2.170
更新日期：1967-03-01 00:00:00
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journal_title：Journal of clinical pathology
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doi：10.1136/jcp.2003.11965
更新日期：2004-02-01 00:00:00
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journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.13.3.220
更新日期：1960-05-01 00:00:00
abstract：AIMS:The development of a technique for simultaneous in situ hybridisation for native mRNA and conventional immunofluorescence for cytoplasmic antigens in routine pathology specimens. METHODS:Cocktails of synthetic deoxyoligonucleotides coding for immunoglobulin J chain and kappa light chain were 3' end labelled enzym...
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doi：10.1136/jcp.45.2.114
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journal_title：Journal of clinical pathology
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journal_title：Journal of clinical pathology
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journal_title：Journal of clinical pathology
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doi：10.1136/jcp.40.4.384
更新日期：1987-04-01 00:00:00
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journal_title：Journal of clinical pathology
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doi：10.1136/jclinpath-2019-205732
更新日期：2019-06-01 00:00:00
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journal_title：Journal of clinical pathology
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journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.47.8.743
更新日期：1994-08-01 00:00:00
abstract：:Five cases of malignant histiocytosis of the intestine and one case of true histiocytic lymphoma were studied using immunohistological techniques. In paraffin sections tumour cells in all cases were shown to contain alpha-1-antitrypsin and to express the leucocyte common antigen. Four of the five cases of malignant hi...
journal_title：Journal of clinical pathology
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doi：10.1136/jcp.39.1.8
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abstract：AIMS:To determine important factors influencing recurrence after local excision of duct carcinoma in situ (DCIS) of the breast. MATERIALS AND METHODS:The extent (size) in millimetres, classification (by cytonuclear grade (NHSBSP system), by extent of necrosis, and by the Van Nuys system), and excision margins of 115 c...
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更新日期：2002-08-01 00:00:00
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journal_title：Journal of clinical pathology
pub_type： 杂志文章
doi：10.1136/jcp.52.11.815
更新日期：1999-11-01 00:00:00
abstract：:The aim of this article is to provide knowledge of the origin of catecholamines and metabolites so that there can be an informed approach to the methods for biochemical screening for a possible phaeochromocytoma; The article includes a review of catecholamine and metadrenaline metabolism, with methods used in biochemi...
journal_title：Journal of clinical pathology
pub_type： 杂志文章,评审
doi：10.1136/jcp.2009.071647
更新日期：2010-08-01 00:00:00
