Abstract:
BACKGROUND:We have shown previously that both erythrocyte production rate (EPR) and plasma erythropoietin (EPO) levels in response to hypoxia or to compounds able to stimulate EPO secretion are very much higher in post-hypoxic (PH) than in hypertransfused (HT) polycythemic mice with similar levels of hematocrit. Since it has been demonstrated that cobalt (Co) treatment rises renal EPO-mRNA and increases plasma EPO levels, the present study was conducted to determine whether there is a difference between PH and HT mice in relation to the erythropoietic response to Co and whether the stimulatory effect of Co on EPO secretion can be blunted by polycythemia. METHODS:Adult female mice of the CF-1 strain were made polycythemic by either exposing them to 270 h of discontinuous hypoxia (18 h/d) in a hypobaric chamber maintained at 456 hPA (PH mice) or by injecting them with 0.8 ml of washed packed red cells on two consecutive days (HT mice). Measurement of the erythrocyte production rate (EPR) was made by RBC-59Fe uptake. Plasma EPO concentration was determined by RIA. Cobalt chloride (CoC12) was dissolved in saline and injected in doses of 4 and 8 umoles/mouse. Recombinant human EPO (HEMAX 4000, Bio Sidus SA, Argentina) was dissolved in PBS + albumin to the desired concentration. RESULTS:By comparison with the corresponding dose-regression line for rHu-EPO, it was estimated that the responses (EPR) (measured as RBC-59Fe incorporation) of PH mice to sc injections of 4 and 8 umoles of CoC12 were equivalent to 95 and 145 mU of rHu-EPO, respectively. The response of HT mice to 4 umoles of the drug was not detectable. At the upper dose level, the response was equivalent to 52 mU of rHuEPO. Plasma immunoreactive EPO (iEPO) titers 12 h after COC12 (8 umoles) were not significantly different between normocythemic and PH mice. The observed values were significantly higher than those found in HT mice. DISCUSSION:These findings demonstrate that EPO production in response to COC12 is depressed by polycythemia when induced by transfusion but not when induced by chronic exposure to hypobaric hypoxia. They also confirm, but not explain the nature of the conditioning effect of exposure to hypoxia which makes the mechanism controlling EPO secretion either more sensitive to EPO-secreting stimuli or unable to recognize the polycythemic state.
journal_name
Haematologicajournal_title
Haematologicaauthors
Alippi RM,Boyer P,Leal T,Barcelò AC,Martinez MP,Bozzini CEkeywords:
subject
Has Abstractpub_date
1992-11-01 00:00:00pages
446-9issue
6eissn
0390-6078issn
1592-8721journal_volume
77pub_type
杂志文章相关文献
HAEMATOLOGICA文献大全abstract:BACKGROUND:Myeloproliferative neoplasms constitute a group of diverse chronic myeloid malignancies that share pathogenic features such as acquired mutations in the JAK2, TET2, CBL and MPL genes. There are recent reports that a JAK2 gene haplotype (GGCC or 46/1) confers susceptibility to JAK2 mutation-positive myeloprol...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.034488
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:There is variability in the outcome of patients with chronic lymphocytic leukemia with apparently the same stage of disease. Identifying genetic variants that influence patients' outcome and response to treatment may provide important insights into the biology of the disease. DESIGN AND METHODS:We investiga...
journal_title:Haematologica
pub_type: 杂志文章,随机对照试验
doi:10.3324/haematol.2011.043471
更新日期:2011-10-01 00:00:00
abstract::Not available. ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2020.266320
更新日期:2021-01-28 00:00:00
abstract::Children with immune thrombocytopenia for ≥6 months completing a romiplostim study received weekly subcutaneous romiplostim (1-10 μg/kg targeting platelet counts of 50-200×109/L) in this extension to examine romiplostim's long-term safety and efficacy. Sixty-five children received romiplostim for a median of 2.6 years...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2018.202283
更新日期:2019-11-01 00:00:00
abstract::Phase I/II studies of gemtuzumab ozogamicin (GO) in pediatric refractory/relapsed acute myeloid leukemia (AML) have been reported. We present the cases of two children with relapsed AML who were treated with GO plus cytarabine, leading to a decrease of minimal residual disease down to levels not previously obtained. T...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2006-03-01 00:00:00
abstract::Differentiation of erythroblasts to mature red blood cells involves dynamic changes of the membrane and cytoskeleton networks that are not fully characterized. Using a mouse fetal liver erythroblast culture system and a targeted shRNA functional screening strategy, we identified a critical role of pleckstrin-2 in acti...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2014.105809
更新日期:2014-07-01 00:00:00
abstract::Some prospective studies showed that rabbit antithymocyte globulin was inferior to horse antithymocyte globulin as first-line therapy for patients with severe aplastic anemia. We retrospectively analyzed the clinical outcome of 455 children with severe aplastic anemia who received horse antithymocyte globulin (n=297) ...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:10.3324/haematol.2013.089268
更新日期:2014-04-01 00:00:00
abstract::B-cell chronic lymphocytic leukemia-related genomic changes were analyzed by karyotyping, fluorescence in situ hybridization, and V(H) gene sequencing in a prospective clinical evaluation. The V(H) mutational status correlated with high-risk cytogenetic aberrations while no such relation could be demonstrated for spec...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Myelodysplastic syndromes (MDS) comprise a group of heterogeneous hematologic disorders with risk of leukemic evolution (LE). The French-American-British (FAB) co-operative group classifies them into five morphologic entities and the International Prognostic Scoring System (IPSS) proposes four...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:
更新日期:2002-01-01 00:00:00
abstract::The success of hematopoietic stem cell transplantation is determined by multiple factors. Additional complexity is conferred by covariables showing time-dependent effects. We evaluated the effect of predictors on competing-risk outcomes after hematopoietic stem cell transplantation in a time-dependent manner. We analy...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2017.183012
更新日期:2018-09-01 00:00:00
abstract::The discovery that the Ten-Eleven Translocation (TET) hydroxylases cause DNA demethylation has fundamentally changed the notion of how DNA methylation is regulated. Clonal analysis of the hematopoetic stem cell compartment suggests that TET2 mutations can be early events in hematologic cancers and recent investigation...
journal_title:Haematologica
pub_type: 杂志文章,随机对照试验
doi:10.3324/haematol.2013.088740
更新日期:2013-12-01 00:00:00
abstract::We reviewed cardiac T2* assessments from 77 thalassemia major patients between the ages of 2.5 and 18 years to study optimal timing of cardiac iron screening by magnetic resonance imaging. No patient under 9.5 years of age showed detectable cardiac iron in contrast to 36% of patients between the ages of 15-18 years ol...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.12513
更新日期:2008-06-01 00:00:00
abstract::β-thalassemia and sickle cell disease are widespread fatal genetic diseases. None of the existing clinical treatments provides a solution for all patients. Two main strategies for treatment are currently being investigated: (i) gene transfer of a normal β-globin gene; (ii) reactivation of the endogenous γ-globin gene....
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2012.082768
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Persistent mixed chimerism represents a state in which recipient and donor cells stably co-exist after hematopoietic stem cell transplantation. However, since in most of the studies reported in literature the engraftment state was observed in the nucleated cells, in this study we determined the donor origin ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2010.031013
更新日期:2011-01-01 00:00:00
abstract::Plasma levels of several soluble factors were assayed in 31 untreated patients with high-grade non-Hodgkin's lymphomas (NHL). The results showed statistically significant higher average levels of interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-2 receptor (IL-2r) and transferrin receptor (...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::The transferability of the T2* technique for measurement of tissue iron between magnetic resonance (MR) scanners is unknown. Heart and liver multi-breath-hold T2* sequences were installed on MR scanners at six different sites. T2* was assessed locally in five or more patients with thalassemia major (n=39), and subject...
journal_title:Haematologica
pub_type: 杂志文章,多中心研究
doi:
更新日期:2006-10-01 00:00:00
abstract::A parent whose child affected by hemophilia develops an inhibitor has to cope with strong emotions and anxieties related to the acceptance of this second diagnosis when just having severe hemophilia seems nothing compared to having antibodies that neutralize factor infusions and the possibility of uncontrolled hemorrh...
journal_title:Haematologica
pub_type: 杂志文章,评审
doi:
更新日期:2000-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Hydroxyurea (HU) is known to increase gamma-globin chain expression in postnatal life. The efficacy of HU treatment in thalassemia patients is still unclear. The aim of this study was to monitor treatment of a large cohort of patients with beta-thalassemia major in order to establish the respo...
journal_title:Haematologica
pub_type: 临床试验,杂志文章,多中心研究
doi:
更新日期:2004-10-01 00:00:00
abstract:BACKGROUND:Chronic lymphocytic leukemia is a neoplastic disorder that arises largely as a result of defective apoptosis leading to chemoresistance. Stromal cell-derived factor-1 and its receptor, CXCR4, have been shown to play an important role in chronic lymphocytic leukemia cell trafficking and survival. DESIGN AND ...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2009.013847
更新日期:2010-07-01 00:00:00
abstract::Daily intravenous arsenic trioxide administered with all-trans retinoid acid, the standard-of-care for acute promyelocytic leukemia, is costly and challenging to administer. ORH-2014 is a novel, oral arsenic trioxide formulation, consisting of micron-size drug particles with rapid dissolution and high bioavailability....
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.229583
更新日期:2020-06-01 00:00:00
abstract::Long non-coding ribonucleic acids (RNAs) are a novel class of RNA molecules, which are increasingly recognized as important molecular players in solid and hematologic malignancies. Herein we investigated whether long non-coding RNA expression is associated with clinical and molecular features, as well as outcome of yo...
journal_title:Haematologica
pub_type: 临床试验,杂志文章
doi:10.3324/haematol.2017.166215
更新日期:2017-08-01 00:00:00
abstract:BACKGROUND:The World Health Organization separates acute erythroid leukemia (erythropoiesis in ≥50% of nucleated bone marrow cells; ≥20% myeloblasts of non-erythroid cells) from other entities with increased erythropoiesis - acute myeloid leukemia with myelodysplasia-related changes (≥20% myeloblasts of all nucleated c...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2011.043687
更新日期:2011-09-01 00:00:00
abstract::Epidemiological evidence suggests that infection is involved in the etiology of common acute lymphoblastic leukemia, either by stimulating an inappropriate immune response or in the form of a classical transforming agent. In an attempt to elucidate the role that infection is playing in this disease, we used representa...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2006-02-01 00:00:00
abstract::A new, sensitive method combining seminested polymerase chain reaction (PCR) and heteroduplex analysis was used to detect follicular lymphoma (FL) cells in peripheral blood. Based on the detection of IgH rearrangement in DNA from peripheral blood leukocytes, the method demonstrated the presence of monoclonal B cells i...
journal_title:Haematologica
pub_type: 信件
doi:
更新日期:2004-10-01 00:00:00
abstract::Patients with chronic lymphocytic leukemia and unmutated immunoglobulin heavy-chain variable region gene (IGHV) have inferior survival from time of treatment in clinical studies. We assessed real-world outcomes based on mutational status and treatment regimen in a nationwide population-based cohort, comprising all 4,1...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.220194
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:There are no widely accepted criteria for the definition of hematopoietic stem cell transplant -associated microangiopathy (TAM). An International Working Group was formed to develop a consensus formulation of criteria for diagnosing clinically significant TAM. DESIGN AND METHODS:The particip...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.10699
更新日期:2007-01-01 00:00:00
abstract::Aplastic anemia is usually treated with immunosuppression or allogeneic transplant, depending on patient and disease characteristics. Syngeneic transplant offers a rare treatment opportunity with minimal transplant-related mortality, and offers an insight into disease mechanisms. We present here a retrospective analys...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2013.091074
更新日期:2013-11-01 00:00:00
abstract::Tumor microenvironment (TME) and limited immune surveillance play important roles in lymphoma pathogenesis. Here, we aimed to characterize immunological profiles of diffuse large B-cell lymphoma (DLBCL), and predict the outcome in response to immunochemotherapy. We profiled the expression of 730 immune-related genes i...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2019.243626
更新日期:2020-02-20 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Loss of heterozygosity (LOH) on the long arm of chromosome 7 (7q) has been frequently reported in several types of human cancer including hematologic malignancies. Moreover, monosomy of chromosome 7 and 7q deletions have been associated in acute myeloid leukemia (AML) with aggressive disease a...
journal_title:Haematologica
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::The regulation of platelets by oxidants is critical for vascular health and may explain thrombotic complications in diseases such as diabetes and dementia, but remains poorly understood. Here, we describe a novel technique combining electron paramagnetic resonance spectroscopy and turbidimetry, which has been utilized...
journal_title:Haematologica
pub_type: 杂志文章
doi:10.3324/haematol.2018.208819
更新日期:2019-09-01 00:00:00