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Colonic epithelial functional phenotype varies with type and phase of experimental colitis.

Abstract：

BACKGROUND & AIMS:Colonic crypt elongation occurs during both chronic colitis and in the recovery phase of acute colitis. The impact of these alterations on epithelial cell functions is not fully defined. METHODS:DNA microarray analysis of freshly isolated colonic epithelial cells (CECs) from acute and chronic colitis was performed, and the results were confirmed by reverse transcription polymerase chain reaction. Localization of the selected molecules was examined by immunohistochemistry using newly generated antibodies. The function of selected molecules detected in this study was examined by administering the specific inhibitors in dextran sodium sulfate (DSS) colitis. RESULTS:Several detoxification-associated molecules, which contribute to prevent inflammation by regulating physiological balance under normal conditions, were markedly down-regulated, and anti-inflammatory molecules, which are not normally expressed, were up-regulated in the CEC under the chronic colitis. Among the detoxification-associated molecules, carbonic anhydrase IV was specifically down-regulated in CEC of Th2- but not Th1-mediated colitis. Functionally, inhibition of carbonic anhydrase activity led to the enhancement of recovery from DSS-induced acute colitis by directly stimulating CEC proliferation. Increased expression of regeneration-associated molecules such as regenerating gene-III gamma was detectable in the CEC of acute and chronic colitis but not in the recovery phase of colitis. The expression of this molecule was restricted in surface epithelium and upper crypts but not lower crypts. CONCLUSIONS:These studies suggest that functional alterations, which result in either the exacerbation or the suppression of colitis, coexist in the CEC during chronic colitis. CEC functions are likely to be differentially regulated in the context of the stage and mechanism of colitis.

journal_name

Gastroenterology

journal_title

Gastroenterology

authors

Mizoguchi E,Xavier RJ,Reinecker HC,Uchino H,Bhan AK,Podolsky DK,Mizoguchi A

doi

10.1016/s0016-5085(03)00665-6

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

148-61

issue

eissn

0016-5085

issn

1528-0012

pii

S0016508503006656

journal_volume

125

pub_type

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Colonic epithelial functional phenotype varies with type and phase of experimental colitis.