当前位置： SCI文献检索 > INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE期刊下所有文献 > Placental growth factor, a member of the VEGF family, contributes to the development of choroidal neovascularization.

Placental growth factor, a member of the VEGF family, contributes to the development of choroidal neovascularization.

Abstract：

PURPOSE:VEGF has been shown to be necessary, but not sufficient alone, for the development of subretinal pathologic angiogenesis. In the current study, the influence of placental growth factor (PlGF), a member of the VEGF family, in human and experimental choroidal neovascularization (CNV) was investigated. METHODS:The presence of VEGF family member mRNA was evaluated by RT-PCR in neovascular membranes extracted during surgery. The spatial and temporal pattern of VEGF isoforms and PlGF mRNA expression were explored by using the laser capture catapulting technique and RT-PCR in a murine laser-induced model and in vitro. PlGF expression was also studied in human donor eyes. The influence of endogenous PlGF was evaluated in deficient mice (PlGF(-/-)) and by antibody-mediated neutralization of the PlGF receptor. RESULTS:Human neovascular membranes consistently expressed VEGF-A, -B, and -C; PlGF; and VEGFR-1 and -2. The VEGF(120) isoform mRNA was primarily induced in early stages of angiogenesis in vivo and in vitro. PlGF mRNA expression was present in the intact choroid and significantly upregulated during the course of experimental CNV. Both deficient PlGF expression in PlGF(-/-) mice and PlGF receptor neutralization in wild-type mice prevented the development of choroidal neovascularization induced by laser. CONCLUSIONS:These observations demonstrate the participation of PlGF in experimental CNV. They identify therefore PlGF as an additional promising target for ocular antiangiogenic strategies.

journal_name

Invest Ophthalmol Vis Sci

journal_title

Investigative ophthalmology & visual science

authors

Rakic JM,Lambert V,Devy L,Luttun A,Carmeliet P,Claes C,Nguyen L,Foidart JM,Noël A,Munaut C

doi

10.1167/iovs.02-1092

keywords:

subject

Has Abstract

pub_date

2003-07-01 00:00:00

pages

3186-93

issue

7

eissn

0146-0404

issn

1552-5783

journal_volume

44

pub_type

杂志文章

相关文献

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE文献大全
  • Blue Cone Monochromacy Caused by the C203R Missense Mutation or Large Deletion Mutations.

    abstract：Purpose:To compare the phenotype of blue cone monochromacy (BCM) caused by large deletion mutations with those having the C203R missense mutation. Methods:BCM patients with large deletion mutations (n = 21; age range, 5-60 years), and with the C203R missense mutation (n = 13; age range, 5-70 years), were studied with ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.18-25280

    authors： Sumaroka A,Garafalo AV,Cideciyan AV,Charng J,Roman AJ,Choi W,Saxena S,Aksianiuk V,Kohl S,Wissinger B,Jacobson SG

    更新日期：2018-12-03 00:00:00

  • Feasibility of Macular Integrity Assessment (MAIA) Microperimetry in Children: Sensitivity, Reliability, and Fixation Stability in Healthy Observers.

    abstract：Purpose:To assess the feasibility of macular integrity assessment (MAIA) microperimetry (MP) in children. Also to establish representative outcome measures (differential light sensitivity, fixation stability, test-retest reliability) for children without visual impairment. Methods:Thirty-three adults and 33 children (...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-20037

    authors： Jones PR,Yasoubi N,Nardini M,Rubin GS

    更新日期：2016-11-01 00:00:00

  • The Effect of Genetic Variants Associated With Age-Related Macular Degeneration Varies With Age.

    abstract：Purpose:The prevalence of age-related macular degeneration (AMD) increases dramatically with age. This large collaborative study investigates the effects of 51 late-AMD-associated genetic variants in different ages, focusing on individuals above the age of 90 years. Methods:The study included 27,996 individuals of the...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.61.14.17

    authors： Schick T,Lorés-Motta L,Altay L,Fritsche LG,den Hollander AI,Fauser S

    更新日期：2020-12-01 00:00:00

  • Neuroendocrinimmune modulation of secretory component production by rat lacrimal, salivary, and intestinal epithelial cells.

    abstract：PURPOSE:To evaluate the kinetics, receptor specificity, molecular basis, and site selectivity of the endocrine and neural regulation of secretory component (SC) synthesis by rat lacrimal gland acinar cells. METHODS:Acinar cells from the rat lacrimal and submandibular glands, as well as epithelial cells (IEC-6) from th...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Lambert RW,Kelleher RS,Wickham LA,Vaerman JP,Sullivan DA

    更新日期：1994-03-01 00:00:00

  • The protective effect of functional connexin43 channels on a human epithelial cell line exposed to oxidative stress.

    abstract：PURPOSE:To determine the role of connexin43 (Cx43) and gap junctional intercellular communication (GJIC) in the response of the human retinal pigment epithelial cell line ARPE-19 to oxidative stress. METHODS:ARPE-19 cells were treated with the chemical oxidant tert-butyl hydroperoxide (t-BOOH), and cell viability was ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.07-0717

    authors： Hutnik CM,Pocrnich CE,Liu H,Laird DW,Shao Q

    更新日期：2008-02-01 00:00:00

  • Asymmetry of focal ERG in human macular region.

    abstract：:Electroretinograms (ERGs) were elicited by hemicircular (half-disc) stimuli to the upper, lower, temporal and nasal maculas of 26 normal subjects, and the amplitudes and implicit times of the ERGs from opposing macular regions were compared. The amplitudes of a-wave, b-wave and oscillatory potentials (OPs) were signif...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Miyake Y,Shiroyama N,Horiguchi M,Ota I

    更新日期：1989-08-01 00:00:00

  • The Nine-Step Minnesota Grading System for Eyebank Eyes With Age Related Macular Degeneration: A Systematic Approach to Study Disease Stages.

    abstract：Purpose:To refine the Minnesota Grading System (MGS) using definitions from the Age-Related Eye Disease Studies (AREDS) into a nine-step grading scale (MGS-9). Methods:A nine-step grading scale descriptive analysis using three key phenotypic features (total drusen area, increased, and decreased pigmentation) of human ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-22161

    authors： Olsen TW,Liao A,Robinson HS,Palejwala NV,Sprehe N

    更新日期：2017-10-01 00:00:00

  • Acute laser lesion effects on acuity sweep VEPs.

    abstract：:Q-switched neodymium-YAG (infrared) laser lesions at energies up to and including retinal hemorrhages were placed under visual control in the parafovea and the fovea of anesthetized monkeys. Visual-evoked potential (VEP) data were obtained by parallel analog (vector voltmeter) techniques from scalp electrodes in respo...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Schmeisser ET

    更新日期：1992-12-01 00:00:00

  • Volume flow across the isolated retinal pigment epithelium of cynomolgus monkey eyes.

    abstract：:The retinal pigment epithelium (RPE)-choroid was isolated from cynomolgus monkey eyes with experimental retinal detachments and the volume flow was determined in vitro using Ussing-type chambers. With zero pressure difference across the membrane, retina-to-choroid volume flow was 5.0 microliter/hr/cm2 in eyes with sub...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Tsuboi S,Pederson JE

    更新日期：1988-11-01 00:00:00

  • Insulin concentrations in aqueous humor after paracentesis and feeding of rabbits.

    abstract：:Although the lens has been shown to have the capacity to respond to insulin in vitro, little is known concerning the biochemical relationships of insulin to the lens in vivo. Therefore we have measured insulin in the aqueous humor of rabbits by a sensitive radioimmunoassay after paracentesis and feeding. The insulin c...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Coulter JB 3rd,Engelke JA,Eaton DK

    更新日期：1980-12-01 00:00:00

  • Polytetrafluoroethylene/polylactide-co-glycolide laminate containing dexamethasone allows delayed adjustable strabismus surgery in a rabbit model.

    abstract：PURPOSE:To determine the usefulness of polytetrafluoroethylene/polylactide-co-glycolide (PTFE/PLGA) laminate containing dexamethasone in delayed adjustable strabismus surgery. METHODS:A prospective, masked-observer, controlled study was performed in rabbits. Fifty-two rabbit eyes were divided into three groups. After ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.05-0796

    authors： Jee JP,Choung HK,Kim CK,Hwang JM

    更新日期：2006-06-01 00:00:00

  • Kinetics of immune cell infiltration in vaccinia virus keratitis.

    abstract：PURPOSE:Vaccinia virus keratitis leading to blindness is a severe complication of smallpox vaccination. The clinical manifestations of vaccinia virus keratitis are similar to those of herpes simplex virus keratitis, a well-studied immunopathologic disease. Vaccinia virus keratitis is likely to involve an immunopatholog...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.09-5107

    authors： Altmann S,Toomey M,Nesbit B,McIntyre K,Covert J,Dubielzig RR,Leatherberry G,Adkins E,Murphy CJ,Brandt CR

    更新日期：2010-09-01 00:00:00

  • Revisiting Ciliary Muscle Tendons and Their Connections With the Trabecular Meshwork by Two Photon Excitation Microscopic Imaging.

    abstract：PURPOSE:To elucidate the anatomy of the trabecular meshwork (TM) and its connection to ciliary muscle (CM) tendons with two photon excitation microscopic (TPEM) imaging. METHODS:The human aqueous outflow pathway was imaged in an unfixed and nonembeded state by using an inverted TPEM. Laser (Ti:Sapphire) was tuned at 8...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-17091

    authors： Park CY,Lee JK,Kahook MY,Schultz JS,Zhang C,Chuck RS

    更新日期：2016-03-01 00:00:00

  • Confirmation of the validity of the psychophysical light scattering factor.

    abstract：PURPOSE:To reevaluate the validity of the light scatter factor (LSF) formula of Paulsson and Sjöstrand, LSF = L/E (M2/M1-1), where L is the target luminance, E is the illuminance of the glare source at the eye, and M2 and M1 are modulation contrast thresholds measured with and without the glare source, respectively. Th...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Whitaker D,Elliott DB,Steen R

    更新日期：1994-01-01 00:00:00

  • Introduction of DNA into the rat and primate trabecular meshwork by fusogenic liposomes.

    abstract：PURPOSE:To evaluate the feasibility of introducing exogenous genes and phosphorothioate oligonucleotides into the anterior chamber tissues of rats and monkeys using the authors' fusogenic liposomes. METHODS:Hemagglutinating virus of Japan liposomes containing LacZ DNA-high-mobility group 1 complexes or fluorescein iso...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Hangai M,Tanihara H,Honda Y,Kaneda Y

    更新日期：1998-03-01 00:00:00

  • Role of Pseudomonas aeruginosa ExsA in penetration through corneal epithelium in a novel in vivo model.

    abstract：PURPOSE:The scarified cornea keratitis model was modified to study Pseudomonas aeruginosa infection of healing corneal epithelium. The new model was then used to study the role of ExsA, a transcriptional activator of P. aeruginosa, in bacterial penetration through injured and healing corneal epithelia. METHODS:Scratch...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.03-0229

    authors： Lee EJ,Evans DJ,Fleiszig SM

    更新日期：2003-12-01 00:00:00

  • Müller cell response to blue light injury of the rat retina.

    abstract：PURPOSE:In addition to photoreceptor degeneration, excessive light causes degenerative alterations in the inner retina and ganglion cell death. A disturbance in osmohomeostasis may be one causative factor for the alterations in the inner retina. Because Müller cells mediate inner retinal osmohomeostasis (mainly through...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.08-1723

    authors： Iandiev I,Wurm A,Hollborn M,Wiedemann P,Grimm C,Remé CE,Reichenbach A,Pannicke T,Bringmann A

    更新日期：2008-08-01 00:00:00

  • Androgen influence on the meibomian gland.

    abstract：PURPOSE:The hypothesis in the study was that androgens control meibomian gland function, regulate the quality and/or quantity of lipids produced by this tissue, and promote the formation of the tear film's lipid layer. To test this hypothesis, a study was conducted to determine whether androgen receptor protein exists ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Sullivan DA,Sullivan BD,Ullman MD,Rocha EM,Krenzer KL,Cermak JM,Toda I,Doane MG,Evans JE,Wickham LA

    更新日期：2000-11-01 00:00:00

  • Ocular absorption of topically applied FK506 from liposomal and oil formulations in the rabbit eye.

    abstract：PURPOSE:To investigate the use of topically applied FK506, a new immunosuppressive compound, systemic and ocular absorption was determined in serum and various ocular tissues. METHODS:Two drops of 20 microliters FK506 were applied using oil dissolved (OD-FK506) or liposome-bound (LIP-FK506) drug. FK506 concentrations ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Pleyer U,Lutz S,Jusko WJ,Nguyen KD,Narawane M,Rückert D,Mondino BJ,Lee VH,Nguyen K

    更新日期：1993-08-01 00:00:00

  • Amplitude increase of the multifocal electroretinogram during light adaptation.

    abstract：PURPOSE:To determine using the multifocal ERG technique whether there are any regional differences in the increase in the amplitude of cone electroretinograms (ERGs) during light adaptation. METHODS:Multifocal ERGs were recorded with the Visual Evoked Response Imaging System from five normal subjects. Thirty-seven hex...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Kondo M,Miyake Y,Piao CH,Tanikawa A,Horiguchi M,Terasaki H

    更新日期：1999-10-01 00:00:00

  • Conjunctiva-associated lymphoid tissue in the human eye.

    abstract：PURPOSE:Mucosa-associated lymphoid tissue (MALT) represents a part of the immune system located at mucosal surfaces. Its presence in the human eye is the point in question in the current study. Its occurrence, components, topography, and probable functional significance in the human conjunctiva and lacrimal drainage sy...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Knop N,Knop E

    更新日期：2000-05-01 00:00:00

  • Blockade of insulin receptor substrate-1 inhibits corneal lymphangiogenesis.

    abstract：PURPOSE:To analyze whether insulin receptor substrate (IRS-1) is involved in lymphatic vessel development and whether IRS-1 blockade can inhibit lymphangiogenesis in vivo. METHODS:The impact of IRS-1 blockade by GS-101 (Aganirsen), an antisense oligonucleotide against IRS-1, on lymphatic endothelial cell (LEC) prolife...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.10-6816

    authors： Hos D,Regenfuss B,Bock F,Onderka J,Cursiefen C

    更新日期：2011-07-29 00:00:00

  • Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Two-Photon Microscopy Techniques.

    abstract：PURPOSE:To visualize and quantify the three-dimensional (3D) spatial relationships of the structures of the aqueous outflow system (AOS) within intact enucleated mouse eyes using spectral two-photon microscopy (TPM) techniques. METHODS:Spectral TPM, including two-photon autofluorescence (TPAF) and second-harmonic gene...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-18491

    authors： Zhang X,Liu N,Mak PU,Pun SH,Vai MI,Masihzadeh O,Kahook MY,Lei TC,Ammar DA

    更新日期：2016-06-01 00:00:00

  • Approach for a Clinically Useful Comprehensive Classification of Vascular and Neural Aspects of Diabetic Retinal Disease.

    abstract：:The Early Treatment Diabetic Retinopathy Study (ETDRS) and other standardized classification schemes have laid a foundation for tremendous advances in the understanding and management of diabetic retinopathy (DR). However, technological advances in optics and image analysis, especially optical coherence tomography (OC...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.17-21873

    authors： Abramoff MD,Fort PE,Han IC,Jayasundera KT,Sohn EH,Gardner TW

    更新日期：2018-01-01 00:00:00

  • Human diabetic neovascular membranes contain high levels of urokinase and metalloproteinase enzymes.

    abstract：PURPOSE:Retinal neovascularization is one of the leading causes of blindness. A crucial event in this process is the remodeling and penetration of the capillary basement membrane by migrating endothelial cells. This process requires proteolysis of basement membrane components by a variety of proteinases. The objective ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Das A,McGuire PG,Eriqat C,Ober RR,DeJuan E Jr,Williams GA,McLamore A,Biswas J,Johnson DW

    更新日期：1999-03-01 00:00:00

  • Exposure to Chlamydia pneumoniae infection and age-related macular degeneration: the Blue Mountains Eye Study.

    abstract：PURPOSE:To assess cross-sectional and longitudinal associations between exposure to Chlamydia pneumoniae infection and age-related macular degeneration (AMD) in the nested case-control sample drawn from the Blue Mountains Eye Study (BMES) cohort. METHODS:The BMES examined 3654 persons aged 49 to 97 years during 1992 t...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.06-1434

    authors： Robman L,Mahdi OS,Wang JJ,Burlutsky G,Mitchell P,Byrne G,Guymer R,Taylor H

    更新日期：2007-09-01 00:00:00

  • Changes in gene expression by trabecular meshwork cells in response to mechanical stretching.

    abstract：PURPOSE:Trabecular meshwork (TM) cells appear to sense changes in intraocular pressure (IOP) as mechanical stretching. In response, they make homeostatic corrections in the aqueous humor outflow resistance, partially by increasing extracellular matrix (ECM) turnover initiated by the matrix metalloproteinases. To unders...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.05-0075

    authors： Vittal V,Rose A,Gregory KE,Kelley MJ,Acott TS

    更新日期：2005-08-01 00:00:00

  • Characteristics of a glycoprotein in the ocular surface glycocalyx.

    abstract：:A monoclonal antibody has been produced that binds to the apical squames (flattened cells) of the rat ocular surface epithelium and to the goblet cells of the conjunctiva. Immunoelectron microscopic localization of the antigen indicates that in apical cells it is present along the apical-microplical membrane in the re...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Gipson IK,Yankauckas M,Spurr-Michaud SJ,Tisdale AS,Rinehart W

    更新日期：1992-01-01 00:00:00

  • Decreased anionic sites in Bruch's membrane of spontaneous and drug-induced diabetes.

    abstract：:The basal laminae of capillaries, glomeruli, and nerves are thickened in diabetes. Previous studies have shown that diabetic tissues produce increased levels of basal lamina collagen and reduced levels of proteoglycans. Since heparan sulfate and chondroitin sulfate proteoglycans are thought to act as anionic barriers ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Caldwell RB,Slapnick SM,McLaughlin BJ

    更新日期：1986-12-01 00:00:00

  • Regional differences in cystine accumulation point to a sutural delivery pathway to the lens core.

    abstract：PURPOSE:To develop an imaging technique that maps the distribution of free cystine in the rat lens at subcellular resolution and enables cystine accumulation to be compared with the expression of the cystine-glutamate exchanger (Xc-). METHODS:Whole lenses were fixed, cryoprotected, and then cryosectioned in either an ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.06-0861

    authors： Li L,Lim J,Jacobs MD,Kistler J,Donaldson PJ

    更新日期：2007-03-01 00:00:00