Abstract:
PURPOSE:Retinal neovascularization is one of the leading causes of blindness. A crucial event in this process is the remodeling and penetration of the capillary basement membrane by migrating endothelial cells. This process requires proteolysis of basement membrane components by a variety of proteinases. The objective of the present study was to determine the expression of proteinases in human retinal tissues showing active neovascularization. METHODS:Epiretinal neovascular membranes surgically removed from patients with proliferative diabetic retinopathy were analyzed by zymography, and the types and amounts of proteinases present in the tissues were determined. Retinas from nondiabetic donor eyes served as control specimens. RESULTS:Both the high- (54 kDa) and low- (33 kDa) molecular-weight forms of urokinase were present at significantly higher levels in neovascular membranes than in normal retinas. The pro forms of the matrix metalloproteinases (MMP) MMP-2 and MMP-9 were significantly elevated in the neovascular membranes in comparison with levels in normal retinas. In addition, the active forms of these enzymes were present in the membranes, whereas there was no detectable level of the active forms in normal retinas. CONCLUSIONS:Human diabetic neovascular membranes contain high levels of urokinase and MMP. The increased activity of proteinases in the final common pathway of retinal neovascularization indicates that inhibition of these enzymes may be a useful therapeutic target as an alternative approach in the management of proliferative retinopathies.
journal_name
Invest Ophthalmol Vis Scijournal_title
Investigative ophthalmology & visual scienceauthors
Das A,McGuire PG,Eriqat C,Ober RR,DeJuan E Jr,Williams GA,McLamore A,Biswas J,Johnson DWkeywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
809-13issue
3eissn
0146-0404issn
1552-5783journal_volume
40pub_type
杂志文章abstract:PURPOSE:To assess the repeatability and reproducibility of central corneal thickness (CCT) measurements obtained by combined scanning-slit/Placido-disc topography (Orbscan II; Bausch & Lomb, Rochester, NY) after laser in situ keratomileusis (LASIK) and to compare the results with another noncontact method, specular mic...
journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章,多中心研究
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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更新日期:1989-03-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:2000-02-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2015-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1986-08-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
pub_type: 杂志文章
doi:
更新日期:1988-12-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1997-07-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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journal_title:Investigative ophthalmology & visual science
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doi:
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更新日期:2013-11-01 00:00:00
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更新日期:2006-10-01 00:00:00
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更新日期:2020-08-03 00:00:00
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journal_title:Investigative ophthalmology & visual science
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更新日期:2016-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-05-01 00:00:00
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journal_title:Investigative ophthalmology & visual science
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doi:
更新日期:1980-11-01 00:00:00
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更新日期:2018-08-01 00:00:00
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更新日期:2014-03-19 00:00:00
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journal_title:Investigative ophthalmology & visual science
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