Abstract:
:We analyzed forensic DNA samples from four cases of sexual assault, using the Y-chromosome-specific human DNA markers and a panel of autosomal short tandem repeats (STRs). The presence of male contribution was evaluated by the analysis of the Amelogenin locus. A panel of tetrameric Y-STR (DYS19, DYS390, DYS391, DYS392, DYS385, DYS389I and II) was used in further analysis of samples, increasing the efficiency of the forensic genetic analyses. It was possible to identify a partial or full Y-profile of the rapists in different DNA mixtures when genetic profile could not be detected by autosomal STRs. However, in the case of male/male DNA mixture, only the victim's Y-profile could be obtained because the DNA of the offenders was present in low amounts. When the mixture contained different male/male proportion of DNA, only the full profile of the major component could be detected. In cases where male/female DNA mixed stains contained a sufficient amount of male DNA, the analysis of autosomal STRs was adequate enough to identify the full profile of the rapist. Our experience shows that the main advantage of the Y-STR approach is its ability to detect the male component in the mixed stains when the DNA of the male contributor is present only in a very small amount.
journal_name
Croat Med Jjournal_title
Croatian medical journalauthors
Cerri N,Ricci U,Sani I,Verzeletti A,De Ferrari Fkeywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
289-92issue
3eissn
0353-9504issn
1332-8166journal_volume
44pub_type
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doi:
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更新日期:2001-10-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2003-04-01 00:00:00
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更新日期:2003-08-01 00:00:00
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