Comparison of standard PCR and the LightCycler technique to determine the thrombophilic mutations: an efficiency and cost study.

abstract：BACKGROUND:The measurement of serum IgE aids in the diagnosis and management of atopic allergic disease and hyper-IgE immunodeficiency syndromes. The 2nd World Health Organization (WHO) International Reference Reagent (IRR) for serum IgE (75/502; 5000 IU/ampoule), is widely used to calibrate assays for serum IgE. Exhau...

journal_title：Clinical chemistry and laboratory medicine

authors： Thorpe SJ,Heath A,Fox B,Patel D,Egner W

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:High-density lipoprotein (HDL) is believed to be protective against coronary heart disease (CHD). HDL is comprised of different subfractions. Of these, HDL 2b is believed to be the most important in preventing CHD. Current methods for HDL subfraction measurements are not standardized and often exhibit poor a...

journal_title：Clinical chemistry and laboratory medicine

authors： Yang DH,Konigshofer Y,Dike J,Chang E,Mueller O

更新日期：2010-06-01 00:00:00

abstract：:A questionable scientific approach to measuring at low concentrations and inappropriate censoring of results below certain cut-offs have resulted in the dichotomous classification of troponin assays based on their so-called analytical sensitivity. The definition of "high-sensitivity" cardiac troponin is flawed. Eviden...

journal_title：Clinical chemistry and laboratory medicine

authors： Ungerer JPJ,Pretorius CJ

更新日期：2017-10-26 00:00:00

abstract：BACKGROUND:As ABO blood type influences the plasma level of coagulation factor VIII (FVIII), it likely also affects activated partial thromboplastin time (aPTT) and thrombin generation assay (TGA) values. Here, we aimed to investigate the effect of ABO type on the normal values of three global coagulation assays: proth...

journal_title：Clinical chemistry and laboratory medicine

authors： Choi Q,Kim JE,Kim SY,Han KS,Kim HK

更新日期：2015-08-01 00:00:00

abstract：Background:Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assays are employed in more and more clinical laboratories to quantify steroids. The steroid quantification by LC-MS/MS shows great value in screening or diagnosing endocrine disorders; however, the number of functional steroids included in the ...

journal_title：Clinical chemistry and laboratory medicine

authors： Wang Z,Wang H,Peng Y,Chen F,Zhao L,Li X,Qin J,Li Q,Wang B,Pan B,Guo W

更新日期：2020-02-21 00:00:00

abstract：BACKGROUND:Urinary enzymes, including the brush border enzyme alanine aminopeptidase (AAP), are reliable biomarkers of nephrotoxicity and represent early indicators of tubular damage. The aim of the present study was to develop and validate an automated urinary AAP activity assay. METHODS AND RESULTS:For this purpose,...

journal_title：Clinical chemistry and laboratory medicine

authors： Holdt B,Peters E,Nagel HR,Steiner M

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Improving quality and patient safety in the medical biochemistry laboratory accredited according to the International Standard Organization (ISO 15189:2012) requires the patient-centered evaluation of errors based on the implementation of quality indicators (QIs) across the total testing process. Our main go...

journal_title：Clinical chemistry and laboratory medicine

authors： Flegar-Meštrić Z,Perkov S,Radeljak A,Kardum Paro MM,Prkačin I,Devčić-Jeras A

更新日期：2017-03-01 00:00:00

abstract：:Transferrin concentration and total iron binding capacity (TIBC) are currently used to assess iron status. Although correlation between TIBC and transferrin is generally considered as good, conversion factors between the two analytes found in literature show large differences. Although the price per test is lower for ...

journal_title：Clinical chemistry and laboratory medicine

authors： Kasvosve I,Delanghe J

更新日期：2002-10-01 00:00:00

abstract：:Choroid plexus has the highest concentration of transthyretin (TTR) mRNA in the body, 4.4 microg TTR mRNA/g wet weight tissue, compared with 0.39 microg in the liver. The proportion of TTR to total protein synthesis in choroid plexus is 12%. All newly synthesized TTR is secreted towards the ventricles. Net transfer of...

journal_title：Clinical chemistry and laboratory medicine

authors： Schreiber G

更新日期：2002-12-01 00:00:00

abstract：BACKGROUND:Exosomes are cytoplasm containing vesicles released by many cells that can be found in several biological fluids including urine. Urinary exosomes are released from every segment of the nephron, are detectable in urine, constitutively contain RNA (small RNAs and mRNAs) and harbor unique subset of proteins, r...

journal_title：Clinical chemistry and laboratory medicine

authors： Channavajjhala SK,Rossato M,Morandini F,Castagna A,Pizzolo F,Bazzoni F,Olivieri O

更新日期：2014-03-01 00:00:00

abstract：:There is currently no well-established biomarker for Parkinson's disease. The need to better diagnose the condition, define the subtypes of disease, and follow its course independent of any symptomatic drug effects is well-established. In this review, we will begin by reviewing the evidence for biological fluid biomar...

journal_title：Clinical chemistry and laboratory medicine

authors： Breen DP,Michell AW,Barker RA

更新日期：2011-03-01 00:00:00

abstract：:The preterm premature rupture of membranes (PPROM) is a common condition in pregnant women and is associated with significant maternal and perinatal morbidity. Most of the time, the diagnosis is done during physical examination. However, in 10%-20% of equivocal cases, biological markers are needed to confirm the diagn...

journal_title：Clinical chemistry and laboratory medicine

authors： Marcellin L,Goffinet F

更新日期：2012-04-14 00:00:00

abstract：BACKGROUND:Detection of systemic sclerosis-associated autoantibodies (SSc-Ab) is mostly restricted to anti-centromere and anti-topoisomerase-I. However, anti-RNA-polymerase-III and anti-PM/Scl are also important diagnostic markers for the disease supporting their incorporation in the laboratory repertoire. The aim of t...

journal_title：Clinical chemistry and laboratory medicine

authors： Bonroy C,Smith V,Van Steendam K,Van Praet J,Deforce D,Devreese K,De Keyser F

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND:Sickle cell disease is a major health burden in India. The aim of the study was to compare the diagnostic utility of two different approaches on automated high performance liquid chromatography (HPLC) for newborn screening for sickle cell disorders and other haemoglobinopathies in India. METHODS:Newborn bab...

journal_title：Clinical chemistry and laboratory medicine

authors： Upadhye DS,Jain DL,Trivedi YL,Nadkarni AH,Ghosh K,Colah RB

更新日期：2014-12-01 00:00:00

abstract：BACKGROUND:Elevated plasma total homocysteine is a risk factor for cardiovascular disease (CVD), but the randomized trials of dietary supplementation with B-vitamins to lower homocysteine have not yet provided clear evidence of benefit on vascular risk. METHODS:Cumulative meta-analysis of all randomized trials assessi...

journal_title：Clinical chemistry and laboratory medicine

authors： Clarke R,Armitage J,Lewington S,Collins R,B-Vitamin Treatment Trialists' Collaboration.

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Thrombopoietin (TPO), a growth factor primarily involved in regulating thrombopoiesis, has been recently implicated in the pathogenesis of sepsis. TPO levels are, indeed, greatly increased in patients with sepsis compared to control subjects, and correlate with sepsis severity. The aim of this study was to e...

journal_title：Clinical chemistry and laboratory medicine

authors： Segre E,Pigozzi L,Lison D,Pivetta E,Bosco O,Vizio B,Suppo U,Turvani F,Morello F,Battista S,Moiraghi C,Montrucchio G,Lupia E

更新日期：2014-10-01 00:00:00

abstract：:Acute intestinal ischemic injury (i3) is a life-threatening condition with disastrous prognosis, which is currently difficult to diagnose at the early stages of the disease; a rapid diagnosis is mandatory to avoid irreversible ischemia, extensive bowel resection, sepsis and death. The overlapping protein expression of...

journal_title：Clinical chemistry and laboratory medicine

authors： Peoc'h K,Nuzzo A,Guedj K,Paugam C,Corcos O

更新日期：2018-02-23 00:00:00

abstract：:Phenylketonuria is an inherited metabolic disease, which is characterized by an increased level of serum phenylalanine. Quantitative measurement of phenylalanine in the serum of phenylketonuria patients is necessary to confirm the disease, and to distinguish phenylketonuria from other forms of hyperphenylalaninemia. I...

journal_title：Clinical chemistry and laboratory medicine

authors： Vallian S,Moeini H

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Recent studies have suggested that omentin-1 plays a critical role in the development of cardiovascular disease. However, reported findings are inconsistent, and no study has evaluated the association between omentin-1 levels and a poor functional outcome after ischemic stroke onset. METHODS:A total of 266 ...

journal_title：Clinical chemistry and laboratory medicine

authors： Xu T,Zuo P,Wang Y,Gao Z,Ke K

更新日期：2018-01-26 00:00:00

abstract：:An alcohol-associated change in the serum transferrin glycoform pattern, carbohydrate-deficient transferrin (CDT), is used as a biomarker of chronic moderate to heavy alcohol consumption. A current limitation in CDT analysis is the lack of standardization, which hampers clinical and analytical comparison between studi...

journal_title：Clinical chemistry and laboratory medicine

authors： Jeppsson JO,Arndt T,Schellenberg F,Wielders JP,Anton RF,Whitfield JB,Helander A,International Federation of Clinical Chemistry and Laboratory Medicine Working Group on Standardization of Carbohydrate-deficient Transferin (IFCC-WG-CDT).

更新日期：2007-01-01 00:00:00

abstract：:Molecular diagnostics is one of the most rapidly growing areas of laboratory medicine. This rapid growth of clinical molecular tests has outpaced the availability and development of reference methods and reference materials. Such methods and materials are important for the development, validation, and interpretation o...

journal_title：Clinical chemistry and laboratory medicine

authors： Payne DA,Mamotte CD,Gancberg D,Pazzagli M,van Schaik RH,Schimmel H,Rousseau F,IFCC Committee for Molecular Diagnostics (C-MD).

更新日期：2010-11-01 00:00:00

abstract：Objectives:The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread globally. The laboratory diagnosis of SARS-CoV-2 infection has relied on nucleic acid testing; however, it has some limitations, such as low throughput and high rates of false negatives. Tests of higher sensitivit...

journal_title：Clinical chemistry and laboratory medicine

authors： Qian C,Zhou M,Cheng F,Lin X,Gong Y,Xie X,Li P,Li Z,Zhang P,Liu Z,Hu F,Wang Y,Li Q,Zhu Y,Duan G,Xing Y,Song H,Xu W,Liu BF,Xia F

更新日期：2020-07-15 00:00:00

abstract：BACKGROUND:High-dose biotin therapy is beneficial in progressive multiple sclerosis (MS) and is expected to be adopted by a large number of patients. Biotin therapy leads to analytical interference in many immunoassays that utilize streptavidin-biotin capture techniques, yielding skewed results that can mimic various e...

journal_title：Clinical chemistry and laboratory medicine

authors： Piketty ML,Prie D,Sedel F,Bernard D,Hercend C,Chanson P,Souberbielle JC

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND:The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods. METHOD:Spectrophotometry was used. RESULTS:Jaffe pro...

journal_title：Clinical chemistry and laboratory medicine

authors： Chromý V,Rozkosná K,Sedlák P

更新日期：2008-01-01 00:00:00

abstract：:There are large differences in the use of laboratory tests between hospitals in Sweden. These differences are not only due to differences between the patients treated but also to differences in practice. Use of laboratory test seems to reflect local traditions to a large extent. These large variations in practice are ...

journal_title：Clinical chemistry and laboratory medicine

authors： Larsson A,Palmer M,Hultén G,Tryding N

更新日期：2000-05-01 00:00:00

abstract：BACKGROUND:The aim was to investigate whether first trimester glycodelin and angiopoietin-2 can predict small-for-gestational age (SGA) at delivery, individually or in combination. METHODS:In this case-control study we measured glycodelin and angiopoietin-2 on serum from 170 singleton pregnant women delivering SGA neo...

journal_title：Clinical chemistry and laboratory medicine

authors： Hansen YB,Myrhøj V,Jørgensen FS,Sørensen S

更新日期：2016-09-01 00:00:00

abstract：:This paper reports on a pilot external quality assessment scheme for bone markers including serum bone alkaline phosphatase and procollagen 1-carboxy terminal propeptide together with urine deoxypyridinoline, N- and C-telopeptides. The data shows poor numerical agreement between commercial assays, between-laboratory i...

journal_title：Clinical chemistry and laboratory medicine

authors： Whitham KM,Milford-Ward A

更新日期：2000-11-01 00:00:00

abstract：BACKGROUND:Asymmetric dimethylarginine (ADMA), an endogenous competitive inhibitor of nitric oxide synthase plays an important role in endothelial dysfunction processes. Recent studies have linked high ADMA levels with several pathological conditions. The interest as a marker of endothelial dysfunction has increased in...

journal_title：Clinical chemistry and laboratory medicine

authors： Gervasoni J,Bonelli F,Zuppi C,Zappacosta B,Mordente A,Calvani R,Persichilli S

更新日期：2011-09-06 00:00:00

abstract：BACKGROUND:Several drugs may affect the diagnosis of brain death by depressing the electroencephalographic signal. Serum levels of these drugs must be below their respective therapeutic ranges. METHODS:A high performance liquid chromatography-based fast and simple method was developed for determination of thiopentone,...

journal_title：Clinical chemistry and laboratory medicine

authors： Meinitzer A,Kalcher K,Gartner G,Halwachs-Baumann G,März W,Stettin M

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Aging is the result of several mechanisms which operate simultaneously. Among them, glycation is of particular interest because it is a reaction which affects slowly renewing tissues and macromolecules with elevated half-life, like the dermis, a skin compartment highly affected by aging. Glycation produces c...

journal_title：Clinical chemistry and laboratory medicine

authors： Pageon H,Zucchi H,Rousset F,Monnier VM,Asselineau D

更新日期：2014-01-01 00:00:00