Abstract:
:The Kruppel-like factor 5 (KLF5/IKLF) belongs to the Kruppel family of genes which bind GC-rich DNA elements and activate or repress their target genes in a promoter context and/or cellular environment-dependent manner. In the present study, we used the Gal4 fusion assay system to characterize the mechanism of transactivation by KLF5. We demonstrated that the transactivation function of KLF5 was enhanced by CREB-binding protein (CBP) and blocked by wild-type but not mutant E1A. Over expression of CBP reversed the inhibition effect of E1A. With various lengths of KLF5 fusion protein, the transactivation functions were localized to 156 amino acid residues at the N-terminal region and 133 amino acid residues adjacent to the Zn finger motif. We mapped the CBP and KLF5 interaction domain to the N-terminal region of CBP (amino acids 1-232) and the N-terminal region of KLF5 (amino acids 1-238) where one of the activation functions resides. The histone acetyltransferase (HAT) activity of CBP does not play a role in the transactivation function of KLF5 nor does it acetylate KLF5 in vitro. However, phosphorylation is important in KLF5 transactivation activity. Inhibition of protein kinase activity by H7 or calphostin C blocked both full-length and N-terminal fragment (amino acids 1-238) KLF5 activities. Mutation at a potential protein kinase C phosphorylation site within the CBP interaction domain of KLF5 reduces its transactivation function. Furthermore, using the GST pull-down approach, we showed that phosphorylation of KLF5 enhances its interaction with CBP. The results of the present study provide a mechanism for KLF5 transactivation function.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Zhang Z,Teng CTdoi
10.1093/nar/gkg310keywords:
subject
Has Abstractpub_date
2003-04-15 00:00:00pages
2196-208issue
8eissn
0305-1048issn
1362-4962journal_volume
31pub_type
杂志文章abstract::The molecular mechanism in pancreatic β cells underlying hyperlipidemia and insulin insufficiency remains unclear. Here, we find that the fatty acid-induced decrease in insulin levels occurs due to a decrease in insulin translation. Since regulation at the translational level is generally mediated through RNA-binding ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky867
更新日期:2018-12-14 00:00:00
abstract::mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Us...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.19.4538
更新日期:1998-10-01 00:00:00
abstract::Post-transcriptional methylation of ribose at position O-2' is one of the most common and conserved types of RNA modification. Details of the functional roles of these methylations are far from clear, although in tRNA they are involved at position 34 in regulation of codon recognition and in eukaryotic rRNAs they are ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.18.e20
更新日期:1999-09-15 00:00:00
abstract::The Mouse Genome Database (MGD) (http://www.informatics.jax.org) is the community model organism database resource for the laboratory mouse, a premier animal model for the study of genetic and genomic systems relevant to human biology and disease. MGD maintains a comprehensive catalog of genes, functional RNAs and oth...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1225
更新日期:2014-01-01 00:00:00
abstract::We have determined the stability of intramolecular DNA quadruplexes in which the four G(3)-tracts are connected by non-nucleosidic linkers containing propanediol, octanediol or hexaethylene glycol, replacing the TTA loops in the human telomeric repeat sequence. We find that these sequences all fold to form intramolecu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh598
更新日期:2004-05-11 00:00:00
abstract::Codon usage bias is a universal feature of all genomes. Although codon usage has been shown to regulate mRNA and protein levels by influencing mRNA decay and transcription in eukaryotes, little or no genome-wide correlations between codon usage and mRNA levels are detected in mammalian cells, raising doubt on the sign...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1253
更新日期:2021-01-25 00:00:00
abstract::Polyadenylation of nascent transcripts is one of the key mRNA processing events in eukaryotic cells. A large number of human and mouse genes have alternative polyadenylation sites, or poly(A) sites, leading to mRNA variants with different protein products and/or 3'-untranslated regions (3'-UTRs). PolyA_DB 2 contains p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl870
更新日期:2007-01-01 00:00:00
abstract::Here we examined the role of cellular vitamin C in genotoxicity of carcinogenic chromium(VI) that requires reduction to induce DNA damage. In the presence of ascorbate (Asc), low 0.2-2 microM doses of Cr(VI) caused 10-15 times more chromosomal breakage in primary human bronchial epithelial cells or lung fibroblasts. D...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl1069
更新日期:2007-01-01 00:00:00
abstract::Much effort has long been devoted to unraveling the coordinated cellular response to genotoxic insults. In view of the difficulty of obtaining human biological samples of homogeneous origin, I have established a set of stable human clones where one DNA repair gene has been stably silenced by means of RNA interference....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm195
更新日期:2007-01-01 00:00:00
abstract::Human tissue-specific genes were reported to be longer than housekeeping genes (both in coding and intronic parts). The competing neutralist and adaptationist models were proposed to explain this observation. Here I show that in human genome the longest are genes with the intermediate expression pattern. From the stan...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl773
更新日期:2006-01-01 00:00:00
abstract::Cytosines in genomic DNA are sometimes methylated. This affects many biological processes and diseases. The standard way of measuring methylation is to use bisulfite, which converts unmethylated cytosines to thymines, then sequence the DNA and compare it to a reference genome sequence. We describe a method for the cri...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks275
更新日期:2012-07-01 00:00:00
abstract::Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light microscopic particle observation is usually limited to two-dimensional projections of short trajectory segments, we report here imag...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1194
更新日期:2015-01-01 00:00:00
abstract::ATF4 is a pro-oncogenic transcription factor whose translation is activated by eIF2 phosphorylation through delayed re-initiation involving two uORFs in the mRNA leader. However, in yeast, the effect of eIF2 phosphorylation can be mimicked by eIF5 overexpression, which turns eIF5 into translational inhibitor, thereby ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw559
更新日期:2016-10-14 00:00:00
abstract::The active and inactive state of transcription factors in growing cells is usually directed by allosteric physicochemical signals or metabolites, which are in turn either produced in the cell or obtained from the environment by the activity of the products of effector genes. To understand the regulatory dynamics and t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm743
更新日期:2007-01-01 00:00:00
abstract::Guanine-quadruplexes (G4) consist of non-canonical four-stranded helical arrangements of guanine-rich nucleic acid sequences. The bulky and thermodynamically stable features of G4 structures have been shown in many respects to affect normal nucleic acid metabolism. In vivo conversion of G4 structures to single-strande...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr630
更新日期:2011-11-01 00:00:00
abstract::In a bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP)/transcription factor binding screen, we identified Mycobacterium smegmatis Ms6479 as the first c-di-GMP-responsive transcriptional factor in mycobacteria. Ms6479 could specifically bind with c-di-GMP and recognize the promoters of 37 lipid transport an...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks923
更新日期:2012-12-01 00:00:00
abstract::A 14 kb maize DNA fragment carrying nuclear rRNA genes and spacer regions was isolated and characterised by restriction enzyme mapping. A complete 3020 bp long external spacer region was sequenced and revealed 9 tandemly arranged 200 bp long repeat units with high homology. The repeat units lie upstream from two promi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.12.4969
更新日期:1986-06-25 00:00:00
abstract::Chinese hamster genomic DNA sequences from the region downstream of the dihydrofolate reductase (DHFR) gene reported to contain a chromosomal origin of bidirectional DNA replication (OBR-1) were tested for their ability to support autonomous DNA replication in human cells. A 13.3 kilobase fragment containing OBR-1 and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.22.5971
更新日期:1992-11-25 00:00:00
abstract::The DSSP program automatically assigns the secondary structure for each residue from the three-dimensional co-ordinates of a protein structure to one of eight states. However, discrete assignments are incomplete in that they cannot capture the continuum of thermal fluctuations. Therefore, DSSPcont (http://cubic.bioc.c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg626
更新日期:2003-07-01 00:00:00
abstract::A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.6.1499
更新日期:1981-03-25 00:00:00
abstract::Metnase is a human protein with methylase (SET) and nuclease domains that is widely expressed, especially in proliferating tissues. Metnase promotes non-homologous end-joining (NHEJ), and knockdown causes mild hypersensitivity to ionizing radiation. Metnase also promotes plasmid and viral DNA integration, and topoisom...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq339
更新日期:2010-09-01 00:00:00
abstract::Invariance of temporal order of genome replication in eukaryotic cells and its correlation with gene activity has been well-documented. However, recent data suggest a relax control of replication timing. To evaluate replication schedule accuracy, we detailed the replicational organization of the developmentally regula...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm586
更新日期:2007-01-01 00:00:00
abstract::A novel expression system was established that allows expression and propagation of the human hepatitis B virus (HBV) genome in cultured animal cells. An overlength HBV genome encoding the viral pregenomic RNA was put under transcriptional control of the human metallothionein IIA promoter thereby replacing the endogen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.24.10117
更新日期:1987-12-23 00:00:00
abstract::Histone post-translational modifications have been shown to contribute to DNA damage repair. Prior studies have suggested that specific H3K79 methylation states play distinct roles in the response to UV-induced DNA damage. To evaluate these observations, we examined the effect of altered H3K79 methylation patterns on ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku242
更新日期:2014-06-01 00:00:00
abstract::PIECE (Plant Intron Exon Comparison and Evolution) is a web-accessible database that houses intron and exon information of plant genes. PIECE serves as a resource for biologists interested in comparing intron-exon organization and provides valuable insights into the evolution of gene structure in plant genomes. Recent...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw935
更新日期:2017-01-04 00:00:00
abstract::A crucial component of the human mitochondrial DNA replisome is the ring-shaped helicase TWINKLE-a phage T7-gene 4-like protein expressed in the nucleus and localized in the human mitochondria. Our previous studies showed that despite being a helicase, TWINKLE has unique DNA annealing activity. At the time, the implic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw098
更新日期:2016-05-19 00:00:00
abstract::Riboswitches are important gene regulatory elements frequently encountered in bacterial mRNAs. The recently discovered nadA riboswitch contains two similar, tandemly arrayed aptamer domains, with the first domain possessing high affinity for nicotinamide adenine dinucleotide (NAD+). The second domain which comprises t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1029
更新日期:2020-12-02 00:00:00
abstract::While most of the recent improvements in multiple sequence alignment accuracy are due to better use of vertical information, which include the incorporation of consistency-based pairwise alignments and the use of profile alignments, we observe that it is possible to further improve accuracy by taking into account alig...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn945
更新日期:2009-02-01 00:00:00
abstract::The genome stability is maintained by coordinated action of DNA repairs and checkpoints, which delay progression through the cell cycle in response to DNA damage. Rad9 is conserved from yeast to human and functions in cell cycle checkpoint controls. Here, a regulatory mechanism for Rad9 function is reported. In this s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq1264
更新日期:2011-06-01 00:00:00
abstract::In HIV-1 infection, Tat acts at least in part to control transcriptional elongation by overcoming premature transcriptional termination. In some other genes this process is governed by DNA elements called attenuators in concert with cellular transcription factors. To understand the action of Tat more fully and explore...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.24.5017
更新日期:1997-12-15 00:00:00