Superoxo, mu-peroxo, and mu-oxo complexes from heme/O2 and heme-Cu/O2 reactivity: copper ligand influences in cytochrome c oxidase models.

abstract：:There are some ∼1,100 known antimicrobial peptides (AMPs), which permeabilize microbial membranes but have diverse sequences. Here, we develop a support vector machine (SVM)-based classifier to investigate ⍺-helical AMPs and the interrelated nature of their functional commonality and sequence homology. SVM is used to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee EY,Fulan BM,Wong GC,Ferguson AL

更新日期：2016-11-29 00:00:00

abstract：:In aqueous solution, aldehydes, and to a lesser extent ketones, hydrate to form geminal diols. We investigate the hydration of methylglyoxal (MG) in the gas phase, a process not previously considered to occur in water-restricted environments. In this study, we spectroscopically identified methylglyoxal diol (MGD) and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Axson JL,Takahashi K,De Haan DO,Vaida V

更新日期：2010-04-13 00:00:00

abstract：:Male infertility, affecting as many as 10% of the adult population, is an extremely prevalent disorder. In most cases, the cause of the condition is unknown, and genetic factors that might affect male fertility, other than some sequences on the Y chromosome, have not been identified. We report here that male mice hete...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang LS,Voyiaziakis E,Chen HL,Rubin EM,Gordon JW

更新日期：1996-10-01 00:00:00

abstract：:Here we test the hypothesis that high-altitude native resident Tibetan women with genotypes for high oxygen saturation of hemoglobin, and thus less physiological hypoxic stress, have higher Darwinian fitness than women with low oxygen saturation genotypes. Oxygen saturation and genealogical data were collected from re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beall CM,Song K,Elston RC,Goldstein MC

更新日期：2004-09-28 00:00:00

abstract：:We have studied the properties of r-eag voltage-activated potassium channels in a stably transfected human embryonic kidney cell line. It was found that r-eag channels are rapidly and reversibly inhibited by a rise in intracellular calcium from 30 to 300 nM. The inhibition does not appear to depend on the activity of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stansfeld CE,Röper J,Ludwig J,Weseloh RM,Marsh SJ,Brown DA,Pongs O

更新日期：1996-09-03 00:00:00

abstract：:Focally evoked calcium waves in astrocyte cultures have been thought to propagate by gap-junction-mediated intercellular passage of chemical signal(s). In contrast to this mechanism we observed isolated astrocytes, which had no physical contact with other astrocytes in the culture, participating in a calcium wave. Thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hassinger TD,Guthrie PB,Atkinson PB,Bennett MV,Kater SB

更新日期：1996-11-12 00:00:00

abstract：:Marine Archaea are important players among microbial plankton and significantly contribute to biogeochemical cycles, but details regarding their community structure and long-term seasonal activity and dynamics remain largely unexplored. In this study, we monitored the interannual archaeal community composition of abun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hugoni M,Taib N,Debroas D,Domaizon I,Jouan Dufournel I,Bronner G,Salter I,Agogué H,Mary I,Galand PE

更新日期：2013-04-09 00:00:00

abstract：:Serine is the only amino acid that is encoded by two disjoint codon sets so that a tandem substitution of two nucleotides is required to switch between the two sets. Previously published evidence suggests that, for the most evolutionarily conserved serines, the codon set switch occurs by simultaneous substitution of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rogozin IB,Belinky F,Pavlenko V,Shabalina SA,Kristensen DM,Koonin EV

更新日期：2016-11-15 00:00:00

abstract：:Homozygous lymphoblastoid cell lines representing Dw subtypes of the DR2 serotype were studied for structural polymorphism at DR beta. These subtypes included Dw2, Dw12, and non-Dw2/non-Dw12. Analysis by two-dimensional gel electrophoresis showed that two DR beta genes were expressed in each cell line studied. One of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee BS,Rust NA,McMichael AJ,McDevitt HO

更新日期：1987-07-01 00:00:00

abstract：:The growth of actin filament networks is a fundamental biological process that drives a variety of cellular and intracellular motions. During motility, eukaryotic cells and intracellular pathogens are propelled by actin networks organized by nucleation-promoting factors that trigger the formation of nascent filaments ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaevitz JW,Fletcher DA

更新日期：2007-10-02 00:00:00

abstract：:A recent article [Vinkovic D, Kirman A (2006) Proc Natl Acad Sci USA 103:19261-19265] showing that the Schelling model has a physical analogue extends our understanding of the model. However, prior research has already outlined a mathematical basis for the Schelling model and simulations based on it have already enhan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clark WA,Fossett M

更新日期：2008-03-18 00:00:00

abstract：:The virulent properties of the common human and livestock pathogen Clostridium perfringens are attributable to a formidable battery of toxins. Among these are a number of large and highly modular carbohydrate-active enzymes, including the mu-toxin and sialidases, whose catalytic properties are consistent with degradat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Adams JJ,Gregg K,Bayer EA,Boraston AB,Smith SP

更新日期：2008-08-26 00:00:00

abstract：:The major Hsp70 of the mitochondrial matrix (Ssc1 in yeast) is critically important for the translocation of proteins from the cytosol, across the mitochondrial inner membrane, and into the matrix. Tim44, a peripheral inner membrane protein with limited sequence similarity to the J domain of J-type cochaperones, tethe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： D'Silva PD,Schilke B,Walter W,Andrew A,Craig EA

更新日期：2003-11-25 00:00:00

abstract：:The MAK16 gene was first defined as a gene whose mutation resulted in loss of M1 double-stranded RNA virus-like particles. The mak16-1 mutation also produces temperature-sensitive cell growth. We report here that mak16-1 cells arrest at the nonpermissive temperature in G1 phase, such that they are mating competent. We...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wickner RB

更新日期：1988-08-01 00:00:00

abstract：:Several publications have attested to the ability of herpes simplex viruses to protect cells against apoptosis. We investigated the ability of the virus to protect cells in continuous cultivation from apoptosis induced by the virus itself, and by other known inducers such as exposure to the tumor necrosis factor alpha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galvan V,Roizman B

更新日期：1998-03-31 00:00:00

abstract：:A major involvement of IFNalpha in the etiopathogenesis of systemic lupus erythematosus has been suggested by clinical observations, including the increase of serum levels of this cytokine in patients with active disease. Supporting this hypothesis, we have shown that expression of IFNalpha from a recombinant adenovir...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zagury D,Le Buanec H,Mathian A,Larcier P,Burnett R,Amoura Z,Emilie D,Peltre G,Bensussan A,Bizzini B,Gallo RC,Koutouzov S

更新日期：2009-03-31 00:00:00

abstract：:The regulatory mechanisms that use signals of low levels of reactive oxygen species (ROS) could be obscured by ROS produced under stress and thus are better investigated under homeostatic conditions. Previous studies showed that the chloroplastic atypical thioredoxin ACHT1 is oxidized by 2-Cys peroxiredoxin (2-Cys Prx...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eliyahu E,Rog I,Inbal D,Danon A

更新日期：2015-10-13 00:00:00

abstract：:The spleen from a patient with adult Gaucher's disease was shown to be deficient in a beta-glucosidase (EC 3.2.1.21) isoenzyme that has optimal activity at pH 4.0-4.3, and is stimulated by 0.02% Triton X-100. A mixture of spleen homogenates from a control and from the patient contained beta-glucosidase activity equiva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ho MW,O'Brien JS

更新日期：1971-11-01 00:00:00

abstract：:Squalene and sterol carrier protein of liver plays a general role as a vehicle for cholesterol and its water-insoluble precursors; the carrier protein is essential for enzymic cholesterol synthesis. Liver microsomal enzymes contain a small amount of endogenous carrier protein, which is readily removed by washing or pu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ritter MC,Dempsey ME

更新日期：1973-01-01 00:00:00

abstract：:During malaria and other infections, the plasma concentration of alpha 1-acid glycoprotein (AGP) increases 3- to 4-fold, but the function of this glycoprotein has been unknown. This study demonstrates, by in vitro culture of the malaria parasite Plasmodium falciparum, that the AGP concentration achieved during malaria...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedman MJ

更新日期：1983-09-01 00:00:00

abstract：:We report direct evidence for the existence of an iron(IV)-hydroxide. Resonance Raman measurements on chloroperoxidase compound II (CPO-II) reveal an isotope ((18)O and (2)H)-sensitive band at nu(Fe-O) = 565 cm(-1). Preparation of CPO-II in H(2)O using H(2)(18)O(2) results in a red-shift of 22 cm(-1), while preparatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone KL,Behan RK,Green MT

更新日期：2006-08-15 00:00:00

abstract：:A short interspersed nuclear element, Mg-SINE, was isolated and characterized from the genome of the rice blast fungus, Magnaporthe grisea. Mg-SINE was isolated as an insertion element within Pot2, an inverted-repeat transposon from M. grisea and shows typical features of a mammalian SINE. Mg-SINE is present as a 0.47...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kachroo P,Leong SA,Chattoo BB

更新日期：1995-11-21 00:00:00

abstract：:Parkin functions as a multipurpose E3 ubiquitin ligase, and Parkin loss of function is associated with both sporadic and familial Parkinson's disease (PD). We report that the Bin/Amphiphysin/Rvs (BAR) domain of protein interacting with PRKCA1 (PICK1) bound to the really interesting new gene 1 (RING1) domain of Parkin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： He J,Xia M,Yeung PKK,Li J,Li Z,Chung KK,Chung SK,Xia J

更新日期：2018-07-24 00:00:00

abstract：:ERK6, a mitogen-activated protein (MAP) kinase-related serine/threonine kinase, is highly expressed in human skeletal muscle and appears to function as a signal transducer during differentiation of myoblasts to myotubes. In transfected 293 cells, activation of the 45-kDa enzyme results in tyrosine-phosphorylated 46- a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lechner C,Zahalka MA,Giot JF,Møller NP,Ullrich A

更新日期：1996-04-30 00:00:00

abstract：:The mutagenic effect of low linear energy transfer ionizing radiation is reduced for a given dose as the dose rate (DR) is reduced to a low level, a phenomenon known as the direct DR effect. Our reanalysis of published data shows that for both somatic and germ-line mutations there is an opposite, inverse DR effect, wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vilenchik MM,Knudson AG Jr

更新日期：2000-05-09 00:00:00

abstract：:Splenic cells from one BALB/c mouse and one C57/BL mouse, immunized with purified rat liver glucocorticoid receptor (GR), were fused with the mouse myeloma cell line Sp 2/0-Ag 14. Screening for production of anti-GR-antibodies by the hybridomas was carried out with an enzyme-linked immunosorbent assay, using partially...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okret S,Wikström AC,Wrange O,Andersson B,Gustafsson JA

更新日期：1984-03-01 00:00:00

abstract：:Gene editing techniques have been extensively used to attempt to model recurrent genomic rearrangements found in tumor cells. These methods involve the induction of double-strand breaks at endogenous loci followed by the identification of breakpoint junctions within a population, which typically arise by nonhomologous...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vanoli F,Tomishima M,Feng W,Lamribet K,Babin L,Brunet E,Jasin M

更新日期：2017-04-04 00:00:00

abstract：:A potential human footprint on Western Central African rainforests before the Common Era has become the focus of an ongoing controversy. Between 3,000 y ago and 2,000 y ago, regional pollen sequences indicate a replacement of mature rainforests by a forest-savannah mosaic including pioneer trees. Although some studies...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garcin Y,Deschamps P,Ménot G,de Saulieu G,Schefuß E,Sebag D,Dupont LM,Oslisly R,Brademann B,Mbusnum KG,Onana JM,Ako AA,Epp LS,Tjallingii R,Strecker MR,Brauer A,Sachse D

更新日期：2018-03-27 00:00:00

abstract：:Worldwide, 90% of HIV-1 infections are transmitted heterosexually. Because the genital mucosa are the sites of initial contact with HIV-1 for most exposed individuals, study of the virus from the genital tract is critical for the development of vaccines and therapeutics. Previous analyses of HIV-1 in various tissues h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kemal KS,Foley B,Burger H,Anastos K,Minkoff H,Kitchen C,Philpott SM,Gao W,Robison E,Holman S,Dehner C,Beck S,Meyer WA 3rd,Landay A,Kovacs A,Bremer J,Weiser B

更新日期：2003-10-28 00:00:00

abstract：:The guanidinium group of arginine-166 has been postulated to act as an electrophilic species during phosphorylation of alkaline phosphatase. Its role could be either to stabilize the developing negative charge on the oxygen of the leaving group or the pentacoordinate transition state or to help bind the -PO2-3 group. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler-Ransohoff JE,Kendall DA,Kaiser ET

更新日期：1988-06-01 00:00:00