Abstract:
OBJECTIVES:To investigate the matrix metalloproteinases (MMPs) 2 and 9 in bladder cancer cell lines stimulated with epidermal growth factor (EGF), and to investigate the presence of gelatinases in the urine of patients with bladder tumours, in relation to the stage and grade of tumour and the EGF receptor (EGFR) status. PATIENTS, SUBJECTS AND METHODS:Conditioned media from cultured tumour cells were analysed by zymography. Urine samples from 28 patients with transitional cell carcinoma and 12 normal volunteers were also analysed. Western blotting was used to verify the bands of gelatinolytic activity. The EGFR status of the tumours was assessed by immunohistochemistry. RESULTS:MMP9 was induced by EGF in the RT112 but not the RT4 bladder tumour cell line, whereas MMP2 production was unaffected by EGF. Gelatin zymography of urine samples from patients with bladder tumours showed high levels of MMP activity, with 78% positive for MMP9 and 28% positive for MMP2. The total gelatinolytic and MMP9 activity were significantly higher in patients with high-stage invasive tumours than in those with superficial tumours (P < 0.05), and were higher than in normal controls. Gelatinolytic activity at 130 and 200 kDa in urine was identified as MMP9 and MMP2. There was no significant relationship of urinary MMP9 activity to EGFR status of the tumour. CONCLUSION:EGF induces MMP9 but not MMP2 in bladder cells. Analysis of urinary gelatinases is a useful noninvasive technique and both total gelatinase and MMP9 activity are associated with high stages of bladder tumours.
journal_name
BJU Intjournal_title
BJU internationalauthors
Nutt JE,Durkan GC,Mellon JK,Lunec Jdoi
10.1046/j.1464-410x.2003.04020.xkeywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
99-104issue
1eissn
1464-4096issn
1464-410Xpii
4020journal_volume
91pub_type
杂志文章abstract:OBJECTIVE:To report the events during and after radical cystectomy and urinary diversion for bladder cancer, in terms of major and minor complications, comparing a minimal with an extended lymphadenectomy, as more lymph nodes obtained during radical cystectomy may improve staging and thus the outcome. PATIENTS AND MET...
journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410x.2004.04557.x
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:To determine whether Thr(307)-Asn(680) and Ala(307)-Ser(680) polymorphisms of the follicle-stimulating hormone receptor (FSH-R) gene are associated with male infertility, semen quality, and reproductive hormones. PATIENTS AND METHODS:The FSH-R polymorphisms at codons 680 and 307 were analysed by restriction-...
journal_title:BJU international
pub_type: 杂志文章
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journal_title:BJU international
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doi:10.1111/j.1464-410X.2012.11230.x
更新日期:2012-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-4096.2001.01901.x
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abstract:OBJECTIVES:To investigate overall survival and prostate cancer-specific mortality in men with prostate cancer presenting with a PSA level <100 ng/mL at the time of diagnosis. PATIENTS:Five-thousand seven hundred and sixteen patients with prostate cancer and a recorded diagnostic PSA level extracted from the South Aust...
journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/bju.13411
更新日期:2016-04-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/bju.12964
更新日期:2015-04-01 00:00:00
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journal_title:BJU international
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更新日期:2007-10-01 00:00:00
abstract:OBJECTIVE:• To investigate the role of c-kit-positive interstitial cells (ICCs) in mediating muscarinic receptor-induced phasic contractions of isolated bladder strips from streptozotocin(STZ)-induced diabetic rats and to confirm the expression and location of ICCs in the rat bladder. MATERIALS AND METHODS:• Bladders ...
journal_title:BJU international
pub_type: 杂志文章
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更新日期:2011-05-01 00:00:00
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更新日期:2008-09-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,多中心研究
doi:10.1046/j.1464-410x.2001.02276.x
更新日期:2001-08-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1046/j.1464-410x.2003.04439.x
更新日期:2003-10-01 00:00:00
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journal_title:BJU international
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1046/j.1464-410x.2002.02721.x
更新日期:2002-05-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2011.10704.x
更新日期:2012-07-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,评审
doi:10.1111/j.1464-410X.2007.06930.x
更新日期:2007-07-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章,多中心研究,随机对照试验
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pub_type: 杂志文章,多中心研究
doi:10.1111/j.1464-410X.2003.04715.x
更新日期:2004-04-01 00:00:00
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更新日期:2006-08-01 00:00:00
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journal_title:BJU international
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:1999-11-01 00:00:00
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journal_title:BJU international
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doi:10.1111/j.1464-410X.2010.09652.x
更新日期:2011-05-01 00:00:00
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更新日期:2020-09-23 00:00:00
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pub_type: 杂志文章,多中心研究
doi:10.1111/bju.13307
更新日期:2016-06-01 00:00:00
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更新日期:2020-03-01 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2018-01-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2008.08232.x
更新日期:2009-05-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2004.05044.x
更新日期:2004-10-01 00:00:00
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journal_title:BJU international
pub_type: 杂志文章
doi:10.1111/j.1464-410X.2005.05851.x
更新日期:2005-12-01 00:00:00