当前位置： SCI文献检索 > INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE期刊下所有文献 > Gene expression networks underlying retinoic acid-induced differentiation of human retinoblastoma cells.

Gene expression networks underlying retinoic acid-induced differentiation of human retinoblastoma cells.

Abstract：

PURPOSE:To understand the genetic regulatory pathways underlying the retinoic acid (RA) induction of cone arrestin, gene array technology and other molecular tools were used to profile global gene expression changes in human retinoblastoma cells. METHODS:Weri-Rb-1 retinoblastoma cells were cultured in the absence or presence of RA for various periods. DNA microarray analysis profiled gene expression followed by real-time PCR and Northern and immunoblot analyses to confirm the change in expression of selected retinal genes and their gene products. Additional methodology included flow cytometry analysis, immunocytochemistry, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. RESULTS:DNA microarray analysis of approximately 6800 genes revealed RA-induced upregulation of cone-specific genes and downregulation of rod-specific genes in Weri-Rb-1 cells. Other significantly upregulated mRNAs included chicken ovalbumin upstream promoter-transcription factor (COUP-TF1), retinoid X receptor (RXR)-gamma, thyroid hormone receptor (TR)-beta2, and guanylyl cyclase-activating protein (GCAP)-1. Real-time PCR and/or Northern blot analysis confirmed the expression changes of a subset of genes including the upregulation of a pineal- and retina-specific transcription factor, CRX. RA treatment also led to G(0)/G(1) cell cycle arrest and increased both the intensity of human cone arrestin (hCAR)-immunoreactivity and the number of apoptotic cells. The cell-cycle-arrest stage correlated with the observed microarray results in which the RA treatment downregulated critical genes such as cyclins (cyclin E, cyclin D3) and cyclin-dependent kinases (CDK5, CDK10). CONCLUSIONS:These data suggest that RA induces a subpopulation of retinoblastoma cells to differentiate toward a cone cell lineage while selectively leading other cells into apoptosis.

journal_name

Invest Ophthalmol Vis Sci

journal_title

Investigative ophthalmology & visual science

authors

Li A,Zhu X,Brown B,Craft CM

doi

10.1167/iovs.02-0434

keywords:

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

996-1007

issue

3

eissn

0146-0404

issn

1552-5783

journal_volume

44

pub_type

杂志文章

相关文献

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE文献大全
  • Role of exogenous and endogenous trophic factors in the regulation of extraocular muscle strength during development.

    abstract：PURPOSE:Weak eye muscles can cause ophthalmic disorders, and in particular, strabismus. Exogenous trophic factors such as cardiotrophin (CT)-1 and insulin-like growth factor (IGF) have been shown to increase the contractile force of adult heart and skeletal muscles, respectively. In the current study, the effects of en...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.04-0393

    authors： Chen J,von Bartheld CS

    更新日期：2004-10-01 00:00:00

  • Scanning electron microscopy of normal and lased rabbit pigment epithelium.

    abstract：:Scanning electron microscopic (SEM) observations of the rabbit retinal pigment epithelium (RPE) are reported both for normal tissue and for tissue subjected to laser radiation. In normal RPE, cells were observed to be closely packed and to have the characteristic hexagonal shape. The microvilli were abundant and of tw...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Borwein B,Sanwal M,Medeiros JA,McGowan JW

    更新日期：1977-08-01 00:00:00

  • Monoallelic ABCA4 Mutations Appear Insufficient to Cause Retinopathy: A Quantitative Autofluorescence Study.

    abstract：PURPOSE:To investigate the effect of ABCA4 mutation status on lipofuscin-related quantitative autofluorescence (qAF) in humans and on bisretinoid accumulation in mice. METHODS:Genotyped parents (n = 26; age 37-64 years) of patients with biallelic ABCA4-related retinopathy underwent in-depth retinal phenotyping includi...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.15-17629

    authors： Müller PL,Gliem M,Mangold E,Bolz HJ,Finger RP,McGuinness M,Betz C,Jiang Z,Weber BH,MacLaren RE,Holz FG,Radu RA,Charbel Issa P

    更新日期：2015-12-01 00:00:00

  • The M1 muscarinic antagonist pirenzepine reduces myopia and eye enlargement in the tree shrew.

    abstract：PURPOSE:To determine the efficacy of the M1-selective muscarinic antagonist, pirenzepine, in preventing experimentally induced myopia in a mammalian model, the tree shrew. METHODS:Tree shrews were monocularly deprived (MD) using translucent goggles or negative lenses for a period of 12 days. In two of the MD groups, t...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Cottriall CL,McBrien NA

    更新日期：1996-06-01 00:00:00

  • The Antiangiogenic Effects of Gold Nanoparticles on Experimental Choroidal Neovascularization in Mice.

    abstract：Purpose:The purpose of this study was to evaluate the antiangiogenic effect of gold nanoparticles (AuNPs) on experimental choroidal neovascularization (CNV) in mice. Methods:Choroidal neovascularization was induced by rupturing the Bruch's membrane using laser photocoagulation in C57BL/6 mice. The following day, intra...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-19754

    authors： Roh YJ,Rho CR,Cho WK,Kang S

    更新日期：2016-12-01 00:00:00

  • Displaced ganglion cells in the retina of the monkey.

    abstract：:Horseradish peroxidase has been injected into the dorsal lateral geniculate nucleus of macaque monkeys to label ganglion cells of the retina by retrograde axoplasmic transport. Displaced ganglion cells, with somata in the inner nuclear layer and dendrites in the inner plexiform layer, were detected by virtue of their ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Bunt AH,Minckler DS

    更新日期：1977-01-01 00:00:00

  • Myeloid suppressor cells induced by retinal pigment epithelial cells inhibit autoreactive T-cell responses that lead to experimental autoimmune uveitis.

    abstract：PURPOSE:To test whether retinal pigment epithelial (RPE) cells are able to induce myeloid-derived suppressor cell (MDSC) differentiation from bone marrow (BM) progenitors. METHODS:BM cells were cocultured with or without RPE cells in the presence of GM-CSF and IL-4. Numbers of resultant MDSCs were assessed by flow cyt...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.11-8377

    authors： Tu Z,Li Y,Smith D,Doller C,Sugita S,Chan CC,Qian S,Fung J,Caspi RR,Lu L,Lin F

    更新日期：2012-02-23 00:00:00

  • The safety of intraocular ketorolac in rabbits.

    abstract：PURPOSE:To assess the safety of a possible substitute treatment for intraocular steroid injections, intraocular injections of ketorolac tromethamine, one of the nonsteroidal anti-inflammatory drugs, were performed in rabbits. METHODS:Either 0.5% or 0.25% preservative-free ketorolac tromethamine ophthalmic solution (0....

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.05-0764

    authors： Margalit E,Kugler LJ,Brumm MV,Meza JL,Kompella UB,Escobar ER,Christensen GR

    更新日期：2006-05-01 00:00:00

  • The Relationship Between Helicobacter pylori Infection and Open-Angle Glaucoma: A Meta-Analysis.

    abstract：PURPOSE:Previous studies assessed the associations between Helicobacter pylori infection and open-angle glaucoma (OAG) and produced inconsistent results. Therefore, we performed a meta-analysis to evaluate the effects of H. pylori infection on OAG risk. METHODS:Relevant studies that reported the associations between H...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,meta分析,评审

    doi：10.1167/iovs.15-17059

    authors： Zeng J,Liu H,Liu X,Ding C

    更新日期：2015-08-01 00:00:00

  • Choroidal blood flow regulation after posture change or isometric exercise in men with obstructive sleep apnea syndrome.

    abstract：PURPOSE:Obstructive sleep apnea (OSA) syndrome generates hypertension, atherosclerosis, and endothelial and autonomic dysfunction, which may mutually interact with ocular vascular regulation. Exercise and posture changes can be used to manipulate blood pressure, ocular perfusion pressure (OPP), or both. It was hypothes...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,随机对照试验

    doi：10.1167/iovs.11-7936

    authors： Khayi H,Pepin JL,Geiser MH,Tonini M,Tamisier R,Renard E,Baguet JP,Levy P,Romanet JP,Chiquet C

    更新日期：2011-12-16 00:00:00

  • Fundus autofluorescence in patients with leber congenital amaurosis.

    abstract：PURPOSE:Fundus autofluorescence (FAF), as an index of lipofuscin accumulation in the retinal pigment epithelium (RPE), provides indirect information on the level of metabolic activity of the RPE and thus the integrity of the RPE/photoreceptor complex. To investigate whether the photoreceptor/RPE complex is still viable...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.03-1208

    authors： Scholl HP,Chong NH,Robson AG,Holder GE,Moore AT,Bird AC

    更新日期：2004-08-01 00:00:00

  • Nighttime Driving in Older Adults: Effects of Glare and Association With Mesopic Visual Function.

    abstract：Purpose:To examine the associations between nighttime driving performance of older drivers and photopic, mesopic, and glare-based tests of visual function. Methods:Participants included 26 older drivers (71.8 ± 6.3 years), with minimal or no eye disease, but who reported vision-related nighttime driving difficulties. ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.16-21219

    authors： Kimlin JA,Black AA,Wood JM

    更新日期：2017-05-01 00:00:00

  • Nonsulfhydryl-reactive phenoxyacetic acids increase aqueous humor outflow facility.

    abstract：PURPOSE:The phenoxyacetic acid, ethacrynic acid (ECA), has potential use in glaucoma therapy because it acts to increase aqueous outflow in vivo and in vitro. In human trabecular meshwork (HTM) cell culture, ECA acts to change cell shape and attachment, effects that have been correlated with microtubule (MT) alteration...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Epstein DL,Roberts BC,Skinner LL

    更新日期：1997-07-01 00:00:00

  • Glaucoma filtration surgery using amniotic membrane transplantation.

    abstract：PURPOSE:To investigate the potential use of amniotic membrane transplantation (AMT) in the construction of glaucoma filtering blebs. METHODS:Twenty-four albino rabbits underwent glaucoma filtration surgery in one eye. In alternate cases, the conjunctival flap was replaced with AMT. Postoperative examination data were ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Barton K,Budenz DL,Khaw PT,Tseng SC

    更新日期：2001-07-01 00:00:00

  • Repeated injections of a ciliary neurotrophic factor analogue leading to long-term photoreceptor survival in hereditary retinal degeneration.

    abstract：PURPOSE:To determine whether ciliary neurotrophic factor (CNTF) or brain-derived neurotrophic factor (BDNF) treatment leads to long-term photoreceptor survival in hereditary retinal degeneration. METHODS:An autosomal dominant feline model of rod-cone dystrophy was used throughout the study with two normal animals. In ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Chong NH,Alexander RA,Waters L,Barnett KC,Bird AC,Luthert PJ

    更新日期：1999-05-01 00:00:00

  • Suppression of Blue Light at Night Ameliorates Metabolic Abnormalities by Controlling Circadian Rhythms.

    abstract：Purpose:Light-emitting diodes that emit high-intensity blue light are associated with blue-light hazard. Here, we report that blue light disturbs circadian rhythms by interfering with the clock gene in the suprachiasmatic nucleus (SCN) and that suppression of blue light at night ameliorates metabolic abnormalities by c...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 临床试验,杂志文章

    doi：10.1167/iovs.19-27195

    authors： Nagai N,Ayaki M,Yanagawa T,Hattori A,Negishi K,Mori T,Nakamura TJ,Tsubota K

    更新日期：2019-09-03 00:00:00

  • In vitro and in vivo susceptibility of Candida keratitis to topical polyenes.

    abstract：:The susceptibility of Candida albicans to topical amphotericin B and natamycin was evaluated in a model of stromal keratitis in Dutch-belted rabbits and compared with minimal inhibitory concentrations in vitro. Treatment was delayed 24 hr to allow invasive disease to occur and was then continued for 5 days. Ten strain...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： O'Day DM,Ray WA,Robinson RD,Head WS,Savage AM

    更新日期：1987-05-01 00:00:00

  • Relationship between retinal structures and retinal vessel caliber in normal adolescents.

    abstract：PURPOSE:To describe the normal physiological relationship between retinal arteriolar and venular caliber and retinal nerve fiber layer (RNFL), macular, and optic nerve head parameters. METHODS:The Sydney Childhood Eye Study assessed 2038 adolescents aged 12 years. Retinal vessel calibers were measured from digital fun...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究

    doi：10.1167/iovs.09-3878

    authors： Samarawickrama C,Huynh SC,Wang JJ,Pai A,Joachim N,Burlutsky G,Wong TY,Mitchell P

    更新日期：2009-12-01 00:00:00

  • Enzymatic disaggregation of the infected rat cornea.

    abstract：:Analysis of cell populations in the cornea may be performed rapidly and accurately employing the technique of enzymatic disaggregation. To illustrate this method normal rat corneas and corneas infected 24 and 48 hours previously with Staphylococcus aureus were disaggregated in a solution containing pancreatin and coll...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Badenoch PR,Finlay-Jones JJ,Coster DJ

    更新日期：1983-02-01 00:00:00

  • Inhibitory effects of antithrombin III against leukocyte rolling and infiltration during endotoxin-induced uveitis in rats.

    abstract：PURPOSE:This study was designed to investigate the suppressive effects of antithrombin (AT)III on inflammatory reactions during endotoxin-induced uveitis (EIU) in rats by studying leukocyte-endothelium interactions. METHODS:EIU was induced in Lewis rats by footpad injection of lipopolysaccharide (LPS). ATIII was admin...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Yamashiro K,Kiryu J,Tsujikawa A,Honjo M,Nonaka A,Miyamoto K,Honda Y,Tanihara H,Ogura Y

    更新日期：2001-06-01 00:00:00

  • Neuroendocrinimmune modulation of secretory component production by rat lacrimal, salivary, and intestinal epithelial cells.

    abstract：PURPOSE:To evaluate the kinetics, receptor specificity, molecular basis, and site selectivity of the endocrine and neural regulation of secretory component (SC) synthesis by rat lacrimal gland acinar cells. METHODS:Acinar cells from the rat lacrimal and submandibular glands, as well as epithelial cells (IEC-6) from th...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Lambert RW,Kelleher RS,Wickham LA,Vaerman JP,Sullivan DA

    更新日期：1994-03-01 00:00:00

  • Characterization of a spontaneously immortalized cell line (IOBA-NHC) from normal human conjunctiva.

    abstract：PURPOSE:To characterize a new nontransfected, spontaneously immortalized epithelial cell line from normal human conjunctiva (IOBA-NHC), both morphologically and functionally, to determine whether the differentiated phenotype of conjunctival epithelial cells is preserved. METHODS:Outgrowing cells from explanted conjunc...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.03-0560

    authors： Diebold Y,Calonge M,Enríquez de Salamanca A,Callejo S,Corrales RM,Sáez V,Siemasko KF,Stern ME

    更新日期：2003-10-01 00:00:00

  • The role of PDGF receptor inhibitors and PI3-kinase signaling in the pathogenesis of corneal neovascularization.

    abstract：PURPOSE:Corneal neovascularization remains an unsolved therapeutic problem. Platelet-derived growth factor (PDGF) is directly linked to vessel formation and stabilization. This study was undertaken to elucidate the mechanisms by which PDGF exerts its effects on corneal angiogenesis. METHODS:Corneal neovascularization ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.05-1071

    authors： Dell S,Peters S,Müther P,Kociok N,Joussen AM

    更新日期：2006-05-01 00:00:00

  • Association of ATG5 Gene Polymorphisms With Behçet's Disease and ATG10 Gene Polymorphisms With VKH Syndrome in a Chinese Han Population.

    abstract：PURPOSE:This study was conducted to explore the association of autophagy-related genes (ATGs) single nucleotide polymorphisms (SNPs) with Behçet's disease (BD) and Vogt-Koyanagi-Harada (VKH) syndrome in a Chinese Han population. METHODS:A two-stage association study was carried out in 940 BD, 1061 VKH, and 2007 health...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章,多中心研究

    doi：10.1167/iovs.15-18035

    authors： Zheng M,Yu H,Zhang L,Li H,Liu Y,Kijlstra A,Yang P

    更新日期：2015-12-01 00:00:00

  • Corneal electroretinographic function rescued by normal retinal pigment epithelial grafts in retinal degenerative Royal College of Surgeons rats.

    abstract：PURPOSE:This study was designed to examine whether retinal function can be rescued by allogeneic normal retinal pigment epithelial (RPE) grafts in Royal College of Surgeons (RCS) with retinal degeneration and, if so, whether this rescued function can be measured and followed by recording the corneal electroretinogram (...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：

    authors： Jiang LQ,Hamasaki D

    更新日期：1994-12-01 00:00:00

  • Leukocyte diapedesis in vivo induces transient loss of tight junction protein at the blood-retina barrier.

    abstract：PURPOSE:Although much is now understood about the molecular structure of tight junctions (TJs), little is known about the regulation of their function during neural inflammatory disease processes in vivo. The mechanisms by which leukocytes transmigrate the blood-retina barrier (BRB) without affecting endothelial permea...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.04-1333

    authors： Xu H,Dawson R,Crane IJ,Liversidge J

    更新日期：2005-07-01 00:00:00

  • Characterization of vitamin D production by human ocular barrier cells.

    abstract：PURPOSE:Vitamin D3 is a secosteroid mainly synthesized from the conversion of the skin precursor 7-dehydrocholesterol (7DHC) to vitamin D3 by ultraviolet (UV) B sunlight. Extrarenal synthesis of vitamin D3 has been reported in many tissues and cells, including barrier sites. This study characterizes the expression of c...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.13-13019

    authors： Alsalem JA,Patel D,Susarla R,Coca-Prados M,Bland R,Walker EA,Rauz S,Wallace GR

    更新日期：2014-04-07 00:00:00

  • Histologic effects of a new device for high-intensity focused ultrasound cyclocoagulation.

    abstract：PURPOSE:To evaluate the histologic effects and clinical outcomes of high-intensity focused ultrasound (HIFU) delivered by miniaturized annular transducers for ciliary body coagulation in an animal study. METHODS:Eighteen eyes of 18 rabbits were insonified with a ring comprising a six-sector transducer, to produce a 12...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.09-5135

    authors： Aptel F,Charrel T,Palazzi X,Chapelon JY,Denis P,Lafon C

    更新日期：2010-10-01 00:00:00

  • Automated retinal topographic maps measured with magnetic resonance imaging.

    abstract：PURPOSE:Recent studies on ocular shape have raised increased interest in the peripheral characteristics of the eye, as it potentially triggers changes in the central vision. Current techniques are, however, not capable of accurately measuring the three-dimensional shape of the retina. We describe a new magnetic resonan...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.14-15161

    authors： Beenakker JW,Shamonin DP,Webb AG,Luyten GP,Stoel BC

    更新日期：2015-01-15 00:00:00

  • Survival of donor-derived cells in human corneal transplants.

    abstract：PURPOSE:To determine the fate of donor epithelial, stromal, and endothelial cells after corneal transplantation in humans. METHODS:Fifty-two transplanted corneal buttons were explanted over a 2-year period from patients who required regrafting and had received corneas from donors of opposite sex. Fluorescence in situ ...

    journal_title：Investigative ophthalmology & visual science

    pub_type： 杂志文章

    doi：10.1167/iovs.08-2923

    authors： Lagali N,Stenevi U,Claesson M,Fagerholm P,Hanson C,Weijdegård B,Swedish Society of Corneal Surgeons.

    更新日期：2009-06-01 00:00:00