Abstract:
:Tetradecylthioacetic acid (TTA), which cannot be beta-oxidized, exerts growth-limiting properties in glioma cells. In order to investigate the importance of modulated lipid metabolism and alterations in mitochondrial properties in this cell death process, we incubated glioma cells both with TTA and the oxidizable fatty acid palmitic acid (PA), in the presence of L-carnitine and the carnitine palmitoyltransferase inhibitors etomoxir and aminocarnitine. L-carnitine partly abolished the PA-mediated growth reduction of glioma cells, whereas etomoxir and aminocarnitine enhanced the antiproliferative effect of PA. The production of acid-soluble products increased and the incorporation of PA into glycerolipids decreased after L-carnitine supplementation. L-carnitine was found to enhance the antiproliferative effect of TTA, but did not affect the incorporation of TTA into glycerolipids, or ceramide. PDMP, sphingosine 1-phosphate, desipramine, fumonisin B(1), and L-cycloserine were able not to rescue the glioma cells from PA and TTA-induced growth inhibition, suggesting that increased ceramide production is not important in the growth reduction. TTA-mediated growth inhibition was accompanied with an increased uptake of PA and increased incorporation of PA into triacylglycerol (TG). Our data suggest that mitochondrial functions are involved in fatty acid-mediated growth inhibition. Whether there is a causal relationship between TG accumulation and the apoptotic process remains to be determined.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Berge K,Tronstad KJ,Bohov P,Madsen L,Berge RKdoi
10.1194/jlr.m200312-jlr200keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
118-27issue
1eissn
0022-2275issn
1539-7262journal_volume
44pub_type
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
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journal_title:Journal of lipid research
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1194/jlr.M400335-JLR200
更新日期:2005-04-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1968-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
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更新日期:2013-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
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journal_title:Journal of lipid research
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更新日期:1977-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.E046334
更新日期:2013-12-09 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1984-06-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1977-11-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:10.1194/jlr.D600041-JLR200
更新日期:2007-02-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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doi:10.1194/jlr.M200330-JLR200
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章,评审
doi:10.1194/jlr.R800089-JLR200
更新日期:2009-04-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
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doi:10.1194/jlr.M600251-JLR200
更新日期:2007-03-01 00:00:00
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journal_title:Journal of lipid research
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00