Abstract:
:Neurofilaments (NFs) are the major intermediate filaments (IFs) of mature neurons. They play important roles in the structure and function of axons. Recently, two mutations in the neurofilament light (NFL) subunit have been identified in families affected by Charcot-Marie-Tooth (CMT) neuropathy type 2. We have characterized the effects of these NFL mutations on the formation of IF networks using a transient transfection system. Both mutations disrupted the self-assembly of human NFL. The Q333P mutant in the rod domain of NFL also disrupted the formation of rat and human NFL/NFM heteropolymers. The phenotypes produced by the P8R mutation in the head domain of NFL were less severe. The P8R mutant NFL co-polymerized with NFM to form bundled filaments and, less often, aggregates. Our results suggest that alterations in the formation of a normal IF network in neurons elicited by these NFL mutations may contribute to the development of Charcot-Marie-Tooth neuropathy.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Perez-Olle R,Leung CL,Liem RKdoi
10.1242/jcs.00148keywords:
subject
Has Abstractpub_date
2002-12-15 00:00:00pages
4937-46issue
Pt 24eissn
0021-9533issn
1477-9137journal_volume
115pub_type
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