Abstract：
:Endothelin 1 (ET-1) exerts normally a powerful vasoconstrictor role in the control of the brain microcirculation. In altered states, such as following traumatic brain injury (TBI), it may contribute to the development of ischemia and/or secondary cell injury. Because little is known of ET-1's cellular compartmentalization and its association to vulnerable neurons after TBI, we assessed its expression (both mRNA and protein) in cerebral cortex and hippocampus using correlative in situ hybridization and immunocytochemical techniques.Sprague-Dawley male rats were killed at 4, 24 or 48 h after TBI (450 g from 2 m, Marmarou's model). Semiquantitative analysis of our in situ hybridization results indicated a 2.5- and a 2.0-fold increase in ET-1 mRNA content in the hippocampus and cortex respectively which persisted up to 48 h post TBI. At 4 and 24 h after TBI enzyme-linked immunosorbent assay showed a tendency for increased ET-1 synthesis. In animals subjected to TBI, qualitative immunocytochemical analysis revealed a shift in ET-1 expression from astrocytes (in control animals) to endothelial cells, macrophages and neurons. Astrocytes and macrophages were identified unequivocally by using double immunofluorescence revealing ET-1 and glial fibrillary acidic protein or ED-1, respectively, the markers being specific for these cellular types. While this redistribution was most prominent at 4 and 24 h post TBI, at 48 h the endothelial cells remained strongly ET-1 immunopositive. The results suggest that cellular types which in the intact animal synthesize little or no ET-1 provide novel sources of the peptide after TBI. These sources may contribute to the sustained cerebrovascular hypoperfusion observed post TBI.
journal_name
Neurosciencejournal_title
Neuroscienceauthors
Petrov T,Steiner J,Braun B,Rafols JAdoi
10.1016/s0306-4522(02)00345-7keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
275-83issue
1eissn
0306-4522issn
1873-7544pii
S0306452202003457journal_volume
115pub_type
杂志文章相关文献
NEUROSCIENCE文献大全abstract：:Interactions of acoustic and somatosensory evoked potentials were studied in the anterior suprasylvian gyrus of the cat. Two kinds of interaction could be observed: occlusion or facilitation. In most cases occlusion was observed. The interactions showed dynamic changes and were susceptible to different kinds of influe...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(84)90084-8
更新日期：1984-11-01 00:00:00
abstract：:Epidemiological studies have demonstrated a relationship between advancing age and susceptibility to risk factors for median neuropathies and musculoskeletal disorders. In this study, we determined if performance of a voluntary reaching task by aged rats induced sensorimotor declines, median nerve dysfunction and incr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.07.041
更新日期：2010-10-27 00:00:00
abstract：:Long-term treatment with diazepam, a full allosteric modulator of the GABA(A) receptor, results in tolerance to its anticonvulsant effects, whereas an equipotent treatment with the partial allosteric modulator imidazenil does not produce tolerance. Use of subunit-specific antibodies linked to gold particles allowed an...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)00609-4
更新日期：1997-07-01 00:00:00
abstract：:Transparent larval zebrafish offer the opportunity to unravel genetic and neuronal networks underlying behavior in a developing system. In this study, we developed a choice chamber paradigm to measure reward-associated behavior in larval zebrafish. In the chamber where larval zebrafish have a choice of spending their ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2006.12.073
更新日期：2007-05-25 00:00:00
abstract：:In the present autoradiographic study, we took advantage of 5-hydroxytryptamine(7) (5-HT(7)) receptor knockout mice to analyze the brain distribution of 5-HT(7) receptor binding sites using [(3)H]5-carboxamidotryptamine (5-CT; a 5-HT(1A/1B/1D/5/7) receptor ligand) and [(3)H]8-hydroxy-2-(di-n-propylamino)tetralin (8-OH...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.01.014
更新日期：2004-01-01 00:00:00
abstract：:The effects of hypothermia on ischemia-reperfusion injury of the cochlea were studied in gerbils. Hearing was assessed by sequentially recording compound action potentials before, during and after the ischemia. The degree of hair cell loss in the organ of Corti was evaluated in specimens stained with rhodamine-phalloi...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(00)00510-8
更新日期：2001-01-01 00:00:00
abstract：:Neuronal activity associated with visual processing of an unfamiliar face gradually diminishes when it is viewed repeatedly. This process, known as repetition suppression (RS), is involved in the acquisition of familiarity. Current models suggest that RS results from interactions between visual information processing ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2016.03.059
更新日期：2016-06-21 00:00:00
abstract：:We have previously shown that voluntary exercise produces enhanced neurogenesis and long-term potentiation (LTP) in the dentate gyrus (DG) of mice in vitro. In the present experiments we show that rats given access to a running wheel (Runners) exhibit significantly more short-term potentiation and LTP with theta-patte...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2003.09.029
更新日期：2004-01-01 00:00:00
abstract：:Following a Pavlovian pairing procedure, alcohol-paired cues come to elicit behavioral responses that lead to alcohol consumption. Here we used an optogenetic approach to activate basolateral amygdala (BLA) axonal terminals targeting the shell of nucleus accumbens (AcbSh) and investigated a possible influence over cue...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2017.07.044
更新日期：2017-09-30 00:00:00
abstract：:Chronic restraint stress produces morphological changes in medial prefrontal cortex and disrupts a prefrontally mediated behavior, retrieval of extinction. To assess potential physiological correlates of these alterations, we compared neural activity in infralimbic and prelimbic cortex of unstressed versus stressed ra...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.10.070
更新日期：2011-02-03 00:00:00
abstract：:Lack of dystrophin in mdx mice leads to muscle fibre degeneration followed by the formation of new myofibres. This degeneration-regeneration event occurs in clusters. It is accompanied by inflammation and remodelling of the intramuscular terminal nerve fibres. Since the growth-associated protein B-50/GAP-43 has been s...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(96)83017-x
更新日期：1996-02-01 00:00:00
abstract：:Neuroinflammation contributes to neuronal death in cerebral ischemia. Urolithin A (UA), a gut microbial metabolite of ellagic acid, has emerged as a potential anti-inflammatory agent. However, its roles and precise mechanisms in stroke remain unknown. Here we found that UA treatment ameliorated infarction, neurologica...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2020.09.027
更新日期：2020-11-10 00:00:00
abstract：:Experiment 1 investigated the effects of catecholaminergic deafferentation or cell body lesions of the amygdala on fear conditioning to explicit and contextual cues. Bilateral infusions of quinolinic acid mainly damaged neurons within the basolateral region of the amygdala. 6-Hydroxydopamine infusions at the same coor...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90379-3
更新日期：1991-01-01 00:00:00
abstract：:Electroconvulsive therapy has been commonly applied in the treatment of refractory depression, but its cognitive side effects are noticed and restrict its application. The molecular mechanisms underlying the side effects remain elusive, and there is no efficient prevention. By employing a recognized electroconvulsive ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.03.060
更新日期：2010-06-30 00:00:00
abstract：:The effect of a transient increase in extracellular calcium concentration on the Schaffer collateral-commissural evoked excitatory postsynaptic potential and population spike responses of CAI pyramidal neurons was investigated using the rat in vitro hippocampal slice preparation. Brief exposure of slices (5-10 min) to...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(82)90254-8
更新日期：1982-06-01 00:00:00
abstract：:Nickel (Ni(2+)) is a toxic metal that affects the function of several ionic channels. In the N-methyl-d-aspartate (NMDA) subtype of glutamate receptor (NR), it causes activity enhancement of the channels containing the NR2B subunit and voltage-independent inhibition of those containing NR2A. Thus, it may represent a f...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.12.018
更新日期：2011-03-17 00:00:00
abstract：:Modeling Parkinson's disease remains a major challenge for preclinical researchers, as existing models fail to reliably recapitulate all of the classic features of the disease, namely, the progressive emergence of a bradykinetic motor syndrome with underlying nigrostriatal α-synuclein protein accumulation and nigrostr...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2011.12.011
更新日期：2012-02-17 00:00:00
abstract：:In the vestibular nuclei, GABAergic and glycinergic neurons play important roles in signal processing for normal function, during development, and after peripheral vestibular lesions. The chicken tangential nucleus is a major avian vestibular nucleus, whose principal cells are projection neurons with axons transmittin...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2010.11.050
更新日期：2011-02-23 00:00:00
abstract：:Our previous studies on olfactory bulbar responses in salmonid fishes suggest that pheromone signals might be processed by a mechanism distinct from that of other odorants. Using in vivo single-unit and electroencephalographic recordings, we investigated response characteristics of olfactory neurons in lake whitefish,...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(02)00717-0
更新日期：2003-01-01 00:00:00
abstract：:The acute self-stimulation of the medial forebrain bundle was reported to induce the expression of c-Fos, the protein product of c-fos, an immediate early gene, in the central nervous system. In the present study, we examined regional changes in c-Fos expression in several reward-related areas of rat brain in response...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(98)00510-7
更新日期：1999-03-01 00:00:00
abstract：:The globus pallidus, one of the basal ganglia nuclei, plays a major role in both basal ganglia physiology and pathophysiology. The globus pallidus is innervated mainly by striatal spiny neurons and globus pallidus collaterals. These GABAergic synapses constitute 90% of the input to globus pallidus cells. Despite the d...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2005.05.069
更新日期：2005-01-01 00:00:00
abstract：:Pairing vagus nerve stimulation (VNS) with movements or sounds can direct robust plasticity in motor or auditory cortex, respectively. The degree of map plasticity is influenced by the intensity and pulse width of VNS, number of VNS-event pairings, and the interval between each pairing. It is likely that these paramet...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2018.07.038
更新日期：2018-09-15 00:00:00
abstract：:The present study was designed to investigate the roles of different subtypes of opioid receptors in ventrolateral orbital cortex (VLO) opioid-evoked antinociception in formalin test by using an automatic detection system for recording the nociceptive behavior (agitation) and a manual method for detecting the duration...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.04.013
更新日期：2004-01-01 00:00:00
abstract：:Microdialysis was combined with a highly sensitive sequential multiple antigen radioimmunoassay to simultaneously measure extracellular cholecystokinin and neurotensin fragments from discrete regions of the rat brain in vivo. The assay was conducted in 96-well plates and provided a limit of detection for both peptides...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(91)90105-w
更新日期：1991-01-01 00:00:00
abstract：:The amygdaloid complex plays an essential role in auditory fear conditioning of the Pavlovian type. The available evidence suggests that the lateral nucleus is the input station of the amygdala for auditory conditioned stimuli, whereas the central medial nucleus is the output for conditioned fear responses. However, t...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/0306-4522(95)00272-k
更新日期：1995-11-01 00:00:00
abstract：:The release of chemical transmitter from nerve terminals is critically dependent on a transient increase in intracellular Ca2+. The increase in Ca2+ may be due to influx of Ca2+ from the extracellular fluid or release of Ca2+ from intracellular stores such as mitochondria. Whether Ca2+ utilized in transmitter release ...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/s0306-4522(99)00509-6
更新日期：2000-01-01 00:00:00
abstract：:Healthy aging is accompanied by neurobiological changes that affect the brain's functional organization and the individual's cognitive abilities. The aim of this study was to investigate the effect of global age-related differences in the cortical white and gray matter on neural activity in three key large-scale netwo...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2015.01.049
更新日期：2015-04-02 00:00:00
abstract：:Recently, the reports that sensory nerves contribute to induction and development of peripheral inflammation have been accumulating. Although neuropeptides have been thought to participate in modulation of inflammation, we supposed the involvement of cytokines. Interleukin-12 (IL-12) is a key regulator of cell-mediate...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2004.07.035
更新日期：2004-01-01 00:00:00
abstract：:Respiratory disturbances are a primary phenotype of the neurological disorder, Rett syndrome (RTT), caused by mutations in the X-linked gene encoding methyl-CpG-binding protein 2 (MeCP2). Mouse models generated with null mutations in Mecp2 mimic respiratory abnormalities in RTT girls. Large deletions, however, are see...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2014.02.043
更新日期：2014-05-16 00:00:00
abstract：:Riluzole, an anti-amyotrophic lateral sclerosis drug, known to decrease presynaptic glutamate release, is viewed as a candidate supplementary medication for epilepsy. In the present study, we compared the effects of riluzole and valproate (VPA) in the pilocarpine-induced limbic seizure model and in the gamma-hydroxybu...
journal_title：Neuroscience
pub_type： 杂志文章
doi：10.1016/j.neuroscience.2007.04.018
更新日期：2007-06-15 00:00:00
