Simulation of 125I induced DNA strand breaks in a CAP-DNA complex.

Abstract:

:The E. coli catabolite gene activator protein (CAP)-DNA complex with 125I located at the position of the H5 atom of the cytosine near the centre was incorporated into the PARTRAC track structure code. DNA strand breaks due to irradiation were calculated by track structure and radical attack simulations; strand breaks due to neutralisation of the highly charged 125Te ion were derived from a semi-empirical distribution. According to the calculations, the neutralisation effect dominates the strand breakage frequency at 2 bases away from the 125I decay site on both strands. The first breakage distribution counted from a 32P labelled end on the strand with 125I agreed well with experimental data, but on the opposite strand, the calculated distribution is more concentrated around the decay site and its yield is about 20% larger than the measured data.

journal_name

Radiat Prot Dosimetry

authors

Li W,Friedland W,Jacob P,Paretzke HG,Panyutin I,Neumann RD

doi

10.1093/oxfordjournals.rpd.a006738

keywords:

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

109-12

issue

1-4

eissn

0144-8420

issn

1742-3406

journal_volume

99

pub_type

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