Inactivation of the transcription factor Elf3 in mice results in dysmorphogenesis and altered differentiation of intestinal epithelium.


BACKGROUND & AIMS:The mammalian small intestine is lined by a highly specialized epithelium that functions in the digestion and absorption of nutrients. The molecular mechanisms that direct intestinal epithelial cell morphogenesis and terminal differentiation are poorly understood. We have previously identified Elf3 (E74-like factor-3) as a member of the ETS transcription factor family strongly expressed in small intestinal epithelium. The aim of this study is to investigate the biological roles of Elf3 in vivo. METHODS:Mice with a null mutation of Elf3 were generated through targeted gene disruption. Characterization of intestinal development was performed by histologic and immunohistochemical techniques. RESULTS:Targeted disruption of Elf3 resulted in fetal lethality of about 30% at around embryonic day 11.5. Seventy percent of the Elf3-deficent progeny were born and displayed severe alterations of tissue architecture in the small intestine, manifested by poor villus formation and abnormal morphogenesis and terminal differentiation of absorptive enterocytes and mucus-secreting goblet cells. Crypt cell proliferation, however, appeared intact in Elf3-deficient mice.Elf3-deficient enterocytes express markedly reduced levels of the transforming growth factor beta type II receptor (TGF-beta RII), an inducer of intestinal epithelial differentiation. CONCLUSIONS:Elf3 is an important regulator of morphogenesis and terminal differentiation of epithelial cell lineages in the small intestine.






Ng AY,Waring P,Ristevski S,Wang C,Wilson T,Pritchard M,Hertzog P,Kola I





Has Abstract


2002-05-01 00:00:00














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    pub_type: 杂志文章


    authors: Takaishi S,Cui G,Frederick DM,Carlson JE,Houghton J,Varro A,Dockray GJ,Ge Z,Whary MT,Rogers AB,Fox JG,Wang TC

    更新日期:2005-06-01 00:00:00

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    abstract:BACKGROUND & AIMS:Collagen I deposition contributes to liver fibrosis, yet little is known about other factors that mediate this process. Fibromodulin is a liver proteoglycan that regulates extracellular matrix organization and is induced by fibrogenic stimuli. We propose that fibromodulin contributes to the pathogenes...


    pub_type: 杂志文章


    authors: Mormone E,Lu Y,Ge X,Fiel MI,Nieto N

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    abstract::Abdominal cramps and urgent defecation are common side effects of clinical doses of arginine vasopressin, indicating that the drug may have stimulating effects on colonic motor activity. Four strain-gauge transducers were implanted on the colon in six monkeys. A blood flow probe was fixed on the inferior mesenteric ar...


    pub_type: 杂志文章


    authors: Zhu YR,Cowles VE,Herranz ES,Schulte WJ,Condon RE

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    pub_type: 杂志文章


    authors: Burcharth F,Jensen LI,Olesen K

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    pub_type: 杂志文章


    authors: Kodach LL,Wiercinska E,de Miranda NF,Bleuming SA,Musler AR,Peppelenbosch MP,Dekker E,van den Brink GR,van Noesel CJ,Morreau H,Hommes DW,Ten Dijke P,Offerhaus GJ,Hardwick JC

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    pub_type: 杂志文章


    authors: Maddrey WC,Cukier JO,Maglalang AC,Boitnott JK,Odell GB

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  • Symptomatic relapse after oral vancomycin therapy of antibiotic-associated pseudomembranous colitis.

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    authors: Bartlett JG,Tedesco FJ,Shull S,Lowe B,Chang T

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  • Metabolism of mixed human colonic bacteria in a continuous culture mimicking the human cecal contents.

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    pub_type: 杂志文章


    authors: Edwards CA,Duerden BI,Read NW

    更新日期:1985-06-01 00:00:00

  • Rectal sparing in antibiotic-associated pseudomembranous colitis: a prospective study.

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    pub_type: 杂志文章


    authors: Tedesco FJ,Corless JK,Brownstein RE

    更新日期:1982-12-01 00:00:00

  • Liver transplantation for hepatocellular carcinoma: a model including α-fetoprotein improves the performance of Milan criteria.

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    pub_type: 杂志文章


    authors: Duvoux C,Roudot-Thoraval F,Decaens T,Pessione F,Badran H,Piardi T,Francoz C,Compagnon P,Vanlemmens C,Dumortier J,Dharancy S,Gugenheim J,Bernard PH,Adam R,Radenne S,Muscari F,Conti F,Hardwigsen J,Pageaux GP,Chazouill

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    pub_type: 杂志文章


    authors: Isaksson G,Lundquist I,Ihse I

    更新日期:1982-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Czaja AJ,Beaver SJ,Wood JR,Klee GG,Go VL

    更新日期:1987-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Huijghebaert SM,Hofmann AF

    更新日期:1986-02-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章,多中心研究


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    authors: MacDermott RP,Bragdon MJ,Jenkins KM,Franklin GO,Shedlofsky S,Kodner IJ

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    pub_type: 杂志文章


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