Abstract:
:Skin is an attractive target for delivery of genetic therapies and vaccines. However, new approaches are needed to access this tissue more effectively. Here, we describe a new delivery technology based on arrays of structurally precise, micron-scale silicon projections, which we term microenhancer arrays (MEAs). In a human clinical study, these devices effectively breached the skin barrier, allowing direct access to the epidermis with minimal associated discomfort and skin irritation. In a mouse model, MEA-based delivery enabled topical gene transfer resulting in reporter gene activity up to 2,800-fold above topical controls. MEA-based delivery enabled topical immunization with naked plasmid DNA, inducing stronger and less variable immune responses than via needle-based injections, and reduced the number of immunizations required for full seroconversion. Together, the results provide the first in vivo use of microfabricated devices to breach the skin barrier and deliver vaccines topically, suggesting significant clinical and practical advantages over existing technologies.
journal_name
Nat Medjournal_title
Nature medicineauthors
Mikszta JA,Alarcon JB,Brittingham JM,Sutter DE,Pettis RJ,Harvey NGdoi
10.1038/nm0402-415keywords:
subject
Has Abstractpub_date
2002-04-01 00:00:00pages
415-9issue
4eissn
1078-8956issn
1546-170Xpii
nm0402-415journal_volume
8pub_type
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