Expression and kinetic changes of alkaline phosphatase and its isoenzymes in experimental rat hepatoma.

Abstract:

:AIM:To explore the expression and changes of hepatoma specific alkaline phospatase (ALP) in rats during canceration.METHODS:The ALPs and isoenzymes of rat livers and sera were investigated in SD hepatomas induced with 0.05% 2-fluoenylacetamide (2-FAA).RESULTS: By pathological examination and biochemical analysis. ALPs were overexpressed in rat livers during canceration and then were secreted into blood. Serum total ALP activities, liver ALP specific activities (U/g) including soluble and membrane-combined ALP activities of each group were all significantly higher (P < 0.01)than those of control group. The average ratios of soluble ALP to membrane-combined ALP were increased significantly after 6 weeks. ALP isoenzymes of rat sera and livers showed 5 bands on PAGE:ALP-I and ALP-II were specific for normal liver and rat hepatoma tissues, the ALP-II appeared in rat liver after 6 weeks and in sera after 8 weeks.CONCLUSION:ALP with carcino-embryonic protein was overexpressed in hepatoma tissues; the abnormal ALP-II of ALP isoenzymes in sera and liver of rats can be used as a tumor marker for early diagnosis of rat hepatoma.

journal_name

World J Gastroenterol

authors

Xiao MB,Yao DF,Zhang H,Qiang H,Huang JF,Wei Q,Jiang F

doi

10.3748/wjg.v4.i4.323

keywords:

subject

Has Abstract

pub_date

1998-08-01 00:00:00

pages

323-325

issue

4

eissn

1007-9327

issn

2219-2840

journal_volume

4

pub_type

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