Abstract:
BACKGROUND:Quantitative ELISA kits used for the measurement of indoor allergens are time-consuming. Therefore, an immunodot assay (Dustscreen) has been introduced, so that results can be obtained within 4 h. In order to assess the potential applications of the new method, we compared quantitative ELISA methods and Dustscreen. METHODS:We compared the measurements of the mite allergens Der p 1 and Der f 1, and Fel d 1 by both Dustscreen and one brand of ELISA in 82 dust samples, and we also compared the measurement of the cat allergen Fel d 1 by Dustscreen and a different brand of ELISA in 77 dust samples. Optimum dilutions of dust extracts were compared between the ELISA and the immunodot assay. The nonparametric coefficient of correlation (Spearman's rank) was calculated for each allergen. RESULTS:The immunodot assay was easy to perform. There was a good correlation between the results of the ELISA and Dustscreen (Der p 1: r=0.98, Der f 1: r=0.93, and Fel d 1: r=0.98). However, the coefficient of variation varied between 50% and 100% for Fel d 1 above 100 000 ng/g in Dustscreen. No cockroach allergen (Bla g 2) was detected in domestic dust samples in Berlin, and lower concentrations of group 2 allergen of mite (from not detectable to 19 500 ng/gm; median concentration 2000 ng/g) were found than mite group 1 allergens (sum of Der p 1 plus Der f 1: median 12 350 ng/g, minimum n.d., maximum 153 120 ng/g). CONCLUSION:Our results indicate that quantification of indoor allergens in house-dust samples can be performed easily with Dustscreen, and both the technology and methodology are satisfactory.
journal_name
Allergyjournal_title
Allergyauthors
Lau S,Schulz G,Sommerfeld C,Wahn Udoi
10.1034/j.1398-9995.2001.00117.xkeywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
993-5issue
10eissn
0105-4538issn
1398-9995pii
all117journal_volume
56pub_type
杂志文章相关文献
ALLERGY文献大全abstract:BACKGROUND:Anaphylaxis is an acute systemic allergic reaction, which can be life-threatening. H(1)-antihistamines are commonly used as an adjuvant therapy in the treatment of anaphylaxis. We sought to assess the benefits and harm of H(1)-antihistamines in the treatment of anaphylaxis. METHODS:We searched the Cochrane ...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2007.01435.x
更新日期:2007-08-01 00:00:00
abstract:BACKGROUND:Allergens from house dust mites (HDMs), Dermatophagoides pteronyssinus and Blomia tropicalis are clinically relevant in atopic respiratory diseases in tropical countries. AIMS OF THE STUDY:To evaluate immunoglobulin (Ig)E, IgG1, and IgG4 antibody responses to B. tropicalis in Brazilian atopic patients. MET...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2005.00738.x
更新日期:2005-03-01 00:00:00
abstract::There is now extensive evidence that asthma results from inflammation in large and small airways, and that the degree of inflammation reflects the clinical severity of the disease. Most of this evidence, however, has come from studies in adult patients. Evidence in children comes largely from indirect studies such as ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1999.tb04390.x
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:IgE-independent (pseudoallergic) reactions to food and food ingredients are common in a subgroup of adult patients with chronic urticaria, who have daily spontaneous occurrence of wheals. However, for children with chronic urticaria (duration longer than 6 weeks, no physical influence), no data on the import...
journal_title:Allergy
pub_type: 临床试验,杂志文章
doi:10.1111/j.1398-9995.1998.tb03817.x
更新日期:1998-11-01 00:00:00
abstract::In recent years, significant attention has been paid to the use of biotechnology to improve the quality and quantity of the food supply due in part to the projected growth in the world population, plus limited options available for increasing the amount of land under cultivation. Alterations in the food supply induced...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2005.00704.x
更新日期:2005-05-01 00:00:00
abstract:BACKGROUND:Local application of allergen extracts in specific immunotherapy is accompanied by increased compliance and significantly reduced side effects. However, efficacy of local immunotherapy in children has yet not been sufficiently demonstrated. This study was performed to determine clinical efficacy of high dose...
journal_title:Allergy
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1111/j.1398-9995.2004.00457.x
更新日期:2004-05-01 00:00:00
abstract:BACKGROUND:Genetic modification of allergenic foods such as apple has the potential to reduce their clinical allergenicity, but this has never been studied by oral challenges in allergic individuals. METHODS:We performed oral food challenges in 21 apple-allergic individuals with Elstar apples which had undergone gene ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12684
更新日期:2015-11-01 00:00:00
abstract:BACKGROUND:Intradermal skin testing of the clinically important antibiotics ciprofloxacin, clarithromycin, and rifampicin in the case of suspected allergies to antibiotics is poorly standardized. For clinical practice, standardized procedures and protocols are desired. METHODS:Fifteen healthy volunteers were tested wi...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2012.02807.x
更新日期:2012-05-01 00:00:00
abstract::The efficacy of single-allergen-specific immunotherapy in polysensitized subjects is a matter of debate. We therefore performed a post hoc analysis of pooled data from six randomized, double-blind, placebo-controlled trials (N = 1871) comparing the efficacy and safety of the SQ-standardized grass allergy immunotherapy...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12074
更新日期:2013-02-01 00:00:00
abstract::House dust mite D. farinae and Der f II-specific IgG and IgG subclass responses were evaluated in 32 adults with perennial rhinitis undergoing immunotherapy for 1 year by means of IgG-RAST and ELISA. The ELISA method, which is based on subclass-specific monoclonal antibodies, could detect 0.5-1.5 ng/ml of specific ant...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1992.tb05092.x
更新日期:1992-04-01 00:00:00
abstract::The cellular immunity to Yersinia enterocolitica serotype 3 and crude human thyroid extract in 64 patients with thyroid diseases and 25 controls was studied by the leucocyte migration test. In the patient group as a whole and in patients with Graves' disease and nontoxic diffuse goitre a significantly reduced leucocyt...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1978.tb01513.x
更新日期:1978-04-01 00:00:00
abstract:BACKGROUND:Food allergy in children can be life-threatening. Component-resolved diagnostics approach to food anaphylaxis is rarely assessed in children. The aim of the study was to identify the food allergen components as the triggers responsible for severe anaphylaxis, with regard to characteristics and associated ris...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13810
更新日期:2019-10-01 00:00:00
abstract::A 60-year-old man developed an anaphylactic reaction immediately after administration of intramuscular sodium cefuroxime. By means of oral controlled challenge, the reaction was reproduced with a smaller dose of cefuroxime axetil. Cross-reactivity with other betalactam antibiotics was ruled out by in vivo tests. This ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1995.tb01160.x
更新日期:1995-04-01 00:00:00
abstract::Mast cell degranulation, and the subsequent recruitment of infiltrating inflammatory cells, such as eosinophils, into the nasal mucosa has long been considered the most important model to explain allergic rhinitis. Several studies show a decrease in the number of eosinophils and possibly also mast cells during local c...
journal_title:Allergy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1398-9995.1995.tb02737.x
更新日期:1995-01-01 00:00:00
abstract::It is suggested that eosinophils (Eos) play an important role in the pathogenesis of bronchial asthma by releasing cytotoxic cationic eosinophil granule proteins and damaging bronchial epithelial cells. However, the exact nature of the actual inducer of eosinophil degranulation in vivo is unclear. We examined eosinoph...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1996.tb04669.x
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:The pollen-food syndrome (PFS) is an association of food allergies to fruits, nuts, and vegetables in patients with pollen allergy. Mal d 1, the major apple allergen, is one of the most commonly associated food allergens for birch pollen-allergic patients suffering from PFS. Although the reactions are due to...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2008.01844.x
更新日期:2009-04-01 00:00:00
abstract:BACKGROUND:Associations between vitamin D status and childhood asthma are increasingly reported, but direct causation and mechanisms underlying an effect remain unknown. We investigated the effect of early-life vitamin D deficiency on the development of murine neonatal allergic airways disease (AAD). METHODS:In utero ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12465
更新日期:2014-10-01 00:00:00
abstract::We investigated the relationship between residence in the neighbourhood of an aluminium smelter and the prevalence of atopy in schoolchildren (7-13 years of age). Atopy was assessed in 556 of the 620 participants by a skin prick test with eight common aeroallergens. The median exposures to sulphur dioxide and fluoride...
journal_title:Allergy
pub_type: 杂志文章
doi:
更新日期:1996-10-01 00:00:00
abstract:BACKGROUND:New therapies are necessary to address inadequate asthma control in many patients. This study sets out to investigate whether hypoxia-inducible factor (HIF) is essential for development of allergic airway inflammation (AAI) and therefore a potential novel target for asthma treatment. METHODS:Mice conditiona...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2011.02594.x
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:Serum immunoglobulin E (IgE) level is recognized to be under strong genetic control, but the causal and susceptibility genes remain to be identified. We sought to investigate the association between single nucleotide polymorphisms (SNPs) in the Toll-like receptor (TLR) signaling pathway and total serum IgE l...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2008.01889.x
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:The prevalence of allergic rhinoconjunctivitis has increased significantly over the past decades with grass pollen being a common trigger. The impact of allergy on patient's quality of life is substantial. AIM:To investigate the sustained effect on quality of life during the grass pollen season 1 year after...
journal_title:Allergy
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1398-9995.2009.02238.x
更新日期:2010-06-01 00:00:00
abstract:BACKGROUND:Children with spina bifida (SB) are exposed to latex soon after birth during bladder catheterization, rectal disimpaction, and multiple surgical procedures. IgE-mediated latex-allergic reactions have been reported recently in these children. Our study was designed to assess the prevalence of allergic reactio...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.1999.00683.x
更新日期:1999-01-01 00:00:00
abstract::: The use of specific immunotherapy (SIT) for allergic disorders has recently been extended by introduction of a convenient, tablet-based, disease-modifying vaccine against grass pollen allergy. Allergy immunotherapy tablet (AIT) programmes targeting house dust mite and other allergies are currently in late-phase deve...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2011.02644.x
更新日期:2011-07-01 00:00:00
abstract:BACKGROUND:Mustard IgE-mediated allergy is supposed to be a rare cause of food allergy, and its clinical features and cross-reactivities have not been fully elucidated. METHODS:A prospective study was carried out, recruiting mustard allergic patients, and paired control subjects. A clinical questionnaire was administe...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2005.00644.x
更新日期:2005-01-01 00:00:00
abstract::A safe food supply is a legitimate expectation of consumers, which the food industry must meet. Allergic reactions to food are recognised as a significant public health problem. Protecting allergic consumers against such reactions, while minimising the impact of food allergy on their quality of life, poses a challenge...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2001.00928.x
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Dysphagia is the main symptom of adult eosinophilic esophagitis (EoE). We describe a novel syndrome, referred to as "food-induced immediate response of the esophagus" (FIRE), observed in EoE patients. METHODS:Food-induced immediate response of the esophagus is an unpleasant/painful sensation, unrelated to d...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14495
更新日期:2021-01-01 00:00:00
abstract::Application of strict diagnostic criteria to celiac disease has led to the realization that there is a wide clinical spectrum within this disease. Normally there are immune reactions to dietary proteins, particularly secretory antibodies of IgA class, low titers of serum antibody and specific down-regulation of IgE an...
journal_title:Allergy
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract:BACKGROUND:Adolescence represents a vulnerable time for individuals with asthma and allergic conditions. They suffer an unexpected degree of morbidity. This systematic review aimed to understand the challenges faced by adolescents and young adults with these conditions. METHODS:A systematic literature search was under...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14258
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND AND AIMS:Aspirin, a cyclo-oxygenase (COX)-1 and COX-2 inhibitor, is the antiplatelet drug of choice to prevent serious vascular events. Adverse reactions to aspirin are frequent particularly among patients with asthma, chronic rhinosinusitis and nasal polyps. COX-1 inhibitors but not COX-2 inhibitors precipi...
journal_title:Allergy
pub_type: 杂志文章,随机对照试验
doi:10.1111/j.1398-9995.2007.01473.x
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND:Pollutants may affect pollen allergenicity and thus the prevalence of allergies. Although a few studies are available in literature, the connection between pollution and the allergenic potential of pollen has yet to be clearly defined. The objective of this study was to evaluate the effect of traffic-related...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2012.02846.x
更新日期:2012-07-01 00:00:00