Isopropyl unoprostone increases the activities of matrix metalloproteinases in cultured monkey ciliary muscle cells.

Abstract:

PURPOSE:The mechanism by which the prostaglandin F2alpha-related antiglaucoma compound isopropyl unoprostone (referred to as unoprostone) reduces intraocular pressure is largely unknown. Another prostaglandin F2alpha-related compound, latanoprost, influences the activities of matrix metalloproteinases in ciliary muscle. Unoprostone ophthalmic solution is metabolized to oxidized metabolites, mainly M1 and M2, in the eye. The aim of this study was to investigate whether intraocular metabolites of unoprostone, M1 and M2, change the metalloproteinase activity in cultured monkey ciliary muscle cells. MATERIALS AND METHODS:Monkey ciliary muscle cells and trabecular meshwork cells were grown separately to confluence in monolayer cell cultures. M1 (10 nM, 100 nM, or 1 microM), M2 (10 nM, 100 nM, or 1 microM), 100 nM prostaglandin F2alpha, or vehicle solutions were added to each culture medium for 48 hours. The media were then assayed to measure metalloproteinase activities quantitatively by means of substrate zymography. RESULTS:Compared with the vehicle controls, M1, M2, and prostaglandin F2alpha significantly increased the metalloproteinase-2 activity in cultured ciliary muscle cells in a dose-dependent manner, but did not affect the metalloproteinase-2 activity in cultured trabecular meshwork cells. All experimented prostaglandins slightly increased metalloproteinase-9 activity in ciliary muscle cells, although these changes were not significant. CONCLUSIONS:The current results show that unoprostone influences the metabolism of the extracellular matrix in the ciliary muscle and that remodeling of the extracellular matrix in the ciliary muscle may be a possible mechanism by which unoprostone ophthalmic solution reduces intraocular pressure.

journal_name

J Glaucoma

journal_title

Journal of glaucoma

authors

Kashiwagi K,Jin M,Suzuki M,Tanaka Y,Iizuka Y,Tsukahara S

doi

10.1097/00061198-200108000-00005

keywords:

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

271-6

issue

4

eissn

1057-0829

issn

1536-481X

journal_volume

10

pub_type

杂志文章
  • Meibomian Gland Features and Conjunctival Goblet Cell Density in Glaucomatous Patients Controlled With Prostaglandin/Timolol Fixed Combinations: A Case Control, Cross-sectional Study.

    abstract:PURPOSE:To investigate, using in vivo confocal microscopy (IVCM), the Meibomian gland (MG) features and conjunctival goblet cell density (GCD) in glaucomatous patients controlled with prostaglandin/timolol fixed combinations (PTFCs). MATERIALS AND METHODS:In this cross-sectional study, 60 white patients were treated w...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000000899

    authors: Agnifili L,Mastropasqua R,Fasanella V,Brescia L,Scatena B,Oddone F,Mastropasqua L

    更新日期:2018-04-01 00:00:00

  • Clinical Evaluation of Methods to Correct Intraocular Pressure Measurements by the Goldmann Applanation Tonometer, Ocular Response Analyzer, and Corvis ST Tonometer for the Effects of Corneal Stiffness Parameters.

    abstract:PURPOSE:To evaluate the effectiveness of methods to correct intraocular pressure (IOP) measurements obtained using the Goldmann applanation tonometer (GAT), the ocular response analyzer (ORA), and the Corvis ST tonometer (CVS) for the effects of corneal stiffness parameters: central corneal thickness (CCT), corneal cur...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000000359

    authors: Bao F,Huang Z,Huang J,Wang J,Deng M,Li L,Yu A,Wang Q,Elsheikh A

    更新日期:2016-06-01 00:00:00

  • Effect of Angle of Incidence on Anterior Chamber Angle Metrics From Optical Coherence Tomography.

    abstract:PURPOSE:To evaluate the local variability of anterior chamber angle (ACA) metrics obtained by time domain (TD) and spectral domain (SD) optical coherence tomography (OCT). MATERIALS AND METHODS:Anterior-segment OCT imaging was performed on 30 normal eyes using Visante TD-OCT and 40 normal eyes using Cirrus SD-OCT. For...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000000169

    authors: Pan X,Maram J,Marion K,Dastiridou A,Zhang ZY,Dominguez-Velasco MF,Sadda SR,Chopra V

    更新日期:2016-01-01 00:00:00

  • Progressive High Hypermetropic Shift as a Refractive Surprise Following Glaucoma Filtration Surgery in a Phakic Child With Early-Onset Childhood Glaucoma Associated With Axenfeld-Rieger Anomaly.

    abstract:PURPOSE:We report a case of progressive high hypermetropia following glaucoma filtration surgery in a child with Axenfeld-Rieger syndrome and congenital glaucoma. METHODS/RESULTS:We report a case of a 4-day-old female child presented as a case of Axenfeld-Rieger anomaly with secondary glaucoma in both eyes (OU), who u...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001283

    authors: Kumar P,Senthil S

    更新日期:2019-08-01 00:00:00

  • Clinical Presentation and Management Outcomes of Coexistent Congenital Glaucoma and Retinopathy of Prematurity.

    abstract:PURPOSE:The purpose of this study was to report presentation and outcomes of coexisting congenital glaucoma with retinopathy of prematurity (ROP) in "Indian Twin cities ROP study (ITCROPS)." METHODS:Children with ROP and congenital glaucoma were identified from ITCROPS (prospective digital database) between 1997 and 2...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001124

    authors: Senthil S,Balijepalli P,Garudadri C,Jalali S

    更新日期:2019-01-01 00:00:00

  • Deep Sclerectomy With a New Nonabsorbable Uveoscleral Implant (Esnoper-Clip): 1-Year Outcomes.

    abstract:PURPOSE:To report the safety and the effectiveness of deep sclerectomy (DS) with a new nonabsorbable uveoscleral hema implant (Esnoper-Clip) designed to increase trabecular and uveoscleral outflow and to achieve higher intrascleral blebs. MATERIALS AND METHODS:Twenty-seven eyes of 27 patients with open-angle glaucoma,...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000000253

    authors: Loscos-Arenas J,Parera-Arranz A,Romera-Romera P,Castellvi-Manent J,Sabala-Llopart A,de la Cámara-Hermoso J

    更新日期:2015-08-01 00:00:00

  • Relation between office intraocular pressure and 24-hour intraocular pressure in patients with primary open-angle glaucoma treated with a combination of topical antiglaucoma eye drops.

    abstract:PURPOSE:To determine the relation between office intraocular pressure (IOP) and 24-hour IOP in patients with primary open-angle glaucoma (POAG) treated with 3 kinds of antiglaucoma eye drops. PATIENTS AND METHODS:Subjects were 42 patients with POAG (71 eyes). All were being treated with 3 different topical antiglaucom...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e31802ff85f

    authors: Nakakura S,Nomura Y,Ataka S,Shiraki K

    更新日期:2007-03-01 00:00:00

  • Effects of Nonporous Silica Nanoparticles on Human Trabecular Meshwork Cells.

    abstract:PRECIS:Silica nanoparticles (SiNPs), which are potential drug carriers for glaucoma treatment, may induce mild dose-dependent cytotoxicity but not so severe as to compromise a mammalian target of rapamycin (mTOR) pathway in immortalized trabecular meshwork (TM) cells. PURPOSE:Nanoparticle-based ophthalmic drug deliver...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001709

    authors: Kim M,Park JH,Jeong H,Hong J,Park CY

    更新日期:2021-02-01 00:00:00

  • Scleral indentation optimizes visualization of anterior chamber angle during goniosynechialysis.

    abstract:PURPOSE:To propose a new surgical technique for optimized visualization of the chamber angle using scleral indentation under an operating microscope in goniosynechialysis (GSL). PATIENTS AND METHODS:Six volunteers with normal chamber angle and five patients with synechial angle closure glaucoma were examined. Scleral ...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/01.ijg.0000170061.51878.02

    authors: Takanashi T,Masuda H,Tanito M,Nonoyama S,Katsube T,Ohira A

    更新日期:2005-08-01 00:00:00

  • Sensitivity and specificity of new GDx parameters.

    abstract:PURPOSE:The GDx is a scanning laser polarimeter that assesses peripapillary nerve fiber layer thickness. In addition to the 14 existing outcome parameters, four new parameters have been described recently: the Ellipse Standard Deviation (ESD), the Normalized Superior Area (NSA), the Normalized Inferior Area (NIA) and t...

    journal_title:Journal of glaucoma

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1097/00061198-200402000-00006

    authors: Colen TP,Tang NE,Mulder PG,Lemij HG

    更新日期:2004-02-01 00:00:00

  • A Comparative Analysis of Goldmann Tonometry Correction.

    abstract:PURPOSE:The measurement of intraocular pressure (IOP) by Goldmann applanation tonometry (GAT) is based on assumptions about corneal parameters. To correct for variations in corneal curvature and thickness, a number of equations have been proposed. This study evaluates the in vivo accuracy of these equations from indivi...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000000588

    authors: Asejczyk-Widlicka M,Srodka W,Pierscionek BK

    更新日期:2017-03-01 00:00:00

  • Comparison of ICare rebound tonometer with noncontact tonometer in healthy children.

    abstract:PURPOSE:To evaluate the effectiveness of using a noncontact tonometer (NCT) versus a rebound tonometer (ICare) when measuring the intraocular pressure (IOP) in healthy children. DESIGN:Prospective observational study. METHODS:A total of 180 (96 males and 84 females) healthy children, aged 6 months to 15 years, were r...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e3181d12dc4

    authors: Kageyama M,Hirooka K,Baba T,Shiraga F

    更新日期:2011-01-01 00:00:00

  • Preference-based Glaucoma-specific Health-related Quality of Life Instrument: Development of the Health Utility for Glaucoma.

    abstract:PURPOSE:To develop a descriptive system for a glaucoma-specific preference-based health-related quality of life (HRQoL) instrument: the Health Utility for Glaucoma (HUG-5). METHODS:The descriptive system was developed in 2 stages: item identification and item selection. A systematic literature review of HRQoL assessme...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000000984

    authors: Muratov S,Podbielski DW,Kennedy K,Jack SM,Pemberton J,Ahmed IIK,Baltaziak M,Xie F

    更新日期:2018-07-01 00:00:00

  • Preliminary Report on a Novel Virtual Reality Perimeter Compared With Standard Automated Perimetry.

    abstract:PRECIS:The VisuALL head-mounted perimetry in normal subjects and glaucoma patients had a moderate to strong correlation with the Humphrey Field Analyzer (HFA). PURPOSE:Visual field testing has a vital role in diagnosing and managing glaucoma. The current clinical practice relies on large, table-based testing units. Th...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001670

    authors: Razeghinejad R,Gonzalez-Garcia A,Myers JS,Katz LJ

    更新日期:2021-01-01 00:00:00

  • Longitudinal Changes in Bleb Height, Vascularity, and Conjunctival Microcysts After Trabeculectomy.

    abstract:OBJECTIVE:The objective of this article was to study longitudinal changes in bleb morphology (presence of microcysts and bleb height and vascularity) in subjects enrolled in the Singapore 5-Fluorouracil (5-FU) trabeculectomy study. STUDY DESIGN:Retrospective subgroup analysis of a randomized controlled trial. MATERIA...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/IJG.0000000000000967

    authors: Kumaran A,Husain R,Htoon HM,Aung T

    更新日期:2018-07-01 00:00:00

  • Floppy eyelid syndrome as an indicator of the presence of glaucoma in patients with obstructive sleep apnea.

    abstract:PURPOSE:The aim of the study was to investigate whether floppy eyelid syndrome (FES) could be an indicator of glaucoma in patients with obstructive sleep apnea (OSA). MATERIALS AND METHODS:A total of 152 patients were included: 75 patients with OSA and without FES; 52 patients with OSA and FES; and 25 non-OSA patients...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e31829da19f

    authors: Muniesa M,Sánchez-de-la-Torre M,Huerva V,Lumbierres M,Barbé F

    更新日期:2014-01-01 00:00:00

  • Scanning protocol choice affects optical coherence tomography (OCT-3) measurements.

    abstract:PURPOSE:To determine the optimal OCT-3 scanning protocol. METHODS:Normal subjects, ocular hypertensive, and open-angle glaucoma patients were enrolled. The "fast" RNFL scan protocol consists of 3 consecutive circular scans, each containing 256 A-scans obtained in a single 1.92 second session. The "regular" RNFL scan p...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/00061198-200404000-00010

    authors: Zafar S,Gurses-Ozden R,Makornwattana M,Vessani R,Liebmann JM,Tello C,Ritch R

    更新日期:2004-04-01 00:00:00

  • Using the early postoperative intraocular pressure to predict pressure control after a trabeculectomy.

    abstract:PURPOSE:The goal of this study was to identify the early postoperative intraocular pressure (IOP) that predicts low pressure control after a trabeculectomy with mitomycin C. MATERIALS AND METHODS:This study retrospectively analyzed the medical records of the initial trabeculectomy with mitomycin C in patients with pri...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e318285fd7d

    authors: Okimoto S,Kiuchi Y,Akita T,Tanaka J

    更新日期:2014-08-01 00:00:00

  • Micropulse Trans-scleral Cyclophotocoagulation in Patients With Glaucoma: 1- and 2-Year Treatment Outcomes.

    abstract::PRéCIS:: Micropulse trans-scleral cyclophotocoagulation (TSCPC) is an effective and safe IOP-lowering treatment for patients with primary or secondary glaucoma. PURPOSE:To investigate the 1-year and 2-year effect on intraocular pressure (IOP) and safety profile of micropulse TSCPC in patients with glaucoma. METHODS:...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001552

    authors: de Crom RMPC,Slangen CGMM,Kujovic-Aleksov S,Webers CAB,Berendschot TTJM,Beckers HJM

    更新日期:2020-09-01 00:00:00

  • Evaluation of macular thickness and peripapillary retinal nerve fiber layer thickness for detection of early glaucoma using spectral domain optical coherence tomography.

    abstract:PURPOSE:To evaluate the diagnostic ability of macular parameters and peripapillary retinal nerve fiber layer (RNFL) parameters for early glaucoma using spectral domain optical coherence tomography (SD-OCT). MATERIALS AND METHODS:One eye from 32 early glaucoma patients (including preperimetric glaucoma) and 32 normal p...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e3181e079ed

    authors: Nakatani Y,Higashide T,Ohkubo S,Takeda H,Sugiyama K

    更新日期:2011-04-01 00:00:00

  • Cohort Study of Nonmelanoma Skin Cancer and the Risk of Exfoliation Glaucoma.

    abstract:PRECIS:In a cohort study of 120,307 participants with 25+ years of follow-up, a history of nonmelanoma skin cancer (NMSC) was associated with a 40% higher exfoliation glaucoma (XFG) risk. PURPOSE:The purpose of this study was to evaluate the relationship between NMSC (a marker of ultraviolet radiation exposure) and XF...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001496

    authors: Kang JH,VoPham T,Laden F,Rosner BA,Wirostko B,Ritch R,Wiggs JL,Qureshi A,Nan H,Pasquale LR

    更新日期:2020-06-01 00:00:00

  • Imaging of descemet membrane detachment after trabeculectomy using slit-lamp-adapted optical coherence tomography.

    abstract:PURPOSE:To report the use of slit-lamp-adapted optical coherence tomography (SL-OCT) in imaging 2 patients with Descemet membrane detachment following anterior chamber reformation after trabeculectomy. METHODS:Two patients, with leaking and overfiltering blebs, respectively, developed Descemet detachments after reform...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e31819c469e

    authors: Palmiero PM,Sbeity Z,Liebmann J,Ritch R

    更新日期:2009-12-01 00:00:00

  • Comparison of scanning laser polarimetry and optical coherence tomography in quantitative retinal nerve fiber assessment.

    abstract:PURPOSE:To investigate the relationship between retinal nerve fiber layer (RNFL) measurements in corresponding areas obtained with scanning laser polarimetry and optical coherence tomography and to compare their discriminating ability in the diagnosis of preperimetric and perimetric glaucoma. PATIENTS AND METHODS:Thre...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e3181a2fc0e

    authors: Schrems WA,Mardin CY,Horn FK,Juenemann AG,Laemmer R

    更新日期:2010-02-01 00:00:00

  • Detecting early functional damage in glaucoma suspect and ocular hypertensive patients with the multifocal VEP technique.

    abstract:PURPOSE:To determine whether the multifocal visual evoked potential (mfVEP) technique can detect early functional damage in ocular hypertensive (OHT) and glaucoma suspect (GS) patients with normal standard achromatic automated perimetry (SAP) results. PATIENTS AND METHODS:Twenty-five GS patients (25 eyes), 25 patients...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/01.ijg.0000212237.26466.0e

    authors: Thienprasiddhi P,Greenstein VC,Chu DH,Xu L,Liebmann JM,Ritch R,Hood DC

    更新日期:2006-08-01 00:00:00

  • Stratus OCT in dominant optic atrophy: features differentiating it from glaucoma.

    abstract:PURPOSE:To describe the Stratus optical coherence tomography (OCT) retinal nerve fiber layer (RNFL) thickness profile of dominant optic atrophy (DOA), to differentiate it from that of glaucoma. METHODS:RNFL thickness was measured using Stratus OCT in 32 eyes of 16 DOA patients. RESULTS:The temporal-most clock hour me...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0b013e31804d23aa

    authors: Kim TW,Hwang JM

    更新日期:2007-12-01 00:00:00

  • The Chinese Glaucoma Study Consortium for Patients With Glaucoma: Design, Rationale and Baseline Patient Characteristics.

    abstract:PRECIS:Chinese Glaucoma Study Consortium (CGSC) as the first nationwide glaucoma registry in China, we describe its design, rationale, the geographic distribution of the hospitals, and baseline patient characteristics. AIM:As a leading cause of blindness in China, glaucoma affects 2% to 4% of adults over the age of 40...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001378

    authors: Zhang H,Jia H,Duan X,Li L,Wang H,Wu J,Hu J,Cao K,Zhao A,Liang J,Song J,Qiao C,Wang N,Investigators for The Chinese Glaucoma Study Consortium Investigators.

    更新日期:2019-11-01 00:00:00

  • Impact of Phacoemulsification Combined with XEN Gel Stent Implantation on Corneal Endothelial Cell Density: 2-Year Results.

    abstract:INTRODUCTION:Corneal integrity has long been a preoccupation of glaucoma surgeons considering glaucoma drainage device surgery or antimetabolite-enhanced trabeculectomy. Despite having demonstrated a good safety profile and significant intraocular pressure-lowering capacities, the impact of XEN gel stents on endothelia...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001430

    authors: Gillmann K,Bravetti GE,Rao HL,Mermoud A,Mansouri K

    更新日期:2020-03-01 00:00:00

  • Relationship Between Serum Glucose Levels and Intraocular Pressure, a Population-based Cross-sectional Study.

    abstract:PURPOSE:The purpose of this study is to assess the relationship between serum glucose levels and intraocular pressure (IOP) both in subjects with impaired fasting glucose (IFG) as well as in subjects with diabetes mellitus (DM). MATERIALS AND METHODS:Data of a cross-sectional sample of 18,406 subjects who underwent ro...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000000700

    authors: Cohen E,Kramer M,Shochat T,Goldberg E,Krause I

    更新日期:2017-07-01 00:00:00

  • Acute Angle Closure Following Implantable Collamer Lens for Myopia.

    abstract::We report a case of acute angle closure with significantly elevated intraocular pressure 9 hours after implantation of a phakic intraocular lens for high myopia. ...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001533

    authors: Strungaru MH,González Rodríguez J,Weisbrod DJ,Tayfour F,Buys YM

    更新日期:2020-07-01 00:00:00

  • Comparison of Ab Externo and Ab Interno 360-degree Suture Trabeculotomy in Adult Open-angle Glaucoma.

    abstract:PRECIS:Ab interno (gonioscopy-assisted transluminal trabeculotomy) and ab externo 360-degree suture trabeculotomy (ST) achieved similar success rates for reducing intraocular pressure (IOP) without serious complications threatening visual acuity. PURPOSE:We aimed to compare the efficacy of ab externo and ab interno 36...

    journal_title:Journal of glaucoma

    pub_type: 杂志文章

    doi:10.1097/IJG.0000000000001627

    authors: Yalinbas D,Dilekmen N,Hepsen IF

    更新日期:2020-11-01 00:00:00