Validation and casework application of a Y chromosome specific STR multiplex.

Abstract:

:A series of validation experiments was performed for a Y chromosome specific STR multiplex system following the suggestions made by the Technical Working Group DNA Analysis Methods (TWGDAM). The multiplex PCR products were detected on Perkin-Elmer 373 and 377 automated sequencers using two labeling colors. No problems regarding the stability, robustness and sensitivity of the Y STR multiplex were observed. Mixture studies revealed a cut off rate similar to autosomal STRs for mixtures of male DNAs and no interference of any female admixture. The comparison of the Y STR results to the autosomal typing results for 56 nonprobative semen stains and swabs, showed a slightly higher success rate in detecting the semen donor's alleles for the Y STR multiplex. Two examples are shown to illustrate the usefulness of Y STR typing for DNA mixtures. In one case the Y STR results confirmed an isolated exclusion; in the other case, the interpretation of a mixture was clarified since the Y STR results proved the presence of DNA from at least two semen donors. Y STR typing is a valuable addition to the forensic DNA testing panel.

journal_name

Forensic Sci Int

authors

Prinz M,Ishii A,Coleman A,Baum HJ,Shaler RC

doi

10.1016/s0379-0738(00)00467-9

keywords:

subject

Has Abstract

pub_date

2001-09-01 00:00:00

pages

177-88

issue

3

eissn

0379-0738

issn

1872-6283

pii

S0379073800004679

journal_volume

120

pub_type

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