Mobility shift assay of calcium-binding proteins of mouse epididymal spermatozoa.

Abstract:

:The calcium-binding proteins (CBPs) of mouse epididymal spermatozoa were analysed by mobility changes in the presence of added Ca2+ in two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The expression patterns of relatively high molecular weight CBPs (Mr > 20 kDa) were different between caput and cauda epididymal spermatozoa. There was a constitutive expression of low molecular weight CBPs (Mr < 20 kDa) regardless of the epididymal region. Most of the CBPs disappeared after the acrosome reaction (AR) induced by Ca2+ ionophore A23187, suggesting that they originated from the acrosome and/or the plasma membrane overlaying the acrosome. Taken together, it can be suggested that changes in CBPs of spermatozoa are important features of sperm maturation during epididymal transit, and that they may be related to the fertilizing ability of mouse epididymal spermatozoa.

journal_name

Andrologia

journal_title

Andrologia

authors

Gye MC,Park S,Kim YS,Ahn HS

doi

10.1046/j.1439-0272.2001.00419.x

keywords:

subject

Has Abstract

pub_date

2001-07-01 00:00:00

pages

193-8

issue

4

eissn

0303-4569

issn

1439-0272

pii

and419

journal_volume

33

pub_type

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