On the capacity of mouse spermatozoa for spontaneous acrosome reaction in the male and female genital tract.

Abstract:

:The spontaneous acrosomal reaction of mouse spermatozoa during their passage through the male and female genital tract was studied by indirect immunofluorescence staining techniques using antibodies against the outer acrosomal membrane (OAM). Low rates of spontaneously acrosome-reacted spermatozoa could be observed during the passage through the male reproductive tract, namely 4% in the testis (SD +/- 0.8%) and in the caput epididymidis (SD +/- 1.5%), and up to 8% in the cauda epididymidis (SD +/- 0.5%) and in the ductus deferens (SD +/- 0.5%), whereas a higher value of 13% (SD +/- 1%) of reacted spermatozoa was obtained from the corpus epididymidis. Spermatozoa removed from the uterus 20 min up to 14 h post copulation (p.c.) exhibited a constant degree of 8% (SD +/- 6%) acrosomal reaction, whereas 43% (SD +/- 6%) of the oviductal sperms were acrosome-reacted, independent of the time of removal p.c. Rates of spontaneously acrosome-reacted spermatozoa from the male and female reproductive tract could be increased by 2-fold and up to 7-fold by an in vitro incubation in modified Tyrode's medium for 210 min. On the basis of our results wer postulate a basic level of spontaneously acrosome-reacted spermatozoa in the oviduct at ovulation time, which might be relevant to ensure the environmental conditions for the fertilization process. Additionally, we offer a possibility of optimizing in vitro fertilization techniques.

journal_name

Andrologia

journal_title

Andrologia

authors

Klemm M,Engel W

doi

10.1111/j.1439-0272.1991.tb02592.x

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

427-33

issue

6

eissn

0303-4569

issn

1439-0272

journal_volume

23

pub_type

杂志文章
  • Mutational effect of human CFAP43 splice-site variant causing multiple morphological abnormalities of the sperm flagella.

    abstract::Multiple morphological abnormalities of the sperm flagella (MMAF) is a rare disease associated with male infertility. In our previous study, we identified a homozygous CFAP43 splice-site variant, c.3661-2delA, in a patient with MMAF. However, the mutational effect of this variant was unknown. Here, using a minigene as...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13575

    authors: Li L,Feng F,Wang Y,Guo J,Yue W

    更新日期:2020-07-01 00:00:00

  • Association between leptin, obesity, hormonal interplay and male infertility.

    abstract::Male infertility is a major health problem worldwide. We investigated a possible association between leptin, obesity, hormonal interplay and male infertility. This cross-sectional study of 313 males (178 infertile and 135 fertile) was carried out in 2017. The subjects were categorised by body mass index (BMI) and body...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13147

    authors: Amjad S,Baig M,Zahid N,Tariq S,Rehman R

    更新日期:2019-02-01 00:00:00

  • Sperm selection strategies and their impact on assisted reproductive technology outcomes.

    abstract::The application of assisted reproductive technologies (ART) has revolutionised the treatment of human infertility, giving hope to the patients previously considered incapable of establishing pregnancy. While semen analysis is performed to access whether a sample has an adequate number of viable, motile and morphologic...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.13725

    authors: Pinto S,Carrageta DF,Alves MG,Rocha A,Agarwal A,Barros A,Oliveira PF

    更新日期:2020-06-28 00:00:00

  • Meningiomas in three male-to-female transgender subjects using oestrogens/progestogens and review of the literature.

    abstract::Sex hormones have been proposed as a possible risk factor for the development and growth of meningiomas. Hormonal therapy plays a fundamental role in the treatment of male-to-female transgenders and needs to be continued after sex reassignment surgery. Usually, this treatment leads to no adverse events; however, its i...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/and.12550

    authors: Ter Wengel PV,Martin E,Gooren L,Den Heijer M,Peerdeman SM

    更新日期:2016-12-01 00:00:00

  • The function of long noncoding RNA HOTAIRM1 in the progression of prostate cancer cells.

    abstract::Emerging evidence is indicating the importance of hundreds of long noncoding RNAs (lncRNAs) in the occurrence and progression of different malignancies such as prostate cancer (PCa). This study investigated the possible role of HOX transcript antisense intergenic RNA myeloid-specific 1 (HOTAIRM1) in the progression of...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13897

    authors: Wang L,Wang L,Wang Q,Yosefi B,Wei S,Wang X,Shen D

    更新日期:2020-12-23 00:00:00

  • Laparoscopy-assisted pelvi-scrotal vasovasostomy.

    abstract::Iatrogenic obstruction of the vas deferens within the inguinal canal can be managed by direct on-site vasovasostomy. However, in cases with large defect of the vas, the anastomosis may be under tension. Dissecting through the site of a previous hernia repair is tedious, and may lead to recurrence of the hernia. The pr...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2004.00624.x

    authors: Shaeer OK,Shaeer KZ

    更新日期:2004-10-01 00:00:00

  • HAART and anti-Koch's impair sexual competence, sperm quality and offspring quality when used singly and in combination in male Wistar rats.

    abstract::This study investigated the impact of the administration of HAART and anti-Koch's, singly and in combination, on sexual competence and birth statistics. Adult male Wistar rats were randomised into distilled water-treated control, HAART-treated, anti-Koch's-treated and HAART + anti-Koch's-treated groups. The 56-day ora...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13951

    authors: Akhigbe RE,Hamed MA,Odetayo AF

    更新日期:2021-01-05 00:00:00

  • Separation of sub-populations of sperm with higher fertility potential from normal and pathological semen by peanut agglutinin.

    abstract::Incubation of human sperm from semen with counts below 20 x 10(6)/ml with peanut agglutinin (PNA) resulted in agglutination of about 70% of sperm. An inverse correlation was found between non-agglutinating (PNA) sperm and sperm density (r = 0.48, p less than 0.01) and a direct correlation with acrosome-damaged sperm (...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1990.tb01970.x

    authors: Ravid A,Sagiv M,Bartoov B,Eltes F,Novogrodsky A,Allalouf D,Levinsky H,Singer R

    更新日期:1990-05-01 00:00:00

  • Leydig cell differentiation induced by stimulation with HCG and HMG in two patients affected with hypogonadotropic hypogonadism.

    abstract::In order to elucidate the ultrastructural characteristics of the precursor cell of Leydig cells and its subsequent differentiation, testicular biopsies from two patients with hypogonadotropic hypogonadism, obtained prior to and after treatment with HCG and HMG, were studied in comparison with those of prepubertal, pub...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1979.tb02187.x

    authors: Nistal M,Paniagua R

    更新日期:1979-05-01 00:00:00

  • Electrophoretic characterization of mouse epididymal esterases in inbred lines and in a natural population.

    abstract::Esterase isozymes were studied in mouse epididymis of two inbred strains (C57BL, DBA/2) and in a natural population (Swiss OF1), by using vertical polyacrylamide gel electrophoresis and staining with alpha or beta-naphthyl acetate as a substrate. Eighteen (C57BL), 17 (DBA/2) or 16 (Swiss OF1) epididymal isozymes were ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1986.tb01842.x

    authors: Abou-Haila A,Fain-Maurel MA

    更新日期:1986-11-01 00:00:00

  • The Estonian version of the National Institutes of Health chronic prostatitis symptom index.

    abstract::Our aim was to establish the Estonian version of the National Institutes of Health chronic prostatitis symptom index (NIH-CPSI), and to examine its validity and applicability in chronic prostatitis patients as well as in community-based study. The questionnaire was tested in 54 category III prostatitis patients. In ad...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2006.00723.x

    authors: Korrovits P,Punab M,Mehik A,Mändar R

    更新日期:2006-06-01 00:00:00

  • Evaluation of reference values of standard semen parameters in fertile Egyptian men.

    abstract::The reference values of human semen, published in the WHO's latest edition in 2010, were lower than those previously reported. The objective of this study was to evaluate reference values of standard semen parameters in fertile Egyptian men. This cross-sectional study included 240 fertile men. Men were considered fert...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12942

    authors: Zedan H,Ismail S,Gomaa A,Saleh R,Henkel R,Agarwal A

    更新日期:2018-01-15 00:00:00

  • Men regret anabolic steroid use due to a lack of comprehension regarding the consequences on future fertility.

    abstract::We examined whether men with anabolic-steroid-induced hypogonadism (ASIH) seeking testosterone supplementation therapy (TST) regretted their decision to use anabolic-androgenic steroids (AAS) and what their reasons were for this regret. An anonymous, prospective survey was distributed to 382 men seeking follow-up trea...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12340

    authors: Kovac JR,Scovell J,Ramasamy R,Rajanahally S,Coward RM,Smith RP,Lipshultz LI

    更新日期:2015-10-01 00:00:00

  • Androgen deficiency impairs erectile function in rats through promotion of corporal fibrosis.

    abstract::The aim of this study was to investigate the underlying mechanism of androgen deficiency inducing corporal fibrosis, thereby causing erectile dysfunction (ED). Forty 12-week-old healthy male rats were divided randomly into four groups: normal control group (Control); castration group (Castration); the other 20 rats we...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12797

    authors: Cui K,Li R,Chen R,Li M,Wang T,Yang J,Chen Z,Wang S,Liu J,Rao K

    更新日期:2018-02-01 00:00:00

  • The relationship between ejaculated ram sperm mitochondrial protein synthesis and motility.

    abstract::D-chloramphenicol, at concentrations of 20 and 40 micrograms/ml, inhibited over 80% of the newly synthesized mitochondrial proteins expressed by incorporation of 3H-amino acid mixture. At concentrations of 20 and 40 micrograms ml-1, D-chloramphenicol enhanced the collective motility of washed ram spermatozoa. The coll...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1994.tb00815.x

    authors: Twaina-Bechor E,Bartoov B

    更新日期:1994-11-01 00:00:00

  • Azoospermia and Sertoli-cell-only syndrome: hypoxia in the sperm production site due to impairment in venous drainage of male reproductive system.

    abstract::Sertoli-cell-only (SCO) syndrome, or germ cell aplasia, is diagnosed on testicular biopsy when germ cells are seen to be absent without histological impairment of Sertoli or Leydig cells. It is considered a situation of irreversible infertility. Recent studies have shown that varicocele, a bilateral disease, causes hy...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2010.01047.x

    authors: Gat Y,Gornish M,Perlow A,Chakraborty J,Levinger U,Ben-Shlomo I,Pasqualotto F

    更新日期:2010-10-01 00:00:00

  • Effects of continuous long-term testosterone therapy (TTh) on anthropometric, endocrine and metabolic parameters for up to 10 years in 115 hypogonadal elderly men: real-life experience from an observational registry study.

    abstract::Subnormal levels of testosterone are associated with significant negative health consequences, with higher risks of all-cause and cardiovascular mortality. The numbers of studies reporting on the benefits of normalisation of testosterone is increasing but longer-term data on (elderly) men receiving testosterone treatm...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12514

    authors: Yassin AA,Nettleship J,Almehmadi Y,Salman M,Saad F

    更新日期:2016-09-01 00:00:00

  • Experience with the Jonas penile prosthesis in the treatment of impotence.

    abstract::The penile prosthesis has become established as a mode of therapy for organic and chronic psychogenic impotence. The Jonas type of penile prosthesis has been implanted into 10 patients and has achieved both functional success and excellent acceptance by the partner. Mechanical and pathological complications have a ver...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1984.tb00277.x

    authors: Reisner GS,Lording DW,Rose N

    更新日期:1984-05-01 00:00:00

  • Mitochondria-targeted antioxidant SkQ1 improves spermatogenesis in Immp2l mutant mice.

    abstract::Previous studies have confirmed that spermatogenesis in homozygous Immp2l mutant male mice was normal at the age of 6 months, but was significantly abnormal at the age of 13 months. Meanwhile, oxidative stress is reported to be involved in spermatogenic impairment in old mutant mice. However, it is unclear whether ant...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12848

    authors: Jiang Y,Liu C,Lei B,Xu X,Lu B

    更新日期:2018-03-01 00:00:00

  • Upregulation of TRPV1 in spinal dorsal root ganglion by activating NGF-TrkA pathway contributes to pelvic organ cross-sensitisation in rats with experimental autoimmune prostatitis.

    abstract::Chronic prostatitis/chronic pelvic pain syndrome (CP⁄CPPS) is the prostate gland inflammation characterised as genitourinary pain in the pelvic region. The rat experimental autoimmune prostatitis (EAP) was achieved to mimic CP⁄CPPS. The expressions of transient receptor potential vanilloid 1 (TRPV1) in the prostate, b...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13302

    authors: Zhang J,Yi QT,Gong M,Zhang YQ,Liu D,Zhu RJ

    更新日期:2019-09-01 00:00:00

  • Deregulation of Aurora kinase gene expression in human testicular germ cell tumours.

    abstract::The Aurora kinases regulate chromosome segregation and cytokinesis, and alterations in their expression associate with cell malignant transformation. In this study, we demonstrated by qRT-PCR analysis of 14 seminomas that Aurora-A mRNA was, with respect to control tissues, augmented in five of 14 tumour tissues by 2.1...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2009.00987.x

    authors: Baldini E,Arlot-Bonnemains Y,Mottolese M,Sentinelli S,Antoniani B,Sorrenti S,Salducci M,Comini E,Ulisse S,D'Armiento M

    更新日期:2010-08-01 00:00:00

  • An immunohistochemical study for testicular biopsies in cases of male infertility.

    abstract::Immunohistochemical and morphological examination of 50 bilateral testicular biopsies of infertile men shows the presence of immunoglobulin in 20% of the cases, suggesting that testicular immune phenomena might be implicated in the pathogenesis of infertility. Several observations make it clear that immune phenomena a...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1980.tb00594.x

    authors: Jadot-Van De Casseye M,De Bled G,Gepts W,Schoysman R

    更新日期:1980-03-01 00:00:00

  • Physiological action of oestradiol on the acrosome reaction in human spermatozoa.

    abstract::The acrosome is a secretory vesicle located in the sperm head. The acrosome reaction consists in the fusion of the sperm plasma membrane with the external acrosomal membrane. It has been observed that this reaction does not take place in spermatozoa incubated in cervical mucus, hydrogel that contains high concentratio...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2007.00814.x

    authors: Vigil P,Toro A,Godoy A

    更新日期:2008-06-01 00:00:00

  • Dietary omega-3 polyunsaturated fatty acids induce plasminogen activator activity and DNA damage in rabbit spermatozoa.

    abstract::The aim of this study was to determine the effect(s) of dietary omega-3 polyunsaturated fatty acids (ω-3 PUFA) on rabbit semen. Adult rabbit bucks were assigned to two groups that were given two diets, a standard diet (control) and a diet supplemented with ω-3 PUFA. Sperm samples were collected from all bucks with the...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12776

    authors: Kokoli AN,Lavrentiadou SN,Zervos IA,Tsantarliotou MP,Georgiadis MP,Nikolaidis EA,Botsoglou N,Boscos CM,Taitzoglou IA

    更新日期:2017-12-01 00:00:00

  • Role of testicular tissue telomerase assay for the prediction of the presence of testicular spermatozoa in azoospermic men with varicoceles, pre- and post-varicocelectomy.

    abstract::We evaluated the reproductive potential of frozen/thawed testicular spermatozoa of azoospermic men with left varicocele. The role of testicular tissue telomerase assay (TTA) in the prediction of the presence of testicular spermatozoa pre- and post-varicocelectomy was investigated, as well. Therapeutic testicular biops...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.2004.00615.x

    authors: Giannakis D,Baltogiannis D,Tsoukanelis K,Loutradis D,Miyagawa I,Makrydimas G,Kanakas N,Sofikitis N

    更新日期:2004-06-01 00:00:00

  • Analysis of endothelial nitric oxide synthase (eNOS) G894T polymorphism and semen parameters in a Chinese Han population.

    abstract::Previous studies have shown that endothelial nitric oxide synthase (eNOS) gene may be involved in abnormal semen parameters. However, the relationship between eNOS G894T polymorphism and semen parameters remains controversial. The purpose of this study was to investigate the association of eNOS G894T polymorphism and ...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12113

    authors: Yu Q,Zhang Y,Xia Y,Yang X,Li N,Ye L,Mao X

    更新日期:2014-06-01 00:00:00

  • Secretory proteins of human seminal vesicles and their relationship to lipids and sugars.

    abstract::Proteins secreted by human seminal vesicles are strongly positively charged. Cellulose acetate electrophoresis show how the presence of two protein bands of vesicular origin (N3 and N4). When studied on SDS-PAGE there are three main bands of molecular weight 67, 45 and 40 kDa, respectively. These proteins may be separ...

    journal_title:Andrologia

    pub_type: 杂志文章,评审

    doi:10.1111/j.1439-0272.1990.tb02085.x

    authors: Montagnon D,Valtat B,Vignon F,Koll-Back MH

    更新日期:1990-01-01 00:00:00

  • The kinetic of acrosome reaction--an additional sperm parameter?

    abstract::Spermatozoa of six men with normozoospermia, oligozoospermia and teratozoospermia were capacitated in vitro in modified Tyrode's medium und evaluated for acrosome reaction using the recently described triple-stain technique. The resulting kinetics illustrate the different capacitation behaviour and ability of spermato...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/j.1439-0272.1985.tb00991.x

    authors: Töpfer-Petersen E,Heissler E,Schill WB

    更新日期:1985-05-01 00:00:00

  • mTOR expression in human testicular seminoma.

    abstract::The mammalian target of rapamycin (TOR) has been implicated in the control of different stressors, growth factors, nutrients and hormones, participating in the control of key cellular functions. Controlling this many pathways poses mTOR signalling as a potential new target in new treatment strategies for multiple canc...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.12504

    authors: Yaba A,Bozkurt ER,Demir N

    更新日期:2016-08-01 00:00:00

  • Low testicular zinc level, p53 expression and impairment of Sertoli cell phagocytosis of residual bodies in rat subjected to psychological stress.

    abstract::Psychological stress is a known aetiology of infertility. However, the mechanisms translating it to reproductive dysfunction are not fully elucidated. Three experiments were performed on Wistar rats were designed to evaluate Sertoli cell function under stress. In Experiment I, rats were randomised into three groups: s...

    journal_title:Andrologia

    pub_type: 杂志文章

    doi:10.1111/and.13958

    authors: Medubi LJ,Akinola OB,Oyewopo AO

    更新日期:2021-01-19 00:00:00