Abstract:
BACKGROUND & AIMS:We previously isolated a monoclonal antibody against a Necturus gallbladder epitope that blocks native adenosine 3',5'-cyclic monophosphate (cAMP)-dependent chloride channels in intestine, gallbladder, urinary bladder, and airway epithelia in various animals. METHODS:Using this antibody, we purified a 200-kilodalton protein that, when reconstituted in lipid bilayers, forms 9-pS chloride channels that are blocked by the antibody. RESULTS:Amino acid sequencing of the purified protein showed strong homology to rabbit sucrase-isomaltase, an abundant intestinal enzyme. Western blot analysis of the in vitro-translated sucrase-isomaltase was indistinguishable from that of the protein used in the lipid bilayer studies. Expression of this protein in Chinese hamster ovary cells and in Xenopus laevis oocytes yielded cAMP-dependent chloride currents that in the latter system were blocked by the antibody. CONCLUSIONS:Because the monoclonal antibody blocks cAMP-dependent currents in epithelia as well as those produced both by the reconstituted and by the heterologously expressed protein, sucrase-isomaltase is a cAMP-dependent epithelial chloride channel. Thus an enzyme that can also function as an ion channel has been described for the first time.
journal_name
Gastroenterologyjournal_title
Gastroenterologyauthors
Finn AL,Kuzhikandathil EV,Oxford GS,Itoh-Lindstrom Ydoi
10.1053/gast.2001.20884keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
117-25issue
1eissn
0016-5085issn
1528-0012pii
S0016508501465401journal_volume
120pub_type
杂志文章相关文献
GASTROENTEROLOGY文献大全abstract:BACKGROUND & AIMS:Fecal incontinence (FI) is characterized by uncontrolled passage of solid or liquid stool. We aimed to determine the prevalence and severity of FI in a large sample of US residents. METHODS:We recruited a representative sample of patients in October 2015 to complete the National Gastrointestinal (GI)...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2018.01.062
更新日期:2018-05-01 00:00:00
abstract::Incidence of intrahepatic arteriovenous fistula after needle biopsy of the liver, percutaneous transhepatic cholangiography, and transhepatic catheterization of an intrahepatic bile duct or portal vein, was determined by studying the hepatic arteriograms obtained within 1 month of the transhepatic procedure. An arteri...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-06-01 00:00:00
abstract::A patient who developed obstructive jaundice as a complication of a liver biopsy is presented. The jaundice was found to be attributable to obstruction of the biliary tree at the level of the bifurcation by an intrahepatic hematoma. The patient had a complete and spontaneous recovery. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract:BACKGROUND & AIMS:Apart from its importance as an acid secretogogue, the role of histamine as a downstream target of gastrin has not been fully explored. Previous studies have shown that the combination of hypergastrinemia and Helicobacter infection resulted in accelerated gastric cancer in mice. We used this model to ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2005.03.027
更新日期:2005-06-01 00:00:00
abstract::We have examined the receptor binding of radio-iodinated murine epidermal growth factor and the effects of epidermal growth factor on both amylase release and incorporation of [3H]leucine into protein in isolated adult mouse pancreatic acini in primary culture. Binding of radioiodinated murine epidermal growth factor ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::Partial obstruction of the opossum esophagus leads to thickening of the circular muscle, hypertrophy of smooth muscle cells, and diminution of the extracellular space. The pharmacological and electrophysiological properties of this hypertrophied muscle were studied. Carbachol produced phasic and tonic contractions of ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)90522-m
更新日期:1991-09-01 00:00:00
abstract::Exogenous administration of substances extracted from rodent salivary glands may increase the resistance of gastric mucosal barrier to the disruptive actions of ulcerogenic agents and/or reduce acid secretory output. In the present study the influence of desalivation in the rat on acid secretory function and mucosal i...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1981-08-01 00:00:00
abstract::The possibility that chronic uremia renders the gastric mucosa more susceptible to acid injury was investigated. A rat model of chronic renal failure was induced by subtotal nephrectomy. [H+] back-diffusion across the mucosa, following intragastric perfusion of 0.15N HCl or 15% ethanol in 0.15N HCl, was significantly ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91432-4
更新日期:1992-12-01 00:00:00
abstract:BACKGROUND & AIMS:Incidence of and mortality from pancreatic ductal adenocarcinoma (PDAC), the most common form of pancreatic cancer, are almost equivalent, so better treatments are needed. We studied gene expression profiles of PDACs and the functions of genes with altered expression to identify new therapeutic target...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2016.08.040
更新日期:2016-12-01 00:00:00
abstract:BACKGROUND/AIMS:The pathways underlying rectal hypersensitivity to balloon distention in patients with irritable bowel syndrome (IBS) are not known. The aim of this study was to characterize the involvement of sacral and thoracolumbar afferents in the perception of rectal distention. METHODS:Rectal balloon distention ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(94)90809-5
更新日期:1994-12-01 00:00:00
abstract:BACKGROUND & AIMS:In colorectal adenoma and carcinoma, glutathione S-transferase-pi (GSTP1-1) is highly expressed. K-ras mutation is also known to occur frequently in colorectal adenoma and carcinoma, as well as in the putative precursor of adenoma, aberrant crypt foci (ACF). Further, forced expression of v-H-ras in ra...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/gast.2001.27982
更新日期:2001-10-01 00:00:00
abstract:BACKGROUND & AIMS:The effect of endogenous hypergastrinemia on growth of human colon carcinoma is not known. Our aim was to study the growth of human colon carcinoma in an animal model with endogenous hypergastrinemia. METHODS:Human colon carcinoma was transplanted to the colon of 40 athymic rats. Of these, 25 underwe...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(95)90625-8
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND & AIMS:Oral administration of ursodeoxycholic acid (UDCA) and cholesterol causes bile salt malabsorption; the former by competition for and the latter by down-regulation of ileal bile acid transporters. Because ileectomy in rats induces enterohepatic cycling of bilirubin, the hypothesis that dietary steroids...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(98)70152-0
更新日期:1998-09-01 00:00:00
abstract::Median nerve somatosensory evoked potentials were recorded from 33 patients with various degrees of hepatic failure and from 10 age-matched controls. Within 20 ms poststimulation, one negative peak (N13) could be recorded from the middle of the back of the patient's neck at the C2 vertebral level. Within 150 ms, three...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90460-3
更新日期:1985-09-01 00:00:00
abstract::Hepatitis C virus (HCV) is the agent responsible for posttransfusion hepatitis. The incidence, timing, and clinical course of HCV positive hepatitis in liver transplant recipients are unknown. Three hundred and seventeen donor-recipient liver transplant pairs were grouped on the basis of their pretransplant HCV antibo...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91130-v
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND & AIMS:Current stool DNA tests identify about half of individuals with colorectal cancers and miss most individuals with advanced adenomas. We developed a digital melt curve (DMC) assay to quantify low-abundance mutations in stool samples for detection of colorectal neoplasms and compared this test with othe...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2008.10.023
更新日期:2009-02-01 00:00:00
abstract:BACKGROUND & AIMS:We have shown previously that cure of Helicobacter pylori infection leads to the disappearance of acid-neutralizing substances. Also, patients with ulcer after cure may gain weight. The aim of this study was to investigate whether cure of the infection increases the risk of reflux esophagitis. METHOD...
journal_title:Gastroenterology
pub_type: 临床试验,杂志文章
doi:10.1016/s0016-5085(97)70024-6
更新日期:1997-05-01 00:00:00
abstract:BACKGROUND & AIMS:Deoxycholate (DC) has proapoptotic and tumorigenic effects in different cell types of the gastrointestinal tract. Exposure of BHK-21 (stromal) cells to DC induces Ca(2+) entry at the plasma membrane, which affects intracellular Ca(2+) signaling. We assessed whether DC-induced increases in [Ca(2+)] can...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2009.03.038
更新日期:2009-09-01 00:00:00
abstract::Among the components of dietary fiber, the soluble polysaccharides, primarily guaran and pectin, have been found to impair intestinal absorption. Little is known, however, about the mechanism of this effect. The direct action of guaran on small intestinal absorption was evaluated by a single-pass perfusion technique. ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::The study of cholesterol gallstone disease would be facilitated if the nucleation time of cholesterol crystals could be measured in duodenal bile and was correlated with nucleation occurring in vivo. Therefore, our aims were to determine (a) if nucleation time could be measured in duodenal bile, (b) the effect of bact...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(91)90480-9
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND & AIMS:We aimed to identify the incidence and predictors of de novo gastroesophageal variceal formation and progression in a large cohort of patients with chronic hepatitis C and advanced fibrosis. METHODS:All participants in the Hepatitis C Antiviral Long-Term Treatment against Cirrhosis Trial were offered...
journal_title:Gastroenterology
pub_type: 杂志文章,随机对照试验
doi:10.1053/j.gastro.2010.02.058
更新日期:2010-06-01 00:00:00
abstract::Characteristics of electrical activities, and the relationship between electrical and motor activities, were studied in circular and longitudinal (taenia) muscle of the human colon that was obtained from 21 individuals. Recordings were obtained with suction electrodes, the sucrose-gap method, and microelectrodes. The ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(85)90513-x
更新日期:1985-02-01 00:00:00
abstract::Intracellular microelectrodes were used to evaluate electrical properties of the cell membranes in Necturus antral mucosa during exposure to luminal acid alone (pH 4) or to 5 mmol/L aspirin [acetylsalicylic acid (ASA)] in the presence of luminal acid. When nutrient solutions were buffered by HCO3- (pH 7.3), ASA modera...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91167-3
更新日期:1992-11-01 00:00:00
abstract:BACKGROUND & AIMS:Treatment options are limited for patients with hepatitis C (HCV) infection with treatment failure after sofosbuvir plus an NS5A inhibitor. There are some data for the efficacy of glecaprevir/pibrentasvir (G/P) in these patients. We performed a randomized trial of the safety and efficacy of 12 and 16 ...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1053/j.gastro.2019.08.008
更新日期:2019-12-01 00:00:00
abstract::Using in vivo and in vitro techniques, the mechanism by which intestinal mucosa obtains glutamine from luminal oligopeptides was investigated in humans. The rate of hydrolysis by mucosal brush border membrane was more than threefold greater for alanylglutamine than for glycylglutamine. Despite this difference, rates o...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/0016-5085(92)91088-l
更新日期:1992-07-01 00:00:00
abstract:BACKGROUND & AIMS:The mechanisms for tissue regeneration and renewal after acute pancreatitis are not well understood but may involve activation of Notch signaling. To study the effect of Notch signaling ablation during acute experimental pancreatitis, we used a chemical and genetic approach to ablate Notch signaling i...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1053/j.gastro.2007.11.003
更新日期:2008-02-01 00:00:00
abstract:BACKGROUND & AIMS:Narrow-band imaging (NBI) allows real-time histologic classification of colorectal polyps. We investigated whether endoscopists without prior training in NBI can achieve the following thresholds recommended by the American Society for Gastrointestinal Endoscopy: for diminutive colorectal polyps charac...
journal_title:Gastroenterology
pub_type: 杂志文章,多中心研究
doi:10.1053/j.gastro.2015.10.042
更新日期:2016-02-01 00:00:00
abstract:BACKGROUND & AIMS:Impaired gallbladder emptying occurs in patients undergoing bile salt therapy for cholesterol gallstone dissolution and in patients with cirrhosis who have elevated serum bile salt concentrations. To determine if bile salts directly inhibit gallbladder contractility, isometric contraction of the guine...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:10.1016/s0016-5085(97)70053-2
更新日期:1997-05-01 00:00:00
abstract:DESCRIPTION:This expert review was commissioned and approved by the AGA Institute Clinical Practice Updates Committee and the AGA Governing Board to provide timely guidance on a topic of high clinical importance to the AGA membership. The intent is to evaluate the current data on mechanism of altered coagulation in pat...
journal_title:Gastroenterology
pub_type: 杂志文章,实务指引,评审
doi:10.1053/j.gastro.2019.03.070
更新日期:2019-07-01 00:00:00
abstract::The effects of chronic mild restraint on the susceptibility of the rat gastric mucosa to ethanol or cold-restraint injury were studied. Gastric mucosas of animals subjected to chronic mild restraint exhibited less damage when bathed with 40% ethanol than those of control animals. This reduced damage was observed with ...
journal_title:Gastroenterology
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00