Abstract:
:We generated a new cell line, N38, by conditionally immortalizing mouse oligodendrocytes (OLs) at early stages of maturation. The morphology and marker expression pattern suggest N38 cells are similar to immature OLs. N38 cells were sensitive to changes in serum concentrations, and forcing the cells to differentiate in low serum at 39 degrees C significantly decreased the survival of the cells. Importantly, addition of PDGFaa, bFGF or astrocyte-conditioned medium had protective effects on the cells, by increasing cell proliferation but not cell differentiation. This effect was receptor-mediated. Exposure of N38 cells to differentiating signals such as retinoic acid did not cause further differentiation of the cells. The N38 cell line expresses the vertebrate homolog of the Drosophila notch-1 receptor, a molecule that appears to regulate OL differentiation. Notch-1 receptor was homogeneously distributed in the somas of N38 cells. Incubation of N38 cells with either PDGFaa or bFGF, however, induced a polarized distribution of the receptor in the majority of the cells as well as an upregulation of receptor protein levels. The upregulation of molecules, such the notch-1 receptor, in pathways that control differentiation might be an important mechanism for keeping OL precursors in an undifferentiated state during their exit of the germinal layer and migration in the developing central nervous system. This OL cell line might constitute a suitable model for studies of regulatory mechanisms at this stage of OL differentiation.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Bongarzone ER,Byravan S,Givogri MI,Schonmann V,Campagnoni ATdoi
10.1002/1097-4547(20001101)62:3<319::AID-JNR1>3.0.keywords:
subject
Has Abstractpub_date
2000-11-01 00:00:00pages
319-28issue
3eissn
0360-4012issn
1097-4547pii
10.1002/1097-4547(20001101)62:3<319::AID-JNR1>3.0.journal_volume
62pub_type
杂志文章abstract::The osteoinductive capacity of biological noncellular material has been widely recognized. Studies using bone morphogenetic proteins and acellular bone matrix demonstrate that host mesenchymal cells can be readily transformed into osteoprogenitor cells. The current study sought to determine whether another biological ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23505
更新日期:2015-03-01 00:00:00
abstract::Inhibitory luminopsins (iLMO2) integrate opto- and chemo-genetic approaches and allow for cell-type specific inhibition of neuronal activity. When exposed to a Renilla luciferase substrate, Coelenterazine (CTZ), iLMO2 generates bioluminescence-mediated activation of its amino-terminal halorhodopsin, resulting in neuro...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24109
更新日期:2020-03-01 00:00:00
abstract::Neural expression of constitutive hsc70 mRNA and hyperthermia-inducible hsp70 mRNA is examined using radioactive and non-radioactive in situ hybridization procedures. A strong induction of hsp70 mRNA was noted in cell populations in cerebellar layers and in the brainstem which demonstrated expression of mRNA encoding ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410506
更新日期:1995-08-01 00:00:00
abstract::Prothrombin and its active derivative thrombin are key members of the coagulation system. The only site of extra-hepatic thrombin expression is the central nervous system (CNS), where it is involved in brain development, protection, and regeneration. Thrombin affects various degenerative and ischemic CNS diseases like...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24049
更新日期:2017-10-01 00:00:00
abstract::To identify and characterize the lineage potential of rat neural retina progenitor cells (NRPCs) in vitro and engrafted into rats with retinal degeneration, NRPCs were isolated from neural retinas of embryonic day 17 Long Evans rats and cultured in serum-free or serum-containing media with fibroblast growth factor 2 a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10320
更新日期:2002-08-15 00:00:00
abstract::Molecular cloning has introduced an unexpected, large diversity of neurotransmitter hetero- oligomeric receptors. Extensive research on the molecular structure of the γ-aminobutyric acid receptor (GABAR) has been of great significance for understanding how the nervous system works in both vertebrates and invertebrates...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23695
更新日期:2016-02-01 00:00:00
abstract::Corticosterone (CORT), one of the glucocorticoids, causes neuronal damage in the hippocampus, but the mechanism(s) of action underlying its effects remains unknown. Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor that belongs to the neurotrophin family, affects the survival and/or differentiation of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990715)57:2<227::AID-JNR
更新日期:1999-07-15 00:00:00
abstract::Localization of acetylcholine receptors (AChRs) to the postsynaptic region of muscle is mediated in part by transcriptional mechanisms, because the genes encoding AChR subunits are transcribed selectively in synaptic myofiber nuclei. Neuregulin-1 (NRG-1) is a synaptic signal and induces transcription of AChRs in muscl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21563
更新日期:2008-04-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a late-onset progressive neurodegenerative disease affecting motor neurons. About 2% of patients with the disease are associated with mutations in the gene encoding Cu/Zn superoxide dismutase (SOD1). The purpose of this study is to assess the effect of rosemary extract and its ma...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22242
更新日期:2010-03-01 00:00:00
abstract::Heme oxygenase-1 (HO-1) is a stress protein induced in response to a variety of oxidative challenges. After treatment of the hybrid septal cells SN 56 with beta-amyloid peptide (beta-AP1-40) or hydrogen peroxide (H2O2), we detected high levels of reactive oxygen species, accompanied by a significant elevation in HO-1 ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990615)56:6<652::AID-JNR
更新日期:1999-06-15 00:00:00
abstract::In order to quantify the changes that occur in the cholinergic central nervous system with aging, we have compared acetylcholine (Ach) formation in brain cortex slice preparations from 2-year-old aged CBF-1 mouse brains and compared the findings with those in 2-4-month-old young adult mouse brain slices. Incorporation...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150209
更新日期:1986-01-01 00:00:00
abstract::Transplanting neural stem cells (NSC) to the damaged brain has been regarded as a potential treatment for neurodegenerative diseases such as Alzheimer's disease (AD), a condition characterized by memory loss. We hypothesized that transplantation of NSC into the hippocampal regions of APP + PS1 transgenic (Tg) mice, a ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23299
更新日期:2014-02-01 00:00:00
abstract::CD9 is a tetra-membrane-spanning glycoprotein involved in cell adhesion, migration, and proliferation in both the immune and the immature nervous system. In this study, CD9 expression was detected in myelin of mouse brain, starting at postnatal day 16. The amount of CD9 protein continuously increased with age and pers...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19971015)50:2<312::AID-JNR
更新日期:1997-10-15 00:00:00
abstract::The pathogenic processing of the amyloid precursor protein (APP) into beta-amyloid peptides, which give rise to beta-amyloid plaques in the brains of Alzheimer's disease patients, requires the enzymatic activity of the beta-site APP-cleaving enzyme 1 (BACE1). We report the cloning and sequence of a 1.5-kb DNA fragment...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10639
更新日期:2003-07-01 00:00:00
abstract::Only a few proteins are known to be exclusively expressed in central nervous system (CNS) myelin. A novel surface membrane protein expressed only in CNS myelin and oligodendrocytes of higher vertebrates has been identified by a monoclonal antibody. This CNS myelin/oligodendrocyte-specific protein, MOSP, has a molecula...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490280418
更新日期:1991-04-01 00:00:00
abstract::Brain cathepsin D, purified by affinity chromatography on Sepharose pepstatin columns, was incubated with synthetic peptides corresponding to the susceptible regions of the myelin basic protein encompassing the two Phe-Phe bonds. One peptide, Leu-Gly-Arg-Phe-Phe-Gly-Gly, was cleaved by cathepsin D at the Phe-Phe bond ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490050306
更新日期:1980-01-01 00:00:00
abstract::Astrocytes are glial cells that are distributed throughout the central nervous system in an arrangement optimal for chemical and physical interaction with neuronal synapses and brain blood supply vessels. Neurotransmission modulates astrocytic excitability by activating an array of cell surface receptors and transport...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24075
更新日期:2017-12-01 00:00:00
abstract::Experimental autoimmune encephalomyelitis (EAE) is an inflammatory demyelinating disease with similarities to multiple sclerosis that requires the activation of auto reactive T cells that infiltrate the central nervous system. In previous studies we have shown that intraperitoneal administration of synaptosomal antige...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22048
更新日期:2009-08-01 00:00:00
abstract::To investigate the function of the dipeptide carnosine in the olfactory system, alterations of carnosine in the primary olfactory nerve after unilateral naris closure or olfactory bulb semilesion were studied by means of immunocytochemistry. After unilateral naris closure, carnosine staining showed no alteration, but ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490340608
更新日期:1993-04-15 00:00:00
abstract::Alterations in the 5-HT2A receptor gene expression in the prefrontal cortex have been suggested to play a role in the pathophysiology and pharmacotherapy of schizophrenia. This study measured mRNA encoding 5-HT2A receptor in the left superior frontal gyrus from chronic elderly schizophrenics (n = 21) with varying neur...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:2000-01-15 00:00:00
abstract::Gamma-Aminobutyric acid (GABA) is the principal inhibitory neurotransmitter in the brain. A deficiency of GABAergic inhibition mediated via the GABAA receptor complex has for a long time been suspected to be a central factor in epileptogenesis. Status epilepticus is a condition of sustained and prolonged excitation of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19981215)54:6<863::AID-JNR
更新日期:1998-12-15 00:00:00
abstract::The mammalian genome contains both constitutively expressed and heat-shock-inducible members of the hsp70 gene family. Riboprobes derived from members of these two classes of heat shock genes were utilized in Northern blot studies to analyze brain mRNA isolated from control rabbits and rats and from animals subjected ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250103
更新日期:1990-01-01 00:00:00
abstract::Recent studies have revealed a significant decrease in white matter volume, including loss of myelin, with age but minimal decrease in gray matter volume (Guttmann et al., [1998] Neurology 50:972-978). Myelin is necessary for the rapid conduction of impulses along axons. Myelinated nerve includes various domains, the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10386
更新日期:2002-11-01 00:00:00
abstract::This investigation explores the central nervous system protein-synthetic responses to hemisection of the spinal cord. Adult male rats hemisected at mid T2 were given intravenous injections of 14C-leucine at 0.7, 30, or 70 days postoperative. Four hours later the T1, T2, T3, and C6 segments of the spinal cord and right...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010107
更新日期:1975-01-01 00:00:00
abstract::It was previously reported that a monoclonal antibody to beta-amyloid precursor protein (mab22C11; Boehringer Mannheim, Indianapolis, IN) labels an intermediate filament-associated protein (beta APP-IFAP) in cultured human skin fibroblasts (Dooley et al.: J Neurosci Res 33:60-67, 1992). The time course of its expressi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490380605
更新日期:1994-08-15 00:00:00
abstract::Recent data indicate that molecular mimicry may play a role in the pathogenesis of human-T-lymphotropic virus type-1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP), an immune-mediated disease of the central nervous system (CNS). Specifically, HAM/TSP patients developed antibodies that cross-reac...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20137
更新日期:2004-07-01 00:00:00
abstract::Preterm birth results in significant cognitive and motor disabilities, but recent evidence suggests that there is variable recovery over time. One possibility that may explain this variable recovery entails variable neurogenic responses in the subventricular zone (SVZ) following the period of chronic hypoxia experienc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21597
更新日期:2008-05-01 00:00:00
abstract::The effects of cations on the spontaneous and potassium-stimulated taurine release from mouse cerebral cortex slices were assessed with an emphasis on the as yet unestablished calcium dependence of the stimulated release. Spontaneous and stimulated GABA release was analyzed for comparison. A depolarizing concentration...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170316
更新日期:1987-01-01 00:00:00
abstract::The multifactorial nature of traumatic brain injury (TBI), especially the complex secondary tissue injury involving intertwined networks of molecular pathways that mediate cellular behavior, has confounded attempts to elucidate the pathology underlying the progression of TBI. Here, systems biology strategies are explo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23503
更新日期:2015-02-01 00:00:00
abstract::The inflammatory response initiated after spinal cord injury (SCI) is characterized by the accumulation of macrophages at the impact site. Monocyte chemoattractant protein-1 (MCP-1) is a strong candidate for mediating chemotaxis of monocytes to the injured nervous system. To help in defining the role of MCP-1 in infla...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10269
更新日期:2002-06-15 00:00:00