Abstract：
:In addition to its role in the uptake of apolipoprotein B (apoB)-containing lipoproteins, apoE promotes hepatic very low density lipoprotein-triglyceride (VLDL-TG) production in animal models. However, it is not known if apoE increases the amount of TG per VLDL particle or the number of VLDL particles secreted. VLDL-apoB production is a measure of the rate of VLDL particle secretion. We determined the effects of apoE deficiency and apoE overexpression on VLDL-apoB production in mice. [(35)S]methionine was injected into endogenously label VLDL-apoB and Triton WR-1339 was simultaneously injected to block the catabolism of VLDL. Compared with wild-type mice, the VLDL-apoB production rate was decreased by 33% in apoE-deficient mice. Conversely, VLDL-apoB production was increased by 48% in mice overexpressing apoE compared with controls. Nascent VLDL, obtained from post-Triton plasma, had a decreased, not increased, content of TG per apoB in the apoE-overexpressing group compared with the control group. This study demonstrates that hepatic apoE expression increases the output of VLDL triglyceride by increasing the production rate of VLDL-apoB, suggesting that hepatic apoE influences the number of VLDL particles secreted by the liver.
journal_name
J Lipid Resjournal_title
Journal of lipid researchauthors
Maugeais C,Tietge UJ,Tsukamoto K,Glick JM,Rader DJkeywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
1673-9issue
10eissn
0022-2275issn
1539-7262journal_volume
41pub_type
杂志文章abstract：:A group of polyunsaturated fatty acids called conjugated linoleic acids (CLAs) are found in ruminant products, where the most common isomers are cis9, trans11 (c 9,t11) and trans10, cis12 (t10,c12) CLA. A crude mixture of these isomers has been shown in animal studies to alter body composition by a reduction in body f...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M300120-JLR200
更新日期：2003-08-01 00:00:00
abstract：:ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty acids. There are two known genes encoding the two ACAT enzymes, ACAT1 and ACAT2 (also known as Soat1 and Soat2). In adult humans, ACAT1 is present in most tissues, whereas ACAT2 is localized to enterocytes and hepatocytes. In this...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M400450-JLR200
更新日期：2005-09-01 00:00:00
abstract：:Sebum is a complex lipid mixture that is synthesized in sebaceous glands and excreted on the skin surface. The purpose of this study was the comprehensive detection of the intact lipids that compose sebum. These lipids exist as a broad range of chemical structures and concentrations. Sebum was collected with SebuTape(...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.D008391
更新日期：2010-11-01 00:00:00
abstract：:The conversion of alpha-linolenate (18:3n-3) to docosahexaenoate (22:6n-3) in the presence of low and high dietary levels of linoleate (18:2n-6) is reported in young rats using [U-13C]-alpha-linolenic acid (18:2n-3*) and high precision gas chromatography-combustion isotope ratio mass spectrometry (GCC-IRMS). After con...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1995-05-01 00:00:00
abstract：:Two novel mutations of the low density lipoprotein (LDL)-receptor gene were found in two Italian familial hypercholesterolemia (FH)-heterozygotes. The first mutation was an 18 nucleotide duplication in exon 8 which is preceded by an A-->T transversion. The translation product of the mutant allele was predicted to be a...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1998-07-01 00:00:00
abstract：:We have studied the synthesis, modification, and secretion of rat apoE in primary cultures of neonatal aortic smooth muscle cells and adult rat hepatocytes. The cultures were pulsed with [35S]methionine and the intracellular and secreted apoE were immunoprecipitated and analyzed by two-dimensional isoelectric focusing...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:The mammalian FA-binding proteins (FABPs) bind long-chain FA with high affinity. The large number of FABP types is suggestive of distinct functions in specific tissues. Multiple experimental approaches have shown that individual FABPs possess both unique and overlapping functions, some of which are based on specific e...
journal_title：Journal of lipid research
pub_type： 杂志文章,评审
doi：10.1194/jlr.R800084-JLR200
更新日期：2009-04-01 00:00:00
abstract：:Lysophospholipid (LP) research has experienced a period of renaissance with the discovery of the lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) receptors in the late 1990s. Vertebrate LP receptors regulate embryogenesis, vascular development, neurogenesis, uterine development, oocyte survival, immune ce...
journal_title：Journal of lipid research
pub_type： 杂志文章,评审
doi：10.1194/jlr.R800047-JLR200
更新日期：2009-04-01 00:00:00
abstract：:Rat serum lipoproteins were separated into at least four fractions by agarose-starch gel electrophoresis. The system used was discontinuous in that glycine and sodium barbitone buffer was used in the reservoirs and Tris buffer was used for the gels. The four major bands could be related to the pattern obtained by ultr...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1971-05-01 00:00:00
abstract：:Apolipoproteins, such as apolipoprotein A-I (apoA-I), can stimulate cholesterol efflux from cells expressing the ATP binding cassette transporter A1 (ABCA1). The nature of the molecular interaction between these cholesterol acceptors and ABCA1 is controversial, and models suggesting a direct protein-protein interactio...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M300355-JLR200
更新日期：2004-02-01 00:00:00
abstract：:Sterol-regulatory element binding protein-1c (SREBP-1c) is a transcription factor that controls lipogenesis in the liver. Hepatic SREBP-1c is nutritionally regulated, and its sustained activation causes hepatic steatosis and insulin resistance. Although regulation of SREBP-1c is known to occur at the transcriptional l...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M004234
更新日期：2010-07-01 00:00:00
abstract：:Genetic defects in SAR1B GTPase inhibit chylomicron (CM) trafficking to the Golgi and result in a huge intraenterocyte lipid accumulation with a failure to release CMs and liposoluble vitamins into the blood circulation. The central aim of this study is to test the hypothesis that SAR1B deletion (SAR1B-/- ) disturbs e...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.RA119000119
更新日期：2019-10-01 00:00:00
abstract：:Hepatocytes and hepatic stellate cells play important roles in retinoid storage and metabolism. Hepatocytes process postprandial retinyl esters and are responsible for secretion of retinol bound to retinol-binding protein (RBP) to maintain plasma retinol levels. Stellate cells are the body's major cellular storage sit...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：2000-06-01 00:00:00
abstract：:3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the enzyme catalyzing the rate-limiting step in cholesterol biosynthesis, exists in one active (dephosphorylated) and one inactive (phosphorylated) form in liver microsomes obtained from several animal species. The present study was undertaken in order to dete...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1984-11-01 00:00:00
abstract：:Genetic linkage analysis in the laboratory mouse identified chromosomal regions containing genes that contribute to cholesterol accumulation in the liver and plasma. Comparisons between five inbred strains of mice obtained from the Jackson Laboratory (DBA/2, AKR, C57BL/6, SJL, and 129P3) revealed a direct correlation ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:Cystic fibrosis (CF) patients and model systems exhibit consistent abnormalities in PUFA metabolism, including increased metabolism of linoleate to arachidonate. Recent studies have connected these abnormalities to increased expression and activity of the Δ6- and Δ5-desaturase enzymes. However, the mechanism connectin...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M050369
更新日期：2014-07-01 00:00:00
abstract：:In humans, peroxisomes harbor a complex set of enzymes acting on various lipophilic carboxylic acids, organized in two basic pathways, alpha-oxidation and beta-oxidation; the latter pathway can also handle omega-oxidized compounds. Some oxidation products are crucial to human health (primary bile acids and polyunsatur...
journal_title：Journal of lipid research
pub_type： 杂志文章,评审
doi：10.1194/jlr.R005959
更新日期：2010-10-01 00:00:00
abstract：:We describe new methods for analyzing the apolipoproteins (apo) of the high density lipoproteins (HDL) of several species by two modes of capillary electrophoresis: size separation using a molecular sieving buffer, and capillary zone electrophoresis (CZE) using neutral coated capillaries. By either mode HDL apos were ...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Expression of constitutively activated Akt in the mammary glands of transgenic mice results in a delay in post-lactational involution. We now report precocious lipid accumulation in the alveolar epithelium of mouse mammary tumor virus-myr-Akt transgenic mice accompanied by a lactation defect that results in a 50% decr...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M300045-JLR200
更新日期：2003-06-01 00:00:00
abstract：:EPA and DHA are not biologically equivalent; however, their individual activity on B cells is unknown. We previously reported fish oil enhanced murine B-cell activity in obesity. To distinguish between the effects of EPA and DHA, we studied the ethyl esters of EPA and DHA on murine B-cell function as a function of tim...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M049809
更新日期：2014-07-01 00:00:00
abstract：:The acute-phase response (APR) leads to alterations in lipid metabolism and type II nuclear hormone receptors, which regulate lipid metabolism, are suppressed, in liver, heart, and kidney. Here, we examine the effect of the APR in adipose tissue. In mice, lipopolysaccharide produces a rapid, marked decrease in mRNA le...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M500540-JLR200
更新日期：2006-10-01 00:00:00
abstract：:Gap junctions of rat brown adipose tissue (BAT) were studied with the freeze-fracture technique during development. The frequency and the mean area of gap junctions increased after birth, reached a maximal development during the functional phase of the tissue (birth to 3--4 weeks) and decreased while brown adipose tis...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
abstract：:X-linked adrenoleukodystrophy (X-ALD) is a peroxisomal disorder caused by impaired degradation of very long-chain fatty acids (VLCFAs) due to mutations in the ABCD1 gene responsible for VLCFA transport into peroxisomes. Lorenzo's oil, a 4:1 mixture of glyceryl trioleate and glyceryl trierucate, has been used to reduce...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M044586
更新日期：2014-03-01 00:00:00
abstract：:MDCO-216, a complex of dimeric recombinant apoA-IMilano (apoA-IM) and palmitoyl-oleoyl-phosphatidylcholine (POPC), was administered to cynomolgus monkeys at 30, 100, and 300 mg/kg every other day for a total of 21 infusions, and effects on lipids, (apo)lipoproteins, and ex-vivo cholesterol efflux capacity were monitor...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M033779
更新日期：2013-09-01 00:00:00
abstract：:The purpose of the present study was to examine the regulation of levels of apolipoprotein B (apoB) mRNA and its protein by cytokines in HepG2 cells. A dose-dependent increase in apoB mRNA levels was observed in the presence of either interleukin-1beta (IL-1beta) or IL-6 alone. This increase occurred as early as 1 h a...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:A relatively rapid procedure is described for the spectrophotometric determination of total tocopherol in red blood cells (RBC) based on a modification of the original Emmerie-Engel reaction. The critical feature in this method is the presence of a large amount of an added antioxidant, pyrogallol or ascorbic acid, dur...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1973-09-01 00:00:00
abstract：:Evidence is given that the heart of the cardiomyopathic UM-X7.1 hamster has a lipid composition different from that of the same tissue isolated from animals of the Syrian hamster parent strain. Also, noncardiac tissues from cardiomyopathic and healthy hamsters exhibit significant compositional differences. On the basi...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Three pentadecynoic acids, with the triple bond in the 8-, 9-, and 10-positions, have been synthesized on a gram scale in over-all yield of 65% by refinements of the five-step Ahmad-Strong method; isolation of intermediates was shown to be unnecessary prior to purification of the acetylenic nitriles by column chromato...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：
更新日期：1969-11-01 00:00:00
abstract：:Whereas antibipolar drug administration to rats reduces brain arachidonic acid turnover, excessive N-methyl-d-aspartate (NMDA) signaling is thought to contribute to bipolar disorder symptoms and may increase arachidonic acid turnover in rat brain phospholipids. To determine whether chronic NMDA would increase brain ar...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M700406-JLR200
更新日期：2008-01-01 00:00:00
abstract：:The pathogenesis of aortic valve stenosis (AS) is characterized by the accumulation of LDL-derived cholesterol in the diseased valves. Since LDL particles also contain plant sterols, we investigated whether plant sterols accumulate in aortic valve lesions. Serum samples were collected from 82 patients with severe AS a...
journal_title：Journal of lipid research
pub_type： 杂志文章
doi：10.1194/jlr.M800058-JLR200
更新日期：2008-07-01 00:00:00
